(18 intermediate revisions by the same user not shown) | |||
Line 3: | Line 3: | ||
− | < | + | <h2>Labonote</h2> |
<br> | <br> | ||
− | 8/17<br> | + | |
− | + | <dt>8/17<br> | |
+ | <dd>Restriction emzyme fragmation<br> | ||
K592024 B0015 C0051→Eco RI & SpeI<br> | K592024 B0015 C0051→Eco RI & SpeI<br> | ||
I14144 R100 B0015 →XbaI & PstI<br> | I14144 R100 B0015 →XbaI & PstI<br> | ||
EcoRI XbaI PstI →Promega<br> | EcoRI XbaI PstI →Promega<br> | ||
SpeI→Takara<br> | SpeI→Takara<br> | ||
+ | Transcription<br> | ||
<br> | <br> | ||
− | 8/18<br> | + | <dt>8/18<br> |
− | + | <dd>Transcription<br> | |
R0051 K592024 I14044 B0015 R0010<br> | R0051 K592024 I14044 B0015 R0010<br> | ||
C0051(psbic3)→E・coli-DH5X<br> | C0051(psbic3)→E・coli-DH5X<br> | ||
− | |||
− | |||
− | |||
miniprep<br> | miniprep<br> | ||
J04450<br> | J04450<br> | ||
Line 27: | Line 26: | ||
<br> | <br> | ||
− | 8/23<br> | + | <dt>8/23<br> |
− | restriction emzyme fragmation<br> | + | <dd>restriction emzyme fragmation<br> |
<br> | <br> | ||
− | 8/24<br> | + | <dt>8/24<br> |
− | 3A assembly<br> | + | <dd>3A assembly<br> |
Ligation<br> | Ligation<br> | ||
PCR<br> | PCR<br> | ||
Line 38: | Line 37: | ||
<br> | <br> | ||
− | 8/25<br> | + | <dt>8/25<br> |
− | Miniprep<br> | + | <dd>Miniprep<br> |
J04450<br> | J04450<br> | ||
<br> | <br> | ||
− | 8/26<br> | + | <dt>8/26<br> |
− | Restriction enzyme treatment<br> | + | <dd>Restriction enzyme treatment<br> |
<br> | <br> | ||
− | 8/29<br> | + | <dt>8/29<br> |
− | Purity a gel <br> | + | <dd>Purity a gel <br> |
<br> | <br> | ||
− | 8/30<br> | + | <dt>8/30<br> |
− | PCR | + | <dd>PCR<br> |
<br> | <br> | ||
− | 8/31<br> | + | <dt>8/31<br> |
− | PCR<br> | + | <dd>PCR<br> |
3A assembly<br> | 3A assembly<br> | ||
K595024 + I14044<br> | K595024 + I14044<br> | ||
<br> | <br> | ||
− | 9/1<br> | + | <dt>9/1<br> |
− | PCR<br> | + | <dd>PCR<br> |
K595024 I14044 C0051 R0010 B0015 <br> | K595024 I14044 C0051 R0010 B0015 <br> | ||
purity a gel <br> | purity a gel <br> | ||
Line 69: | Line 68: | ||
<br> | <br> | ||
− | 9/2<br> | + | <dt>9/2<br> |
− | Miniprep<br> | + | <dd>Miniprep<br> |
K592024 I14044 C0051 R0010 B0015<br> | K592024 I14044 C0051 R0010 B0015<br> | ||
Pre-culture<br> | Pre-culture<br> | ||
Line 76: | Line 75: | ||
<br> | <br> | ||
− | 9/3<br> | + | <dt>9/3<br> |
− | Miniprep<br> | + | <dd>Miniprep<br> |
K592024 I14044 C0051 R010 B0015 J04450<br> | K592024 I14044 C0051 R010 B0015 J04450<br> | ||
<br> | <br> | ||
− | 9/5<br> | + | <dt>9/5<br> |
− | Restriction enzyme treatment<br> | + | <dd>Restriction enzyme treatment<br> |
<br> | <br> | ||
− | 9/6<br> | + | <dt>9/6<br> |
− | PCR<br> | + | <dd>PCR<br> |
Restriction enzyme treatment<br> | Restriction enzyme treatment<br> | ||
Pre-culture<br> | Pre-culture<br> | ||
Line 92: | Line 91: | ||
<br> | <br> | ||
− | 9/7 | + | <dt>9/7 |
− | Miniprep<br> | + | <dd>Miniprep<br> |
K592024 I14044 C0051 R010 B0015 J04450<br> | K592024 I14044 C0051 R010 B0015 J04450<br> | ||
Restriction enzyme treatment<br> | Restriction enzyme treatment<br> | ||
Line 100: | Line 99: | ||
<br> | <br> | ||
− | 9/8<br> | + | <dt>9/8<br> |
− | Miniprep<br> | + | <dd>Miniprep<br> |
K592024 I14044 C0051 R010 B0015 J04450<br> | K592024 I14044 C0051 R010 B0015 J04450<br> | ||
<br> | <br> | ||
