Difference between revisions of "Team:Sheffield/project/science/growthcurves"
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<h2>Introduction and Aims</h2> | <h2>Introduction and Aims</h2> | ||
| − | < | + | <p>Our proposed reporter systems required an E. coli strain that allows the manipulation of intracellular iron levels via |
| − | siderophore uptake. We chose to use the JC28 strain that has an entC knockout mutation meaning that it is unable | + | siderophore uptake. We chose to use the JC28 strain that has an entC knockout mutation, meaning that it is unable |
| − | to produce siderophores. In order to keep intracellular iron-levels of JC28 low while the strain still grows | + | to produce siderophores. In order to keep intracellular iron-levels of JC28 low while the strain still grows, we needed to test a range of media and varying iron concentrations. </p> |
| − | test a range of media and varying iron concentrations.</p> | + | |
<p>We carried out growth curve experiments to form an understanding of the growth model of both the W3110 (<button class="btn btn-lg btn-danger" data-toggle="popover" title="Science word" data-content="">Wild Type</button>) </p> | <p>We carried out growth curve experiments to form an understanding of the growth model of both the W3110 (<button class="btn btn-lg btn-danger" data-toggle="popover" title="Science word" data-content="">Wild Type</button>) </p> | ||
<button class="btn btn-lg btn-danger" data-toggle="popover" title="Popover title" data-content="And here's some amazing content. It's very engaging. Right?">Click to toggle popover</button>) and JC28 (mutant) E. coli strains. As part of this, we cultured the <button class="btn btn-lg btn-danger" data-toggle="popover" title="Science word" data-content="">Wild Type</button> and mutant strains and measured the growth of the cultures both indirectly, by measuring optical density (fig 1.), and directly, by counting the number of colony forming units (CFU) plated on agar plates (fig 2.).</p> | <button class="btn btn-lg btn-danger" data-toggle="popover" title="Popover title" data-content="And here's some amazing content. It's very engaging. Right?">Click to toggle popover</button>) and JC28 (mutant) E. coli strains. As part of this, we cultured the <button class="btn btn-lg btn-danger" data-toggle="popover" title="Science word" data-content="">Wild Type</button> and mutant strains and measured the growth of the cultures both indirectly, by measuring optical density (fig 1.), and directly, by counting the number of colony forming units (CFU) plated on agar plates (fig 2.).</p> | ||
Revision as of 11:14, 15 October 2016