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Notes for this project were split into two categories of work. Please click the links below for full versions of the notes!
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<ul>
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&nbsp;&nbsp;&nbsp;&nbsp;<li><a href="https://static.igem.org/mediawiki/2016/7/7f/T--Duke--2016FinalCloningNotes.pdf">Cloning of Parts</a></li>
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&nbsp;&nbsp;&nbsp;&nbsp;<li><a href="https://static.igem.org/mediawiki/2016/7/78/T--Duke--2016FinalEnzymeAssayNotes.pdf">Characterization of Enzymes</a></li>
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</ul>
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<!--
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<html lang="en">
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<head>
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<meta charset="utf-8"/>
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<title>Lab Maintenance · Benchling</title>
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<link href="https://2016.igem.org/Template:Duke/Notebook/CSS?action=raw&ctype=text/css" rel="stylesheet"/>
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<body><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Lab Maintenance</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> Duke iGEM 2016</div><div><b>Authors:</b> Nisakorn Valyasevi</div><div><b>Dates:</b> 2016-05-18 to 2016-08-27</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 5/18/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part50" data-index="0" style="margin-left:0;">Made 2 L of LB Broth</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part51-item" data-formattable="true" data-index="1" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-QZmqpzMV-lb-brothmedia-protocol/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">LB Broth/Media Protocol</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part51" data-index="1">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part52" data-index="2" style="margin-left:0;">Autoclaved for 30 min. -- One liter was made between two sealable 1 L bottles (each filled with 500 mL of media)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part53" data-index="3" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part54" data-index="4" style="margin-left:0;">Made 1 L of LB Agar and LB Agar + KAN Plates</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part55-item" data-formattable="true" data-index="5" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-WVuO8pom-lb-agar-plates/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">LB Agar Plates</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part55" data-index="5">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part56" data-index="6" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Autoclaved for 30 min.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part57" data-index="7" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part58" data-index="8" style="margin-left:0;">Made 1 L of 10% Glycerol and 500 mL of 50% Glycerol</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part59-item" data-formattable="true" data-index="9" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-hFEVxMi5-glycerol-stocks/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Glycerol Stocks</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part59" data-index="9">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part60" data-index="10" style="margin-left:0;">Autoclaved for 30 min.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part61" data-index="11" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part62" data-index="12" style="margin-left:0;">Made Kanamycin Stock</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part63-item" data-formattable="true" data-index="13" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-MrVh9OCN-antibiotic-stocks/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Antibiotic Stocks</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part63" data-index="13">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part64" data-index="14" style="margin-left:0;">Used 350 mg of Kanamycin to make a 1000X stock of 35 ug/mL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part65" data-index="15" style="margin-left:0;">Aliquoted into 1 mL epi tubes.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part66" data-index="16" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part67" data-index="17" style="margin-left:0;">Notes:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part68" data-index="18" style="margin-left:35px;">Shaker ("Max-Q 4000") set to 38; thermometer reads 33.2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part69" data-index="19" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part70" data-index="20" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 5/20/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part71" data-index="21" style="margin-left:0;">Made Ampicillin and Chloramphenicol Stocks (Nisa; Thomas)</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part72-item" data-formattable="true" data-index="22" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-MrVh9OCN-antibiotic-stocks/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Antibiotic Stocks</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part72" data-index="22">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part73" data-index="23" style="margin-left:0;">Made 7 mL of 200 mg/mL of Ampicillin</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part74" data-index="24" style="margin-left:0;">Made ~7 mL of 25 mg/mL of Chloramphenicol </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part75" data-index="25" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part76" data-index="26" style="margin-left:0;">Made KAN and CM Plates (Ben; Emma)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part77" data-index="27" style="margin-left:0;">50 KAN plates </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part78" data-index="28" style="margin-left:0;">35 CM plates</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part79-item" data-formattable="true" data-index="29" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-WVuO8pom-lb-agar-plates/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">LB Agar Plates</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part79" data-index="29">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part80" data-index="30" style="margin-left:0;">Made Gels</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part81-item" data-formattable="true" data-index="31" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-rp3pOq0z-gels/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Gels</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part81" data-index="31">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part82" data-index="32" style="margin-left:0;">Made 3 gels </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part83" data-index="33" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 5/24/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part84" data-index="34" style="margin-left:0;">Preparation of E. cloni Comp Cells (Parth; Nisa)</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part85-item" data-formattable="true" data-index="35" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-2cmHUaIk-electrocompetent-cells/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Electrocompetent Cells</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part85" data-index="35">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part86" data-index="36" style="margin-left:0;">Left culture overnight; did not check