Line 27: | Line 27: | ||
<h1>Chemicals</h1> | <h1>Chemicals</h1> | ||
− | < | + | <!-- Ethidium Bromide --> |
− | <h2> | + | <h2>Ethidium Bromide</h2> |
− | <p> | + | <p>Use: Staining DNA electrophoresis gels.</p> |
− | + | <p>Signal word Danger</p> | |
− | </p> | + | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> |
− | </ | + | <ul> |
− | < | + | <li>H302 Harmful if swallowed.</li> |
− | <h2> | + | <li>H330 Fatal if inhaled.</li> |
− | <p> | + | <li>H341 Suspected of causing genetic defects.</li> |
− | + | </ul> | |
− | </p> | + | <p style="text-align:center"><strong>Precautionary statement(s)</strong></p> |
− | </ | + | <ul> |
− | < | + | <li>P260 Do not breathe dust/fume/gas/mist/vapours/spray.</li> |
− | <h2> | + | <li>P281 Use personal protective equipment as required.</li> |
− | <p> | + | <li>P284 Wear respiratory protection.</li> |
− | + | <li>P310 Immediately call a POISON CENTER or doctor/ physician.</li> | |
− | </p> | + | </ul> |
− | </ | + | <!-- TBE --> |
− | </ | + | <h2>TBE Buffer pH 8.3</h2> |
− | </ | + | <p>Use: General buffer for DNA experiments.</p> |
+ | <p>Non-Hazardous</p> | ||
+ | <!-- Copper Sulphate --> | ||
+ | <h2>Copper Sulphate Pentahydrate:</h2> | ||
+ | <p>Use: Induction of copper promotors and Cu2+ chelation assays.</p> | ||
+ | <p>Signal word: Warning</p> | ||
+ | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H302 Harmful if swallowed.</li> | ||
+ | <li>H315 Causes skin irritation.</li> | ||
+ | <li>H319 Causes serious eye irritation.</li> | ||
+ | <li>H410 Very toxic to aquatic life with long lasting effects.</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P273 Avoid release to the environment.</li> | ||
+ | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.</li> | ||
+ | <li>P501 Dispose of contents/container to an approved waste disposal plant.</li> | ||
+ | </ul> | ||
+ | <!-- agarose --> | ||
+ | <h2>Agarose</h2> | ||
+ | <p>Use: DNA electrophoresis gel matrix</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <h2>L(+)-Arabinose</h2> | ||
+ | <p>Use: Induction of pBAD expression</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <!-- Bathocuproinedisulfonic Acid Disodium salt --> | ||
+ | <h2>Bathocuproinedisulfonic Acid Disodium Salt</h2> | ||
+ | <p>Use: Chromophore in Cu+/Cu2+ detection assay</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <!-- Ascorbate --> | ||
+ | <h2>L(+)-Sodium Ascorbate</h2> | ||
+ | <p>Use: Reducing agent in Cu+/Cu2+ detection assay</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <!-- Glutathione --> | ||
+ | <h2>L-Glutathione</h2> | ||
+ | <p>Use: Reducing agent in Cu+/Cu2+ detection assay.</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <!-- Chloramphenicol --> | ||
+ | <h2>Chloramphenicol</h2> | ||
+ | <p>Use: Antibiotic and positive selection marker for all plasmids except pBAD</p> | ||
+ | <p>Signal word: Danger</p> | ||
+ | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H350 May cause cancer</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P201 Obtain special instructions before use.</li> | ||
+ | <li>P308 + P313 If exposed or concerned: Get medical advice/attention.</li> | ||
+ | </ul> | ||
+ | <!-- Ampicillin --> | ||
+ | <h2>Ampicillin</h2> | ||
+ | <p>Use: Antibiotic and positive selection marker for pBAD.</p> | ||
+ | <p>Signal word: Danger</p> | ||
+ | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H315 Causes skin irritation</li> | ||
+ | <li>H317 May cause an allergic skin reaction</li> | ||
+ | <li>H319 Causes serious eye irritation</li> | ||
+ | <li>H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled</li> | ||
+ | <li>H335 May cause respiratory irritation</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P261 Avoid breathing dust/fume/gas/mist/vapours/spray</li> | ||
+ | <li>P280 Wear protective gloves</li> | ||
+ | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | ||
+ | <li>P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician</li> | ||
