Difference between revisions of "Team:UMaryland/Part Collection"

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Revision as of 23:21, 16 October 2016

</div> </div> Modeling

Parts Collection
BioBrick Devices Submitted to the Registry
Furthering collaboration and standardization of genetic parts

UMaryland iGEM submitted various basic and composite parts to the BioBrick Registry, which aims to increase standardization in synthetic biology by allowing genes to be added together easily. We synthesized the genes, put them inside standard BioBrick plasmids, and then characterized our parts.

View:

Basic Parts Composite Parts

Fructose Pathway (BBa_K2032001)

Contains genes MDH, HPS, and PHI. Methanol Dehydrogenase II converts methanol into formate. HPS and PHI turn it into metabolites that feed into the glycolysis pathway.

GroESL Composite (BBa_K2032005)

Contains promoter, rbs, and terminator along with GroESL coding region

Fructose with RFP (BBa_K2032006)

Fructose construct with RFP reporter

Formate with RFP (BBa_K2032007)

Formate construct with RFP reporter

Formate Pathway (BBa_K2032002)

Contains stop codons after parts FDH and FALDH! Converts methanol into carbon dioxide, generating energy in the form of NADPH in the process.

Codon optimized MDH2 with Lac/pL promoter (BBa_K2032003)

Codon optimized MDH2 with lac + pL promoter

GroESL (BBa_K2032004)

Chaperone complex