OD</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part87" data-index="37" style="margin-left:0;">Washed with glycerol 6 times</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part88" data-index="38" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part89" data-index="39" style="margin-left:0;"><span style="color:#000000">Forgot to do the final spin down and resuspension of equal volume of glycerol (these steps were not written in the </span> <span style="color:#000000">protocol at the time, protocol has been updated)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part90" data-index="40" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part91" data-index="41" style="margin-left:0;"><span style="color:#000000">Aliquoted 50 uL into 1.7 mL epi tubes</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part92" data-index="42" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part93" data-index="43" style="margin-left:0;">Made Gels</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part94" data-index="44" style="margin-left:0;">Made 2 more gels; wrapped in cling wrap and stored in 4 C fridge</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part95-item" data-formattable="true" data-index="45" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-rp3pOq0z-gels/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Gels</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part95" data-index="45">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part96" data-index="46" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 5/25/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part97" data-index="47" style="margin-left:0;">Made Gels</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part98-item" data-formattable="true" data-index="48" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-rp3pOq0z-gels/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Gels</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part98" data-index="48">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part99" data-index="49" style="margin-left:0;">Made 3 gels </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part100" data-index="50" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part101" data-index="51" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/1/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part102" data-index="52" style="margin-left:0;">Made KAN Plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part103" data-index="53" style="margin-left:0;">Stored most of KAN plates in bag in 4 C fridge -- the rest stored at room temp on shelf</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part104" data-index="54" style="margin-left:0;">Made 1 L of Agar for plates - all plates were KAN plate</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part105-item" data-formattable="true" data-index="55" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-WVuO8pom-lb-agar-plates/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">LB Agar Plates</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part105" data-index="55">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part106" data-index="56" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/2/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part107" data-index="57" style="margin-left:0;">Made Gels </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part108" data-index="58" style="margin-left:0;">2 Gels were made </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part109-item" data-formattable="true" data-index="59" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-rp3pOq0z-gels/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Gels</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part109" data-index="59">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part110" data-index="60" style="margin-left:0;">Autoclaved Tips and Glassware</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part111" data-index="61" style="margin-left:0;">Autoclaved 10 boxes of 200 tips and 5 boxes of 10 tips on gravity cycle</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part112" data-index="62" style="margin-left:0;">Autoclaved beakers and flasks</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part113" data-index="63" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part114" data-index="64" style="margin-left:0;">Made 20% Glycerol Stocks</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part115-item" data-formattable="true" data-index="65" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-hFEVxMi5-glycerol-stocks/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Glycerol Stocks</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part115" data-index="65">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part116" data-index="66" style="margin-left:0;">40 mL of 100% Glycerol was added to 160 mL pH2O</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part117" data-index="67" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part118" data-index="68" style="margin-left:0;">Freezer Purge</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part119" data-index="69" style="margin-left:0;">The freezer stocks were gone through and all negative sequence stocks were discarded.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part120" data-index="70" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/3/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part121" data-index="71" style="margin-left:0;">Made Gels</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part122" data-index="72" style="margin-left:0;">Made 2 new gels and left in fridge for weekend</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part123-item" data-formattable="true" data-index="73" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-rp3pOq0z-gels/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Gels</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part123" data-index="73">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part124" data-index="74" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 6/5/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part125" data-index="75" style="margin-left:0;">Gels for PCR (Jaydeep)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part126" data-index="76" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part127" data-index="77" style="margin-left:0;">Made 4 gels, 200mL total</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part128-item" data-formattable="true" data-index="78" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-rp3pOq0z-gels/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Gels</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part128" data-index="78">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part129" data-index="79" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/6/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part130" data-index="80" style="margin-left:0;">Gels for PCR (Ben; Jaydeep)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part131" data-index="81" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part132" data-index="82" style="margin-left:0;">Made 4 gels, 200mL tot</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part133-item" data-formattable="true" data-index="83" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-rp3pOq0z-gels/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Gels</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part133" data-index="83">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part134" data-index="84" style="margin-left:0;">