+ | </ul> | ||
+ | <!-- Tris --> | ||
+ | <h2>Tris pH 7.0 and 8.0</h2> | ||
+ | <p>Use: General buffer for protein and copper assays</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <!-- Glycerol --> | ||
+ | <h2>Glycerol</h2> | ||
+ | <p>Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <!-- DTT --> | ||
+ | <h2>DL-Dithiothreitol</h2> | ||
+ | <p>Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification</p> | ||
+ | <p>Signal word: Warning</p> | ||
+ | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H302 Harmful if swallowed</li> | ||
+ | <li>H315 Causes skin irritation</li> | ||
+ | <li>H319 Causes serious eye irritation</li> | ||
+ | <li>H335 May cause respiratory irritation</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P261 Avoid breathing dust</li> | ||
+ | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | ||
+ | </ul> | ||
+ | <!-- Imidiazole --> | ||
+ | <h2>Imidazole</h2> | ||
+ | <p>Use: Elution of His-tagged target proteins from nickel bead column during protein purification</p> | ||
+ | <p>Signal word: Danger</p> | ||
+ | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H302 Harmful if swallowed</li> | ||
+ | <li>H314 Causes severe skin burns and eye damage</li> | ||
+ | <li>H360D May damage the unborn child</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P201 Obtain special instructions before use</li> | ||
+ | <li>P280 Wear protective gloves/protective clothing/eye protection/face protection</li> | ||
+ | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | ||
+ | <li>P310 Immediately call a POISON CENTER or doctor/physician</li> | ||
+ | </ul> | ||
+ | <!-- Nickel affinity beads --> | ||
+ | <h2>Nickel Affinity Beads</h2> | ||
+ | <p>Use: Selection of His-tagged target proteins during protein purification</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <!-- Sodium Alginate --> | ||
+ | <h2>Sodium Alginate</h2> | ||
+ | <p>Use: Major component of bacterial delivery beads</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <!-- Crystal Violet --> | ||
+ | <h2>Crystal Violet</h2> | ||
+ | <p>Use: Marker for bead quality assay</p> | ||
+ | <p>Signal word: Danger</p> | ||
+ | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H302 Harmful if swallowed</li> | ||
+ | <li>H318 Causes serious eye damage</li> | ||
+ | <li>H350 May cause cancer</li> | ||
+ | <li>H410 Very toxic to aquatic life with long lasting effects</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P201 Obtain special instructions before use</li> | ||
+ | <li>P273 Avoid release to the environment</li> | ||
+ | <li>P280 Wear protective gloves/ eye protection/ face protection</li> | ||
+ | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | ||
+ | <li>P308 + P313 If exposed or concerned: Get medical advice/attention</li> | ||
+ | <li>P501 Dispose of contents/container to an approved waste disposal plant</li> | ||
+ | </ul> | ||
+ | <!-- Lysogeny Broth --> | ||
+ | <h2>Lysogeny Broth</h2> | ||
+ | <p>Use: Media for general bacterial growth</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <!-- Phosphate buffered saline --> | ||
+ | <h2>Phosphate Buffered Saline pH 7.4</h2> | ||
+ | <p>Use: Dilution buffer for flow cytometry experiments</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <!-- SDS --> | ||
+ | <h2>Sodium Dodecyl Sulphate</h2> | ||
+ | <p>Use: Detergent in protein identification gels</p> | ||
+ | <p>Signal word: Danger</p> | ||
+ | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H228 Flammable solid</li> | ||
+ | <li>H302 + H332 Harmful if swallowed or if inhaled</li> | ||
+ | <li>H315 Causes skin irritation</li> | ||
+ | <li>H318 Causes serious eye damage</li> | ||
+ | <li>H335 May cause respiratory irritation</li> | ||
+ | <li>H412 Harmful to aquatic life with long lasting effects</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking</li> | ||
+ | <li>P261 Avoid breathing dust/fume/gas/mist/vapours/spray</li> | ||
+ | <li>P280 Wear eye protection/face protection</li> | ||
