Difference between revisions of "Team:FAFU-CHINA/Basic Part"
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| − | <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:":,sans-serif;color:#1E4E79">Cry11Aa4+Extended2A+EGFP-pChlamy3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | + | = <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:":,sans-serif;color:#1E4E79">Cry11Aa4+Extended2A+EGFP-pChlamy3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> = |
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Revision as of 14:43, 19 October 2016
Contents
- 1 Cry4Aa4+ExtendedFMDV 2A-pChlamy_3
- 2 Cry4Aa4+ExtendedFMDV 2A-pChlamy_3
- 3 Cyt1-pChlamy_3
- 4 Cyt2-pChlamy_3
- 5 EGFP-pChlamy_3
- 6 Cry4Aa4+Extended2A+Cyt2-pChlamy3
- 7 Cry4Aa4+Extended2A+Cyt1-pChlamy3
- 8 Cry10Aa4+Extended2A+Cyt2-pChlamy3
- 9 Cry10Aa4+Extended2A+Cyt2-pChlamy3
- 10 Cry10Aa4+Extended2A+Cyt1-pChlamy3
- 11 Cry11Aa4+Extended2A+Cyt2-pChlamy3
- 12 Cry11Aa4+Extended2A+Cyt1-pChlamy3
- 13 Cry11Aa4+Extended2A+EGFP-pChlamy3
Cry4Aa4+ExtendedFMDV 2A-pChlamy_3
| Gene Name | Cry4Aa4+Extended FMDV 2A |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in DH5α |
| Gene Length | 3673bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
| Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA |
Cry4Aa4+ExtendedFMDV 2A-pChlamy_3
| Gene Name | Cry10Aa4+Extended FMDV 2A |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in Top10 |
| Gene Length | 2158bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
| Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA |
Cry11Aa4+Extended FMDV 2A-pChlamy_3
| Gene Name | Cry11Aa4+ Extended FMDV 2A |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in JM108 |
| Gene Length | 2071bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
| Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cyt1-pChlamy_3
| Gene Name | Cyt1 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in JM108 |
| Gene Length | 762bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cyt2-pChlamy_3
| Gene Name | Cyt1 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in JM108 |
| Gene Length | 801bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results | |||
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass | |||
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass | |||
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass | |||
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass | |||
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass | |||
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass | |||
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass | |||
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
| |||
EGFP-pChlamy_3
| Gene Name | 06-EGFP（TAA） |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in Top10 |
| Gene Length | 732bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry4Aa4+Extended2A+Cyt2-pChlamy3
| Gene Name | Cry4Aa4+Extended2A+Cyt2 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in Top10 |
| Gene Length | 4461bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and BamHI
Lane 2: Plasmid DNA
|
Cry4Aa4+Extended2A+Cyt1-pChlamy3
| Gene Name | Cry4Aa4+Extended2A+Cyt1-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in JM108 |
| Gene Length | 4422bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results | |||
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass | |||
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass | |||
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass | |||
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass | |||
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass | |||
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass | |||
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass | |||
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by BamHI and KpnI
Lane 2: Plasmid DNA
|
| |||
Cry10Aa4+Extended2A+Cyt2-pChlamy3
| Gene Name | Cry10Aa4+Extended2A+Cyt2-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in DH5α |
| Gene Length | 2946bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry10Aa4+Extended2A+Cyt2-pChlamy3
| Gene Name | Cry10Aa4+Extended2A+Cyt2-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in DH5α |
| Gene Length | 2946bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry10Aa4+Extended2A+Cyt1-pChlamy3
| Gene Name | Cry10Aa4+Extended2A+Cyt1-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in DH5α |
| Gene Length | 2907bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results | |||
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass | |||
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass | |||
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass | |||
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass | |||
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass | |||
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass | |||
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass | |||
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
| |||
Cry11Aa4+Extended2A+Cyt2-pChlamy3
| Gene Name | Cry11Aa4+Extended2A+Cyt2-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in Top10 |
| Gene Length | 2859bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry11Aa4+Extended2A+Cyt1-pChlamy3
| Gene Name | Cry11Aa4+Extended2A+Cyt1-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in JM108 |
| Gene Length | 2820bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry11Aa4+Extended2A+EGFP-pChlamy3
| Gene Name | Cry11Aa4+Extended2A+EGFP-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in JM108 |
| Gene Length | 2790bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