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</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/7/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part135" data-index="85" style="margin-left:0;">Made KAN Plates (Nisa; Parth; Thomas)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part136" data-index="86" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part137" data-index="87" style="margin-left:0;">Made 52 KAN plates</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part138-item" data-formattable="true" data-index="88" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-WVuO8pom-lb-agar-plates/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">LB Agar Plates</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part138" data-index="88">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part139" data-index="89" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/13/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part140" data-index="90" style="margin-left:0;">Made LB +KAN</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part141-item" data-formattable="true" data-index="91" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-QZmqpzMV-lb-brothmedia-protocol/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">LB Broth/Media Protocol</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part141" data-index="91">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part142" data-index="92" style="margin-left:0;">Made 4 bottles (250mL) of 125 mL aliquots of LB+KAN broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part143" data-index="93" style="margin-left:35px;">Used 1/8 of the LB media recipe in each bottle (0.625 g of Salt and Yeast and 1.25 g of tryptone)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part144" data-index="94" style="margin-left:0;">The old stocks had been contaminated</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part145" data-index="95" style="margin-left:0;">Used smaller bottles and smaller aliquots to limit contamination</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part146" data-index="96" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part147" data-index="97" style="margin-left:0;">Made Aliquots of Commonly Used Materials</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part148" data-index="98" style="margin-left:0;">Made conical tube aliquots (~50 mL) of 10%, 20%, and 50% Glycerol as well as pico water.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part149" data-index="99" style="margin-left:0;">Made aliquots to help limit contamination and to clean up bench space. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part150" data-index="100" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/14/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part151" data-index="101" style="margin-left:0;">Made CM Plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part152" data-index="102" style="margin-left:0;">Made 0.5 L of CM Plates - 23 plates </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part153-item" data-formattable="true" data-index="103" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-WVuO8pom-lb-agar-plates/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">LB Agar Plates</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part153" data-index="103">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part154" data-index="104" style="margin-left:0;">Made CM LB Stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part155" data-index="105" style="margin-left:0;">Made 0.5 L of LB Broth + CM</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part156-item" data-formattable="true" data-index="106" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-QZmqpzMV-lb-brothmedia-protocol/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">LB Broth/Media Protocol</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part156" data-index="106">
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part157" data-index="107" style="margin-left:0;">Autoclaved Glassware</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part158" data-index="108" style="margin-left:0;">New half liter and liter bottles came in. All were autoclaved. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part159" data-index="109" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part160" data-index="110" style="margin-left:0;">Made KAN Stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part161" data-index="111" style="margin-left:0;">Made KAN Stocks using 40 ng/ul. </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part162-item" data-formattable="true" data-index="112" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-MrVh9OCN-antibiotic-stocks/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Antibiotic Stocks</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part162" data-index="112">
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part163" data-index="113" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/15/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part164" data-index="114" style="margin-left:0;">Made E-cloni Stocks</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part165" data-index="115" style="margin-left:0;">Took old plate and inoculated two 50 mL LB flasks.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part166" data-index="116" style="margin-left:35px;">The plate had several infections </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part167" data-index="117" style="margin-left:0;">Took a long time to grow - suspicious </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part168" data-index="118" style="margin-left:0;">Decided to discard</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part169" data-index="119" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part170" data-index="120" style="margin-left:0;">Made new E-cloni Plate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part171" data-index="121" style="margin-left:0;">Because the old plate had several infections, a chip of stock Ecloni was plated onto a new plate and grown overnight</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part172" data-index="122" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/16/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part173" data-index="123" style="margin-left:0;">Made E-cloni Stocks</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part174" data-index="124" style="margin-left:0;">Inoculated two flasks of 50 mL LB </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part175" data-index="125" style="margin-left:0;">Let grow </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part176" data-index="126" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/17/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part177" data-index="127" style="margin-left:0;">Comp Cells were made </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part178-item" data-formattable="true" data-index="128" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-2cmHUaIk-electrocompetent-cells/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Electrocompetent Cells</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part178" data-index="128">Not yet tested
