Difference between revisions of "Team:BGU ISRAEL/Parts"

 
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                             <ul class="dropdown-menu submenubgu">
 
                             <ul class="dropdown-menu submenubgu">
 
                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Parts#Overview">Overview</a></li>
 
                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Parts#Overview">Overview</a></li>
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                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Basic_Part">Basic Part</a></li>
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                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Basic_Part">Basic Parts</a></li>
 
                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/ImprovedParts">Improved Parts</a></li>
 
                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/ImprovedParts">Improved Parts</a></li>
 
                             </ul>
 
                             </ul>
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                             <ul class="dropdown-menu submenubgu">
 
                             <ul class="dropdown-menu submenubgu">
 
                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Integrated_Practices">Integrated Practices</a></li>
 
                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Integrated_Practices">Integrated Practices</a></li>
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                                <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Engagement">Public Engagement</a></li>
 
 
                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/PlasticArt">Plastic Art</a></li>
 
                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/PlasticArt">Plastic Art</a></li>
 
                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/EthicsAndSafety">Ethics & Safety</a></li>
 
                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/EthicsAndSafety">Ethics & Safety</a></li>
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                             <ul class="dropdown-menu submenubgu">
 
                             <ul class="dropdown-menu submenubgu">
 
                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Entrepreneurship">Entrepreneurship</a></li>
 
                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Entrepreneurship">Entrepreneurship</a></li>
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                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Model">Model</a></li>
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                                 <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Measurements">Measurements</a></li>
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                                <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Proof">Proof Of Concept</a></li>
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                                <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Demonstrate">Demonstrate</a></li>
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                                <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/HP/Silver">HP - Silver</a></li>
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                                <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/HP/Gold">HP - Gold</a></li>
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                                <li><a href="https://2016.igem.org/Team:BGU_ISRAEL/Engagement">Engagements</a></li>
 
                             </ul>
 
                             </ul>
 
                         </li>
 
                         </li>
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                             <div class="col-lg-8">
 
                             <div class="col-lg-8">
 
                                 <p class="bigAssHeader">Overview</p>
 
                                 <p class="bigAssHeader">Overview</p>
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                                <hr>
 
 
                                 <div class="partOverviewText">
 
                                 <div class="partOverviewText">
 
                                     <p>
 
                                     <p>
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                                     <ol>
 
                                         <li>
 
                                         <li>
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                                             The chassis for our project is based on the <i>Pseudomonas putida</i> bacterium, for its ability to utilize aromatic molecules, mainly focusing on protocatechuate. We have decided to contribute the the iGEM part repository by submitting the genes encoding the enzymes participating in the protocatechuate degradation pathway, in which Protocatechuate, a toxic molecule for most bacteria, is converted to 3-oxoadipate. One of the genes, pcaB, had 4 PstI restriction cut sites and we did not have enough time to introduce silent mutations to the sequence, hence we did not clone and submit it.  
+
                                             The chassis for our project is based on the <i>Pseudomonas putida</i> bacterium, for its ability to utilize aromatic molecules, mainly focusing on protocatechuate. We have decided to contribute the the iGEM part repository by submitting the genes encoding the enzymes participating in the protocatechuate degradation pathway, in which Protocatechuate, a toxic molecule for most bacteria, is converted to 3-oxoadipate. One of the genes, pcaB, had 4 PstI restriction cut sites and we did not have enough time to introduce silent mutations to the sequence, hence we did not clone and submit it. All 4 other genes were cloned into the pSB1C3 vector and submitted. 
 
                                         </li>
 
                                         </li>
 
                                         <li>
 
                                         <li>
 
                                             Another part of our project involves engineering of the LC-Cutinase protein. We have chosen the LC-Cutinase protein as a target for rational mutagenesis for its PET degrading activity, and using the PROSS algorithm produced 4 mutants.  
 
                                             Another part of our project involves engineering of the LC-Cutinase protein. We have chosen the LC-Cutinase protein as a target for rational mutagenesis for its PET degrading activity, and using the PROSS algorithm produced 4 mutants.  
 
                                             We have also designed a codon optimized version of the W.T. protein.  
 
                                             We have also designed a codon optimized version of the W.T. protein.  
 +
                                            We have cloned and submitted all 5 LC-Cutinase variants.
 
                                         </li>
 
                                         </li>
 
                                     </ol>
 
                                     </ol>
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                                 <img class="partOverviewPic" src="https://static.igem.org/mediawiki/2016/b/b6/BioBrick_overviewBGU.png">
 
                                 <img class="partOverviewPic" src="https://static.igem.org/mediawiki/2016/b/b6/BioBrick_overviewBGU.png">
 
                                 <br>
 
                                 <br>
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                                 <a href="https://2016.igem.org/Team:BGU_ISRAEL/Basic_Part" class="btn btn-primary infobtn" role="button">Come see our BioBricks</a>
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                                 <div class='row'>
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                                    <div class='col-lg-1'></div>
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                                    <div class='col-lg-5'>
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                                        <a href="https://2016.igem.org/Team:BGU_ISRAEL/Basic_Part" class="btn btn-primary infobtn" role="button">Basic Parts</a>
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                                    </div>
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                                    <div class='col-lg-5'>
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                                        <a href="https://2016.igem.org/Team:BGU_ISRAEL/ImprovedParts" class="btn btn-primary infobtn" role="button">Improved Parts</a>
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                                    </div>
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                                </div>
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                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
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             </div>
 
