Chemicals

Ethidium Bromide

Use: Staining DNA electrophoresis gels.

Signal word Danger

Hazard statement(s)

• H302 Harmful if swallowed.
• H330 Fatal if inhaled.
• H341 Suspected of causing genetic defects.

Precautionary statement(s)

• P260 Do not breathe dust/fume/gas/mist/vapours/spray.
• P281 Use personal protective equipment as required.
• P284 Wear respiratory protection.
• P310 Immediately call a POISON CENTER or doctor/ physician.

TBE Buffer pH 8.3

Use: General buffer for DNA experiments.Made of three chemicals: Tris,borate and EDTA.

Non-Hazardous

Copper Sulphate Pentahydrate:

Use: Induction of copper promoters and Cu2+ chelation assays.

Signal word: Warning

Hazard statement(s)

• H302 Harmful if swallowed.
• H315 Causes skin irritation.
• H319 Causes serious eye irritation.
• H410 Very toxic to aquatic life with long lasting effects.

Precautionary statement(s)

• P273 Avoid release to the environment.
• P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.Remove contact lenses, if present and easy to do. Continue rinsing.
• P501 Dispose of contents/container to an approved waste disposal plant.

Agarose

Use: DNA electrophoresis gel matrix

Non-Hazardous

L(+)-Arabinose

Use: Induction of pBAD expression

Non-Hazardous

Bathocuproinedisulfonic Acid Disodium Salt

Use: Chromophore in Cu+/Cu2+ detection assay

Non-Hazardous

L(+)-Sodium Ascorbate

Use: Reducing agent in Cu+/Cu2+ detection assay

Non-Hazardous

L-Glutathione

Use: Reducing agent in Cu+/Cu2+ detection assay.

Non-Hazardous

Chloramphenicol

Use: Antibiotic and positive selection marker for all plasmids except pBAD

Signal word: Danger

Hazard statement(s)

• H350 May cause cancer

Precautionary statement(s)

• P201 Obtain special instructions before use.
• P308 + P313 If exposed or concerned: Get medical advice/attention.

Ampicillin

Use: Antibiotic and positive selection marker for pBAD.

Signal word: Danger

Hazard statement(s)

• H315 Causes skin irritation
• H317 May cause an allergic skin reaction
• H319 Causes serious eye irritation
• H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled
• H335 May cause respiratory irritation

Precautionary statement(s)

• P261 Avoid breathing dust/fume/gas/mist/vapours/spray
• P280 Wear protective gloves
• P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
• P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician

Tris pH 7.0 and 8.0

Use: General buffer for protein and copper assays

Non-Hazardous

Glycerol

Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer

Non-Hazardous

DL-Dithiothreitol

Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification

Signal word: Warning

Hazard statement(s)

• H302 Harmful if swallowed
• H315 Causes skin irritation
• H319 Causes serious eye irritation
• H335 May cause respiratory irritation

Precautionary statement(s)

• P261 Avoid breathing dust
• P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Imidazole

Use: Elution of His-tagged target proteins from nickel bead column during protein purification

Signal word: Danger

Hazard statement(s)

• H302 Harmful if swallowed
• H314 Causes severe skin burns and eye damage
• H360D May damage the unborn child

Precautionary statement(s)

• P201 Obtain special instructions before use
• P280 Wear protective gloves/protective clothing/eye protection/face protection
• P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
• P310 Immediately call a POISON CENTER or doctor/physician

Nickel Affinity Beads

Use: Selection of His-tagged target proteins during protein purification

Non-Hazardous

Sodium Alginate

Use: Major component of bacterial delivery beads

Non-Hazardous

Crystal Violet

Use: Marker for bead quality assay

Signal word: Danger

Hazard statement(s)

• H302 Harmful if swallowed
• H318 Causes serious eye damage
• H350 May cause cancer
• H410 Very toxic to aquatic life with long lasting effects

Precautionary statement(s)

• P201 Obtain special instructions before use
• P273 Avoid release to the environment
• P280 Wear protective gloves/ eye protection/ face protection
• P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
• P308 + P313 If exposed or concerned: Get medical advice/attention
• P501 Dispose of contents/container to an approved waste disposal plant