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part179" data-index="129" style="margin-left:0;">End of Week Check</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part180" data-index="130" style="margin-left:0;">Made more 70% ethanol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part181" data-index="131" style="margin-left:0;">Made sure pipette tips were everywhere </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part182" data-index="132" style="margin-left:0;">Cleaned all counters</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part183" data-index="133" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part184" data-index="134" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/21</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part185" data-index="135" style="margin-left:0;">Made CM and KAN plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part186" data-index="136" style="margin-left:0;">Made one liter of CM and one liter of KAN agar. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part187" data-index="137" style="margin-left:0;">The plates set out for two hours and were still not set up - they were left overnight but were still not set in the morning.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part188" data-index="138" style="margin-left:0;">All of the plates were discarded.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part189" data-index="139" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/22</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part190" data-index="140" style="margin-left:0;">Made CM and KAN plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part191" data-index="141" style="margin-left:0;">Made one liter of CM and one liter of KAN agar. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part192" data-index="142" style="margin-left:0;">Stored in the 4C fridge </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part193" data-index="143" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part194" data-index="144" style="margin-left:0;">Made DLF-00286 Competent Cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part195" data-index="145" style="margin-left:0;">Total of 29 aliquots of 50 uL were stored in the -80°C freezer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part196" data-index="146" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part197" data-index="147" style="margin-left:0;">Autoclaved Epi Tubes and washed glassware. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part198" data-index="148" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part199" data-index="149" style="margin-left:0;">Autoclaved glassware and biohazard trash.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part200" data-index="150" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part201" data-index="151" style="margin-left:0;">Made Electrocompetent Cells (E cloni)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part202" data-index="152" style="margin-left:35px;">9 aliquots were made</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/1</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part203" data-index="153" style="margin-left:0;">Made and autoclaved 1 L LB broth: transferred to autoclaved jar.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part204" data-index="154" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 7/6</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part205" data-index="155" style="margin-left:0;">Made Electrocompetent Cells (E cloni)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part206" data-index="156" style="margin-left:35px;">25 aliquots were made</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part207" data-index="157" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 8/27</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part208" data-index="158" style="margin-left:0;">Made Electrocompetent Cells (286 - J, R)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part209" data-index="159" style="margin-left:35px;">36 aliquots were made</div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div><div class="printable-page-container"><div class="protocolEditor"><div></div><div class="protocolEditor-pageContainer"><div class="protocolEditor-page"><div class="protocolEditor-contentWrapper"><h1 class="mediocre-titleEditor is-locked"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="ep223-title" data-index="0">LB Broth/Media Protocol</div></h1><div><h2>Introduction</h2><div class="intro-editor" data-editor="intro"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="true" data-cid="ep223-intro" data-index="0">LB Broth or Media is used to grow cells. Throughout the iGem Project, we used a low salt media because ...... The recipe below is for 1 L of Broth
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</div></div><h2>Materials</h2><div class="materials-editor" data-editor="materials"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><ul class="materials"><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m211" data-index="0">10 g Tryptone</div><ul class="components"></ul></li><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m212" data-index="1">5 g Yeast Extract</div><ul class="components"></ul></li><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m213" data-index="2">5 g Sodium Chloride (NaCl or salt)</div><ul class="components"><li class="component"><div class="component-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="c214" data-index="2,0">for a high salt media use 10 g of Sodium Chloride</div></li></ul></li><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m215" data-index="3">Deionized Water</div><ul class="components"></ul></li></ul></div><h2>Procedure</h2><div class="stepsEditor mediocre"><div class="editor" data-editor="steps"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="mediocre-item is-header"><ul class="headers"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s216" data-index="0">Make Broth</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s216" data-index="0"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="1"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s217" data-index="1">In a 2000 mL Erlenmeyer flask  mix the tryptone, yeast extract, sodium chloride.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s217" data-index="1"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="2"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s218" data-index="2">Use a graduated cylinder to measure out 1 L of deionized water </div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s218" data-index="2"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="3"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s219" data-index="3">Add the water to the flask and mix well</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s219" data-index="3"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="4"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s220" data-index="4">Autoclave on a liquid cycle for 15 min </div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s220" data-index="4"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-description"><ul class="descriptions"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s221" data-index="5">Make sure to add water or ice to the bucket before autoclaving.</div></div></li></ul></div><div class="mediocre-item is-description"><ul class="descriptions"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s222" data-index="6"></div></div></li></ul></div></div></div></div></div></div></div></div></div><div class="printable-page-container"><div class="protocolEditor"><div></div><div class="protocolEditor-pageContainer"><div class="protocolEditor-page"><div class="protocolEditor-contentWrapper"><h1 class="mediocre-titleEditor is-locked"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="ep258-title" data-index="0">LB Agar Plates</div></h1><div><h2>Introduction</h2><div class="intro-editor" data-editor="intro"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="true" data-cid="ep258-intro" data-index="0">This is a recipe for 1 L of LB for plates that are a low salt LB