             </div>
 
         </div>
 
         </div>
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             <a href="#" class="back-to-top">
 
             <a href="#" class="back-to-top">
 
             <img src='https://static.igem.org/mediawiki/2016/5/52/Arrow_for_site.png'>
 
             <img src='https://static.igem.org/mediawiki/2016/5/52/Arrow_for_site.png'>
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             });
 
             });
 
         </script>
 
         </script>
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        <div class="container-fluid">
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             <div class="row">
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                 <footer class="mainfooter">
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<div class="container-fluid">
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             <div class="row footerColor">
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                 <div class="col-lg-2"></div>
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                <div class="col-lg-4 text-center" >
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                    <h4>Address:</h4>
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                    <p>
 +
                        Ben-Gurion University of the Negev<br>
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                        Ben Gurion 1, Beer Sheva 8410501, Israel
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                    </p>
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                    <h5>
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                        <b><u>Mail:</u></b> igembgu2016@gmail.com
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                    </h5>
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                </div>
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                <div class="col-lg-4 text-center">
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                    <h4>Connect With Us!</h4>
 
                     <div class="row">
 
                     <div class="row">
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                         <div class="col-sm-4 text-center" >
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                         <div class="col-lg-3"></div>
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                            <h4>Address:</h4>
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                        <div class="col-lg-6">
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                            <p>
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                             <div class="row">
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                                <b>
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                                 <div class="col-lg-4 socialWrapper">
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                                    Ben-Gurion University of the Negev<br>
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                                     <a href="https://www.facebook.com/iGEMBGU/?fref=ts" target="_blank"><img class="socialMediaIcon" src="https://static.igem.org/mediawiki/2016/7/7d/FacebookFlatIcon.png" alt="facebook"></a>
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                                    Ben Gurion 1, Beer Sheva 8410501, Israel
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                                 </div>
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                                </b>
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                                 <div class="col-lg-4 socialWrapper">
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                             </p>
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                                     <a href=""><img class="socialMediaIcon" src="https://static.igem.org/mediawiki/2016/1/15/InstagramFlatIcon.png" alt="instagram"></a>
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                            <h5>
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                                 </div>
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                                 <b><u>Mail:</u> igembgu2016@gmail.com</b>
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                                 <div class="col-lg-4 socialWrapper">
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                            </h5>
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                                     <a href="https://twitter.com/igembgu2016" target="_blank"><img class="socialMediaIcon" src="https://static.igem.org/mediawiki/2016/3/34/TwitterFlatIcon.png" alt="twitter"></a>
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                                 </div>
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                        <div class="col-sm-4 text-center">
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                             </div>
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                            <h4>Connect With Us!</h4>
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                            <ul>
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                                <li>
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                                     <a href="https://www.facebook.com/iGEMBGU/?fref=ts" target="_blank"><img class="socialMediaIcon" src="media/flat-social-media-icons/facebook.png" alt="facebook"></a>
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                                     <a ><img class="socialMediaIcon" src="media/flat-social-media-icons/instagram.png" alt="instagram"></a>
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                                     <a href="https://twitter.com/igembgu2016" target="_blank"><img class="socialMediaIcon" src="media/flat-social-media-icons/twitter.png" alt="twitter"></a>
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                                 </li>
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                        <div class="col-sm-4 text-center">
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                            <h4>CopyRights</h4>
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                         </div>
 
                         </div>
 
                     </div>
 
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                 </div>
 
             </div>
 
             </div>
 
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Latest revision as of 14:54, 19 October 2016

PlastiCure

Overview

We have decided to submit 9 new BioBricks this year, which are separated into 2 categories:

  1. The chassis for our project is based on the Pseudomonas putida bacterium, for its ability to utilize aromatic molecules, mainly focusing on protocatechuate. We have decided to contribute the the iGEM part repository by submitting the genes encoding the enzymes participating in the protocatechuate degradation pathway, in which Protocatechuate, a toxic molecule for most bacteria, is converted to 3-oxoadipate. One of the genes, pcaB, had 4 PstI restriction cut sites and we did not have enough time to introduce silent mutations to the sequence, hence we did not clone and submit it. All 4 other genes were cloned into the pSB1C3 vector and submitted.
  2. Another part of our project involves engineering of the LC-Cutinase protein. We have chosen the LC-Cutinase protein as a target for rational mutagenesis for its PET degrading activity, and using the PROSS algorithm produced 4 mutants. We have also designed a codon optimized version of the W.T. protein. We have cloned and submitted all 5 LC-Cutinase variants.

Basic Parts
Improved Parts

Address:

Ben-Gurion University of the Negev
Ben Gurion 1, Beer Sheva 8410501, Israel

Mail: igembgu2016@gmail.com

Connect With Us!