[2016-Attribution]

We are proud to say that all the ideas we worked on this year were proposed by undergraduate members of iGEM 2016 team Tokyo_Tech. We also want to thank all those advisors and instructors who helped us discuss these ideas and guided us through doing the experiments. The whole experimental data produced by our team comes from experiments all carried out by undergraduates.

The project "Snow White" was proposed by Ayako Tamaki.

[2016-member]

Performing all cloning (transformation, culture cells, mini prep)
  -Hiro Kondo

Performing parts construction (restriction digest, ligation)
  -Kazuki Fujisawa, Yuya Netsu, Hiro Kondo, Koji Nishikawa, Yoshio Takata

Performing Toxin assay
  -Kentaro Akiyama, Hiro Kondo

Performing AHL assay
  -Hazuki Hasegawa, Miki Nishimori

Performing AmiE assay
  -Nae Fu

Performing Pheat assay
  -Nae Fu

Performing Pcold assay
  -Nae Fu

Modeling
  -Shunya Akiyama, Ryuta Sasaki, Narumi Ogawa, Alex Llacsahuanga,

Wiki and slide design
  -Ayako Tamaki, Ryuta Sasaki, Mizuki Tabata, Maoko Takenoshita, Jiawan Sun, Alex Llacsahuanga, Guo Xin, Hajime Fujita with help from other team members

Human Practices
  -Hazuki Hasegawa, Nae Fu, Mako Kono, Nakahara Kengo, Nozomi Takeue with help from other team members

[2016-Contribution]

• General Support

  Prof. Nakashima and Prof. Hayashi let us use their labs for all of our experiments and gave us advice on experimental skills.

  Prof. Tagawa gave us an advice on our project and helped us set up a forum.

  Prof. Yamamura gave us advice on modeling.

  Promega and Leave a Nest gave us several restriction enzymes used in our experiment at no charge.

  We want to thank every advisers and instructors who helped us with discussing these ideas and guided us in the experiments.

• Instrumental Analysis Support

  Biomaterial Analysis Center, Technical Department, checked the sequence of the plasmids which we asked.

• Gene Synthesis

  The team would like to thank MBL and IDT.

• Human Practice Support

  Ochanomizu University Junior High School, Tokyo Metropolitan Nishi High School, Kichijo Girls’ School, Tokyo Gakugei University Senior High School, Kanagawa Prefectural Atsugi High School, and Koshigayakita High School accepted our School-visits.

  Tastuya Ogawa, who is the science communicator, the professional of communicating science to public, taught us the important lessons of communicating science or studies to public.

  Prof. Tsuge, Prof. Kakuta, and Jiro Nudeshima, who is working on The Tokyo Foundation, gave us lectures.

  Prof. Nakasaki gave us great and helpful advice.

• Presentation Adviser

  We would like to thank all the people who came to check our presentations and gave us useful feedbacks:

  Professor:

  Yasunori Aizawa, Takeshi Hata, Takashi Hirasawa, Junji Hirota, Hiroshi Iwasaki, Toshiaki Kamachi, Yasunori Tanji, Shinji Masuda, Yuki Yamaguchi