Difference between revisions of "Team:Manchester/Model/MechanismUncertainty"

m
m
Line 60: Line 60:
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
     <td>Irreversible Michaelis-Menten Kinetics</td>
+
     <td>Irreversible Michaelis-Menten</td>
     <td>Irreversible Michaelis-Menten Kinetics</td>
+
     <td>Irreversible Michaelis-Menten</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
     <td>Reversible Michaelis-Menten Kinetics</td>
+
     <td>Reversible Michaelis-Menten</td>
     <td>Reversible Michaelis-Menten Kinetics</td>
+
     <td>Reversible Michaelis-Menten</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
     <td>Uni-Bi Kinetics</td>
+
     <td>UNI-BI Reversible Michaelis-Menten</td>
     <td>Bi-Uni Kinetics</td>
+
     <td>BI-UNI Reversible Michaelis-Menten</td>
 
   </tr>
 
   </tr>
  

Revision as of 13:12, 18 October 2016

Manchester iGEM 2016

Network Mechanism Analysis


Contents

Overview and Motivation
Methodology
Results
Conclusions

Overview and Motivation

During discussions with the experimental team it became clear to us that the exact reaction mechanism in place was not clearly understood. By modelling a range of different potential mechanisms and comparing the outputs to experimental data we could draw conclusions about the accuracy of the mechanisms and hence refine the model in an effort to produce more accurate predictions and improve our understanding of the system.

There were a few different mechanisms tested in this analysis

Reaction One (GOx) Reaction Two (HRP)
Irreversible Michaelis-Menten Irreversible Michaelis-Menten
Reversible Michaelis-Menten Reversible Michaelis-Menten
UNI-BI Reversible Michaelis-Menten BI-UNI Reversible Michaelis-Menten

Return to top of page

Methodology

For each of the combinations of potential rate equations for each step the following was undertaken:

The model was run with many iterations.
The concentration at steady state for each iteration was recorded and plotted in a histogram.
Experimental steady state data was then compared to these histograms.


Return to top of page

Results

PLACEHOLDER FOR GRAPHS


Return to top of page

Conclusions

From the graphs you can see that...



Return to top of page
Return to overview