Difference between revisions of "Team:Nagahama/Proof"

 
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{{Nagahama}}
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:We did an experiment that the effect of suppressing the mold by geraniol and farnesol to improve the performance of FLAVORATOR. 
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[[File:Nagahama_POC実験.jpg|700px|thumb|none|''Fig.Comparison of the effect of suppressing the mold by geraniol and farnesol''
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<br>
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Fig1:Comparison of food preservation effect of geraniol ,farnesol and ethanol.
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(A)geraniol(100μl of geraniol solution was soaked on the cotton)(B)farnesol(in the same way)(C)ethanol (in the same way)These boxes were kept for 5 days at room temperature.
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<h3>Protocols</h3>
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'''Fig.1 Comparison of food preservation effect of geraniol ,farnesol and ethanol'''
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:Coating the suspension of mold in bread, it was easier to mold.Put the bread and cotton soaked geraniol(100µl) in a box of plastic, were separated so as not to directly touch.Farnesol and ethanol were also carried out the same experiment.
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This result,farnesol was found that the highest antibacterial activity.
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iGEM teams are great at making things work! We value teams not only doing an incredible job with theoretical models and experiments, but also in taking the first steps to make their project real.
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What should we do for our proof of concept?
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You can assemble a device from BioBricks and show it works. You could build some equipment if you're competing for the hardware award. You can create a working model of your software for the software award. Please note that this not an exhaustive list of activities you can do to fulfill the gold medal criterion. As always, your aim is to impress the judges!
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<div class="menu_wrapper" >
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<div class="collapsable_menu_control"> MENU ▤ </div>
 +
 
 +
<ul id="accordion" class="accordion">
 +
 
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama">HOME </a> </li>
 +
 
 +
<li class="menu_item"> <div class="icon plus"></div> PROJECT 
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<ul class="submenu">
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<li> <a href="https://2016.igem.org/Team:Nagahama/Introduction">  Introduction </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Result and Discussion"> Result and Discussion </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Future"> Future </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Protocol"> Protocol </a></li>
 +
                    </ul>
 +
                </li>
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<li class="menu_item"> <div class="icon plus"></div> BioBricks 
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<ul class="submenu">
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<li> <a href="https://2016.igem.org/Team:Nagahama/Our all BioBricks">  Our all BioBricks </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/BioBricks for medal"> BioBricks for medal </a></li>
 +
                    </ul>
 +
                </li>
 +
 
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama/Safety"> Safety </a> </li>
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<li class="menu_item"> <div class="icon plus"></div> Attributions
 +
<ul class="submenu">
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<li> <a href="https://2016.igem.org/Team:Nagahama/Team">  Team</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Attributions"> Attributions </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Sponsors"> Sponsors</a></li>
 +
 
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                    </ul>
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                </li>
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<li class="menu_item"> <div class="icon plus"></div> Collaborations
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<ul class="submenu">
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<li> <a href="https://2016.igem.org/Team:Nagahama/Collaborations">  Collaborations</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/The Annual Meeting Of The Genetics Society Of Japan"> The Annual Meeting Of The Genetics Society Of Japan </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Support Gifu"> Support Gifu</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/virginia">  Virginia</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Paris-Saclay">  Paris-Saclay</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Munich United"> Munich United</a></li>
 +
 
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                    </ul>
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                </li>
 +
 
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<li class="menu_item"> <div class="icon plus"></div> Human Practice
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<ul class="submenu">
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<li> <a href="https://2016.igem.org/Team:Nagahama/Human Practice"> Human Practice </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Local"> Contacting Local</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Japan"> Contacting Japan</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Intgrated Human Practice"> Intgrated Human Practice</a></li>
 +
 
 +
 
 +
 
 +
                    </ul>
 +
                </li>
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama/Proof of concept"> Proof of concept </a> </li>
 +
 
 +
 
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama/Demonstrate your work"> Demonstrate your work </a> </li>
 +
 
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              　　　　　　　</ul>
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　　        </li>
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<h1 id="team_name">  </h1>
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</script>
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</html>
 +
ＭarA<br>
 +
This device makes Escherichia coli have the resistance of farnesol.
 +
Actually, we confirmed resistance of farnesol by MarA.
 +
=='''Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates. '''==
 +
[[File:E.colimarA result .jpg|800px|thumb|center|Fig.7:Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.
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<br>
 +
 
 +
''E.coli'' JM109 and ''E.coli'' JM109 (marA) were dropped on LBGMg agar plates in serial ten-fold dilutions (10⁻¹～〖10〗^(-6)), overlaid with 30 % (v/v)　farnesol hexane solution (farnesol solution) and incubated at 30°C for 24 h. This figure shows that ''E.coli'' JM109 (marA) cells that overexpress the marA product better than the control ''E.coli'' JM109 wild type cells survived plates overloyed by 30 % farnesol solution.
 +
]]
 +
From the results, the device we built performs its desired function.

Latest revision as of 15:43, 19 October 2016

MENU ▤
　　

ＭarA
This device makes Escherichia coli have the resistance of farnesol. Actually, we confirmed resistance of farnesol by MarA.

Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.

Fig.7:Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.
E.coli JM109 and E.coli JM109 (marA) were dropped on LBGMg agar plates in serial ten-fold dilutions (10⁻¹～〖10〗^(-6)), overlaid with 30 % (v/v)　farnesol hexane solution (farnesol solution) and incubated at 30°C for 24 h. This figure shows that E.coli JM109 (marA) cells that overexpress the marA product better than the control E.coli JM109 wild type cells survived plates overloyed by 30 % farnesol solution.

From the results, the device we built performs its desired function.