Difference between revisions of "Team:KAIT Japan/Safety"
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<h1>Safety</h1> | <h1>Safety</h1> | ||
| − | <h4>Q1. What is our chassis organism?</h4> | + | <h4>Q1. What is our chassis organism?</h4> |
</div> | </div> | ||
| + | <h4>A1．E.coli （DH-5α） and Acetobacter xylinum. | ||
| + | <br> | ||
| + | <p style="padding-left:3em"> | ||
| + | We use DH-5α for DNA cloning and we also use the E.coli as a model microorganisms to check the function of cloned DNA. We were going to transform A. xylinum. A. xylinum was used in an industry field and is normally present in the environment. | ||
| + | </p></h4> | ||
| + | <br> | ||
| + | <br> | ||
<div class="column full_size"> | <div class="column full_size"> | ||
| − | <h4></h4> | + | <h4> Q2. What risks does our project pose at the laboratory stage? What actions are we takinｇ to reduce those risks? </h4> |
| + | </div> | ||
| + | <h4>A2.・We wear a lab coat and gloves when we use microorganisms. | ||
| + | <br> | ||
| + | ・We have sterilized our used materials by autoclave, ethanol. | ||
| + | <br> | ||
| + | ・We have experimented in clean bench to prevent our organism from escaping from lab. | ||
| + | <br> | ||
| + | ・We sterilized all waste before we throw away. | ||
| + | <br> | ||
| + | ・We have a P2 level laboratory and we use the room. | ||
| + | </h4> | ||
</div> | </div> | ||
| + | <h4></h4> | ||
</html> | </html> | ||
Revision as of 13:11, 18 October 2016
Safety
Q1. What is our chassis organism?
A1．E.coli （DH-5α） and Acetobacter xylinum.
We use DH-5α for DNA cloning and we also use the E.coli as a model microorganisms to check the function of cloned DNA. We were going to transform A. xylinum. A. xylinum was used in an industry field and is normally present in the environment.
