Difference between revisions of "Team:Oxford/Chemicals"
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<h2 id="Copper-Sulfate-Pentahydrate">Copper Sulphate Pentahydrate:</h2> | <h2 id="Copper-Sulfate-Pentahydrate">Copper Sulphate Pentahydrate:</h2> | ||
<p><u>Use:</u> Induction of copper promoters and Cu2+ chelation assays.</p> | <p><u>Use:</u> Induction of copper promoters and Cu2+ chelation assays.</p> | ||
| − | <p>u>Signal word:</u> Warning</p> | + | <p><u>Signal word:</u> Warning</p> |
<img src="https://static.igem.org/mediawiki/2016/b/b6/T--Oxford--CUSO4H2O.png" style="float: right" width="30%"/> | <img src="https://static.igem.org/mediawiki/2016/b/b6/T--Oxford--CUSO4H2O.png" style="float: right" width="30%"/> | ||
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<h2 id="Chloramphenicol">Chloramphenicol</h2> | <h2 id="Chloramphenicol">Chloramphenicol</h2> | ||
<p><u>Use:</u> Antibiotic and positive selection marker for all plasmids except pBAD</p> | <p><u>Use:</u> Antibiotic and positive selection marker for all plasmids except pBAD</p> | ||
| − | <p>u>Signal word:</u> Danger</p> | + | <p><u>Signal word:</u> Danger</p> |
<img src="https://static.igem.org/mediawiki/2016/c/cd/T--Oxford--Chloramphenicol.png" style="float: right" width="40%"/> | <img src="https://static.igem.org/mediawiki/2016/c/cd/T--Oxford--Chloramphenicol.png" style="float: right" width="40%"/> | ||
| Line 180: | Line 180: | ||
<h2 id="Ampicillin">Ampicillin</h2> | <h2 id="Ampicillin">Ampicillin</h2> | ||
<p><u>Use:</u> Antibiotic and positive selection marker for pBAD.</p> | <p><u>Use:</u> Antibiotic and positive selection marker for pBAD.</p> | ||
| − | <p>u>Signal word:</u> Danger</p> | + | <p><u>Signal word:</u> Danger</p> |
<img src="https://static.igem.org/mediawiki/2016/8/8f/T--Oxford--Ampicillin.png" style="float: right" width="40%"/> | <img src="https://static.igem.org/mediawiki/2016/8/8f/T--Oxford--Ampicillin.png" style="float: right" width="40%"/> | ||
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<h2 id="DL-Dithiothreitol">DL-Dithiothreitol</h2> | <h2 id="DL-Dithiothreitol">DL-Dithiothreitol</h2> | ||
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay and protein purification</p> | <p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay and protein purification</p> | ||
| − | <p>u>Signal word:</u> Warning</p> | + | <p><u>Signal word:</u> Warning</p> |
<img src="https://static.igem.org/mediawiki/2016/e/ee/T--Oxford--DTT.png" style="float: right" width="40%"/> | <img src="https://static.igem.org/mediawiki/2016/e/ee/T--Oxford--DTT.png" style="float: right" width="40%"/> | ||
| Line 241: | Line 241: | ||
<h2 id="Imidiazole">Imidazole</h2> | <h2 id="Imidiazole">Imidazole</h2> | ||
<p><u>Use:</u> Elution of His-tagged target proteins from nickel bead column during protein purification</p> | <p><u>Use:</u> Elution of His-tagged target proteins from nickel bead column during protein purification</p> | ||
| − | <p>u>Signal word:</u> Danger</p> | + | <p><u>Signal word:</u> Danger</p> |
<img src="https://static.igem.org/mediawiki/2016/0/01/T--Oxford--Imidiazole.png" style="float: right" width="30%"/> | <img src="https://static.igem.org/mediawiki/2016/0/01/T--Oxford--Imidiazole.png" style="float: right" width="30%"/> | ||
| Line 280: | Line 280: | ||
<h2 id="Crystal-Violet">Crystal Violet</h2> | <h2 id="Crystal-Violet">Crystal Violet</h2> | ||
<p><u>Use:</u> Marker for bead quality assay</p> | <p><u>Use:</u> Marker for bead quality assay</p> | ||
