Difference between revisions of "Team:Nagahama/Proof of concept"

 
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<li class="menu_item"> <div class="icon plus"></div> PROJECT   
 
<li class="menu_item"> <div class="icon plus"></div> PROJECT   
 
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<ul class="submenu">
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<li> <a href="https://2016.igem.org/Team:Nagahama/Introduction">  Introduction </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Introduction">  ・Introduction </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Result and Discussion"> Result and Discussion </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Result and Discussion"> ・Result and Discussion </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Future"> Future </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Future"> ・Future </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Protocol"> Protocol </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Protocol"> ・Protocol </a></li>
 
                     </ul>
 
                     </ul>
 
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                 </li>
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<li class="menu_item"> <div class="icon plus"></div> BioBricks   
 
<li class="menu_item"> <div class="icon plus"></div> BioBricks   
 
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<li> <a href="https://2016.igem.org/Team:Nagahama/Our all BioBricks">  Our all BioBricks </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Our all BioBricks">  ・Our all BioBricks </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/BioBricks for medal"> BioBricks for medal </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/BioBricks for medal"> ・BioBricks for medal </a></li>
 
                     </ul>
 
                     </ul>
 
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<li class="menu_item"> <div class="icon plus"></div> Attributions
 
<li class="menu_item"> <div class="icon plus"></div> Attributions
 
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<li> <a href="https://2016.igem.org/Team:Nagahama/Team">  Team</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Team">  ・Team</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Attributions"> Attributions </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Attributions"> ・Attributions </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Sponsors"> Sponsors</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Sponsors"> ・Sponsors</a></li>
  
 
                     </ul>
 
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<li class="menu_item"> <div class="icon plus"></div> Collaborations
 
<li class="menu_item"> <div class="icon plus"></div> Collaborations
 
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<li> <a href="https://2016.igem.org/Team:Nagahama/Collaborations">  Collaborations</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Collaborations">  ・Collaborations</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/The Annual Meeting Of The Genetics Society Of Japan"> The Annual Meeting Of The Genetics Society Of Japan </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/The Annual Meeting Of The Genetics Society Of Japan"> ・The Annual Meeting Of The Genetics Society Of Japan </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Support Gifu"> Support Gifu</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Support Gifu"> ・Support Gifu</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/virginia">  Virginia</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/virginia">  ・Virginia</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Paris-Saclay">  Paris-Saclay</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Paris-Saclay">  ・Paris-Saclay</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Munich United"> Munich United</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Munich United"> ・Munich United</a></li>
  
  
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<li class="menu_item"> <div class="icon plus"></div> Human Practice
 
<li class="menu_item"> <div class="icon plus"></div> Human Practice
 
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<li> <a href="https://2016.igem.org/Team:Nagahama/Human Practice"> Human Practice </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Human Practice"> ・Human Practice </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Local"> Contacting Local</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Local"> ・Contacting Local</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Japan"> Contacting Japan</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Japan"> ・Contacting Japan</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Intgrated Human Practice"> Intgrated Human Practice</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Intgrated Human Practice"> ・Intgrated Human Practice</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Engagement"> Engagement</a></li>
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Actually, we confirmed resistance of farnesol by MarA.
 
Actually, we confirmed resistance of farnesol by MarA.
 
=='''Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates. '''==
 
=='''Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates. '''==
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[[File:E.colimarA result .jpg|800px|thumb|center|Fig.7:Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.
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[[File:E.colimarA result .jpg|800px|thumb|center|Fig.7:Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.
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<br>
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''E.coli'' JM109 and ''E.coli'' JM109 (marA) were dropped on LBGMg agar plates in serial ten-fold dilutions (10⁻¹～〖10〗^(-6)), overlaid with 30 % (v/v)　farnesol hexane solution (farnesol solution) and incubated at 30°C for 24 h. This figure shows that ''E.coli'' JM109 (marA) cells that overexpress the marA product better than the control ''E.coli'' JM109 wild type cells survived plates overloyed by 30 % farnesol solution.
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]]
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From the results, the device we built performs its desired function.

Latest revision as of 16:12, 19 October 2016

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ＭarA
This device makes Escherichia coli have the resistance of farnesol. Actually, we confirmed resistance of farnesol by MarA.

Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.

Fig.7:Colony formation of E. coli JM109 engineered with marA on farnesol overlaid plates.
E.coli JM109 and E.coli JM109 (marA) were dropped on LBGMg agar plates in serial ten-fold dilutions (10⁻¹～〖10〗^(-6)), overlaid with 30 % (v/v)　farnesol hexane solution (farnesol solution) and incubated at 30°C for 24 h. This figure shows that E.coli JM109 (marA) cells that overexpress the marA product better than the control E.coli JM109 wild type cells survived plates overloyed by 30 % farnesol solution.

From the results, the device we built performs its desired function.