Difference between revisions of "Team:Cornell NY/Proof"
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Bovine mastitis in dairy cattle is the most costly disease that plagues the agricultural industry worldwide, and costing the United States around $2 billion annually [1]. Traditional treatments usually involve the use of antibiotics. However, the use of antibiotics has led to increasing concerns of resistance as well as negative implications on human health. Furthermore, the development and synthesis of new antibiotics is slow and may not necessarily have the desired effects. | Bovine mastitis in dairy cattle is the most costly disease that plagues the agricultural industry worldwide, and costing the United States around $2 billion annually [1]. Traditional treatments usually involve the use of antibiotics. However, the use of antibiotics has led to increasing concerns of resistance as well as negative implications on human health. Furthermore, the development and synthesis of new antibiotics is slow and may not necessarily have the desired effects. | ||
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| − | <img class="img-circle" src="https://static.igem.org/mediawiki/2016/6/6d/T--Cornell_NY--Aureocin.png" style="width: | + | <img class="img-circle" src="https://static.igem.org/mediawiki/2016/6/6d/T--Cornell_NY--Aureocin.png" style="width:20%;height:20%;"> |
<p class="lead mb40">Bacteriocins are natural antimicrobial peptides secreted by bacteria to gain competitive advantage in their natural ecosystems. Functionally, bacteriocins kill target cells via different mechanisms, including pore formation, inhibition of translation, inhibition of transcription, and inhibition of cell wall synthesis. The use of bacteriocins as antimicrobial drugs has three significant advantages. First, bacteriocins are known not to cause harm to humans. One such group of bacteriocins, the Nisin family, is approved by the Food and Drug Administration as a food preservative [2]. Second, bacteriocins have a remarkably fast evolution rate [3]. Finally, bacteriocins are known to be very specific to certain species within the same genus, although some are known to have broad spectrum activity as well. This diversity among bacteriocins, along with their safety for human consumption, makes them a promising alternative to traditional antibiotics. </p> | <p class="lead mb40">Bacteriocins are natural antimicrobial peptides secreted by bacteria to gain competitive advantage in their natural ecosystems. Functionally, bacteriocins kill target cells via different mechanisms, including pore formation, inhibition of translation, inhibition of transcription, and inhibition of cell wall synthesis. The use of bacteriocins as antimicrobial drugs has three significant advantages. First, bacteriocins are known not to cause harm to humans. One such group of bacteriocins, the Nisin family, is approved by the Food and Drug Administration as a food preservative [2]. Second, bacteriocins have a remarkably fast evolution rate [3]. Finally, bacteriocins are known to be very specific to certain species within the same genus, although some are known to have broad spectrum activity as well. This diversity among bacteriocins, along with their safety for human consumption, makes them a promising alternative to traditional antibiotics. </p> | ||
Revision as of 23:29, 15 October 2016
Wetlab
Overview
Bovine mastitis in dairy cattle is the most costly disease that plagues the agricultural industry worldwide, and costing the United States around $2 billion annually [1]. Traditional treatments usually involve the use of antibiotics. However, the use of antibiotics has led to increasing concerns of resistance as well as negative implications on human health. Furthermore, the development and synthesis of new antibiotics is slow and may not necessarily have the desired effects.
Bacteriocins are natural antimicrobial peptides secreted by bacteria to gain competitive advantage in their natural ecosystems. Functionally, bacteriocins kill target cells via different mechanisms, including pore formation, inhibition of translation, inhibition of transcription, and inhibition of cell wall synthesis. The use of bacteriocins as antimicrobial drugs has three significant advantages. First, bacteriocins are known not to cause harm to humans. One such group of bacteriocins, the Nisin family, is approved by the Food and Drug Administration as a food preservative [2]. Second, bacteriocins have a remarkably fast evolution rate [3]. Finally, bacteriocins are known to be very specific to certain species within the same genus, although some are known to have broad spectrum activity as well. This diversity among bacteriocins, along with their safety for human consumption, makes them a promising alternative to traditional antibiotics.
The pathogens of bovine mastitis can be divided broadly into Gram positive and Gram negative species, as well as contagious or environmental species. Our choice of bacteriocins is such that we can target the pathogens from all categories. Some potent bacteriocins have incredibly complex post-translational modifications, but we have also chosen ones that don’t require any modifications. The genes will be cloned into the standard pSB1C3 vector, and the peptides heterologously expressed by inducing the expression of T7 RNA Polymerase in Escherichia coli BL21(DE3). The efficacy of these recombinant proteins will be tested via zone of inhibition assays and growth curve assays.
