Difference between revisions of "Team:Nanjing-China/Demonstrate"
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<h6>The following list exhibits our key achievements in this project. Our design successfully works out under real conditions.</h6> | <h6>The following list exhibits our key achievements in this project. Our design successfully works out under real conditions.</h6> | ||
| − | <ul> | + | <ul style="list-style:square;"> |
<li>Achieved aerobic hydrogen production using our design.</li> | <li>Achieved aerobic hydrogen production using our design.</li> | ||
<li>Constructed and Tested recombinant with hydrogenase.</li> | <li>Constructed and Tested recombinant with hydrogenase.</li> | ||
<li>Constructed and Tested light-driven system based on OmpA-PbrR protein.</li> | <li>Constructed and Tested light-driven system based on OmpA-PbrR protein.</li> | ||
<li>Constructed desired structure of silicon encapsulation system.</li> | <li>Constructed desired structure of silicon encapsulation system.</li> | ||
| − | <li>Tested silicon encapsulation system with stability and viability.</li> | + | <li>Tested silicon encapsulation system with stability and viability.</li></ul> |
<p> </p> | <p> </p> | ||
<h6>Our design consists of three modules: hydrogenase, artificial PS system, silicon encapsulation. All three modules have gone through examination:</h6> | <h6>Our design consists of three modules: hydrogenase, artificial PS system, silicon encapsulation. All three modules have gone through examination:</h6> | ||
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<h6>Key achievements:</h6> | <h6>Key achievements:</h6> | ||
| − | <ul> | + | <ul style="list-style:square;"> |
<li>Constructed recombinant plasmid pET-28a with <em>hyaABCDEF</em> under T7 promoter</li> | <li>Constructed recombinant plasmid pET-28a with <em>hyaABCDEF</em> under T7 promoter</li> | ||
<li>Succeeded in induced overexpression of hydrogenase in recombinant <em>E.coli</em></li> | <li>Succeeded in induced overexpression of hydrogenase in recombinant <em>E.coli</em></li> | ||
<li>Conducted qualitative proof of the efficiency of hydrogenase using redox indicator WO<sub>3</sub></li> | <li>Conducted qualitative proof of the efficiency of hydrogenase using redox indicator WO<sub>3</sub></li> | ||
<li>Conducted quantitative proof of the efficiency of hydrogenase using gas chromatography | <li>Conducted quantitative proof of the efficiency of hydrogenase using gas chromatography | ||
| − | <ul> | + | <ul style="list-style:disc;"> |
<li>Completed standard curve of bulk H2 to measure the amount of H2 in samples</li> | <li>Completed standard curve of bulk H2 to measure the amount of H2 in samples</li> | ||
<li>Achieved double H2 production over native fermentation: in 600mL head space after 20h incubation: 14.71μmol in recombinant group against 6.88μmol in control group</li> | <li>Achieved double H2 production over native fermentation: in 600mL head space after 20h incubation: 14.71μmol in recombinant group against 6.88μmol in control group</li> | ||
Revision as of 01:58, 17 October 2016
Overview
The following list exhibits our key achievements in this project. Our design successfully works out under real conditions.
- Achieved aerobic hydrogen production using our design.
- Constructed and Tested recombinant with hydrogenase.
- Constructed and Tested light-driven system based on OmpA-PbrR protein.
- Constructed desired structure of silicon encapsulation system.
- Tested silicon encapsulation system with stability and viability.
Our design consists of three modules: hydrogenase, artificial PS system, silicon encapsulation. All three modules have gone through examination:
Hydrogenase
Overview
Hydrogenase is defined as the enzyme that catalyzes the reversible oxidization of hydrogen. Our target enzyme EcHyd-1 is encoded by a six-gene operon hyaABCDEF on the genome of E.coli. To achieve hydrogen production in E.coli, we induced overexpression of this native hydrogenase. All six genes will also be our Parts this year.
Key achievements:
- Constructed recombinant plasmid pET-28a with hyaABCDEF under T7 promoter
- Succeeded in induced overexpression of hydrogenase in recombinant E.coli
- Conducted qualitative proof of the efficiency of hydrogenase using redox indicator WO3
- Conducted quantitative proof of the efficiency of hydrogenase using gas chromatography
- Completed standard curve of bulk H2 to measure the amount of H2 in samples
- Achieved double H2 production over native fermentation: in 600mL head space after 20h incubation: 14.71μmol in recombinant group against 6.88μmol in control group
PbrR-based artificial PS system
Overview
The key element of light driven system in our design is induce-precipitated CdS semiconductor nanoparticles, constructed by OmpA-PbrR fused protein. Semiconductors, similar to natural PS systems, provide excited electrons under illumination. These electrons are then passed down to redox dye methyl viologen (MV) and eventually hydrogenases.
Key achievements:
