Difference between revisions of "Team:Northwestern/09 10"

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     <h1>Saturday, September 10<sup>th</sup></h3>
 
     <h1>Saturday, September 10<sup>th</sup></h3>
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<h2>Tasks:</h2>
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        <p>Jordan &amp; Tyler</p>
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        <ul>
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          <li>Ran a Gibson on Cas9-Lrz-SS and TorA (with PCR-added homology)</li>
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          <li>Made 5 mL overnight cultures of:</li>
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          <ul>
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            <li>10 colonies from Cas9-DsbA Gibson assembly product with PCR-added homology to insert</li>
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            <li>2 colonies from Cas9-DsbA Gibson assembly product with original DsbA gBlock</li>
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          </ul>
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          <li>Gibson reaction of TorA-Cas9:</li>
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          <ul>
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            <li>3.3µL water</li>
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            <li>1.16 µL backbone</li>
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            <li>0.54µL insert</li>
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            <li>5µL Gibson Mix</li>
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          </ul>
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          <li>Miniprep Results:</li>
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            <div class="col-sm-8"><img src="https://static.igem.org/mediawiki/2016/e/e9/T--Northwestern--09_10_1.png" width="1074" height="730" alt=""/></div>
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          </div>
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        </ul>
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      </div>
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    </div>
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Revision as of 03:03, 16 October 2016

Notebook

Saturday, September 10th

Tasks:

Jordan & Tyler

  • Ran a Gibson on Cas9-Lrz-SS and TorA (with PCR-added homology)
  • Made 5 mL overnight cultures of:
    • 10 colonies from Cas9-DsbA Gibson assembly product with PCR-added homology to insert
    • 2 colonies from Cas9-DsbA Gibson assembly product with original DsbA gBlock
  • Gibson reaction of TorA-Cas9:
    • 3.3µL water
    • 1.16 µL backbone
    • 0.54µL insert
    • 5µL Gibson Mix
  • Miniprep Results: