José Martín

1. Participation in the brainstorming and design of the project

2. All experiments related to the propionate module, including metabolic pathway design and plasmid construction

3. Participation in human practices in which the team was involved, including the elaboration of slides and posters, and science communication

4. Design the lab practices with students from the IES Martínez Montañés

5. Elaboration of notebook

6. Realization of the report of the GMOs survey

7. Elaboration of safety forms, and safety lab and safety project sections in the team wiki

8. Descriptions of the team basic parts and composite parts

Laura Claret

1. Participation in the brainstorming and design of the project

2. Participation in the brainstorming of microscopic prediction of biofilm growth modeling

3. All experiments related to alteration of c-diGMP in biofilm module, including plasmid construction, cloning of the genes, integration in P. putida genome and the physiological assays

4. Poster for the Giant Jamboree

5. Participation in all human practices in which the team was involved, including the elaboration of slides, posters and brochures

6. Participation in the lab practices with students from the IES Martínez Montañés

7. Elaboration of protocols and notebook

8. Realization of the surveys of our and other teams

9. Some public relations (updating Instagram)

10. Performing the migration of all constructions to pSB1C3 backbone

11. Off-voice in all the team videos

Álvaro Escobar

1. Participation in the brainstorming and design of the project

2. All experiments related to lapG and xylS2/Pm system (biofilm module), including plasmid construction, cloning of the genes, integration in P. putida genome and the physiological assays

3. Participation in all human practices in which the team was involved, including the elaboration of slides, posters and brochures

4. Design and participation in the lab practices with students from the IES Martínez Montañés

5. Elaboration of protocols and notebook

6. Realization of the surveys of our and others teams

7. Public relations (updating Twitter and Instagram pages)

8. Generate and design the wiki of our team

Israel León

1. Participation in some of the human practices in which the team was involved, such as the science fair, the lab practices with the students from IES Martínez Montañés and the science week

2. Development of the web site of the team

3. Did the team overview video

Gabriel Ruiz

1. Participation in the brainstorming and design of the project

2. All experiments related to glycerol module, including plasmid construction, cloning of the glpF gene, integration in the P. putida genome and physiological assays

3. Glycerol investigation (Integrated Human Practices)

4. Power point slides for the Giant Jamboree

5. Poster for the Giant Jamboree

6. Design of the banner for the Giant Jamboree

7. Participation in all human practices in which the team was involved, including the elaboration of slides, posters and brochures

8. Design and participation in the lab practices with students from the IES Martínez Montañés

9. Elaboration of protocols and notebook

10. Realization of the surveys of our and other teams

11. Some public relations (updating Facebook and twitter pages)

Francisco Javier Lobo

1. Participation in the brainstorming of microscopic prediction of biofilm growth modeling and population kinetics of biofilm bacteria modeling

2. Design and implementation of biological equations for microscopic prediction of biofilm growth modeling

3. Implementation of pysics equations under Alejandro Cuetos’ supervision for microscopic prediction of biofilm growth modeling

4. Development of population kinetics of biofilm forming bacteria modeling

5. Design and development of metabolic pathway software tool

6. Interaction with wet lab to apply the knowledege obtained with the simulations to the laboratory

7. Participation in human practices, such as Sevilla Science Fair

Maite González

1. Responsible of taking photos and videos of the Human Practices

2. Author of the reports of all human Practices

3. Creating and editing corporate videos

4. Participation in Human Practices, such as the Science Fair and meeting in Valencia with other iGEM groups

AKNOWLEDGEMENTS

We want to thank our instructors for their support and trust. Thanks to Rafael R. Daga for organizing all events, for being responsible of the bureaucracy (which is not little), and for helping us be a real team. Thanks to Fernando Govantes for teaching us all about P. putida and biofilms and for helping us in our experiments, always in a good mood. Thanks to Alejandro Cuetos for his help in both modeling tools and for giving so nice ideas for our project. Thanks to Norberto Díaz for his help in the construction of the web and YouTube pages. Without you, we wouldn’t have been able to live this amazing experience (and our power point would have been a disaster)!

Thanks to the Universidad Pablo de Olavide (Sevilla) and the Facultad de Ciencias Experimentales for allowing us participate in this and for helping us organizing most of Human Practices events. Specially, thanks to Bruno, C.viral, Zeiss and Biomol for trusting us and supporting us. We are very grateful.

We also would like to thank all people who helped us in the lab (including previous iGEM members) and our family and friends for encouraging us when our experiments didn’t work.