Difference between revisions of "Team:Hong Kong HKU"

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{{Hong_Kong_HKU}}
 
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[[File:Logo_HKU_256x256.jpg|center]]
 
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{{HKU iGEM Team 2016}}
 
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<html>
 
<html>
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<div class="column full_size" >
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<meta http-equiv="X-UA-Compatible" content="IE=edge">
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<h2> HKU iGEM Team 2016 </h2>
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<meta name="viewport" content="width=device-width, initial-scale=1.0">
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<p> We are ready to start the iGEM season! </p>
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<style>
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#sideMenu
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{display:none; /* Disable the display of the annoying side main menu*/}
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#top_title
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{display:none; /* Disable the annoying title*/}
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#content
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{padding:0px; width:90%; margin-left:5%; margin-right:5%;background-color: rgba(255,255,255,0);}
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body
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{margin: 0;
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font-family: "Helvetica Neue", Helvetica, Arial, sans-serif;
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font-size: 24px;
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color: #333333;
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background-color: #fafafa;
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background-image:url(https://static.igem.org/mediawiki/2016/a/ae/T--Hong_Kong_HKU--Background_image.png);
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background-position:center center;
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background-repeat:no-repeat;
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-moz-background-size: cover;
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background-size: cover;
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background-attachment:fixed;}
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.container
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{background-color: rgba(255,255,255,0.6);
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padding-top:80px;}
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p {font-size: 16px;}
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h1,h2,h3,h4,h5,h6 {color: #282828;}
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h1 {font-size:72px;} h2 {font-size:48px;} h3 {font-size:36px;}
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h4 {font-size:32px;} h5 {font-size:28px;} h6 {font-size:24px;}
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.panel-body{background-color:rgba(255, 255, 255, 0) !important;}
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.panel-heading{background-color:rgba(255, 255, 255, 0.7)  !important;}
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.panel-group{background-color:rgba(255, 255, 255, 0)  !important;}
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.panel-transparent{background-color:rgba(255, 255, 255, 0) !important;}
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</style>
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<link href="css/bootstrap.css" rel="stylesheet" type="text/css">
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<head>
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<meta charset="utf-8">
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<meta http-equiv="X-UA-Compatible" content="IE=edge">
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<meta name="viewport" content="width=device-width, initial-scale=1">
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<link href="https://2016.igem.org/Team:Hong_Kong_HKU/css/default?action=raw&ctype=text/css" type="text/css" rel="stylesheet">
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<link href="https://2016.igem.org/Team:Hong_Kong_HKU/css/custom?action=raw&ctype=text/css" type="text/css" rel="stylesheet">
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</head>
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</html>
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{{Hong_Kong_HKU/Header}}
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<html>
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<body>
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<div class="container" align="center">
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    <h2>Welcome to HKU iGEM HomePage!</h2><br>
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    <div class="panel-group" id="BackgroundContent" role="tablist" aria-multiselectable="true">
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      <div class="panel panel-transparent">
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        <div class="panel-heading" role="tab">
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          <h4 class="panel-title"><h3>Background</h3></h4>
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        </div>
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        <div id="Background" class="panel-collapse collapse in">
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          <div class="panel-body">
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          <p class="text-justify" align="left"><font size="3">
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          Since last decade, microRNAs have been identified as promising biomarkers for specific diseases, one common type is cancer.
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          miRNA, usually of around 22 nucleotides long, are made inside our body via complex mechanisms.
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          They play important roles in gene regulation through several ways, such as binding with messenger-RNA (mRNA) to inhibit translation and speeding up mRNA degradation to cause gene silencing.
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          Dysregulation of miRNA expression may lead to under- or over-expression of genes and hence diseases.<br>
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          G-quadruplexes (Gq) are formed by 4 strands of DNA made up of Guanine bases.
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          When Gq forms a complex with Hemin, it exhibits peroxidase activity and functions as a DNAzyme.
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          Its catalytic activity is utilized in many DNA nanostructures where a colour change is produced by target-induced conformational change.<br>
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          During the strand displacement reactions, two strands with partly or fully complementary sequences hybridize to each other,
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          displacing one or more pre-hybridised strands. This process is initiated at a single-stranded site called a ‘toehold’.
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          Seeing this as a commonly-employed reaction in DNA nanostructure designs, we of course include this as one of our the main properties we have in our designs.
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      </font></p>
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          </div>
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        </div>
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      </div>
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      <div class="panel panel-transparent">
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        <div class="panel-heading">
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          <h4 class="panel-title"><h3>Abstract</h3></h4>
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        </div>
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        <div id="Abstract" class="panel-collapse collapse in">
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          <div class="panel-body">
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          <h4>In vivo synthesis of DNA nanostructures for disease diagnosis through miRNA-induced structural transformation</h4>
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            <p class="text-justify" align="left"><font size="3">
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            DNA has emerged as a promising material for the creation of novel functional nanostructures.
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            Here we present DNA nanostructures capable of simultaneous detection of multiple microRNA (miRNA) targets which are identified as promising disease biomarkers.
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            Logic gates can be easily incorporated into our designs to test various combinations of miRNA targets.
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            G-quadruplexes form when the specified target hybridizes with the probe, generating fluorescence in the presence of substrate.
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            We endeavor to demonstrate intracellular synthesis, self-assembly and functioning of our nanostructures inside E. coli.
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            Our constructs open up new possibilities in future research on DNA nanotechnologies as diagnostic tools, and promote the applications of miRNA testing in clinical conditions.
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          </font></p>
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          </div>
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        </div>
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      </div>
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  </div>
  
