Difference between revisions of "Team:OUC-China"
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<body> | <body> | ||
| − | <div class="container"> | + | <div class="container" style="width:100%"> |
<nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation"> | <nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation"> | ||
| + | <div class="container"> | ||
| + | <nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation"> | ||
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<a class="navbar-brand oucBrand" href="#"><img src="https://static.igem.org/mediawiki/2016/f/fc/T--OUC-China--nav-logo-1.fw.png" alt="Brand"></a> | <a class="navbar-brand oucBrand" href="#"><img src="https://static.igem.org/mediawiki/2016/f/fc/T--OUC-China--nav-logo-1.fw.png" alt="Brand"></a> | ||
</div> | </div> | ||
| + | <div class="collapse navbar-collapse" id="example-navbar-collapse"> | ||
| + | <ul class="nav navbar-nav" id="oucNav"> | ||
| + | <li class="active"><a href="https://2016.igem.org/Team:OUC-China">Home</a></li> | ||
| + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a> | ||
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| + | <li><a ref="https://2016.igem.org/Team:OUC-China/Project">Introduction</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Results">Results</a></li> | ||
| + | </ul> | ||
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| + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a> | ||
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| + | <li><a href="https://2016.igem.org/Team:OUC-China/Parts">Parts</a></li> | ||
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| + | <li><a href="https://2016.igem.org/Team:OUC-China/Composite_Part">Composite parts</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Part_Collection">Part collection</a></li> | ||
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| + | <li><a href="https://2016.igem.org/Team:OUC-China/Communicating_&_improving">Communicating & improving</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Investigating_&_promoting">Investigating & promoting</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li> | ||
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| + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<span class="caret"></span></a> | ||
| + | <ul class="dropdown-menu"> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<span class="caret"></span></a> | ||
| + | <ul class="dropdown-menu"> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Software">Software</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Attributions">Attributions</a></li> | ||
| + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Safety<span class="caret"></span></a> | ||
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| + | <li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Protocol">Protocol</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Safety">Safety</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | </div> | ||
| + | </nav> | ||
<div class="collapse navbar-collapse" id="example-navbar-collapse"> | <div class="collapse navbar-collapse" id="example-navbar-collapse"> | ||
<ul class="nav navbar-nav" id="oucNav"> | <ul class="nav navbar-nav" id="oucNav"> | ||
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<div class="clearfix"></div> | <div class="clearfix"></div> | ||
<div class="text-center"> | <div class="text-center"> | ||
| − | <p>As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, we can decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.</p> | + | <p class="text-center">As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, we can decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.</p> |
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<div class="col-md-5"> | <div class="col-md-5"> | ||
| − | <h3> | + | <h3>Abort：</h3> |
<br> | <br> | ||
<p>Thanks to:<img src="https://static.igem.org/mediawiki/2016/5/57/T--OUC-China--foot1.jpg"alt="Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences"><img src="https://static.igem.org/mediawiki/2016/f/f0/T--OUC-China--foot2.jpg"alt="Biolabs"></p> | <p>Thanks to:<img src="https://static.igem.org/mediawiki/2016/5/57/T--OUC-China--foot1.jpg"alt="Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences"><img src="https://static.igem.org/mediawiki/2016/f/f0/T--OUC-China--foot2.jpg"alt="Biolabs"></p> | ||
Revision as of 03:59, 16 October 2016
ABSTRACT
As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, we can decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.
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