Difference between revisions of "Team:FAFU-CHINA/Composite Part"

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<div>
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<div style="height:auto!important;margin-left:auto;margin-right:auto;min-height:841.9pt;padding-bottom:1pt;padding-top:1pt">     
</p>
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<div style="margin-left:auto;margin-right:auto;position:relative"><p style="text-align:left"><a name="_GoBack"></a><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry4Aa4+Extended FMDV 2A</span><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">-pChlamy_3<span></span></span></p>
  
<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
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  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry4Aa4+Extended FMDV 2A<span></span></span></p>
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    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
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    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in DH5</span><span style="font-family:Calibri;font-size:11.0pt">α</span><span style="font-family:Calibri;font-size:11.0pt"><span></span></span></p>
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    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">3673bp<span></span></span></p>
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    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
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    </td>  </tr></tbody>
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</table>
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<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
  
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<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
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  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
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    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
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    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
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    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
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    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
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    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
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      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
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    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
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    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
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    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
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    </td>  </tr></tbody>
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</table>
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<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
  
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<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
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  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="263.20001220703125" src="http://b263.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/yfm7rDaljwf3tLDwN85uBcwarSLNpdnfMlUxh1G7pP4!/b/dAcBAAAAAAAA&amp;bo=sAAIAQAAAAADAJw! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
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      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="294.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/O5V87mM7vgBQcBKlBbjOuO*vaJzRp9ohwsGNYf368uI!/b/dMYAAAAAAAAA&amp;bo=nQAnAQAAAAADAJ4! " width="156.0"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI <span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry4Aa4+Extended FMDV 2A</span><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">-pChlamy_3<span></span></span></p>
  
<div class="highlight">
+
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
<h4>Note</h4>
+
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
+
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry10Aa4+Extended FMDV 2A<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in Top10<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2158bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
  
