Difference between revisions of "Team:Nagahama/Proof"

 
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{{Nagahama}}
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<html>
  
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[[File:Nagahama_POC実験.jpg|700px]]
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<style>
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 +
/********************************* DEFAULT WIKI SETTINGS  ********************************/
 +
 
 +
#sideMenu, #top_title {display:none;}
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#content { padding:0px; width:1000px; margin-top:-7px; margin-left:0px;}
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body {background-color:white; }
 +
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 +
 
 +
/********************************* MENU ********************************/
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/* Wrapper for the menu */
 +
.menu_wrapper {
 +
width:150px;
 +
height:100vh;
 +
position:fixed;
 +
padding:0px;
 +
float:left;
 +
background-color:#f2f2f2;
 +
text-align:left;
 +
}
 +
 
 +
/* styling for the menu items */
 +
.menu_item {
 +
width:100%;
 +
margin:-2px 0px 0px -20px;
 +
padding: 10px 10px; 
 +
border-bottom: 1px solid #d3d3d3;
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font-weight:bold;
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color:#000000;
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cursor: pointer;
 +
}
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 +
/* when hovering on a menu item */
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.menu_item:hover {
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color:#000000;
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background-color: #72c9b6;
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}
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/* decoration icon for the menu buttons*/
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.icon {
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float:right; 
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font-size:16px;
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font-weight:bold;
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}
 +
 +
/* this is the icon for when the content is collapsed */
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.plus::before {
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content: "+";
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}
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/* this is the icon for when the content is expanded */
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.less::before {
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content: "–";
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}
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 +
/* styling for the ul that creates the buttons */
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ul.menu_items {
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width:100%;
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margin-left:0px;
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padding:0px;
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list-style: none;
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}
 +
 
 +
/* styling for the li that are the menu items */
 +
.menu_items li {
 +
width:90%;
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margin-top:-2px;
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padding: 15px 0px 15px 15px ;
 +
display:block;
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border-bottom: 1px solid #d3d3d3; 
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text-align:left;
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font-weight:bold;
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text-decoration:none;
 +
color:#000000;
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list-style-type:none;
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cursor:pointer;
 +
-webkit-transition: all 0.4s ease;
 +
-moz-transition: all 0.4s ease;
 +
-ms-transition: all 0.4s ease;
 +
-o-transition: all 0.4s ease; transition: all 0.4s ease; 
 +
}
 +
 
 +
.menu_item a {
 +
width: 100%;
 +
margin-left: -20px;
 +
padding: 11px 90px 12px 20px;
 +
text-decoration: none;
 +
color:black;
 +
}
 +
 
 +
/* When hovering on a menu item */
 +
.menu_items li:hover {
 +
background-color:#72c9b6;
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color: #000000;
 +
}
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 +
/* styling for the submenus */
 +
.submenu {
 +
width:100%;
 +
display: none; 
 +
font-weight:bold;
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cursor:pointer;
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}
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 +
/* moving the margin for the submenu ul list */
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ul.submenu {
 +
width: 100%;
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margin: 10px 0px -11px 0px;
 +
list-style: none;
 +
}
 +
 
 +
/*styling for the submenu buttons */
 +
.submenu li {
 +
width: 100%;
 +
margin-left: 10px;
 +
margin-bottom: 0px;
 +
}
 +
 
 +
 
 +
/* hover state for the submenu buttons */
 +
.submenu li a {
 +
width: 100%;
 +
padding: 5px 10px;
 +
display: inline-block;
 +
border-bottom: 1px solid #d3d3d3;
 +
background-color:white;
 +
text-decoration:none;
 +
color:#000000;
 +
}
 +
 
 +
 
 +
 
 +
.submenu li a:hover  {
 +
background-color:#000000;
 +
color: #72c9b6;
 +
}
 +
 
 +
 
 +
/* When the screen is smaller than 680px, the menu has the option to hide/show - this button controls that */
 +
.collapsable_menu_control {
 +
width:100%;
 +
padding: 15px 0px;
 +
display:none;
 +
background-color:#000000;
 +
text-align:center; 
 +
font-weight:bold;
 +
color:#72c9b6;
 +
cursor:pointer;
 +
-webkit-transition: all 0.4s ease;
 +
-moz-transition: all 0.4s ease;
 +
-ms-transition: all 0.4s ease;
 +
-o-transition: all 0.4s ease;
 +
transition: all 0.4s ease;
 +
}
 +
 