| − | <p>u>Signal word:</u> Danger</p> | + | <p><u>Signal word:</u> Danger</p> |
<img src="https://static.igem.org/mediawiki/2016/f/f6/T--Oxford--Crystal_Violet.png" style="float: right" width="30%"/> | <img src="https://static.igem.org/mediawiki/2016/f/f6/T--Oxford--Crystal_Violet.png" style="float: right" width="30%"/> | ||
| Line 348: | Line 348: | ||
<h2 id="Ethanol">Ethanol 100%</h2> | <h2 id="Ethanol">Ethanol 100%</h2> | ||
<p><u>Use:</u> solvent for chloramphenicol</p> | <p><u>Use:</u> solvent for chloramphenicol</p> | ||
| − | <p>u>Signal word:</u> Danger</p> | + | <p><u>Signal word:</u> Danger</p> |
<img src="https://static.igem.org/mediawiki/2016/b/b2/T--Oxford--Ethanol.png" style="float: right" width="60%"/> | <img src="https://static.igem.org/mediawiki/2016/b/b2/T--Oxford--Ethanol.png" style="float: right" width="60%"/> | ||
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<h2 id="Transfection-Buffer-I">Transfection Buffer I (Manganese[II] Chloride)</h2> | <h2 id="Transfection-Buffer-I">Transfection Buffer I (Manganese[II] Chloride)</h2> | ||
<p><u>Use:</u> First soaking buffer in creating competent E. coli cells</p> | <p><u>Use:</u> First soaking buffer in creating competent E. coli cells</p> | ||
| − | <p>u>Signal word:</u> Danger</p> | + | <p><u>Signal word:</u> Danger</p> |
<img src="https://static.igem.org/mediawiki/2016/f/fa/T--Oxford--MnCl.png" style="float: right"/> | <img src="https://static.igem.org/mediawiki/2016/f/fa/T--Oxford--MnCl.png" style="float: right"/> | ||
| Line 408: | Line 408: | ||
<h2 id="Instant-Blue">InstantBlue</h2> | <h2 id="Instant-Blue">InstantBlue</h2> | ||
<p><u>Use:</u> Protein stain for protein identification gels</p> | <p><u>Use:</u> Protein stain for protein identification gels</p> | ||
| − | <p>u>Signal word:</u> Warning</p> | + | <p><u>Signal word:</u> Warning</p> |
<img src="https://static.igem.org/mediawiki/2016/9/9c/T--Oxford--InstantBlue.png" style="float: right" width="50%"/> | <img src="https://static.igem.org/mediawiki/2016/9/9c/T--Oxford--InstantBlue.png" style="float: right" width="50%"/> | ||
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<h2 id="Protease-Inhibitors">Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2> | <h2 id="Protease-Inhibitors">Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2> | ||
<p><u>Use:</u> Prevention of protein cleavage during protein purification</p> | <p><u>Use:</u> Prevention of protein cleavage during protein purification</p> | ||
| − | <p>u>Signal word:</u> Danger</p> | + | <p><u>Signal word:</u> Danger</p> |
<img src="https://static.igem.org/mediawiki/2016/6/68/T--Oxford--protease_inhibitors.jpg" style="float: right"/> | <img src="https://static.igem.org/mediawiki/2016/6/68/T--Oxford--protease_inhibitors.jpg" style="float: right"/> | ||
| Line 457: | Line 457: | ||
<h2 id="Hydrochloric-Acid">Hydrochloric Acid 1M</h2> | <h2 id="Hydrochloric-Acid">Hydrochloric Acid 1M</h2> | ||
<p><u>Use:</u> Simulation of stomach acid conditions in bead experiments</p> | <p><u>Use:</u> Simulation of stomach acid conditions in bead experiments</p> | ||
| − | <p>u>Signal word:</u> Warning</p> | + | <p><u>Signal word:</u> Warning</p> |
<img src="https://static.igem.org/mediawiki/2016/f/f5/T--Oxford--HCL.png" style="float: right" width="40%"/> | <img src="https://static.igem.org/mediawiki/2016/f/f5/T--Oxford--HCL.png" style="float: right" width="40%"/> | ||
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<h2 id="Sodium-Hydroxide">Sodium Hydroxide</h2> | <h2 id="Sodium-Hydroxide">Sodium Hydroxide</h2> | ||