+ | <li>P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth</li> | ||
+ | <li>P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor</li> | ||
+ | <li>P370 + P378 In case of fire: Use dry powder or dry sand to extinguish</li> | ||
+ | </ul> | ||
+ | <!-- Ethanol --> | ||
+ | <h2>Ethanol 100%</h2> | ||
+ | <p>Use: Solvent for chloramphenicol</p> | ||
+ | <p>Signal word: Danger</p> | ||
+ | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H225 Highly flammable liquid and vapour</li> | ||
+ | <li>H319 Causes serious eye irritation</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking</li> | ||
+ | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | ||
+ | <li>P370 + P378 In case of fire: Use dry powder or dry sand to extinguish</li> | ||
+ | <li>P403 + P235 Store in a well-ventilated place. Keep cool</li> | ||
+ | </ul> | ||
+ | <!-- TFB I--> | ||
+ | <h2>Transfection Buffer I (Manganese[II] Chloride)</h2> | ||
+ | <p>Use: First soaking buffer in creating competent E. coli cells</p> | ||
+ | <p>Signal word: Danger</p> | ||
+ | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H302 Harmful if swallowed</li> | ||
+ | <li>H318 Causes serious eye damage</li> | ||
+ | <li>H373 May cause damage to organs (Brain) through prolonged or repeated exposure if inhaled</li> | ||
+ | <li>H411 Toxic to aquatic life with long lasting effects</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P273 Avoid release to the environment</li> | ||
+ | <li>P280 Wear eye protection/ face protection</li> | ||
+ | <li>P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Rinse mouth</li> | ||
+ | <li>P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician</li> | ||
+ | <li>P391 Collect spillage</li> | ||
+ | <li>P501 Dispose of contents/container to an approved waste disposal plant</li> | ||
+ | </ul> | ||
+ | <!-- TFB II --> | ||
+ | <h2>Transfection Buffer II</h2> | ||
+ | <p>Use: Second soaking buffer in creating competent E. coli cells</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <h2>Chitosan</h2> | ||
+ | <p>Use: Coating for bacterial delivery beads</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <h2>InstantBlue</h2> | ||
+ | <p>Use: Protein stain for protein identification gels</p> | ||
+ | <p>Signal word: Warning</p> | ||
+ | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H290 May be corrosive to metals</li> | ||
+ | <li>H315 Causes skin irritation</li> | ||
+ | <li>H319 Causes serious eye irritation</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | ||
+ | </ul> | ||
+ | <!-- Purple Loding Dye --> | ||
+ | <h2>NEB Purple Loading Dye</h2> | ||
+ | <p>Use: Stabilizer and marker for DNA gel electrophoresis</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <!-- Protease Inhibitors --> | ||
+ | <h2>Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2> | ||
+ | <p>Use: Prevention of protein cleavage during protein purification</p> | ||
+ | <p>Signal word Danger</p> | ||
+ | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H314 Causes severe skin burns and eye damage Skin corrosion/irritation</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P260 Do not breathe dust/fume/gas/mist/vapours/spray</li> | ||
+ | <li>P280 Wear protective gloves/protective clothing/eye protection/face protection</li> | ||
+ | <li>P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting</li> | ||
+ | <li>P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower</li> | ||
+ | <li>P304 + P340 + P310 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/physician</li> | ||
+ | <li>P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician</li> | ||
+ | </ul> | ||
+ | <!-- HCL --> | ||
+ | <h2>Hydrochloric Acid 1M</h2> | ||
+ | <p>Use: Simulation of stomach acid conditions in bead experiments</p> | ||
+ | <p>Signal word: Warning</p> | ||
+ | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H290 May be corrosive to metals</li> | ||
+ | </ul> | ||
+ | <!-- NaOH --> | ||
