Difference between revisions of "Team:UPO-Sevilla"

 
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              <div class="span3"><a class="brand" href="#"><img src="https://static.igem.org/mediawiki/2016/9/94/T--UPO-Sevilla--upoescudo.jpg" width="200" height="400"></a></div>
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<h2> Welcome to iGEM 2016! </h2>
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              <div class="span9">
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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                          <a class="dropdown-toggle" data-toggle="dropdown">Team<b class="caret"></b></a>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Sevilla">Our city</a></li>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Team">Members</a></li>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Attributions">Attributions</a></li>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Description">Description</a></li>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Design">Design</a></li>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Proof">Proof of Concept</a></li>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Demonstrate">Demonstrate</a></li>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Notebook">Notebook</a></li>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Basic_Part">Basic Parts</a></li>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Composite_Part">Composite Parts</a></li>
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  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Part_Collection">Part Collection</a></li>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Protocols">Protocols</a></li>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Experiments">Experiments</a></li>
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  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Safety">Safety</a></li>
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<h5>Before you start: </h5>
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                          <a class="dropdown-toggle" data-toggle="dropdown">Dry Lab<b class="caret"></b></a>
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<p> Please read the following pages:</p>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Software">Software</a></li>
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<li> <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Model">Modeling</a></li>
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
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                        <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Collaborations">Collaborations</a></li>
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                          <a class="dropdown-toggle" data-toggle="dropdown">Human Practices<b class="caret"></b></a>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/HP/Silver">HP Silver</a></li>
  
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Integrated_Practices">Integrated Practices</a></li>
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<div class="highlight">
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/HP/Gold">HP Gold</a></li>
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<h5> Styling your wiki </h5>
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                                  <li><a href="https://2016.igem.org/Team:UPO-Sevilla/Engagement">Engagement</a></li>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<h5> Description of our project </h5>
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<p>Bacteria are an amazing field to work on. They can develop a wide variety of functionalities and properties in a very short time, being easy to work with and extremely versatile. On this basis, we wanted to take advantage of one of the most remarkable characteristics of the bacteria we work with, Pseudomonas putida – the ability to form biofilms. These structures are composed of communities of bacteria which are integrated in an extracellular polysaccharide matrix that protects them from several kinds of stress. In addition, they have an increased metabolism, a feature that is often required in industrial production. </p>
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<p>Therefore, we thought that it would be a good application to try and do some bioremediation with these strong and powerful bacteria. First, we thought about glyphosate, an herbicide that has been dproved to be toxic to human. But due to technical difficulties, we could not continue with that idea. Instead, we found out about glycerol. It is being overproduced in the biofuel industry, and it is starting to become an environmental problem. So we started to model how our bacteria would eat that glycerol, using biofilms for that. But, is there anything else we can do once the bacteria are eating glycerol? We decided that there could be a compound we could produce with these biological reactors, and our choice was propionate. It is widely used in a large variety of fields, easy to excrete from our bacteria and, according to the model, easy to produce. Let’s get started!</p>  
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<a href="https://www.facebook.com/profile.php?id=100006118518232&fref=ts" target="_blank" title="Facebook official page"><img src="https://static.igem.org/mediawiki/igem.org/thumb/8/8b/FaceBook-icon.png/120px-FaceBook-icon.png" width="30" height="30"></a>
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<a href=https://twitter.com/iGEM_UPO target="_blank" title="Twitter official page"><img src="https://static.igem.org/mediawiki/igem.org/thumb/1/1a/Twitter_logo.png/120px-Twitter_logo.png" width="30" height="30"></a>
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<a href="https://www.instagram.com/igemupo/" target="_blank" title="Instagram official page"><img src="https://static.igem.org/mediawiki/2016/thumb/f/f7/Instagram.jpeg/120px-Instagram.jpeg.png" width="30" height="30"></a>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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                      <h1 style="text-align:center"><b>BIOFILM TRANSFORMERS</b></h1>
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                      <h3 class="lead" style="text-align:center">Give us a biofilm and we will change the world.</h3>
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                <div class="span6"> <img src="https://static.igem.org/mediawiki/2016/f/fd/T--UPO-Sevilla--logo_uposevilla.jpg" style="width:300px;height:300px;padding-top:50px;padding-bottom:100px;padding-left:100px"></div>
  
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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                      <img src="img/Giralda.png" style="width:550px;height:330px">
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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        <h1 style="text-align:center">Abstract of our Project</h1>
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            <p style="text-align:justify;font-size:15px;padding-bottom:10px"> Glycerol is a byproduct from the biodiesel industry that contaminates the environment and constitutes a real problem worldwide. To help eliminating this compound, we use it as a carbon source for bacteria. We take advantage of <i>Pseudomonas putida</i>, a bacterium capable of adhering to substrate and aggregate to form communities or biofilms. In this state, bacteria are more efficient, robust and resistant than those in suspension and are excellent catalysts of biotechnological use. By using glycerol as the carbon source, bacteria forming biofilms can produce compounds that are beneficial for human beings.</p>
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      <p style="text-align:justify;font-size:15px;padding-bottom:10px">We genetically manipulate <i>P. putida</i> based on in silico predictions to increase glycerol assimilation and to control formation and dispersal of biofilms. Having that, there are a lot of molecules that can be produced by introducing the genes needed for it. We propose producing propionate, as it is a small and simple molecule easy to secrete and with a lot of uses in different industries.</p>
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        <p style="text-align:justify;font-size:15px">This way, we develop a production platform, eliminating a contaminating compound and producing a useful one in an efficient manner.</p>
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<a href="https://www.youtube.com/channel/UCXUAle_ywH8F127dWBYrhgw" target="_blank" title="Youtube official page"><img src="https://static.igem.org/mediawiki/2016/a/ac/T--UPO-Sevilla--youtube.jpg" width="60" style="height:50px"</a>
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Latest revision as of 02:02, 19 October 2016

Abstract of our Project

Glycerol is a byproduct from the biodiesel industry that contaminates the environment and constitutes a real problem worldwide. To help eliminating this compound, we use it as a carbon source for bacteria. We take advantage of Pseudomonas putida, a bacterium capable of adhering to substrate and aggregate to form communities or biofilms. In this state, bacteria are more efficient, robust and resistant than those in suspension and are excellent catalysts of biotechnological use. By using glycerol as the carbon source, bacteria forming biofilms can produce compounds that are beneficial for human beings.

We genetically manipulate P. putida based on in silico predictions to increase glycerol assimilation and to control formation and dispersal of biofilms. Having that, there are a lot of molecules that can be produced by introducing the genes needed for it. We propose producing propionate, as it is a small and simple molecule easy to secrete and with a lot of uses in different industries.

This way, we develop a production platform, eliminating a contaminating compound and producing a useful one in an efficient manner.