Difference between revisions of "Team:Nagahama/Our all BioBricks"

 
(5 intermediate revisions by 4 users not shown)
Line 378: Line 378:
 
<li class="menu_item"> <div class="icon plus"></div> PROJECT   
 
<li class="menu_item"> <div class="icon plus"></div> PROJECT   
 
<ul class="submenu">
 
<ul class="submenu">
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Introduction">  Introduction </a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Introduction">  ・Introduction </a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Result and Discussion"> Result and Discussion </a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Result and Discussion"> ・Result and Discussion </a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Future"> Future </a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Future"> ・Future </a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Protocol"> Protocol </a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Protocol"> ・Protocol </a></li>
 
                     </ul>
 
                     </ul>
 
                 </li>
 
                 </li>
Line 387: Line 387:
 
<li class="menu_item"> <div class="icon plus"></div> BioBricks   
 
<li class="menu_item"> <div class="icon plus"></div> BioBricks   
 
<ul class="submenu">
 
<ul class="submenu">
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Our all BioBricks">  Our all BioBricks </a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Our all BioBricks">  ・Our all BioBricks </a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/BioBricks for medal"> BioBricks for medal </a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/BioBricks for medal"> ・BioBricks for medal </a></li>
 
                     </ul>
 
                     </ul>
 
                 </li>
 
                 </li>
Line 397: Line 397:
 
<li class="menu_item"> <div class="icon plus"></div> Attributions
 
<li class="menu_item"> <div class="icon plus"></div> Attributions
 
<ul class="submenu">
 
<ul class="submenu">
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Team">  Team</a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Team">  ・Team</a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Attributions"> Attributions </a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Attributions"> ・Attributions </a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Sponsors"> Sponsors</a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Sponsors"> ・Sponsors</a></li>
  
 
                     </ul>
 
                     </ul>
Line 407: Line 407:
 
<li class="menu_item"> <div class="icon plus"></div> Collaborations
 
<li class="menu_item"> <div class="icon plus"></div> Collaborations
 
<ul class="submenu">
 
<ul class="submenu">
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Collaborations">  Collaborations</a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Collaborations">  ・Collaborations</a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/The Annual Meeting Of The Genetics Society Of Japan"> The Annual Meeting Of The Genetics Society Of Japan </a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/The Annual Meeting Of The Genetics Society Of Japan"> ・The Annual Meeting Of The Genetics Society Of Japan </a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Support Gifu"> Support Gifu</a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Support Gifu"> ・Support Gifu</a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/virginia">  Virginia</a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/virginia">  ・Virginia</a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Paris-Saclay">  Paris-Saclay</a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Paris-Saclay">  ・Paris-Saclay</a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Munich United"> Munich United</a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Munich United"> ・Munich United</a></li>
  
  
Line 422: Line 422:
 
<li class="menu_item"> <div class="icon plus"></div> Human Practice
 
<li class="menu_item"> <div class="icon plus"></div> Human Practice
 
<ul class="submenu">
 
<ul class="submenu">
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Human Practice"> Human Practice </a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Human Practice"> ・Human Practice </a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Local"> Contacting Local</a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Local"> ・Contacting Local</a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Japan"> Contacting Japan</a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Japan"> ・Contacting Japan</a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Intgrated Human Practice"> Intgrated Human Practice</a></li>
+
<li> <a href="https://2016.igem.org/Team:Nagahama/Intgrated Human Practice"> ・Intgrated Human Practice</a></li>
−
<li> <a href="https://2016.igem.org/Team:Nagahama/Engagement"> Engagement</a></li>
+
−
 
+
  
  
Line 539: Line 537:
 
</html>
 
</html>
  
−
BioBrick Parts to achieve each medal requirement
 
−
 
−
Bronze
 
−
We created 3 new BioBricks [１ ](https://2016.igem.org/Team:Nagahama/Our_all_BioBricks) for bronze medal criterion. And have documented and submitted them to igem Registry.
 
−
 We introduce yajo (http://parts.igem.org/Part:BBa_K1950005) of them. This biobrick is Key BioBrics in “Flavorator” project. Yajo encodoes novel 1-deoxyxylulose-5-phosphate synthase. The novel 1-deoxyxylulose-5-phosphate synthase is able to synthesize 1-deoxy-D-xylulose 5-phosphate (DXP) from ribulose 5-phosphate, proving a novel route from a pentose phosphate to DXP 　Therefor the enzyme can catalyze  in the polyisoprenoid biosynthetic pathway.
 