Lysogeny Broth

Use: Media for general bacterial growth

Non-Hazardous

Phosphate Buffered Saline pH 7.4

Use: Dilution buffer for flow cytometry experiments

Non-Hazardous

Sodium Dodecyl Sulphate

Use: Detergent in protein identification gels

Signal word: Danger

Hazard statement(s)

• H228 Flammable solid
• H302 + H332 Harmful if swallowed or if inhaled
• H315 Causes skin irritation
• H318 Causes serious eye damage
• H335 May cause respiratory irritation
• H412 Harmful to aquatic life with long lasting effects

Precautionary statement(s)

• P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
• P261 Avoid breathing dust/fume/gas/mist/vapours/spray
• P280 Wear eye protection/face protection
• P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth
• P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor
• P370 + P378 In case of fire: Use dry powder or dry sand to extinguish

Ethanol 100%

Use: solvent for chloramphenicol

Signal word: Danger

Hazard statement(s)

• H225 Highly flammable liquid and vapour
• H319 Causes serious eye irritation

Precautionary statement(s)

• P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
• P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
• P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
• P403 + P235 Store in a well-ventilated place. Keep cool

Transfection Buffer I (Manganese[II] Chloride)

Use: First soaking buffer in creating competent E. coli cells

Signal word: Danger

Hazard statement(s)

• H302 Harmful if swallowed
• H318 Causes serious eye damage
• H373 May cause damage to organs (Brain) through prolonged or repeated exposure if inhaled
• H411 Toxic to aquatic life with long lasting effects

Precautionary statement(s)

• P273 Avoid release to the environment
• P280 Wear eye protection/ face protection
• P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Rinse mouth
• P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician
• P391 Collect spillage
• P501 Dispose of contents/container to an approved waste disposal plant

Transfection Buffer II

Use: Second soaking buffer in creating competent E. coli cells

Non-Hazardous

Chitosan

Use: Coating for bacterial delivery beads

Non-Hazardous

InstantBlue

Use: Protein stain for protein identification gels

Signal word: Warning

Hazard statement(s)

• H290 May be corrosive to metals
• H315 Causes skin irritation
• H319 Causes serious eye irritation

Precautionary statement(s)

• P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

NEB Purple Loading Dye

Use: Stabilizer and marker for DNA gel electrophoresis

Non-Hazardous

Roche cOmplete EDTA-free Protease Inhibitor Cocktail

Use: Prevention of protein cleavage during protein purification

Signal word Danger

Hazard statement(s)

• H314 Causes severe skin burns and eye damage Skin corrosion/irritation

Precautionary statement(s)

• P260 Do not breathe dust/fume/gas/mist/vapours/spray
• P280 Wear protective gloves/protective clothing/eye protection/face protection
• P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
• P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower
• P304 + P340 + P310 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/physician
• P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician

Hydrochloric Acid 1M

Use: Simulation of stomach acid conditions in bead experiments

Signal word: Warning

Hazard statement(s)

• H290 May be corrosive to metals

Sodium Hydroxide

Use: Simulation of duodenum conditions in bead experiments

Signal word: Danger

Hazard statement(s)

• H290 May be corrosive to metals
• H314 Causes severe skin burns and eye damage

Precautionary statement(s)

• P260 Do not breathe dust/fume/gas/mist/vapours/spray
• P280 Wear protective gloves/protective clothing/eye protection/face protection
• P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
• P304 + P340 + P310 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor
• P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Monobasic Sodium Phosphate

Use: Simulation of duodenum conditions in bead experiments

Non-Hazardous

Glacial Acetic Acid

Use: Bead experiments

Signal word: Danger

Hazard statement(s)

• H226 Flammable liquid and vapour
• H314 Causes severe skin burns and eye damage

Precautionary statement(s)

• P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
• P260 Do not breathe dust/fume/gas/mist/vapours/spray
• P280 Wear protective gloves/protective clothing/eye protection/face protection
• P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
• P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
• P370 + P378 In case of fire: Use dry powder or dry sand to extinguish

Calcium Chloride

Use: Forming beads from alginate. Ca2+ causes alginate phase change

Signal word Warning

Hazard statement(s)

• H319 Causes serious eye irritation

Precautionary statement(s)

• P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing