Difference between revisions of "Team:KAIT Japan/Labnote"

Latest revision as of 19:08, 19 October 2016

Labonote

8/17

Restriction emzyme fragmation
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara
Transcription

8/18

Transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051

8/23

restriction emzyme fragmation

8/24

3A assembly
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051

8/25

Miniprep
J04450

8/26

Restriction enzyme treatment

8/29

Purity a gel

8/30

PCR

8/31

PCR
3A assembly
K595024 + I14044

9/1

PCR
K595024 I14044 C0051 R0010 B0015
purity a gel
preculture
K595024 I14044 C0051 R0010 B0015

9/2

Miniprep
K592024 I14044 C0051 R0010 B0015
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/3

Miniprep
K592024 I14044 C0051 R010 B0015 J04450

9/5

Restriction enzyme treatment

9/6

PCR
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/7

Miniprep
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/8

Miniprep
K592024 I14044 C0051 R010 B0015 J04450

9/8

PCR

9/12

Gel purification

9/13

Restriction enzyme treatment
C0051 R010 B0015X B0015E J04450
Pre-culture
K592024 I14044 J04450
Restriction enzyme treatment

9/14

Restriction enzyme treatment
Miniprep
K592024 I14044 J04450

9/15

Transformation
Restriction enzyme treatment
K592024 I14044 J04450

9/16

PCR
K592024 I14044 J04450
Miniprep
Restriction emzyme fragmation

9/19

Miniprep

9/21

Miniprep

9/22

Restriction emzyme fragmation
Miniprep
Purify a gel

9/23

Miniprep
Purify a gel

9/24

Restriction emzyme fragmation

9/25

Purify a gel
Ligation
Transformation

9/26

Miniprep

9/27

Purify a gel
Restriction emzyme fragmation

9/28

Restriction emzyme fragmation

9/30

Restriction emzyme fragmation
Purify a gel

10/1

Ligation
transformation
Pre-culture
Purification of antisensence

10/2

Pre-culture
Miniprep
Electrophoresis to confirm

10/3

miniprep
PCR
Pre-culture
Purify a gel

10/4

Restriction emzyme fragmation
Purify a gel
Miniprep
Electrophoresis to confirm

10/5

Ligation
Transformation
Purify a gel
Restriction emzyme fragmation

10/6

KIB…miniprep
Electrophoresis to confirm
Purify a gel

10/7

Restriction emzyme fragmation
Ligation
Transformation

10/8

Miniprep
Purify a gel
Restriction emzyme fragmation

10/9

Pre-culture
Purify a gel

10/10

Ligation
Transformation

10/11

pre-culture
Miniprep

10/12

Purify a gel

10/13

Electrophoresis to confirm

10/14

Restriction emzyme fragmation

10/15

Electrophoresis to confirm
Restriction emzyme fragmation

10/16

Electrophoresis to confirm

@@ Line 2: / Line 2: @@
 <html>
-/17<br>
-/16<br>
+<h2>Labonote</h2>
-miniprep<br>
-restriction emzyme fragmation<br>
+<br>
-/19<br>
-miniprep<br>
+<dt>8/17<br>
-/21<br>
+<dd>Restriction emzyme fragmation<br>
-miniprep<br>
+K592024 B0015 C0051→Eco RI & SpeI<br>
-/22<br>
+I14144 R100 B0015 →XbaI & PstI<br>
-restriction emzyme fragmation<br>
+EcoRI XbaI PstI →Promega<br>
-miniprep<br>
+SpeI→Takara<br>
-purify a gel<br>
+Transcription<br>
-/23<br>
-miniprep<br>
+<br>
-purify a gel<br>
+<dt>8/18<br>
-/24<br>
+<dd>Transcription<br>
-restriction emzyme fragmation<br>
+R0051 K592024 I14044 B0015 R0010<br>
-/25<br>
+C0051(psbic3)→E・coli-DH5X<br>
-purify a gel<br>
-ligation<br>
-transformation<br>
-/26<br>
-miniprep<br>
-/27<br>
-purify a gel<br>
-restriction emzyme fragmation<br>
-/28<br>
-restriction emzyme fragmation<br>
-/30<br>
-restriction emzyme fragmation<br>
-purify a gel<br>
-/1<br>
-ligation<br>
-transformation<br>
-pre-culture<br>
-purification of antisensence<br>
-/2<br>
-pre-culture<br>
-miniprep<br>
-electrophoresis to confirm<br>
-/3<br>
 miniprep<br>
+J04450<br>
+colony PCR<br>
+K592024 I14044 B0015 R0010 C0051 <br>
+<br>
+<dt>8/23<br>
+<dd>restriction emzyme fragmation<br>
+<br>
+<dt>8/24<br>
+<dd>3A assembly<br>
+Ligation<br>
 PCR<br>
-pre-culture<br>
+R0051 K592024 I14044 B0015 R0010 C0051 <br>
-purify a gel<br>
-/4<br>
+<br>
-restriction emzyme fragmation<br>
+<dt>8/25<br>
-purify a gel<br>
+<dd>Miniprep<br>
-miniprep<br>
+J04450<br>
-electrophoresis to confirm<br>
-/5<br>
+<br>
-ligation<br>
+<dt>8/26<br>
+<dd>Restriction enzyme treatment<br>
+<br>
+<dt>8/29<br>
+<dd>Purity a gel <br>
+<br>
+<dt>8/30<br>
+<dd>PCR<br>
+<br>
+<dt>8/31<br>
+<dd>PCR<br>
+A assembly<br>
+K595024 + I14044<br>
+<br>
+<dt>9/1<br>
+<dd>PCR<br>
+K595024 I14044 C0051 R0010 B0015 <br>
+purity a gel <br>
+preculture<br>
+K595024 I14044 C0051 R0010 B0015 <br>
+<br>
+<dt>9/2<br>
+<dd>Miniprep<br>
+K592024 I14044 C0051 R0010 B0015<br>
+Pre-culture<br>
+K592024 I14044 C0051 R010 B0015 J04450<br>
+<br>
+<dt>9/3<br>
+<dd>Miniprep<br>
+K592024 I14044 C0051 R010 B0015 J04450<br>
+<br>
+<dt>9/5<br>
+<dd>Restriction enzyme treatment<br>
+<br>
+<dt>9/6<br>
+<dd>PCR<br>
+Restriction enzyme treatment<br>
+Pre-culture<br>
+K592024 I14044 C0051 R010 B0015 J04450<br>
+<br>
+<dt>9/7
+<dd>Miniprep<br>
+K592024 I14044 C0051 R010 B0015 J04450<br>
+Restriction enzyme treatment<br>
+Pre-culture<br>
+K592024 I14044 C0051 R010 B0015 J04450<br>
+<br>
+<dt>9/8<br>
+<dd>Miniprep<br>
+K592024 I14044 C0051 R010 B0015 J04450<br>
+<br>
+<dt>9/8<br>
+<dd>PCR<br>
+<br>
+<dt>9/12<br>
+<dd>Gel purification<br>
+<br>
+<dt>9/13<br>
+<dd>Restriction enzyme treatment<br>
+C0051 R010 B0015X B0015E J04450<br>
+Pre-culture<br>
+K592024 I14044 J04450<br>
+Restriction enzyme treatment<br>
+<br>
+<dt>9/14<br>
+<dd>Restriction enzyme treatment<br>
+Miniprep<br>
+K592024 I14044 J04450<br>
+<br>
+<dt>9/15<br>
+<dd>Transformation<br>
