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Revision as of 13:08, 19 October 2016
Library Project
Overview
Motivation
As every house needs a cornerstone we need our binding protein library. The demand of easily accessible, fast adapting and specific binding proteins is ever-growing. To utilize the advantages of binding proteins for our project, a library, adapting the end-users (or more specific: our) needs, was designed by us.
When we were faced the challenge of designing a binding protein library, earlier this year, we glanced at existing iGEM projects and were surprised by not finding approaches in similar manner. Actually - our research proved our approach to be unprecedented in this competition. Introducing and providing one therefor contributes to the community as well. In this way, we hope to show the benefits to pave the way for a higher usage of libraries in iGEM.
When we were faced the challenge of designing a binding protein library, earlier this year, we glanced at existing iGEM projects and were surprised by not finding approaches in similar manner. Actually - our research proved our approach to be unprecedented in this competition. Introducing and providing one therefor contributes to the community as well. In this way, we hope to show the benefits to pave the way for a higher usage of libraries in iGEM.
Overview
To achieve generating the perfect binder an initial library is essential for our project. Thus, we set up the foundation to build our in vivo mutagenesis system on.
Our library layout is based on synthetic binding proteins as well as naturally occurring binding proteins. To start with, we chose two different scaffolds: Nanobodies and Monobodies. Each possessing advantages compared to conventional antibodies, making them suitable for our system. Read more about our used scaffolds.
Setting up a humongous library, containing all different kinds of variants, seemed tempting at first, but having a more defined and optimized library was the more rational choice. For doing so we involved only specific amino acids in the randomized regions of our binding proteins, to optimize binding of targets (Fellouse et al., 2004; Koide, 2009). Hence, we kept the theoretical variability to a maximum of about one billion (1,073,741, 824), just including estimatingly promising initial binders. See our Design and Construction for further insight.
Our library layout is based on synthetic binding proteins as well as naturally occurring binding proteins. To start with, we chose two different scaffolds: Nanobodies and Monobodies. Each possessing advantages compared to conventional antibodies, making them suitable for our system. Read more about our used scaffolds.
Setting up a humongous library, containing all different kinds of variants, seemed tempting at first, but having a more defined and optimized library was the more rational choice. For doing so we involved only specific amino acids in the randomized regions of our binding proteins, to optimize binding of targets (Fellouse et al., 2004; Koide, 2009). Hence, we kept the theoretical variability to a maximum of about one billion (1,073,741, 824), just including estimatingly promising initial binders. See our Design and Construction for further insight.
![](https://static.igem.org/mediawiki/2016/c/c9/Bielefeld_CeBiTec_2016_10_18_LIB_project_overview_library.png)
Literature
- Fellouse, Frederic A., 2004. Synthetic Antibodies from a Four-Amino-Acid Code: A Dominant Role for Tyrosine in Antigen Recognition. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101, 34, 12467.
- Koide, S 2009. The Importance of Being Tyrosine: Lessons in Molecular Recognition from Minimalist Synthetic Binding Proteins. ACS CHEMICAL BIOLOGY, 2009, 4, 5, 325.