+ | <h2>Sodium Hydroxide</h2> | ||
+ | <p>Use: Simulation of duodenum conditions in bead experiments</p> | ||
+ | <p>Signal word: Danger</p> | ||
+ | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H290 May be corrosive to metals</li> | ||
+ | <li>H314 Causes severe skin burns and eye damage</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P260 Do not breathe dust/fume/gas/mist/vapours/spray</li> | ||
+ | <li>P280 Wear protective gloves/protective clothing/eye protection/face protection</li> | ||
+ | <li>P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower</li> | ||
+ | <li>P304 + P340 + P310 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor</li> | ||
+ | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | ||
+ | </ul> | ||
+ | <!-- Monobasic Sodium Phopshate --> | ||
+ | <h2>Monobasic Sodium Phosphate</h2> | ||
+ | <p>Use: Simulation of duodenum conditions in bead experiments</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | <!-- Glacial Acetic ACid --> | ||
+ | <h2>Glacial Acetic Acid</h2> | ||
+ | <p>Use: Bead experiments</p> | ||
+ | <p>Signal word: Danger</p> | ||
+ | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H226 Flammable liquid and vapour</li> | ||
+ | <li>H314 Causes severe skin burns and eye damage</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking</li> | ||
+ | <li>P260 Do not breathe dust/fume/gas/mist/vapours/spray</li> | ||
+ | <li>P280 Wear protective gloves/protective clothing/eye protection/face protection</li> | ||
+ | <li>P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower</li> | ||
+ | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | ||
+ | <li>P370 + P378 In case of fire: Use dry powder or dry sand to extinguish</li> | ||
+ | </ul> | ||
+ | <!-- CaCl --> | ||
+ | <h2>Calcium Chloride</h2> | ||
+ | <p>Use: Forming beads from alginate. Ca2+ causes alginate phase change</p> | ||
+ | <p>Signal word Warning</p> | ||
+ | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H319 Causes serious eye irritation</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | ||
+ | </ul> | ||
</body> | </body> | ||
</html> | </html> | ||
{{:Team:Oxford/footer}} | {{:Team:Oxford/footer}} |
Revision as of 20:37, 15 October 2016
Chemicals
Ethidium Bromide
Use: Staining DNA electrophoresis gels.
Signal word Danger
Hazard statement(s)
- H302 Harmful if swallowed.
- H330 Fatal if inhaled.
- H341 Suspected of causing genetic defects.
Precautionary statement(s)
- P260 Do not breathe dust/fume/gas/mist/vapours/spray.
- P281 Use personal protective equipment as required.
- P284 Wear respiratory protection.
- P310 Immediately call a POISON CENTER or doctor/ physician.
TBE Buffer pH 8.3
Use: General buffer for DNA experiments.
Non-Hazardous
Copper Sulphate Pentahydrate:
Use: Induction of copper promotors and Cu2+ chelation assays.
Signal word: Warning
Hazard statement(s)
- H302 Harmful if swallowed.
- H315 Causes skin irritation.
- H319 Causes serious eye irritation.
- H410 Very toxic to aquatic life with long lasting effects.
Precautionary statement(s)
- P273 Avoid release to the environment.
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P501 Dispose of contents/container to an approved waste disposal plant.
Agarose
Use: DNA electrophoresis gel matrix
Non-Hazardous
L(+)-Arabinose
Use: Induction of pBAD expression
Non-Hazardous
Bathocuproinedisulfonic Acid Disodium Salt
Use: Chromophore in Cu+/Cu2+ detection assay
Non-Hazardous
L(+)-Sodium Ascorbate
Use: Reducing agent in Cu+/Cu2+ detection assay
Non-Hazardous
L-Glutathione
Use: Reducing agent in Cu+/Cu2+ detection assay.
Non-Hazardous
Chloramphenicol
Use: Antibiotic and positive selection marker for all plasmids except pBAD
Signal word: Danger
Hazard statement(s)
- H350 May cause cancer
Precautionary statement(s)
- P201 Obtain special instructions before use.
- P308 + P313 If exposed or concerned: Get medical advice/attention.
Ampicillin
Use: Antibiotic and positive selection marker for pBAD.
