Difference between revisions of "Team:KAIT Japan/Labnote"
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{{KAIT_Japan}} | {{KAIT_Japan}} | ||
<html> | <html> | ||
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8/17<br> | 8/17<br> | ||
| + | restriction emzyme fragmation<br> | ||
| + | K592024 B0015 C0051→Eco RI & SpeI<br> | ||
| + | I14144 R100 B0015 →XbaI & PstI<br> | ||
| + | EcoRI XbaI PstI →Promega<br> | ||
| + | SpeI→Takara<br> | ||
| + | <br> | ||
| + | 8/18<br> | ||
| + | transcription<br> | ||
| + | R0051 K592024 I14044 B0015 R0010<br> | ||
| + | C0051(psbic3)→E・coli-DH5X<br> | ||
| + | |||
| + | <br> | ||
| + | 8/18<br> | ||
| + | miniprep<br> | ||
| + | J04450<br> | ||
| + | colony PCR<br> | ||
| + | K592024 I14044 B0015 R0010 C0051 <br> | ||
| + | |||
| + | <br> | ||
| + | 8/23<br> | ||
| + | restriction emzyme fragmation<br> | ||
| + | |||
| + | <br> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <br> | ||
9/16<br> | 9/16<br> | ||
miniprep<br> | miniprep<br> | ||
restriction emzyme fragmation<br> | restriction emzyme fragmation<br> | ||
| + | |||
| + | |||
| + | <br> | ||
9/19<br> | 9/19<br> | ||
miniprep<br> | miniprep<br> | ||
| + | |||
| + | <br> | ||
9/21<br> | 9/21<br> | ||
miniprep<br> | miniprep<br> | ||
| + | |||
| + | <br> | ||
9/22<br> | 9/22<br> | ||
restriction emzyme fragmation<br> | restriction emzyme fragmation<br> | ||
miniprep<br> | miniprep<br> | ||
purify a gel<br> | purify a gel<br> | ||
| + | |||
| + | <br> | ||
9/23<br> | 9/23<br> | ||
miniprep<br> | miniprep<br> | ||
purify a gel<br> | purify a gel<br> | ||
| + | |||
| + | <br> | ||
9/24<br> | 9/24<br> | ||
restriction emzyme fragmation<br> | restriction emzyme fragmation<br> | ||
| + | |||
| + | <br> | ||
9/25<br> | 9/25<br> | ||
purify a gel<br> | purify a gel<br> | ||
ligation<br> | ligation<br> | ||
transformation<br> | transformation<br> | ||
| + | |||
| + | <br> | ||
9/26<br> | 9/26<br> | ||
miniprep<br> | miniprep<br> | ||
| + | |||
| + | <br> | ||
9/27<br> | 9/27<br> | ||
purify a gel<br> | purify a gel<br> | ||
restriction emzyme fragmation<br> | restriction emzyme fragmation<br> | ||
| + | |||
| + | |||
| + | <br> | ||
9/28<br> | 9/28<br> | ||
restriction emzyme fragmation<br> | restriction emzyme fragmation<br> | ||
| + | |||
| + | <br> | ||
9/30<br> | 9/30<br> | ||
restriction emzyme fragmation<br> | restriction emzyme fragmation<br> | ||
purify a gel<br> | purify a gel<br> | ||
| + | |||
| + | <br> | ||
10/1<br> | 10/1<br> | ||
ligation<br> | ligation<br> | ||
| Line 39: | Line 99: | ||
pre-culture<br> | pre-culture<br> | ||
purification of antisensence<br> | purification of antisensence<br> | ||
| + | |||
| + | <br> | ||
10/2<br> | 10/2<br> | ||
pre-culture<br> | pre-culture<br> | ||
miniprep<br> | miniprep<br> | ||
electrophoresis to confirm<br> | electrophoresis to confirm<br> | ||
| + | |||
| + | <br> | ||
10/3<br> | 10/3<br> | ||
miniprep<br> | miniprep<br> | ||
| Line 48: | Line 112: | ||
pre-culture<br> | pre-culture<br> | ||
purify a gel<br> | purify a gel<br> | ||
| + | |||
| + | <br> | ||
10/4<br> | 10/4<br> | ||
restriction emzyme fragmation<br> | restriction emzyme fragmation<br> | ||
| Line 53: | Line 119: | ||
miniprep<br> | miniprep<br> | ||
electrophoresis to confirm<br> | electrophoresis to confirm<br> | ||
| + | |||
| + | <br> | ||