<p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p> | <p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p> | ||
| − | <p>u>Signal word:</u> Danger</p> | + | <p><u>Signal word:</u> Danger</p> |
<img src="https://static.igem.org/mediawiki/2016/d/d7/T--Oxford--SODIUM_HYDROXIDE.png" style="float: right" width="40%"/> | <img src="https://static.igem.org/mediawiki/2016/d/d7/T--Oxford--SODIUM_HYDROXIDE.png" style="float: right" width="40%"/> | ||
| Line 504: | Line 504: | ||
<h2 id="Glacial-Acetic-Acid">Glacial Acetic Acid</h2> | <h2 id="Glacial-Acetic-Acid">Glacial Acetic Acid</h2> | ||
<p><u>Use:</u> Bead experiments</p> | <p><u>Use:</u> Bead experiments</p> | ||
| − | <p>u>Signal word:</u> Danger</p> | + | <p><u>Signal word:</u> Danger</p> |
<img src="https://static.igem.org/mediawiki/2016/1/16/T--Oxford--Acetic_Acid.png" style="float: right" width="40%"/> | <img src="https://static.igem.org/mediawiki/2016/1/16/T--Oxford--Acetic_Acid.png" style="float: right" width="40%"/> | ||
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<h2 id="Calcium-Chloride">Calcium Chloride</h2> | <h2 id="Calcium-Chloride">Calcium Chloride</h2> | ||
<p><u>Use:</u> Forming beads from alginate. Ca2+ causes alginate phase change</p> | <p><u>Use:</u> Forming beads from alginate. Ca2+ causes alginate phase change</p> | ||
| − | <p>u>Signal word:</u> Warning</p> | + | <p><u>Signal word:</u> Warning</p> |
<img src="https://static.igem.org/mediawiki/2016/8/85/T--Oxford--CACL2.png" style="float: right"/> | <img src="https://static.igem.org/mediawiki/2016/8/85/T--Oxford--CACL2.png" style="float: right"/> | ||
Revision as of 00:15, 17 October 2016
Chemicals
Ethidium Bromide
Use: Staining DNA electrophoresis gels.
Signal word: Danger
Hazard statement(s)
- H302 Harmful if swallowed.
- H330 Fatal if inhaled.
- H341 Suspected of causing genetic defects.
Precautionary statement(s)
- P260 Do not breathe dust/fume/gas/mist/vapours/spray.
- P281 Use personal protective equipment as required.
- P284 Wear respiratory protection.
- P310 Immediately call a POISON CENTER or doctor/ physician.
TBE Buffer pH 8.3
Use: General buffer for DNA experiments.
Made of three chemicals: Tris,borate and EDTA.
Non-Hazardous
Copper Sulphate Pentahydrate:
Use: Induction of copper promoters and Cu2+ chelation assays.
Signal word: Warning
Hazard statement(s)
- H302 Harmful if swallowed.
- H315 Causes skin irritation.
- H319 Causes serious eye irritation.
- H410 Very toxic to aquatic life with long lasting effects.
Precautionary statement(s)
- P273 Avoid release to the environment.
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.Remove contact lenses, if present and easy to do. Continue rinsing.
- P501 Dispose of contents/container to an approved waste disposal plant.
Agarose
Use: DNA electrophoresis gel matrix
Non-Hazardous
L(+)-Arabinose
Use: Induction of pBAD expression
Non-Hazardous
Bathocuproinedisulfonic Acid Disodium Salt
Use: Chromophore in Cu+/Cu2+ detection assay
Non-Hazardous
L(+)-Sodium Ascorbate
Use: Reducing agent in Cu+/Cu2+ detection assay
Non-Hazardous
L-Glutathione
Use: Reducing agent in Cu+/Cu2+ detection assay.
Non-Hazardous
Chloramphenicol
Use: Antibiotic and positive selection marker for all plasmids except pBAD
Signal word: Danger
Hazard statement(s)
- H350 May cause cancer
Precautionary statement(s)
- P201 Obtain special instructions before use.
- P308 + P313 If exposed or concerned: Get medical advice/attention.
Ampicillin
Use: Antibiotic and positive selection marker for pBAD.