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</div></div><h2>Materials</h2><div class="materials-editor" data-editor="materials"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><ul class="materials"><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m225" data-index="0">15 g Agar</div><ul class="components"></ul></li><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m226" data-index="1">10 g Tryptone</div><ul class="components"></ul></li><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m227" data-index="2">5 g Yeast Extract</div><ul class="components"></ul></li><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m228" data-index="3">5 g Sodium Chloride</div><ul class="components"><li class="component"><div class="component-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="c229" data-index="3,0">For a high salt agar, use 10 g of Sodium Chloride</div></li></ul></li><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m230" data-index="4">Deionized Water</div><ul class="components"></ul></li></ul></div><h2>Procedure</h2><div class="stepsEditor mediocre"><div class="editor" data-editor="steps"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="mediocre-item is-header"><ul class="headers"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s231" data-index="0">Media</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s231" data-index="0"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="1"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s232" data-index="1">In a 2000 mL Erlenmeyer Flask, add the agar, tryptone, yeast extract, and sodium chloride.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s232" data-index="1"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="2"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s233" data-index="2">In a graduated cylinder, measure out 1000 mL of deionized water.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s233" data-index="2"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="3"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s234" data-index="3">Add it to the flask and mix.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s234" data-index="3"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="4"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s235" data-index="4">Autoclave on a liquid cycle for 15 min</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s235" data-index="4"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-description"><ul class="descriptions"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s236" data-index="5">Make sure to add water or ice to the bin before autoclaving.</div></div></li></ul></div><div class="mediocre-item is-header"><ul class="headers"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s237" data-index="6">KAN (Kanamycin) Plates</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s237" data-index="6"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="5"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s238" data-index="7">Once out of the autoclave, allow media to cool.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s238" data-index="7"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="6"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s239" data-index="8">Add as many uL of KAN 1000X Stock as there is mL of media</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s239" data-index="8"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-description"><ul class="descriptions"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s240" data-index="9">For a 1 L media batch, add 1 mL of KAN 1000X stock.</div></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="7"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s241" data-index="10">Mix the flask well.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s241" data-index="10"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="8"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s242" data-index="11">Pour plates.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s242" data-index="11"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-description"><ul class="descriptions"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s243" data-index="12">Make sure the cover the entire bottom of the plate and remove as many bubbles as possible.</div></div></li></ul></div><div class="mediocre-item is-header"><ul class="headers"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s244" data-index="13">CM (Chloramphenicol) Plates</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s244" data-index="13"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="9"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s245" data-index="14">Once media is out of the autoclave, allow it to cool.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s245" data-index="14"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="10"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s246" data-index="15">Add as many uL of CM 1000X Stock as there is mL of media</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s246" data-index="15"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-description"><ul class="descriptions"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s247" data-index="16">For a 1 L media batch, add 1 mL of CM 1000X stock.</div></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="11"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s248" data-index="17">Mix the flask well.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s248" data-index="17"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="12"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s249" data-index="18">Pour plates.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s249" data-index="18"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-description"><ul class="descriptions"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s250" data-index="19">Make sure the cover the entire bottom of the plate and remove as many bubbles as possible.</div></div></li></ul></div><div class="mediocre-item is-header"><ul class="headers"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s251" data-index="20">AMP (Ampicillin) Plates</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s251" data-index="20"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="13"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s252" data-index="21">Once media is out of the autoclave, allow it to cool.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s252" data-index="21"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="14"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s253" data-index="22">Add as many uL of AMP 1000X Stock as there is mL of media</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s253" data-index="22"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-description"><ul class="descriptions"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s254" data-index="23">For a 1 L media batch, add 1 mL of AMP 1000X stock.</div></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="15"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s255" data-index="24">Mix the flask well.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s255" data-index="24"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="16"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s256" data-index="25">Pour plates.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s256" data-index="25"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-description"><ul class="descriptions"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s257" data-index="26">Make sure the cover the entire bottom of the plate and remove as many bubbles as possible.</div></div></li></ul></div></div></div></div></div></div></div></div></div><div class="printable-page-container"><div class="protocolEditor"><div></div><div class="protocolEditor-pageContainer"><div class="protocolEditor-page"><div class="protocolEditor-contentWrapper"><h1 class="mediocre-titleEditor is-locked"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="ep278-title" data-index="0">Glycerol Stocks</div></h1><div><h2>Introduction</h2><div class="intro-editor" data-editor="intro"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="true" data-cid="ep278-intro" data-index="0">Three concentrations of glycerol stocks.
