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| <h2>Agenda:</h2> | | <h2>Agenda:</h2> |
| | | |
| + | <ul> |
| + | <li>Constructs</li> |
| + | <li>Practice presentations</li> |
| + | <li>Ligation</li> |
| + | <li>Meeting with grad students</li> |
| + | </ul> |
| + | <h2>Results:</h2> |
| + | <p>Cloning practice: Redid the gel from yesterday. This time, we didn't run the gel quite long enough and the bands didn't separate that much.</p> |
| + | <h2>Tasks:</h2> |
| + | <div class="row"> |
| + | <div class="col-sm-2"> |
| + | <p>Michelle</p> |
| + | </div> |
| + | <div class="col-sm-10"> |
| + | <ul> |
| + | <li>Website research</li> |
| + | <li>Golden Gate preparation</li> |
| + | </ul> |
| + | </div> |
| + | </div> |
| + | <div class="row"> |
| + | <div class="col-sm-2"> |
| + | <p>Sara</p> |
| + | </div> |
| + | <div class="col-sm-10"> |
| + | <ul> |
| + | <li>Ran the gel again</li> |
| + | <li>Emailed Bernd</li> |
| + | <li>Looked into choosing homologous regions to add onto the signaling sequences, ran into problems with annealing temp </li> |
| + | </ul> |
| + | </div> |
| + | </div> |
| + | <div class="row"> |
| + | <div class="col-sm-2"> |
| + | <p>Tasfia</p> |
| + | </div> |
| + | <div class="col-sm-10"> |
| + | <ul> |
| + | <li>Attempted to make overhangs on SS parts, but the Tm was too high for optimal Gibson assembly with really long overhangs</li> |
| + | <li>Cloning practice</li> |
| + | </ul> |
| + | </div> |
| + | </div> |
| + | <div class="row"> |
| + | <div class="col-sm-2"> |
| + | <p>Tyler</p> |
| + | </div> |
| + | <div class="col-sm-10"> |
| + | <ul> |
| + | <li>Finalized homology arms for split Cas9</li> |
| + | <li>Designed RTW PCR primers of tet backbone</li> |
| + | <li>Received Promega’s order of restriction</li> |
| + | </ul> |
| + | </div> |
| + | </div> |
| + | <div class="row"> |
| + | <div class="col-sm-2"> |
| + | <p>Jordan</p> |
| + | </div> |
| + | <div class="col-sm-10"> |
| + | <ul> |
| + | <li>Helped with gels</li> |
| + | <li>Crowdfunding got approved</li> |
| + | <li>Edited crowdfunding page</li> |
| + | <li>Presented to grad students </li> |
| + | </ul> |
| + | </div> |
| + | </div> |
| + | <div class="row"> |
| + | <div class="col-sm-2"> |
| + | <p>Paul</p> |
| + | </div> |
| + | <div class="col-sm-10"> |
| + | <ul> |
| + | <li>Update presentation with GFP fusions</li> |
| + | <li>Researched GFP fusions</li> |
| + | <li>Upload sfGFP to Genome Compiler </li> |
| + | </ul> |
| + | </div> |
| + | </div> |
| + | |
| + | </article> |
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