 +
/* when hovering on that button */
 +
.collapsable_menu_control:hover {
 +
background-color: #72c9b6;
 +
color:#000000; 
 +
}
 +
 
 +
/********************************* CONTENT OF THE PAGE ********************************/
 +
 
 +
/* Wrapper for the content */
 +
.content_wrapper {
 +
width: 85%;
 +
margin-left:150px;
 +
padding:10px 0px;
 +
float:left;
 +
background-color:white;
 +
}
 +
 
 +
/*LAYOUT */
 +
.column  {
 +
padding: 10px 0px;
 +
float:left;
 +
background-color:white;
 +
}
 +
 
 +
.full_size {
 +
width:100%;
 +
}
 +
 
 +
.full_size img {
 +
padding: 10px 15px;
 +
width:96.5%;
 +
}
 +
 
 +
.half_size {
 +
width: 50%;
 +
}
 +
.half_size img {
 +
padding: 10px 15px;
 +
width: 93%;
 +
}
 +
 
 +
 
 +
.clear {
 +
clear:both;
 +
}
 +
 
 +
.highlight {
 +
width: 90%;
 +
margin: auto;
 +
padding: 15px 5px;
 +
background-color: #f2f2f2;
 +
}
 +
 
 +
. {
 +
border: 4px solid #72c9b6;
 +
display: block;
 +
margin: 5px 15px;
 +
width: 95%;
 +
font-weight:bold;
 +
}
 +
 
 +
 
 +
/*STYLING */
 +
 
 +
/* styling for the titles */
 +
.content_wrapper h1, .content_wrapper h2 {
 +
padding:5px 15px;
 +
border-bottom:0px;
 +
color:#72c9b6;
 +
 
 +
}
 +
.content_wrapper h3, .content_wrapper h4, .content_wrapper h5, .content_wrapper h6 {
 +
padding:5px 15px;
 +
border-bottom:0px;
 +
color: #000000; 
 +
}
 +
 
 +
 
 +
/* font and text */
 +
.content_wrapper p {
 +
padding:0px 15px;
 +
font-size: 13px;
 +
font-family:Tahoma, Geneva, sans-serif;
 +
}
 +
 
 +
/* Links */
 +
.content_wrapper a {
 +
font-weight: bold;
 +
text-decoration: underline;
 +
text-decoration-color:#72c9b6;
 +
color: #72c9b6;
 +
-webkit-transition: all 0.4s ease;
 +
-moz-transition: all 0.4s ease;
 +
-ms-transition: all 0.4s ease;
 +
-o-transition: all 0.4s ease;
 +
transition: all 0.4s ease;
 +
}
 +
 
 +
/* hover for the links */
 +
.content_wrapper a:hover {
 +
text-decoration:none;
 +
color:#000000;
 +
}
 +
 
 +
/* non numbered lists */
 +
.content_wrapper ul {
 +
padding:0px 20px;
 +
font-size: 13px;
 +
font-family:Tahoma, Geneva, sans-serif;
 +
}
 +
 
 +
/* numbered lists */
 +
.content_wrapper ol {
 +
padding:0px;
 +
font-size: 13px;
 +
font-family:Tahoma, Geneva, sans-serif;
 +
}
 +
 
 +
/* Table */
 +
.content_wrapper table {
 +
width: 97%;
 +
margin:15px 10px;
 +
border: 1px solid #d3d3d3;
 +
border-collapse: collapse;
 +
}
 +
 
 +
/* table cells */
 +
.content_wrapper  td {
 +
padding: 10px;
 +
vertical-align: text-top;
 +
border: 1px solid #d3d3d3;
 +
border-collapse: collapse;
 +
}
 +
 
 +
/* table headers */
 +
.content_wrapper th {
 +
padding: 10px;
 +
vertical-align: text-top;
 +
border: 1px solid #d3d3d3;
 +
border-collapse: collapse;
 +
background-color:#f2f2f2;
 +
}
 +
 