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/bQ8OOekpGDUmt.8vkQvJx0XUpn6PMh1VgaiF0dasSRg!/b/dAYBAAAAAAAA&amp;bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="308.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LjSeZIndHm9zkUE6gT36bEx3VnYrEwCbYS7Aq.zBlqw!/b/dMcAAAAAAAAA&amp;bo=7QA1AQAAAAADAPw! " width="236.8000030517578"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry11Aa4+ Extended FMDV 2A</span><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">-pChlamy_3<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry11Aa4+ Extended FMDV 2A<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in JM108<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2071bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0.upRVhjL7OQjfkLGru*.dUocUXc4fDAJpeQEXjZe8!/b/dMYAAAAAAAAA&amp;bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="308.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0MvndoK9mmRt.kJuTifErWbDLJSPJui*8L*GLlyeGl8!/b/dMYAAAAAAAAA&amp;bo=qAA1AQAAAAADALk! " width="167.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic">&nbsp;</span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cyt1-pChlamy_3<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cyt1<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in JM108<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">762bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Sy.SKFU5edTQSIF.h2uwVTZjSgxUj3zmtAOCAGbAaZo!/b/dMgAAAAAAAAA&amp;bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="279.20001220703125" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/xncHHhLWQz1aqSpaH4UR7R5bMZ4zFZ*PmV1nZxwVTQ8!/b/dAgBAAAAAAAA&amp;bo=sAAYAQAAAAADAIw! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic">&nbsp;</span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cyt2-pChlamy_3<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cyt1<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in JM108<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">801bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0mowbkc0M494OfDGRHIGtmKGQNkXPPWleN7nPIwSf0!/b/dMgAAAAAAAAA&amp;bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="280.0" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/irbPRy7*zARuesyKRBXZFk6ZWMGm44ZwwyjWYNaqYZA!/b/dAYBAAAAAAAA&amp;bo=4QAZAQAAAAADANw! " width="224.0"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td style="border:none;padding:0pt 0pt 0pt 0pt" width="54">      <p>&nbsp;</p>
 +
    </td>  </tr>      <tr height="0">      <td style="border:none" width="138"></td>      <td style="border:none" width="45"></td>      <td style="border:none" width="124"></td>      <td style="border:none" width="107"></td>      <td style="border:none" width="80"></td>      <td style="border:none" width="2"></td>      <td style="border:none" width="54"></td>    </tr>  </tbody>
 +
</table>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">EGFP-pChlamy_3<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">06-EGFP</span><span style="font-family:SimSun;font-size:11.0pt">(</span><span style="font-family:Calibri;font-size:11.0pt">TAA</span><span style="font-family:SimSun;font-size:11.0pt">)</span><span style="font-family:SimSun;font-size:11.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in Top10<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">732bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b260.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/kV2mqmj1q8v1ump215nFHpnjMUzwxqSu0DronaWKuIs!/b/dAQBAAAAAAAA&amp;bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="296.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/aRGJqrgiOwOJDeH5hDKr.6c7zKU8VMfcgFHbm35PZ6I!/b/dMcAAAAAAAAA&amp;bo=8AApAQAAAAADAP0! " width="239.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry4Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry4Aa4+Extended2A+Cyt2<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in Top10<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">4461bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"><span>&nbsp;</span><span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/uN*b2mkL9gaipMv8XpN.4wpt8raBNnOQuGwbKC.w*vE!/b/dMcAAAAAAAAA&amp;bo=qQD*AAAAAAADAHM! " width="168.8000030517578"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="236.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JI.J1CeIa*MhQu9aafo8T8eZeOr7zqxlK5q6W3NjbEA!/b/dMgAAAAAAAAA&amp;bo=mADtAAAAAAADAFA! " width="151.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"><span>&nbsp;</span><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and BamHI<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic"><span>&nbsp;</span></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry4Aa4+Extended2A+Cyt1-pChlamy3<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry4Aa4+Extended2A+Cyt1-pChlamy3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in JM108<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">4422bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pDvRNLD368siSdiKOdhck0A80upc24m2Aqj5qn.yyfY!/b/dMgAAAAAAAAA&amp;bo=qQD*AAAAAAADAHM! " width="168.8000030517578"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="280.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LqdjCnlIs0z8YJ*6kbyj5ACC8gGPT6IlK2OdWcxc4T0!/b/dMcAAAAAAAAA&amp;bo=rgAZAQAAAAADAJM! " width="173.60000610351562"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by BamHI and KpnI<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic">&nbsp;</span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td colspan="2" style="border:none;padding:0pt 0pt 0pt 0pt" width="63">      <p>&nbsp;</p>
 +
    </td>  </tr>      <tr height="0">      <td style="border:none" width="137"></td>      <td style="border:none" width="39"></td>      <td style="border:none" width="132"></td>      <td style="border:none" width="51"></td>      <td style="border:none" width="128"></td>      <td style="border:none" width="7"></td>      <td style="border:none" width="56"></td>    </tr>  </tbody>
 +
</table>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry10Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry10Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in DH5</span><span style="font-family:Calibri;font-size:11.0pt">α</span><span style="font-family:Calibri;font-size:11.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2946bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/H1*GSbsbhQbTR1eeNDiS8fA5AfLGIser.g5DKjHyGtU!/b/dMgAAAAAAAAA&amp;bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="264.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JfiiusuOJGnfyciEpeNFbNeC5oqcVaFFkIBoPRxw6x0!/b/dMYAAAAAAAAA&amp;bo=jQAJAQAAAAADAKA! " width="140.0"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic">&nbsp;</span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">&nbsp;</span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">C</span><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">ry</span><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">10Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry10Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in DH5</span><span style="font-family:Calibri;font-size:11.0pt">α</span><span style="font-family:Calibri;font-size:11.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2946bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/9p5OCN4X0ji10gEBq68TdCUN93aBGaDnJIBTc5E0f3M!/b/dMcAAAAAAAAA&amp;bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="273.6000061035156" src="http://b259.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/1zft.p5lOoX2yVoxccDyfQWcQjbGRb2SD*Zq97vSedo!/b/dAMBAAAAAAAA&amp;bo=kQASAQAAAAADAKc! " width="144.8000030517578"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic">&nbsp;</span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry10Aa4+Extended2A+Cyt1-pChlamy3 <span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry10Aa4+Extended2A+Cyt1-pChlamy3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in DH5</span><span style="font-family:Calibri;font-size:11.0pt">α</span><span style="font-family:Calibri;font-size:11.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2907bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/qchMjMQfSkqMMyaq5LhKw3ZuaGXoJRnrEvO0Slo0S1A!/b/dAYBAAAAAAAA&amp;bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="288.79998779296875" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Rme26s7JjvB5bA9BlIrNu00W2reMmKwHkx9yaus6ikU!/b/dMgAAAAAAAAA&amp;bo=zQAhAQAAAAADAMg! " width="204.8000030517578"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>    <td colspan="2" style="border:none;padding:0pt 0pt 0pt 0pt" width="60">      <p>&nbsp;</p>
 +
    </td>  </tr>      <tr height="0">      <td style="border:none" width="135"></td>      <td style="border:none" width="45"></td>      <td style="border:none" width="131"></td>      <td style="border:none" width="78"></td>      <td style="border:none" width="101"></td>      <td style="border:none" width="5"></td>      <td style="border:none" width="55"></td>    </tr>  </tbody>
 +
</table>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry11Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry11Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in Top10<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2859bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0LciDoc2Vues3GO6fYi7EnNqrsVS8mSF.qFDZziDpEA!/b/dMgAAAAAAAAA&amp;bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="258.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/hJNHsLw6HFbz9eR7eszEpW*U.RfWwyhYSlkMIPiWAbc!/b/dMYAAAAAAAAA&amp;bo=wwADAQAAAAADAOQ! " width="194.39999389648438"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by </span><span style="font-family:Calibri;font-size:11.0pt"> XbaI and PstI</span><span style="font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry11Aa4+Extended2A+Cyt1-pChlamy3<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry11Aa4+Extended2A+Cyt1-pChlamy3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in JM108<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2820bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/.39If2pAeG1h2OXRhp49InOl3WTN1asUm8AXJFwwqZs!/b/dAgBAAAAAAAA&amp;bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="264.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pZ5hAlXd5CMg3MJ.c2OE2MHz2ke4Ev3fW3jyPfdOUPU!/b/dMgAAAAAAAAA&amp;bo=vQAJAQAAAAADAJA! " width="188.0"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic">&nbsp;</span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
<p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry11Aa4+Extended2A+EGFP-pChlamy3<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry11Aa4+Extended2A+EGFP-pChlamy3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in JM108<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2790bp<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr>  <tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
 +
<table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0">
 +
  <tbody><tr>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/cI8hqU6TnQQhJWgxrwgmQv0Mwgv4T3KwluBqsv0K9KA!/b/dAYBAAAAAAAA&amp;bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="259.20001220703125" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/94oeXP5iFtJKrKNWD4RQCmRJXVM2sO.rQztD9R2O50w!/b/dMYAAAAAAAAA&amp;bo=qQAEAQAAAAADAIk! " width="168.8000030517578"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
    </td>    <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top">      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
      <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic">&nbsp;</span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p>
 +
      <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
    </td>  </tr></tbody>
 +
</table>
 +
<p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">&nbsp;<span></span></span></p>
 +
<p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:等线">&nbsp;</span></p>
 
