Difference between revisions of "Team:Goettingen"

 
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<iframe frameborder="0" height="260" marginheight="0" marginwidth="0" src="https://www.betterplace.org/de/projects/21161-internationales-forscherteam-kampft-gegen-pilzinfektionen-igem-2014/widget" style="border: 0; padding:0; margin:0;" width="160">Informieren und spenden: <a href='https://www.betterplace.org/de/projects/21161-internationales-forscherteam-kampft-gegen-pilzinfektionen-igem-2014/widget' target='_blank'>„Internationales Forscherteam kämpft gegen Pilzinfektionen - iGEM 2014“</a> auf betterplace.org öffnen.</iframe>
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<h1> Welcome to iGEM 2016! </h1>
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<p>We intend to design, construct and introduce a synthetic Vitamin B<sub>12</sub> exporter (“Synporter”) into a production organism. Thereby, we aim for facilitated and higher yields in the industrial Vitamin B<sub>12</sub> production without requiring cell lysis.
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The B12 Synporter consists of a signal for a Twin Arginine Transporter (Tat) mediated export that is linked to a B<sub>12</sub>-binding domain. This construct will be expressed in <em>S. typhimurium</em> TA100, <em>R. planticola</em> and <em>S. blattae</em>. We will not only test these different production organisms but also different B<sub>12</sub>-binding domains.</p>
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<h3>Background</h3>
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<p>Vitamin B<sub>12</sub> is involved in metabolic functions in all organisms, and is therefore an essential nutrient. However, it can only be synthesized by some bacteria and archaea, thus, animals have to obtain it through their diet. Thereby, only animal products like meat and dairy contain B<sub>12</sub> in general. This B<sub>12</sub> was synthesized by microorganisms colonizing the gastrointestinal tract of those animals and accumulated in the animals’ tissues.
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Vitamin B<sub>12</sub> is one of the most essential biochemicals in the world, and its synthesis is extraordinarily complex. Since the chemical production of Vitamin B<sub>12</sub> requires 70 synthesis steps, it is far too technically challenging and expensive. Therefore, its production is facilitated by genetically engineered microorganisms. These are able to produce Vitamin B<sub>12</sub> in industrial amounts and achieve a high product quality. However, the produced Vitamin B<sub>12</sub> is harvested by cell lysis, which prevents a continuous production. The efficiency of production could be increased by exporting Vitamin B<sub>12</sub> outside the cells. To date, a natural cellular Vitamin B<sub>12</sub> exporter is unknown.</p>
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<h3>Relevance</h3>
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<p>An adult needs approximately 3.0 µg Vitamin B<sub>12</sub> per day which is essential for certain functions: it is involved in processes concerning synthesis of DNA, hormones and neurotransmitters and is involved in the formation of the nervous system and blood. Hence, a B<sub>12</sub> deficiency can cause diverse diseases like cancer, dementia, depression, pernicious anemia and polyneuropathy.</p>
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<p>Nowadays, many people do not eat meat or animals products at all. Therefore, these people have a high risk to suffer from B<sub>12</sub> deficiency if their diet does not provide enough B<sub>12</sub>.</p>
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<p>Vitamin B<sub>12</sub> is also used in the industry as it is needed in the biotechnical production of various organic substances. Furthermore, it is added to diverse daily products like toothpaste, fruit gum, non-diary milk or cleaning solution for contact lenses.</p>
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<h3>Further Aims</h3>
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<p>When we have successfully expressed our constructs in our productions organisms, we will try to maximize the Vitamin B<sub>12</sub> yield, and possibly find ways to utilize the periplasmic space to facilitate cytotoxic B<sub>12</sub> dependent reactions.</p>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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  <a href="https://2016.igem.org/Team:Goettingen/Team"><div class="pictures col-md-4"><img src="https://static.igem.org/mediawiki/2016/5/59/T--Goettingen--Team-icon.png" alt=""/></a></div>
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  <div class="paragraph col-md-8"><p>We are a group of students from the University of Göttingen studying in the international Master Program "Microbiology and Biochemistry", and one student from the Bachelor Program "Biology" as creative assistant.</p></div>
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<h5> Wiki template information </h5>
 
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
  
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  <a href="https://2016.igem.org/Team:Goettingen/Description"><div class="pictures col-md-4"><img src="https://static.igem.org/mediawiki/2016/7/76/T--Goettingen--Project_icon.png" alt=""/></a></div>
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  <div class="paragraph col-md-8"><p>We intend to design, construct and introduce a synthetic Vitamin B<sub>12</sub> exporter (= B<sub>12</sub> Synporter) in a B<sub>12</sub> production organism. Thereby, we aim for facilitated and higher yields in the industrial Vitamin B<sub>12</sub> production without requiring cell lysis.</p></div>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
 