 +
/* Button class */
 +
.button_click {
 +
margin: 10px auto;
 +
padding: 15px; width:12%; 
 +
text-align:center;
 +
font-weight:bold;
 +
background-color: #72c9b6;
 +
cursor:pointer; 
 +
-webkit-transition: all 0.4s ease;
 +
-moz-transition: all 0.4s ease;
 +
-ms-transition: all 0.4s ease;
 +
-o-transition: all 0.4s ease;
 +
transition: all 0.4s ease;
 +
}
 +
 
 +
/* button class on hover */
 +
.button_click:hover { 
 +
background-color:#000000;
 +
color:#72c9b6;
 +
}
 +
 
 +
/********************************* RESPONSIVE STYLING ********************************/
 +
 
 +
/* IF THE SCREEN IS LESS THAN 1000PX */
 +
 
 +
@media only screen and (max-width: 1000px) {
 +
 
 +
#content {width:100%; }
 +
.menu_wrapper {width:15%;}
 +
.content_wrapper {margin-left: 15%;}
 +
.menu_item {display:block;}
 +
.icon {display:none;}
 +
.highlight {padding:10px 0px;}
 +
}
 +
 
 +
 
 +
/* IF THE SCREEN IS LESS THAN 680PX */
 +
 
 +
@media only screen and (max-width: 680px) {
 +
.collapsable_menu_control { display:block;}
 +
.menu_item {display:none;}
 +
.menu_wrapper { width:100%; height: 15%; position:relative;}
 +
.content_wrapper {width:100%; margin-left:0px;}
 +
.column.half_size {width:100%; }
 +
.column img { width: 100%; padding: 5px 0px;}
 +
.icon {display:block;}
 +
.highlight {padding:15px 5px;}
 +
}
 +
 
 +
</style>
 +
 
 +
 
 +
 
 +
 
 +
<!--- THIS IS WHERE THE HTML BEGINS --->
 +
 
 +
 
 +
<!-- This tells the browser that your page is responsive -->
 +
 
 +
<head>
 +
<meta name="viewport" content="width=device-width, initial-scale=1">
 +
</head>
 +
 
 +
 
 +
 
 +
 
 +
<div class="menu_wrapper" >
 +
 
 +
 
 +
 
 +
 
 +
<div class="collapsable_menu_control"> MENU ▤ </div>
 +
 
 +
<ul id="accordion" class="accordion">
 +
 
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama">HOME </a> </li>
 +
 
 +
<li class="menu_item"> <div class="icon plus"></div> PROJECT 
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Introduction">  Introduction </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Result and Discussion"> Result and Discussion </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Future"> Future </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Protocol"> Protocol </a></li>
 +
                    </ul>
 +
                </li>
 +
 
 +
<li class="menu_item"> <div class="icon plus"></div> BioBricks 
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Our all BioBricks">  Our all BioBricks </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/BioBricks for medal"> BioBricks for medal </a></li>
 +
                    </ul>
 +
                </li>
 +
 
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama/Safety"> Safety </a> </li>
 +
 
 +
 
 +
<li class="menu_item"> <div class="icon plus"></div> Attributions
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Team">  Team</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Attributions"> Attributions </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Sponsors"> Sponsors</a></li>
 +
 
 +
                    </ul>
 +
                </li>
 +
 
 +
 
 +
<li class="menu_item"> <div class="icon plus"></div> Collaborations
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Collaborations">  Collaborations</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/The Annual Meeting Of The Genetics Society Of Japan"> The Annual Meeting Of The Genetics Society Of Japan </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Support Gifu"> Support Gifu</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/virginia">  Virginia</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Paris-Saclay">  Paris-Saclay</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Munich United"> Munich United</a></li>
 +
 
 +
 
 +
 
 +
 
 +
                    </ul>
 +
                </li>
 +
 
 +
<li class="menu_item"> <div class="icon plus"></div> Human Practice
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Human Practice"> Human Practice </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Local"> Contacting Local</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Japan"> Contacting Japan</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Intgrated Human Practice"> Intgrated Human Practice</a></li>
 +
 
 +
 
 +
 
 +
                    </ul>
 +
                </li>
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama/Proof of concept"> Proof of concept </a> </li>
 +
 
 +
 
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama/Demonstrate your work"> Demonstrate your work </a> </li>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 +
 
 +
 
 +
              　　　　　　　</ul>
 +
　　        </li>
 +
 
 +
 
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<div class="content_wrapper">
 +
 
 +
 
 +
 
 +
<h1 id="team_name">  </h1>
 +
<h4 id="page_name">  </h4>
 +
 
 +
 
 +
 
 +
 
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 +
 
 +
 
 +
 
 +
 
 +
<script>
 +
 
 +
// This is the jquery part of your template.  Try not modify any of this code since it makes your menu work.
 +
 
 +
$(document).ready(function() {
 +
 
 +
$("#HQ_page").attr('id','');
 +
 +
 
 +
if ( wgPageName.substring( 0,  8) == "Template")  {  // if the page is a template it displays the full name in a single line
 +
$("#team_name").html( wgPageName );
 +
}
 +
 
 +
else if (  ( (wgPageName.match(/\//g) || []).length ) == 0 ) {  // if it is the home page , just print the team's name
 +
$("#team_name").html( wgPageName.substring( 5, wgPageName.length ) );
 +
}
 +
 
 +
else {
 +
// this adds the team's name as an h1
 +
$("#team_name").html( wgPageName.substring( 5 , wgPageName.indexOf("/")  ) );
 +
 
 +
// this adds the page's title as an h4
 +
$("#page_name").html (    ( wgPageName.substring( wgPageName.indexOf("/") + 1, wgPageName.length ) ).replace( /\/|_/g , " ")  );
 +
}
 +
 
 +
 
 +
 
 +
 
 +
$('#accordion').find('.menu_item').click(function(){
 +
 
 +
//Expand or collapse this panel
 +
submenu = $(this).find('.submenu');
 +
submenu.toggle();
 +
 
 +
icon = $(this).find('.icon');
 +
 
 +
if ( !$( submenu ).is(':visible') ) {
 +
icon.removeClass("less").addClass("plus");
 +
}
 +
else {
 +
icon.removeClass("plus").addClass("less");
 +
}
 +
 
 +
//Hide the other panels
 +
$(".submenu").not(submenu).hide();
 +
$(".icon").not(icon).removeClass("less").addClass("plus");
 +
});
 +
 
 +
 
 +
$(".collapsable_menu_control").click(function() {
 +
$(".menu_item").toggle();
 +
});
 +
 
 +
$( window ).resize(function() {
 +
$(".menu_item").show();
 +
});
 +
 
 +
 
 +
});
 +
 
 +
 
 +
 
 +
 
 +
</script>
 +
 
 +
 
 +
</html>
 +
ＭarA<br>
 +
This device makes Escherichia coli have the resistance of farnesol.
 +
Actually, we confirmed resistance of farnesol by MarA.
 +
=='''Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates. '''==
 +
[[File:E.colimarA result .jpg|800px|thumb|center|Fig.7:Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.
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<br>
 +
 
 +
''E.coli'' JM109 and ''E.coli'' JM109 (marA) were dropped on LBGMg agar plates in serial ten-fold dilutions (10⁻¹～〖10〗^(-6)), overlaid with 30 % (v/v)　farnesol hexane solution (farnesol solution) and incubated at 30°C for 24 h. This figure shows that ''E.coli'' JM109 (marA) cells that overexpress the marA product better than the control ''E.coli'' JM109 wild type cells survived plates overloyed by 30 % farnesol solution.
 +
]]
 +
From the results, the device we built performs its desired function.

Latest revision as of 15:43, 19 October 2016

MENU ▤
　　

ＭarA
This device makes Escherichia coli have the resistance of farnesol. Actually, we confirmed resistance of farnesol by MarA.

Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.

Fig.7:Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.
E.coli JM109 and E.coli JM109 (marA) were dropped on LBGMg agar plates in serial ten-fold dilutions (10⁻¹～〖10〗^(-6)), overlaid with 30 % (v/v)　farnesol hexane solution (farnesol solution) and incubated at 30°C for 24 h. This figure shows that E.coli JM109 (marA) cells that overexpress the marA product better than the control E.coli JM109 wild type cells survived plates overloyed by 30 % farnesol solution.

From the results, the device we built performs its desired function.