−
 
−
Silver
 
−
We created 3 new BioBrick devices for silver medal criterion.
 
−
2.RT-PCR
 
−
2-1:PgpB.dev
 
−
2-2:YbjG.dev
 
−
To realize "Flovorator" we need to make E. coli produce Farnesol.
 
−
We have succeeded in confirming amount of transcription of PgpB.dev and YbjG.dev .
 
−
Our new biobrick device “PgpB.dev” and ”YbjG.dev” worked as expected.
 
−
Because we validate this fact by RT-PCR analysis experimentally. We have documented and submitted this new Biobrick device (http://parts.igem.org/Part:BBa_K1950007)　（http://parts.igem.org/Part:BBa_K1950008） to iGEM Registry .
 
−
 
−
デバイスの図を入れる
 
−
 
−
We want to make the E. coli produces farnesol which are one of the terpenes.
 
−
To produce great quantity of terpenes they need many terpene precursor and Farnesol synthase.
 
−
E. coli produces a small amount of the terpene precursor in MEP pathway.
 
−
In addition, E. coli has dephosphorylation enzyme of endogenous.
 
−
This is likely to synthesize farnesol from Farnesyl diphosphate (FPP), which is a precursor of farnesol.
 
−
 
−
In MEP pathway, there are four enzymes (ispD, ispF, idi, dxs) which are speed limiting enzyme for terpenes precursors produce in E. coli.
 
−
Last year, in order to, create a high-yield strains producing IPP and DMAPP, we exogenously engineered to superimpose these genes into E. coli to create strains overproducing IPP and DMAPP.
 
−
This year, in order to generate a large amount of further farnesol, exogenously engineer genes coding for farnesol enzyme synthesis , YbjG and PgpB, as farnesol synthesis in E. coli.
 
−
They are endogenous dephosphorylation enzyme of E. coli.
 
−
Farnesol is generated through FPP hydrolysis by PgpB and YbjG of endogenous phosphatases , which are induced by an increased intracellular FPP level.←○
 
−
To confirm whether the transcription of these genes is going well or not, we confirmed amount of the transcription of these genes by RT-PCR.
 
−
 
−
Result:（fig◯）
 
−
 
−
Discussion:
 
−
 
−
RT-PCRの結果の図
 
−
PgpB.dev
 
−
・YbjG.dev
 
−
 
−
Gold
 
−
marA :The activator of AcrAB-TolC multidrug efflux pump exports some terpenes
 
−
We improved the characterization of a previously existing BioBrick Part BBa_K1230000 and (http://parts.igem.org/Part:BBa_K1230000)　the characterization of a previously existing BioBrick marAgene as BBa_K1653006 . In existing part's information of marA, it gives E. coli resistance against kanamycin and give E-Coli resistance against geraniol as one of the terpene and decrease its intracellular concentration. In this year, we confirmed that overexpressing of marA gives E. coli resistance against farnesol as one of the terpene. This information is very beneficial for other iGEMers to production of toxic organic substances that are produced using bacteria.
 
−
We introduce an activator gene of AcrAB-TolC efflux pump (MarA) to release the farnesol from the cells and increase the content in the media that shows increase these flavors in the "Flavolator". In our study, we confirmed that overexpressing of marA gives host E. coli high resistance against farnesol.
 
−
図　まーえーのけっかはる
 
−
Farnesol production device（http://parts.igem.org/Part:BBa_K1653025）
 
−
 
−
To realize "Flovorator" we need to make E. coli produce Farnesol. Surprisingly, last year, we have succeeded in farnesol production by Farnesol production device. Our previous biobrick device "Farnesol production device" worked as expected. Because we validated this fact by GC-MS analysis experimentally.
 
−
We confirmed expression level of genes used in the 2015 fiscal year project for reference of farnesol production increase.
 
−
We improved the characterization of a previously existing BioBrick Part BBa_K1653025
 
−
In existing part's information of farnesol production device, it gives E. coli farnesol production. In this year,
 
  
−
This information is very beneficial for other iGEMers to farnesol that are produced using bacteria.
 
  
−
デバイスの図を入れる
+
<groupparts>iGEM016 Nagahama</groupparts>
−
結果の図を入れる
+

Latest revision as of 15:56, 19 October 2016

MENU ▤
　　


<groupparts>iGEM016 Nagahama</groupparts>