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<h5>Links: </h5>
 
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<p> Please read the following pages:</p>
 
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<ul>
 
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
 
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
 
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
 
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</ul>
 
 
</div>
 
</div>
  
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<div class="column half_size" >
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<!-- footer -->
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<div class="highlight">
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<footer class="text-center"></footer>
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<h5> Styling your wiki </h5>
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<script src="https://2016.igem.org/Team:Hong_Kong_HKU/JS/jQuery?action=raw&ctype=text/javascript" type="text/javascript"></script>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<script src="https://2016.igem.org/Template:Hong_Kong_HKU/js/script?action=raw&ctype=text/javascript" type="text/javascript"></script>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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</body>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to view the example! </a></p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<div class="button_click"  onClick=" parent.location= 'https://2016.igem.org/Special:Upload '"> 
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UPLOAD FILES
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{{Hong_Kong_HKU/Footer}}

Latest revision as of 05:47, 19 October 2016

Welcome to HKU iGEM HomePage!


Since last decade, microRNAs have been identified as promising biomarkers for specific diseases, one common type is cancer. miRNA, usually of around 22 nucleotides long, are made inside our body via complex mechanisms. They play important roles in gene regulation through several ways, such as binding with messenger-RNA (mRNA) to inhibit translation and speeding up mRNA degradation to cause gene silencing. Dysregulation of miRNA expression may lead to under- or over-expression of genes and hence diseases.
G-quadruplexes (Gq) are formed by 4 strands of DNA made up of Guanine bases. When Gq forms a complex with Hemin, it exhibits peroxidase activity and functions as a DNAzyme. Its catalytic activity is utilized in many DNA nanostructures where a colour change is produced by target-induced conformational change.
During the strand displacement reactions, two strands with partly or fully complementary sequences hybridize to each other, displacing one or more pre-hybridised strands. This process is initiated at a single-stranded site called a ‘toehold’. Seeing this as a commonly-employed reaction in DNA nanostructure designs, we of course include this as one of our the main properties we have in our designs.

Abstract

In vivo synthesis of DNA nanostructures for disease diagnosis through miRNA-induced structural transformation

DNA has emerged as a promising material for the creation of novel functional nanostructures. Here we present DNA nanostructures capable of simultaneous detection of multiple microRNA (miRNA) targets which are identified as promising disease biomarkers. Logic gates can be easily incorporated into our designs to test various combinations of miRNA targets. G-quadruplexes form when the specified target hybridizes with the probe, generating fluorescence in the presence of substrate. We endeavor to demonstrate intracellular synthesis, self-assembly and functioning of our nanostructures inside E. coli. Our constructs open up new possibilities in future research on DNA nanotechnologies as diagnostic tools, and promote the applications of miRNA testing in clinical conditions.


Copyright © 2016 HKU iGEM. All rights reserved.