</div>
 
</div>
 +
    </div>
 +
<br/>
 +
</div>
 +
  
 
</div>
 
</div>
  
 
</html>
 
</html>

Revision as of 03:35, 14 October 2016

Cry4Aa4+Extended FMDV 2A-pChlamy_3

Gene Name

Cry4Aa4+Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

3673bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cry4Aa4+Extended FMDV 2A-pChlamy_3

Gene Name

Cry10Aa4+Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

2158bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cry11Aa4+ Extended FMDV 2A-pChlamy_3

Gene Name

Cry11Aa4+ Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2071bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

Cyt1-pChlamy_3

Gene Name

Cyt1

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

762bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

 

Cyt2-pChlamy_3

Gene Name

Cyt1

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

801bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

 

 

 

EGFP-pChlamy_3

Gene Name

06-EGFPTAA

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

732bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

Cry4Aa4+Extended2A+Cyt2-pChlamy3

Gene Name

Cry4Aa4+Extended2A+Cyt2

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

4461bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and BamHI

 

Lane 2: Plasmid DNA

 

 

 

 

 

 

Cry4Aa4+Extended2A+Cyt1-pChlamy3

Gene Name

Cry4Aa4+Extended2A+Cyt1-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

4422bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by BamHI and KpnI

 

Lane 2: Plasmid DNA

 

 

 

Cry10Aa4+Extended2A+Cyt2-pChlamy3

 

Gene Name

Cry10Aa4+Extended2A+Cyt2-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

2946bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

 

 

Cry10Aa4+Extended2A+Cyt2-pChlamy3

Gene Name

Cry10Aa4+Extended2A+Cyt2-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

2946bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

Cry10Aa4+Extended2A+Cyt1-pChlamy3

Gene Name

Cry10Aa4+Extended2A+Cyt1-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

2907bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

Cry11Aa4+Extended2A+Cyt2-pChlamy3

Gene Name

Cry11Aa4+Extended2A+Cyt2-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

2859bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cry11Aa4+Extended2A+Cyt1-pChlamy3

Gene Name

Cry11Aa4+Extended2A+Cyt1-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2820bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

 

Cry11Aa4+Extended2A+EGFP-pChlamy3

Gene Name

Cry11Aa4+Extended2A+EGFP-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2790bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA