Difference between revisions of "Team:Nagahama/Proof"

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{{Nagahama}}
 
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<html>
 
<html>
  
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<style>
  
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<div class="column full_size judges-will-not-evaluate">
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/********************************* DEFAULT WIKI SETTINGS ********************************/
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<h3>★ ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">gold medal criterion for proof of concept</a>. </p>
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#sideMenu, #top_title {display:none;}
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#content { padding:0px; width:1000px; margin-top:-7px; margin-left:0px;}
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body {background-color:white; }
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#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
  
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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/********************************* MENU ********************************/
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<div class="column full_size">
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margin-top:-2px;
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<p>
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/* When hovering on a menu item */
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iGEM teams are great at making things work! We value teams not only doing an incredible job with theoretical models and experiments, but also in taking the first steps to make their project real.  
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.menu_items li:hover {
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</p>
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background-color:#72c9b6;
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cursor:pointer;
  
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<h4> What should we do for our proof of concept? </h4>
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}
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<p>
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You can assemble a device from BioBricks and show it works. You could build some equipment if you're competing for the hardware award. You can create a working model of your software for the software award. Please note that this not an exhaustive list of activities you can do to fulfill the gold medal criterion. As always, your aim is to impress the judges!
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</p>
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</div>
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/* moving the margin for the submenu ul list */
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ul.submenu {
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/*styling for the submenu buttons */
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margin-bottom: 0px;
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}
  
  
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</div>
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/* hover state for the submenu buttons */
 +
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padding: 5px 10px;
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border-bottom: 1px solid #d3d3d3;
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text-decoration:none;
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 +
 
 +
 
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.submenu li a:hover  {
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 +
background-color: #72c9b6;
 +
color:#000000; 
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}
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 +
/********************************* CONTENT OF THE PAGE ********************************/
 +
 
 +
/* Wrapper for the content */
 +
.content_wrapper {
 +
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 +
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 +
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 +
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 +
/*LAYOUT */
 +
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 +
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/*STYLING */
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.content_wrapper h1, .content_wrapper h2 {
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 +
.content_wrapper p {
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
 
 +
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 +
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 +
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 +
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 +
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 +
 
 +
/********************************* RESPONSIVE STYLING ********************************/
 +
 
 +
/* IF THE SCREEN IS LESS THAN 1000PX */
 +
 
 +
@media only screen and (max-width: 1000px) {
 +
 
 +
#content {width:100%; }
 +
.menu_wrapper {width:15%;}
 +
.content_wrapper {margin-left: 15%;}
 +
.menu_item {display:block;}
 +
.icon {display:none;}
 +
.highlight {padding:10px 0px;}
 +
}
 +
 
 +
 
 +
/* IF THE SCREEN IS LESS THAN 680PX */
 +
 
 +
@media only screen and (max-width: 680px) {
 +
.collapsable_menu_control { display:block;}
 +
.menu_item {display:none;}
 +
.menu_wrapper { width:100%; height: 15%; position:relative;}
 +
.content_wrapper {width:100%; margin-left:0px;}
 +
.column.half_size {width:100%; }
 +
.column img { width: 100%; padding: 5px 0px;}
 +
.icon {display:block;}
 +
.highlight {padding:15px 5px;}
 +
}
 +
 
 +
</style>
 +
 
 +
 
 +
 
 +
 
 +
<!--- THIS IS WHERE THE HTML BEGINS --->
 +
 
 +
 
 +
<!-- This tells the browser that your page is responsive -->
 +
 
 +
<head>
 +
<meta name="viewport" content="width=device-width, initial-scale=1">
 +
</head>
 +
 
 +
 
 +
 
 +
 
 +
<div class="menu_wrapper" >
 +
 
 +
 
 +
 
 +
 
 +
<div class="collapsable_menu_control"> MENU ▤ </div>
 +
 
 +
<ul id="accordion" class="accordion">
 +
 
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama">HOME </a> </li>
 +
 
 +
<li class="menu_item"> <div class="icon plus"></div> PROJECT 
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Introduction">  Introduction </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Result and Discussion"> Result and Discussion </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Future"> Future </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Protocol"> Protocol </a></li>
 +
                    </ul>
 +
                </li>
 +
 
 +
<li class="menu_item"> <div class="icon plus"></div> BioBricks 
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Our all BioBricks">  Our all BioBricks </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/BioBricks for medal"> BioBricks for medal </a></li>
 +
                    </ul>
 +
                </li>
 +
 
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama/Safety"> Safety </a> </li>
 +
 
 +
 
 +
<li class="menu_item"> <div class="icon plus"></div> Attributions
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Team">  Team</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Attributions"> Attributions </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Sponsors"> Sponsors</a></li>
 +
 
 +
                    </ul>
 +
                </li>
 +
 
 +
 
 +
<li class="menu_item"> <div class="icon plus"></div> Collaborations
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Collaborations">  Collaborations</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/The Annual Meeting Of The Genetics Society Of Japan"> The Annual Meeting Of The Genetics Society Of Japan </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Support Gifu"> Support Gifu</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/virginia">  Virginia</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Paris-Saclay">  Paris-Saclay</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Munich United"> Munich United</a></li>
 +
 
 +
 
 +
 
 +
 
 +
                    </ul>
 +
                </li>
 +
 
 +
<li class="menu_item"> <div class="icon plus"></div> Human Practice
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Human Practice"> Human Practice </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Local"> Contacting Local</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Japan"> Contacting Japan</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Intgrated Human Practice"> Intgrated Human Practice</a></li>
 +
 
 +
 
 +
 
 +
                    </ul>
 +
                </li>
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama/Proof of concept"> Proof of concept </a> </li>
 +
 
 +
 
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama/Demonstrate your work"> Demonstrate your work </a> </li>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 +
 
 +
 
 +
              　　　　　　　</ul>
 +
　　        </li>
 +
 
 +
 
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<div class="content_wrapper">
 +
 
 +
 
 +
 
 +
<h1 id="team_name">  </h1>
 +
<h4 id="page_name">  </h4>
 +
 
 +
 
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<script>
 +
 
 +
// This is the jquery part of your template.  Try not modify any of this code since it makes your menu work.
 +
 
 +
$(document).ready(function() {
 +
 
 +
$("#HQ_page").attr('id','');
 +
 +
 
 +
if ( wgPageName.substring( 0,  8) == "Template")  {  // if the page is a template it displays the full name in a single line
 +
$("#team_name").html( wgPageName );
 +
}
 +
 
 +
else if (  ( (wgPageName.match(/\//g) || []).length ) == 0 ) {  // if it is the home page , just print the team's name
 +
$("#team_name").html( wgPageName.substring( 5, wgPageName.length ) );
 +
}
 +
 
 +
else {
 +
// this adds the team's name as an h1
 +
$("#team_name").html( wgPageName.substring( 5 , wgPageName.indexOf("/")  ) );
 +
 
 +
// this adds the page's title as an h4
 +
$("#page_name").html (    ( wgPageName.substring( wgPageName.indexOf("/") + 1, wgPageName.length ) ).replace( /\/|_/g , " ")  );
 +
}
 +
 
 +
 
 +
 
 +
 
 +
$('#accordion').find('.menu_item').click(function(){
 +
 
 +
//Expand or collapse this panel
 +
submenu = $(this).find('.submenu');
 +
submenu.toggle();
 +
 
 +
icon = $(this).find('.icon');
 +
 
 +
if ( !$( submenu ).is(':visible') ) {
 +
icon.removeClass("less").addClass("plus");
 +
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 +
else {
 +
icon.removeClass("plus").addClass("less");
 +
}
 +
 
 +
//Hide the other panels
 +
$(".submenu").not(submenu).hide();
 +
$(".icon").not(icon).removeClass("less").addClass("plus");
 +
});
 +
 
 +
 
 +
$(".collapsable_menu_control").click(function() {
 +
$(".menu_item").toggle();
 +
});
 +
 
 +
$( window ).resize(function() {
 +
$(".menu_item").show();
 +
});
 +
 
 +
 
 +
});
 +
 
 +
 
 +
 
 +
 
 +
</script>
 +
 
  
 
</html>
 
</html>
 +
ＭarA<br>
 +
This device makes Escherichia coli have the resistance of farnesol.
 +
Actually, we confirmed resistance of farnesol by MarA.
 +
=='''Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates. '''==
 +
[[File:E.colimarA result .jpg|800px|thumb|center|Fig.7:Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.
 +
<br>
 +
 +
''E.coli'' JM109 and ''E.coli'' JM109 (marA) were dropped on LBGMg agar plates in serial ten-fold dilutions (10⁻¹～〖10〗^(-6)), overlaid with 30 % (v/v)　farnesol hexane solution (farnesol solution) and incubated at 30°C for 24 h. This figure shows that ''E.coli'' JM109 (marA) cells that overexpress the marA product better than the control ''E.coli'' JM109 wild type cells survived plates overloyed by 30 % farnesol solution.
 +
]]
 +
From the results, the device we built performs its desired function.

Latest revision as of 15:43, 19 October 2016

MENU ▤
　　

ＭarA
This device makes Escherichia coli have the resistance of farnesol. Actually, we confirmed resistance of farnesol by MarA.

Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.

Fig.7:Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.
E.coli JM109 and E.coli JM109 (marA) were dropped on LBGMg agar plates in serial ten-fold dilutions (10⁻¹～〖10〗^(-6)), overlaid with 30 % (v/v)　farnesol hexane solution (farnesol solution) and incubated at 30°C for 24 h. This figure shows that E.coli JM109 (marA) cells that overexpress the marA product better than the control E.coli JM109 wild type cells survived plates overloyed by 30 % farnesol solution.

From the results, the device we built performs its desired function.