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Composite Parts

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/6/63/T--UPO-Sevilla--nahRPsalLapG.png" class="media-object" alt='' style="width:200px;height:194px" /></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. lapG (see BBa_K1973000) is expressed from the Psal promoter in the presence of this molecule.</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/7/7b/T--UPO-Sevilla--nahRPsalYhjH.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. yhjH (see BBa_K1973034) is expressed from the Psal promoter in the presence of this molecule.</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/7/74/T--UPO-Sevilla--nahRPsalNasF.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter.</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/b/b7/T--UPO-Sevilla--nahRPsalNasFLapG.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. lapG (see BBa_K1973000) is expressed from the Psal promoter in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter.</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/1/12/T--UPO-Sevilla--nahRPsalNasFYhjH.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. yhjH (see BBa_K1973034) is expressed from the Psal promoter in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter.</p>

<div class="media" style="margin-bottom:70px">

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/e/e7/T--UPO-Sevilla--nahRPsalPleD.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. pleD* (see BBa_K1973016) is expressed from the Psal promoter in the presence of this molecule. </p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/2/24/T--UPO-Sevilla--nahRPsalNasFPleD.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter in the presence of salicylate. pleD* (see BBa_K1973016) is expressed from the Psal promoter (see BBa_J61051) in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter.</p>

<div class="media">

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/1/17/T--UPO-Sevilla--nahRPsalXylS2.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. xylS2 (BBa_K1973002) is expressed from the Psal promoter in the presence of this molecule. This module provides a tight expression regulation by salicylate thanks to a two-step (NahR and XylS2) system.</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/0/00/T--UPO-Sevilla--nahRPsalNasFXylS2.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. xylS2 (BBa_K1973002) codifies the modified XylS2 protein from Pseudomonas putida MT1, which is a transcriptional activator of the Pm promoter in response to salicylate. This module provides a tight expression regulation by salicylate thanks to a two-step (NahR and XylS2) system.</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/f/fc/T--UPO-Sevilla--PmGlpF.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">Pm1 (BBa_K1973013) is one variant of the promoter for the xylS/Pm expression system. It was obtained by site-directed mutagenesis PCR from the Pseudomonas putida plasmid pMPO52 in order to remove illegal restriction sites. The expression of this promoter is activated by XylS or XylS2 in the presence of benzoate and salicylate, respectively. In this construct, Pm1 controls the expression of glpF (BBa_K1973026), a glycerol transporter.</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/1/1c/T--UPO-Sevilla--Tn7nplapg.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. lapG (see BBa_K1973000) is expressed from the Psal promoter in the presence of this molecule. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/9/98/T--UPO-Sevilla--Tn7npnlapg.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter in the presence of salicylate. lapG (see BBa_K1973000) is expressed from the Psal promoter (see BBa_J61051) in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/3/35/T--UPO-Sevilla--Tn7npnyhjh.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter in the presence of salicylate. yhjH (see BBa_K1973034) is expressed from the Psal promoter (see BBa_J61051) in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/c/c3/T--UPO-Sevilla--Tn7npnpled.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter in the presence of salicylate. pleD* (see BBa_K1973016) is expressed from the Psal promoter (see BBa_J61051) in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/c/c3/T--UPO-Sevilla--Tn7npn.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. nasF (see Bba_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/0/05/T--UPO-Sevilla--Tn7npnxyls2.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. xylS2 (BBa_K1973002) codifies the modified XylS2 protein from Pseudomonas putida MT1, which is a transcriptional activator of the Pm promoter in response to salicylate. This module provides a tight expression regulation by salicylate thanks to a two-step (NahR and XylS2) system. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/6/69/T--UPO-Sevilla--Tn7npyhjh.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. yhjH (see BBa_K1973034) is expressed from the Psal promoter in the presence of this molecule. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/8/8c/T--UPO-Sevilla--Tn7nppled.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. pleD* (BBa_K1973016) is expressed from the Psal promoter in the presence of this molecule. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/2/2a/T--UPO-Sevilla--Tn7npxyls2.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. xylS2 (Bba_K1973002) codifies the modified XylS2 protein from Pseudomonas putida MT1, which is a transcriptional activator of the Pm promoter in response to salicylate. This module provides a tight expression regulation by salicylate thanks to a two-step (NahR and XylS2) system. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<div class="media" style="margin-bottom:120px">

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/4/45/T--UPO-Sevilla--Tn7np.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/b/ba/T--UPO-Sevilla--Tn7npglpf.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. glpF (BBa_K1973026) is expressed from the Psal promoter in the presence of this molecule. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/d/db/T--UPO-Sevilla--Tn7npnglpf.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. glpF (BBa_K1973026) is expressed from the Psal promoter in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/5/59/T--UPO-Sevilla--Tn7npgfp.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. GFP (BBa_E0040) is expressed from the Psal promoter in the presence of this molecule, providing a useful report module to test the expression system. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/2/24/T--UPO-Sevilla--Tn7npepi.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. epi (BBa_K1973030) is expressed from the Psal promoter in the presence of this molecule. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/c/cb/T--UPO-Sevilla--Tn7npnepi.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. epi (BBa_K1973030) is expressed from the Psal promoter in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>

<a href="#" class="pull-left"><img src="https://static.igem.org/mediawiki/2016/0/0e/T--UPO-Sevilla--Tn7nppledyhjh.png" class="media-object" alt='' style="width:200px;height:194px"/></a>

<p style="font-size:17px">nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. pleD* (BBa_K1973016) and glpF (BBa_K1973026) are expressed from the Psal promoter in the presence of this molecule. Therefore, this construct encodes the genes that are necessary for biofilm induction and glycerol uptake, connecting both activities under the same expression and regulation system. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).</p>