Signal word: Danger
Hazard statement(s)
- H315 Causes skin irritation
- H317 May cause an allergic skin reaction
- H319 Causes serious eye irritation
- H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled
- H335 May cause respiratory irritation
Precautionary statement(s)
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician
Tris pH 7.0 and 8.0
Use: General buffer for protein and copper assays
Non-Hazardous
Glycerol
Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer
Non-Hazardous
DL-Dithiothreitol
Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification
Signal word: Warning
Hazard statement(s)
- H302 Harmful if swallowed
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statement(s)
- P261 Avoid breathing dust
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Imidazole
Use: Elution of His-tagged target proteins from nickel bead column during protein purification
Signal word: Danger
Hazard statement(s)
- H302 Harmful if swallowed
- H314 Causes severe skin burns and eye damage
- H360D May damage the unborn child
Precautionary statement(s)
- P201 Obtain special instructions before use
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P310 Immediately call a POISON CENTER or doctor/physician
Nickel Affinity Beads
Use: Selection of His-tagged target proteins during protein purification
Non-Hazardous
Sodium Alginate
Use: Major component of bacterial delivery beads
Non-Hazardous
Crystal Violet
Use: Marker for bead quality assay
Signal word: Danger
Hazard statement(s)
- H302 Harmful if swallowed
- H318 Causes serious eye damage
- H350 May cause cancer
- H410 Very toxic to aquatic life with long lasting effects
Precautionary statement(s)
- P201 Obtain special instructions before use
- P273 Avoid release to the environment
- P280 Wear protective gloves/ eye protection/ face protection
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P308 + P313 If exposed or concerned: Get medical advice/attention
- P501 Dispose of contents/container to an approved waste disposal plant
Lysogeny Broth
Use: Media for general bacterial growth
Non-Hazardous
Phosphate Buffered Saline pH 7.4
Use: Dilution buffer for flow cytometry experiments
Non-Hazardous
Sodium Dodecyl Sulphate
Use: Detergent in protein identification gels
Signal word: Danger
Hazard statement(s)
- H228 Flammable solid
- H302 + H332 Harmful if swallowed or if inhaled
- H315 Causes skin irritation
- H318 Causes serious eye damage
- H335 May cause respiratory irritation
- H412 Harmful to aquatic life with long lasting effects
Precautionary statement(s)
- P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P280 Wear eye protection/face protection
- P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth
- P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor
- P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
Ethanol 100%
Use: Solvent for chloramphenicol
Signal word: Danger
Hazard statement(s)
- H225 Highly flammable liquid and vapour
- H319 Causes serious eye irritation
Precautionary statement(s)
- P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
- P403 + P235 Store in a well-ventilated place. Keep cool
Transfection Buffer I (Manganese[II] Chloride)
Use: First soaking buffer in creating competent E. coli cells
Signal word: Danger
Hazard statement(s)
- H302 Harmful if swallowed
- H318 Causes serious eye damage
- H373 May cause damage to organs (Brain) through prolonged or repeated exposure if inhaled
- H411 Toxic to aquatic life with long lasting effects
Precautionary statement(s)
- P273 Avoid release to the environment
- P280 Wear eye protection/ face protection
- P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Rinse mouth
- P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician
- P391 Collect spillage
- P501 Dispose of contents/container to an approved waste disposal plant
Transfection Buffer II
Use: Second soaking buffer in creating competent E. coli cells
Non-Hazardous
Chitosan
Use: Coating for bacterial delivery beads
Non-Hazardous
InstantBlue
Use: Protein stain for protein identification gels
Signal word: Warning
Hazard statement(s)
- H290 May be corrosive to metals
- H315 Causes skin irritation
- H319 Causes serious eye irritation
Precautionary statement(s)
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
NEB Purple Loading Dye
Use: Stabilizer and marker for DNA gel electrophoresis
Non-Hazardous
Roche cOmplete EDTA-free Protease Inhibitor Cocktail
Use: Prevention of protein cleavage during protein purification
Signal word Danger
Hazard statement(s)
- H314 Causes severe skin burns and eye damage Skin corrosion/irritation
Precautionary statement(s)
- P260 Do not breathe dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
- P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower
- P304 + P340 + P310 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/physician
- P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician
Hydrochloric Acid 1M
Use: Simulation of stomach acid conditions in bead experiments
Signal word: Warning
Hazard statement(s)
- H290 May be corrosive to metals
Sodium Hydroxide
Use: Simulation of duodenum conditions in bead experiments
Signal word: Danger
Hazard statement(s)
- H290 May be corrosive to metals
- H314 Causes severe skin burns and eye damage
Precautionary statement(s)
- P260 Do not breathe dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
- P304 + P340 + P310 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Monobasic Sodium Phosphate
Use: Simulation of duodenum conditions in bead experiments
Non-Hazardous
Glacial Acetic Acid
Use: Bead experiments
Signal word: Danger
Hazard statement(s)
- H226 Flammable liquid and vapour
- H314 Causes severe skin burns and eye damage
Precautionary statement(s)
- P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
- P260 Do not breathe dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
Calcium Chloride
Use: Forming beads from alginate. Ca2+ causes alginate phase change
Signal word Warning
Hazard statement(s)
- H319 Causes serious eye irritation
Precautionary statement(s)
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing