Difference between revisions of "Team:Missouri Rolla/Safety"

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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<a href="https://2016.igem.org/Team:Missouri_Rolla"><img id="logo2" src="https://static.igem.org/mediawiki/2016/d/d0/T--Missouri_Rolla--Logo_2016_red.png" /><img id="logo1" src="https://static.igem.org/mediawiki/2016/e/ea/T--Missouri_Rolla--Logo_2016.png" /></a>
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<div id="menu">
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  <a href="https://2016.igem.org/Team:Missouri_Rolla">Home</a>
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  <a href="https://2016.igem.org/Team:Missouri_Rolla">Project</a>
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  <a href="https://2016.igem.org/Team:Missouri_Rolla">Human Practices</a>
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  <a href="https://2016.igem.org/Team:Missouri_Rolla">Parts</a>
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  <a href="https://2016.igem.org/Team:Missouri_Rolla">Notebook</a>
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  <a href="https://2016.igem.org/Team:Missouri_Rolla/Team">Team</a>
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  <a href="https://2016.igem.org/Team:Missouri_Rolla/Safety">Safety</a>
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  <a href="https://2016.igem.org/Team:Missouri_Rolla">Attributions</a>
 
</div>
 
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  <p>All S&T iGEM members must be trained through our online and in-lab Lab Training Program before contributing to the project in lab. Our <a href="https://static.igem.org/mediawiki/2016/d/dc/T--Missouri_Rolla--LTM_2_5.pdf">Lab Training Manual</a>, which contains a variety of safety and procedure information, and our online lessons are currently being revised to better prepare future lab workers. The Lab Training Program culminates in the "mini-project," where trainees are given a small task and must produce a final product using parts from the iGEM DNA Distribution. Our goal is to train safe and productive lab workers by giving them a chance to try each procedure themselves under the supervision of an experienced member.</p>
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<p>We used <i>E. coli DH5α</i>, a non-pathogenic strain, for all assembly and as the chassis for testing. We attempted to express the mevalonate pathway to produce (E)-beta-ocimene, a safe terpene with pleasant scent. We also used <i>Streptomyces roseus</i>, another non-pathogenic strain. The genome of <i>S. roseus</i> contains two genes of interest, <i>leupA</i> and <i>leupB</i>, which are potentially involved in the production of leupeptin, a protease inhibitor. Our final safety form may be found <a href="https://2016.igem.org/Safety/Final_Safety_Form?team_id=2034
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<h5>Safe Project Design</h5>
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>here</a>.</p>
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<p>While assembling our parts, we used a lac promoter to control expression. A different approach would have to be taken for use outside of the lab, however. Ocimene indiscriminately inhibits microbial growth, so continuous production in a cave environment would have the potential to significantly impact the microbiome of bats' skin and the cave itself. We are investigating ways of sensing the causative agent of White-Nose Syndrome, <i>P. destructans</i>, as a potential future project. For real-world applications, sensing of the fungus would need to be coupled with either a kill switch or regulation of ocimene production. Before use for bat conservation, it should also be confirmed that ocimene has no adverse effects on bat species.</p>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Revision as of 04:24, 17 October 2016

All S&T iGEM members must be trained through our online and in-lab Lab Training Program before contributing to the project in lab. Our Lab Training Manual, which contains a variety of safety and procedure information, and our online lessons are currently being revised to better prepare future lab workers. The Lab Training Program culminates in the "mini-project," where trainees are given a small task and must produce a final product using parts from the iGEM DNA Distribution. Our goal is to train safe and productive lab workers by giving them a chance to try each procedure themselves under the supervision of an experienced member.

We used E. coli DH5α, a non-pathogenic strain, for all assembly and as the chassis for testing. We attempted to express the mevalonate pathway to produce (E)-beta-ocimene, a safe terpene with pleasant scent. We also used Streptomyces roseus, another non-pathogenic strain. The genome of S. roseus contains two genes of interest, leupA and leupB, which are potentially involved in the production of leupeptin, a protease inhibitor. Our final safety form may be found