Signal word: Danger
Hazard statement(s)
- H315 Causes skin irritation
- H317 May cause an allergic skin reaction
- H319 Causes serious eye irritation
- H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled
- H335 May cause respiratory irritation
Precautionary statement(s)
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician
Tris pH 7.0 and 8.0
Use: General buffer for protein and copper assays
Non-Hazardous
Glycerol
Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer
Non-Hazardous
DL-Dithiothreitol
Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification
Signal word: Warning
Hazard statement(s)
- H302 Harmful if swallowed
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statement(s)
- P261 Avoid breathing dust
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Imidazole
Use: Elution of His-tagged target proteins from nickel bead column during protein purification
Signal word: Danger
Hazard statement(s)
- H302 Harmful if swallowed
- H314 Causes severe skin burns and eye damage
- H360D May damage the unborn child
Precautionary statement(s)
- P201 Obtain special instructions before use
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P310 Immediately call a POISON CENTER or doctor/physician
Nickel Affinity Beads
Use: Selection of His-tagged target proteins during protein purification
Non-Hazardous
Sodium Alginate
Use: Major component of bacterial delivery beads
Non-Hazardous
Crystal Violet
Use: Marker for bead quality assay
Signal word: Danger
Hazard statement(s)
- H302 Harmful if swallowed
- H318 Causes serious eye damage
- H350 May cause cancer
- H410 Very toxic to aquatic life with long lasting effects
Precautionary statement(s)
- P201 Obtain special instructions before use
- P273 Avoid release to the environment
- P280 Wear protective gloves/ eye protection/ face protection
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P308 + P313 If exposed or concerned: Get medical advice/attention
- P501 Dispose of contents/container to an approved waste disposal plant
Lysogeny Broth
Use: Media for general bacterial growth
Non-Hazardous
Phosphate Buffered Saline pH 7.4
Use: Dilution buffer for flow cytometry experiments
Non-Hazardous
Sodium Dodecyl Sulphate
Use: Detergent in protein identification gels
Signal word: Danger
Hazard statement(s)
- H228 Flammable solid
- H302 + H332 Harmful if swallowed or if inhaled
- H315 Causes skin irritation
- H318 Causes serious eye damage
- H335 May cause respiratory irritation
- H412 Harmful to aquatic life with long lasting effects
Precautionary statement(s)
- P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P280 Wear eye protection/face protection
- P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth
- P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor
- P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
Ethanol 100%
Use: solvent for chloramphenicol
Signal word: Danger
Hazard statement(s)
- H225 Highly flammable liquid and vapour
- H319 Causes serious eye irritation
Precautionary statement(s)
- P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
- P403 + P235 Store in a well-ventilated place. Keep cool
Transfection Buffer I (Manganese[II] Chloride)
Use: First soaking buffer in creating competent E. coli cells
Signal word: Danger
Hazard statement(s)
- H302 Harmful if swallowed
- H318 Causes serious eye damage
- H373 May cause damage to organs (Brain) through prolonged or repeated exposure if inhaled
- H411 Toxic to aquatic life with long lasting effects
Precautionary statement(s)
- P273 Avoid release to the environment
- P280 Wear eye protection/ face protection
- P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Rinse mouth
- P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician
- P391 Collect spillage
- P501 Dispose of contents/container to an approved waste disposal plant
Transfection Buffer II
Use: Second soaking buffer in creating competent E. coli cells
Non-Hazardous
Chitosan
Use: Coating for bacterial delivery beads
Non-Hazardous
InstantBlue
Use: Protein stain for protein identification gels
Signal word: Warning
Hazard statement(s)
- H290 May be corrosive to metals
- H315 Causes skin irritation
- H319 Causes serious eye irritation
Precautionary statement(s)
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
NEB Purple Loading Dye
Use: Stabilizer and marker for DNA gel electrophoresis
Non-Hazardous
Roche cOmplete EDTA-free Protease Inhibitor Cocktail
Use: Prevention of protein cleavage during protein purification
Signal word: Danger
Hazard statement(s)
- H314 Causes severe skin burns and eye damage Skin corrosion/irritation
Precautionary statement(s)
- P260 Do not breathe dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
- P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower
- P304 + P340 + P310 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/physician
- P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician
Hydrochloric Acid 1M
Use: Simulation of stomach acid conditions in bead experiments
Signal word: Warning
Hazard statement(s)
- H290 May be corrosive to metals
Sodium Hydroxide
Use: Simulation of duodenum conditions in bead experiments
Signal word: Danger
Hazard statement(s)
- H290 May be corrosive to metals
- H314 Causes severe skin burns and eye damage
Precautionary statement(s)
- P260 Do not breathe dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
- P304 + P340 + P310 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Monobasic Sodium Phosphate
Use: Simulation of duodenum conditions in bead experiments
Non-Hazardous
Glacial Acetic Acid
Use: Bead experiments
Signal word: Danger
Hazard statement(s)
- H226 Flammable liquid and vapour
- H314 Causes severe skin burns and eye damage
Precautionary statement(s)
- P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
- P260 Do not breathe dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
Calcium Chloride
Use: Forming beads from alginate. Ca2+ causes alginate phase change
Signal word: Warning
Hazard statement(s)
- H319 Causes serious eye irritation
Precautionary statement(s)
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
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