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<li class="menu_item"> <div class="icon plus"></div> PROJECT | <li class="menu_item"> <div class="icon plus"></div> PROJECT | ||
<ul class="submenu"> | <ul class="submenu"> | ||
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Introduction"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Introduction"> ・Introduction </a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Result and Discussion"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Result and Discussion"> ・Result and Discussion </a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Future"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Future"> ・Future </a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Protocol"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Protocol"> ・Protocol </a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<li class="menu_item"> <div class="icon plus"></div> BioBricks | <li class="menu_item"> <div class="icon plus"></div> BioBricks | ||
<ul class="submenu"> | <ul class="submenu"> | ||
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Our all BioBricks"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Our all BioBricks"> ・Our all BioBricks </a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/BioBricks for medal"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/BioBricks for medal"> ・BioBricks for medal </a></li> |
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<li class="menu_item"> <div class="icon plus"></div> Attributions | <li class="menu_item"> <div class="icon plus"></div> Attributions | ||
<ul class="submenu"> | <ul class="submenu"> | ||
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Team"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Team"> ・Team</a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Attributions"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Attributions"> ・Attributions </a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Sponsors"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Sponsors"> ・Sponsors</a></li> |
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<li class="menu_item"> <div class="icon plus"></div> Collaborations | <li class="menu_item"> <div class="icon plus"></div> Collaborations | ||
<ul class="submenu"> | <ul class="submenu"> | ||
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Collaborations"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Collaborations"> ・Collaborations</a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/The Annual Meeting Of The Genetics Society Of Japan"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/The Annual Meeting Of The Genetics Society Of Japan"> ・The Annual Meeting Of The Genetics Society Of Japan </a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Support Gifu"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Support Gifu"> ・Support Gifu</a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/virginia"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/virginia"> ・Virginia</a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Paris-Saclay"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Paris-Saclay"> ・Paris-Saclay</a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Munich United"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Munich United"> ・Munich United</a></li> |
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<li class="menu_item"> <div class="icon plus"></div> Human Practice | <li class="menu_item"> <div class="icon plus"></div> Human Practice | ||
<ul class="submenu"> | <ul class="submenu"> | ||
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Human Practice"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Human Practice"> ・Human Practice </a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Local"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Local"> ・Contacting Local</a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Japan"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Japan"> ・Contacting Japan</a></li> |
− | <li> <a href="https://2016.igem.org/Team:Nagahama/Intgrated Human Practice"> | + | <li> <a href="https://2016.igem.org/Team:Nagahama/Intgrated Human Practice"> ・Intgrated Human Practice</a></li> |
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+ | MarA<br> | ||
+ | This device makes Escherichia coli have the resistance of farnesol. | ||
+ | Actually, we confirmed resistance of farnesol by MarA. | ||
+ | =='''Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates. '''== | ||
+ | [[File:E.colimarA result .jpg|800px|thumb|center|Fig.7:Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates. | ||
+ | <br> | ||
+ | |||
+ | ''E.coli'' JM109 and ''E.coli'' JM109 (marA) were dropped on LBGMg agar plates in serial ten-fold dilutions (10⁻¹~〖10〗^(-6)), overlaid with 30 % (v/v) farnesol hexane solution (farnesol solution) and incubated at 30°C for 24 h. This figure shows that ''E.coli'' JM109 (marA) cells that overexpress the marA product better than the control ''E.coli'' JM109 wild type cells survived plates overloyed by 30 % farnesol solution. | ||
+ | ]] | ||
+ | From the results, the device we built performs its desired function. |
Latest revision as of 16:12, 19 October 2016