Difference between revisions of "Team:OUC-China/Protocol"
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<div class="oucBanner"> | <div class="oucBanner"> | ||
<h3 class="text-center">Cistrons Concerto</h3> | <h3 class="text-center">Cistrons Concerto</h3> | ||
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| − | <h3>Thanks</h3 | + | <h3>Thanks:</h3> |
| − | + | <p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p> | |
| − | <p | + | <p>2.NEW ENGLAND Biolabs</p> |
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</div> | </div> | ||
<div class="col-md-5 Contact"> | <div class="col-md-5 Contact"> | ||
<h3>Contact us:</h3> | <h3>Contact us:</h3> | ||
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<p><b>E-mail</b>: oucigem@163.com</p> | <p><b>E-mail</b>: oucigem@163.com</p> | ||
<p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p> | <p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p> | ||
Revision as of 10:19, 18 October 2016
1. Elementary Operation
1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction
2. Plasmid Construction
1) Gibson
2) DNA cassettes
3. Systems detection
1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol
Cistrons Concerto
Thanks:
1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
2.NEW ENGLAND Biolabs
Contact us:
E-mail: oucigem@163.com
Designed and built by @ Jasmine Chen and @ Zexin Jiao
We are OUC-iGEM