10/5<br> | 10/5<br> | ||
ligation<br> | ligation<br> | ||
| Line 58: | Line 126: | ||
purify a gel<br> | purify a gel<br> | ||
restriction emzyme fragmation<br> | restriction emzyme fragmation<br> | ||
| + | |||
| + | <br> | ||
10/6<br> | 10/6<br> | ||
KIB…miniprep<br> | KIB…miniprep<br> | ||
electrophoresis to confirm<br> | electrophoresis to confirm<br> | ||
purify a gel<br> | purify a gel<br> | ||
| + | |||
| + | <br> | ||
10/7<br> | 10/7<br> | ||
restriction emzyme fragmation<br> | restriction emzyme fragmation<br> | ||
ligation<br> | ligation<br> | ||
transformation<br> | transformation<br> | ||
| + | |||
| + | <br> | ||
10/8<br> | 10/8<br> | ||
miniprep<br> | miniprep<br> | ||
purify a gel<br> | purify a gel<br> | ||
restriction emzyme fragmation<br> | restriction emzyme fragmation<br> | ||
| + | |||
| + | <br> | ||
10/9<br> | 10/9<br> | ||
pre-culture<br> | pre-culture<br> | ||
purify a gel<br> | purify a gel<br> | ||
| + | |||
| + | <br> | ||
10/10<br> | 10/10<br> | ||
ligation<br> | ligation<br> | ||
transformation<br> | transformation<br> | ||
| + | |||
| + | <br> | ||
10/11<br> | 10/11<br> | ||
pre-culture<br> | pre-culture<br> | ||
miniprep<br> | miniprep<br> | ||
| + | |||
| + | <br> | ||
10/12<br> | 10/12<br> | ||
purify a gel<br> | purify a gel<br> | ||
| + | |||
| + | <br> | ||
10/13<br> | 10/13<br> | ||
electrophoresis to confirm<br> | electrophoresis to confirm<br> | ||
| + | |||
| + | <br> | ||
10/14<br> | 10/14<br> | ||
restriction emzyme fragmation<br> | restriction emzyme fragmation<br> | ||
| + | |||
| + | |||
| + | <br> | ||
10/15<br> | 10/15<br> | ||
electrophoresis to confirm<br> | electrophoresis to confirm<br> | ||
restriction emzyme fragmation<br> | restriction emzyme fragmation<br> | ||
| + | |||
| + | |||
| + | <br> | ||
10/16<br> | 10/16<br> | ||
electrophoresis to confirm<br> | electrophoresis to confirm<br> | ||
Revision as of 12:49, 19 October 2016
8/17
restriction emzyme fragmation
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara
8/18
transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X
8/18
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051
8/23
restriction emzyme fragmation
9/16
miniprep
restriction emzyme fragmation
9/19
miniprep
9/21
miniprep
9/22
restriction emzyme fragmation
miniprep
purify a gel
9/23
miniprep
purify a gel
9/24
restriction emzyme fragmation
9/25
purify a gel
ligation
transformation
9/26
miniprep
9/27
purify a gel
restriction emzyme fragmation
9/28
restriction emzyme fragmation
9/30
restriction emzyme fragmation
purify a gel
10/1
ligation
transformation
pre-culture
purification of antisensence
10/2
pre-culture
miniprep
electrophoresis to confirm
10/3
miniprep
PCR
pre-culture
purify a gel
10/4
restriction emzyme fragmation
purify a gel
miniprep
electrophoresis to confirm
10/5
ligation
transformation
purify a gel
restriction emzyme fragmation
10/6
KIB…miniprep
electrophoresis to confirm
purify a gel
10/7
restriction emzyme fragmation
ligation
transformation
10/8
miniprep
purify a gel
restriction emzyme fragmation
10/9
pre-culture
purify a gel
10/10
ligation
transformation
10/11
pre-culture
miniprep
10/12
purify a gel
10/13
electrophoresis to confirm
10/14
restriction emzyme fragmation
10/15
electrophoresis to confirm
restriction emzyme fragmation
10/16
electrophoresis to confirm