+Restriction enzyme treatment<br>
+K592024 I14044 J04450<br>
+<br>
+<dt>9/16<br>
+<dd>PCR<br>
+K592024 I14044 J04450<br>
+Miniprep<br>
+Restriction emzyme fragmation<br>
+<br>
+<dt>9/19<br>
+<dd>Miniprep<br>
+<br>
+<dt>9/21<br>
+<dd>Miniprep<br>
+<br>
+<dt>9/22<br>
+<dd>Restriction emzyme fragmation<br>
+Miniprep<br>
+Purify a gel<br>
+<br>
+<dt>9/23<br>
+<dd>Miniprep<br>
+Purify a gel<br>
+<br>
+<dt>9/24<br>
+<dd>Restriction emzyme fragmation<br>
+<br>
+<dt>9/25<br>
+<dd>Purify a gel<br>
+Ligation<br>
+Transformation<br>
+<br>
+<dt>9/26<br>
+<dd>Miniprep<br>
+<br>
+<dt>9/27<br>
+<dd>Purify a gel<br>
+Restriction emzyme fragmation<br>
+<br>
+<dt>9/28<br>
+<dd>Restriction emzyme fragmation<br>
+<br>
+<dt>9/30<br>
+<dd>Restriction emzyme fragmation<br>
+Purify a gel<br>
+<br>
+<dt>10/1<br>
+<dd>Ligation<br>
 transformation<br>
-purify a gel<br>
+Pre-culture<br>
-restriction emzyme fragmation<br>
+Purification of antisensence<br>
-/6<br>
-KIB…miniprep<br>
+<br>
-electrophoresis to confirm<br>
+<dt>10/2<br>
-purify a gel<br>
+<dd>Pre-culture<br>
-/7<br>
+Miniprep<br>
-restriction emzyme fragmation<br>
+Electrophoresis to confirm<br>
-ligation<br>
-transformation<br>
+<br>
-/8<br>
+<dt>10/3<br>
-miniprep<br>
+<dd>miniprep<br>
-purify a gel<br>
+PCR<br>
-restriction emzyme fragmation<br>
+Pre-culture<br>
-/9<br>
+Purify a gel<br>
-pre-culture<br>
-purify a gel<br>
+<br>
-/10<br>
+<dt>10/4<br>
-ligation<br>
+<dd>Restriction emzyme fragmation<br>
-transformation<br>
+Purify a gel<br>
-/11<br>
+Miniprep<br>
-pre-culture<br>
+Electrophoresis to confirm<br>
-miniprep<br>
-/12<br>
+<br>
-purify a gel<br>
+<dt>10/5<br>
-/13<br>
+<dd>Ligation<br>
-electrophoresis to confirm<br>
+Transformation<br>
-/14<br>
+Purify a gel<br>
-restriction emzyme fragmation<br>
+Restriction emzyme fragmation<br>
-/15<br>
-electrophoresis to confirm<br>
+<br>
-restriction emzyme fragmation<br>
+<dt>10/6<br>
-/16<br>
+<dd>KIB…miniprep<br>
-electrophoresis to confirm<br>
+Electrophoresis to confirm<br>
+Purify a gel<br>
+<br>
+<dt>10/7<br>
+<dd>Restriction emzyme fragmation<br>
+Ligation<br>
+Transformation<br>
+<br>
+<dt>10/8<br>
+<dd>Miniprep<br>
+Purify a gel<br>
+Restriction emzyme fragmation<br>
+<br>
+<dt>10/9<br>
+<dd>Pre-culture<br>
+Purify a gel<br>
+<br>
+<dt>10/10<br>
+<dd>Ligation<br>
+Transformation<br>
+<br>
+<dt>10/11<br>
+<dd>pre-culture<br>
+Miniprep<br>
+<br>
+<dt>10/12<br>
+<dd>Purify a gel<br>
+<br>
+<dt>10/13<br>
+<dd>Electrophoresis to confirm<br>
+<br>
+<dt>10/14<br>
+<dd>Restriction emzyme fragmation<br>
+<br>
+<dt>10/15<br>
+<dd>Electrophoresis to confirm<br>
+Restriction emzyme fragmation<br>
+<br>
+<dt>10/16<br>
+<dd>Electrophoresis to confirm<br>
+<br>
+</dl>
 <!--全てのページの下の部分にここから下をコピペしてください-->