− | 9/8<br> | + | <dt>9/8<br> |
− | PCR<br> | + | <dd>PCR<br> |
<br> | <br> | ||
− | 9/12<br> | + | <dt>9/12<br> |
− | Gel purification<br> | + | <dd>Gel purification<br> |
− | + | ||
− | + | ||
<br> | <br> | ||
− | 9/13<br> | + | <dt>9/13<br> |
− | Restriction enzyme treatment<br> | + | <dd>Restriction enzyme treatment<br> |
C0051 R010 B0015X B0015E J04450<br> | C0051 R010 B0015X B0015E J04450<br> | ||
Pre-culture<br> | Pre-culture<br> | ||
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Restriction enzyme treatment<br> | Restriction enzyme treatment<br> | ||
− | 9/14<br> | + | <br> |
− | Restriction enzyme treatment<br> | + | <dt>9/14<br> |
+ | <dd>Restriction enzyme treatment<br> | ||
Miniprep<br> | Miniprep<br> | ||
K592024 I14044 J04450<br> | K592024 I14044 J04450<br> | ||
<br> | <br> | ||
− | 9/15<br> | + | <dt>9/15<br> |
− | Transformation<br> | + | <dd>Transformation<br> |
Restriction enzyme treatment<br> | Restriction enzyme treatment<br> | ||
K592024 I14044 J04450<br> | K592024 I14044 J04450<br> | ||
<br> | <br> | ||
− | 9/16<br> | + | <dt>9/16<br> |
− | PCR<br> | + | <dd>PCR<br> |
K592024 I14044 J04450<br> | K592024 I14044 J04450<br> | ||
Miniprep<br> | Miniprep<br> | ||
Line 142: | Line 140: | ||
<br> | <br> | ||
− | 9/19<br> | + | <dt>9/19<br> |
− | Miniprep<br> | + | <dd>Miniprep<br> |
<br> | <br> | ||
− | 9/21<br> | + | <dt>9/21<br> |
− | Miniprep<br> | + | <dd>Miniprep<br> |
<br> | <br> | ||
− | 9/22<br> | + | <dt>9/22<br> |
− | Restriction emzyme fragmation<br> | + | <dd>Restriction emzyme fragmation<br> |
Miniprep<br> | Miniprep<br> | ||
Purify a gel<br> | Purify a gel<br> | ||
<br> | <br> | ||
− | 9/23<br> | + | <dt>9/23<br> |
− | Miniprep<br> | + | <dd>Miniprep<br> |
Purify a gel<br> | Purify a gel<br> | ||
<br> | <br> | ||
− | 9/24<br> | + | <dt>9/24<br> |
− | + | <dd>Restriction emzyme fragmation<br> | |
<br> | <br> | ||
− | 9/25<br> | + | <dt>9/25<br> |
− | + | <dd>Purify a gel<br> | |
− | + | Ligation<br> | |
− | + | Transformation<br> | |
<br> | <br> | ||
− | 9/26<br> | + | <dt>9/26<br> |
− | Miniprep<br> | + | <dd>Miniprep<br> |
<br> | <br> | ||
− | 9/27<br> | + | <dt>9/27<br> |
− | Purify a gel<br> | + | <dd>Purify a gel<br> |
Restriction emzyme fragmation<br> | Restriction emzyme fragmation<br> | ||
<br> | <br> | ||
− | 9/28<br> | + | <dt>9/28<br> |
− | Restriction emzyme fragmation<br> | + | <dd>Restriction emzyme fragmation<br> |
<br> | <br> | ||
− | 9/30<br> | + | <dt>9/30<br> |
− | Restriction emzyme fragmation<br> | + | <dd>Restriction emzyme fragmation<br> |
Purify a gel<br> | Purify a gel<br> | ||
<br> | <br> | ||
− | 10/1<br> | + | <dt>10/1<br> |
− | Ligation<br> | + | <dd>Ligation<br> |
transformation<br> | transformation<br> | ||
Pre-culture<br> | Pre-culture<br> | ||
Line 197: | Line 195: | ||
<br> | <br> | ||
− | 10/2<br> | + | <dt>10/2<br> |
− | Pre-culture<br> | + | <dd>Pre-culture<br> |
Miniprep<br> | Miniprep<br> | ||
Electrophoresis to confirm<br> | Electrophoresis to confirm<br> | ||
<br> | <br> | ||
− | 10/3<br> | + | <dt>10/3<br> |
− | miniprep<br> | + | <dd>miniprep<br> |
PCR<br> | PCR<br> | ||
Pre-culture<br> | Pre-culture<br> | ||
Line 210: | Line 208: | ||
<br> | <br> | ||
− | 10/4<br> | + | <dt>10/4<br> |
− | Restriction emzyme fragmation<br> | + | <dd>Restriction emzyme fragmation<br> |
Purify a gel<br> | Purify a gel<br> | ||
Miniprep<br> | Miniprep<br> | ||
Line 217: | Line 215: | ||
<br> | <br> | ||
− | 10/5<br> | + | <dt>10/5<br> |
− | Ligation<br> | + | <dd>Ligation<br> |
Transformation<br> | Transformation<br> | ||
Purify a gel<br> | Purify a gel<br> | ||
Line 224: | Line 222: | ||
<br> | <br> | ||
− | 10/6<br> | + | <dt>10/6<br> |
− | KIB…miniprep<br> | + | <dd>KIB…miniprep<br> |
Electrophoresis to confirm<br> | Electrophoresis to confirm<br> | ||
Purify a gel<br> | Purify a gel<br> | ||
<br> | <br> | ||
− | 10/7<br> | + | <dt>10/7<br> |
− | Restriction emzyme fragmation<br> | + | <dd>Restriction emzyme fragmation<br> |
Ligation<br> | Ligation<br> | ||
Transformation<br> | Transformation<br> | ||
<br> | <br> | ||
− | 10/8<br> | + | <dt>10/8<br> |
− | Miniprep<br> | + | <dd>Miniprep<br> |
Purify a gel<br> | Purify a gel<br> | ||
Restriction emzyme fragmation<br> | Restriction emzyme fragmation<br> | ||
<br> | <br> | ||
− | 10/9<br> | + | <dt>10/9<br> |
− | Pre-culture<br> | + | <dd>Pre-culture<br> |
Purify a gel<br> | Purify a gel<br> | ||
<br> | <br> | ||
− | 10/10<br> | + | <dt>10/10<br> |
− | + | <dd>Ligation<br> | |
Transformation<br> | Transformation<br> | ||
<br> | <br> | ||
− | 10/11<br> | + | <dt>10/11<br> |
− | pre-culture<br> | + | <dd>pre-culture<br> |
Miniprep<br> | Miniprep<br> | ||
<br> | <br> | ||
− | 10/12<br> | + | <dt>10/12<br> |
− | Purify a gel<br> | + | <dd>Purify a gel<br> |
<br> | <br> | ||
− | 10/13<br> | + | <dt>10/13<br> |
− | Electrophoresis to confirm<br> | + | <dd>Electrophoresis to confirm<br> |
<br> | <br> | ||
− | 10/14<br> | + | <dt>10/14<br> |
− | Restriction emzyme fragmation<br> | + | <dd>Restriction emzyme fragmation<br> |
<br> | <br> | ||
− | 10/15<br> | + | <dt>10/15<br> |
− | + | <dd>Electrophoresis to confirm<br> | |
− | + | Restriction emzyme fragmation<br> | |
<br> | <br> | ||
− | 10/16<br> | + | <dt>10/16<br> |
− | + | <dd>Electrophoresis to confirm<br> | |
<br> | <br> | ||
+ | </dl> | ||
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Latest revision as of 19:08, 19 October 2016
Labonote
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara
Transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051
J04450
3A assembly
K595024 + I14044
K595024 I14044 C0051 R0010 B0015
purity a gel
preculture
K595024 I14044 C0051 R0010 B0015
K592024 I14044 C0051 R0010 B0015
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
K592024 I14044 C0051 R010 B0015 J04450
C0051 R010 B0015X B0015E J04450
Pre-culture
K592024 I14044 J04450
Restriction enzyme treatment
Miniprep
K592024 I14044 J04450
Restriction enzyme treatment
K592024 I14044 J04450
K592024 I14044 J04450
Miniprep
Restriction emzyme fragmation
Miniprep
Purify a gel
Purify a gel
Ligation
Transformation
Restriction emzyme fragmation
Purify a gel
transformation
Pre-culture
Purification of antisensence
Miniprep
Electrophoresis to confirm
PCR
Pre-culture
Purify a gel
Purify a gel
Miniprep
Electrophoresis to confirm
Transformation
Purify a gel
Restriction emzyme fragmation
Electrophoresis to confirm
Purify a gel
Ligation
Transformation
Purify a gel
Restriction emzyme fragmation
Purify a gel
Transformation
Miniprep
Restriction emzyme fragmation