  
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  <a href="https://2016.igem.org/Team:Goettingen/Experiments"><div class="pictures col-md-4"><img src="https://static.igem.org/mediawiki/2016/b/b9/T--Goettingen--Experiments.png" alt=""/></a></div>
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  <div class="paragraph col-md-8"><p>Take a look on our project roadmap to build and test our B<sub>12</sub> Synporter, and how we designed our experiments.</p></div>
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  <div class="paragraph col-md-8"><p>In the meaning of open science, we want you to be able to follow any single step we did in the lab. Come and follow our work.</p></div>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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  <a href="https://2016.igem.org/Team:Goettingen/Results"><div class="pictures col-md-4"><img src="https://static.igem.org/mediawiki/2016/c/c0/T--Goettingen--Results.png" alt=""/></a></div>
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  <div class="paragraph col-md-8"><p>See which delicious fruits the tree of our hard work carries in the end.</p></div>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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  <a href="https://2016.igem.org/Team:Goettingen/Human_Practices"><div class="pictures col-md-4"><img src="https://static.igem.org/mediawiki/2016/d/d9/T--Goettingen--HP_icon.png" alt=""/></a></div>
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  <div class="paragraph col-md-8"><p>Besides the lab work, iGEM also reaches out of the lab. Follow our human practice actions, scientific representation.</p></div>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
  
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<li><a href="https://twitter.com/iGEM_Goettingen"><img src="https://static.igem.org/mediawiki/2016/c/c8/T--Goettingen--Twitter_icon.png" width="32px"/>&nbsp; Twitter</a></li>
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  <li><a href="mailto:igem2016@gwdg.de"><img src="https://static.igem.org/mediawiki/2016/4/42/T--Goettingen--Mail_icon.png" width="32px"/>&nbsp; E-Mail</a></li>
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  <li><a href="http://igem2016.uni-goettingen.de/"><img src="https://static.igem.org/mediawiki/2016/f/fb/T--Goettingen--Web_icon.png" width="32px"/>&nbsp; Homepage</a></li>
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  <li><a href="http://appmibio.uni-goettingen.de/"><img src="https://static.igem.org/mediawiki/2016/d/d0/T--Goettingen--Post.png" width="32px"/>&nbsp; Address</a></li>
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  iGEM Team Göttingen 2016<br>
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  Institut für Mikrobiologie <br>
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  und Genetik<br>
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  AG Prof. Dr. Rolf Daniel<br>
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  Grisebachstr. 8<br>
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  37077 Göttingen<br>
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  <li> <a href="https://2016.igem.org/Team:Goettingen/Description">Description</a></li>
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  <li> <a href="https://2016.igem.org/Team:Goettingen/Experiments"> Experiments </a></li>
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  <li> <a href="https://2016.igem.org/Team:Goettingen/Proof">Proof of Concept</a></li>
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  <li> <a href="https://2016.igem.org/Team:Goettingen/Results"> Results </a></li>
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  <li> <a href="https://2016.igem.org/Team:Goettingen/Notebook"> Notebook </a></li>
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  <li class="menu_item"> <div class="icon plus"></div> Parts
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  <li> <a href="https://2016.igem.org/Team:Goettingen/Parts">Parts</a></li>
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  <li> <a href="https://2016.igem.org/Team:Goettingen/Basic_Part">Basic Parts </a></li>
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  <li class="menu_item"> <a href="https://2016.igem.org/Team:Goettingen/Safety">Safety</a> </li>
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  <li class="menu_item"> <a href="https://2016.igem.org/Team:Goettingen/Attributions">Attributions</a></li>
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  <li> <a href="https://2016.igem.org/Team:Goettingen/Human_Practices">Human Practices</a></li>
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  <li> <a href="https://2016.igem.org/Team:Goettingen/HP/Silver">HP on the Road</a></li>
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Latest revision as of 17:38, 17 October 2016


Welcome to Our Wiki Page

Our Team

We are a group of students from the University of Göttingen studying in the international Master Program "Microbiology and Biochemistry", and one student from the Bachelor Program "Biology" as creative assistant.

Our Project

We intend to design, construct and introduce a synthetic Vitamin B12 exporter (= B12 Synporter) in a B12 production organism. Thereby, we aim for facilitated and higher yields in the industrial Vitamin B12 production without requiring cell lysis.

Experiments

Take a look on our project roadmap to build and test our B12 Synporter, and how we designed our experiments.

Notebook

In the meaning of open science, we want you to be able to follow any single step we did in the lab. Come and follow our work.

Results

See which delicious fruits the tree of our hard work carries in the end.

Outside the Lab

Besides the lab work, iGEM also reaches out of the lab. Follow our human practice actions, scientific representation.


Contact


iGEM Team Göttingen 2016
Institut für Mikrobiologie
und Genetik
AG Prof. Dr. Rolf Daniel
Grisebachstr. 8
37077 Göttingen

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