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</div></div><h2>Materials</h2><div class="materials-editor" data-editor="materials"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><ul class="materials"><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m260" data-index="0">Glycerol </div><ul class="components"></ul></li><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m261" data-index="1">pico Water</div><ul class="components"><li class="component"><div class="component-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="c262" data-index="1,0">Autoclaved deionized water</div></li></ul></li></ul></div><h2>Procedure</h2><div class="stepsEditor mediocre"><div class="editor" data-editor="steps"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="mediocre-item is-header"><ul class="headers"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s263" data-index="0"><span style="color:#000000">10% Glycerol Stock</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s263" data-index="0"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="1"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s264" data-index="1"><span style="color:#000000">Add 50 mL of 100% glycerol to liter bottle</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s264" data-index="1"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="2"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s265" data-index="2"><span style="color:#000000">Fill bottle to 500 mL with pH2O</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s265" data-index="2"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="3"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s266" data-index="3"><span style="color:#000000">Autoclave on liquid cycle for 15 min</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s266" data-index="3"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="4"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s267" data-index="4"><span style="color:#000000">Store at room temp</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s267" data-index="4"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-header"><ul class="headers"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s268" data-index="5"><span style="color:#000000">50% Glycerol Stock</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s268" data-index="5"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="5"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s269" data-index="6"><span style="color:#000000">Add 250 mL of 100% glycerol to liter bottle</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s269" data-index="6"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="6"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s270" data-index="7"><span style="color:#000000">Fill bottle to 500 mL with pH2O</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s270" data-index="7"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="7"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s271" data-index="8"><span style="color:#000000">Autoclave on liquid cycle for 15 min</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s271" data-index="8"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="8"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s272" data-index="9"><span style="color:#000000">Store at room temp</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s272" data-index="9"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-header"><ul class="headers"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s273" data-index="10"><span style="color:#000000">Made 20% Glycerol Stocks</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s273" data-index="10"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="9"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s274" data-index="11"><span style="color:#000000">Add 40 mL of 100% Glycerol  to a 500 mL bottle</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s274" data-index="11"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="10"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s275" data-index="12"><span style="color:#000000"> Add 160 mL of pH2O.</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s275" data-index="12"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="11"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s276" data-index="13">Autoclave on liquid cycle for 15 min.</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s276" data-index="13"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="12"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s277" data-index="14">Store at room temp</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s277" data-index="14"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div></div></div></div></div></div></div></div></div><div class="printable-page-container"><div class="protocolEditor"><div></div><div class="protocolEditor-pageContainer"><div class="protocolEditor-page"><div class="protocolEditor-contentWrapper"><h1 class="mediocre-titleEditor is-locked"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="ep301-title" data-index="0">Antibiotic Stocks</div></h1><div><h2>Introduction</h2><div class="intro-editor" data-editor="intro"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="true" data-cid="ep301-intro" data-index="0">Three kinds of antibiotic stocks (1000x). These recipes make 1000X stocks that follow the recommendations for plates.
 +
 +
</div></div><h2>Materials</h2><div class="materials-editor" data-editor="materials"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><ul class="materials"><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m280" data-index="0">Antibiotic Powder</div><ul class="components"><li class="component"><div class="component-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="c281" data-index="0,0">Stored in -20 C freezer</div></li></ul></li><li class="material"><div class="material-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="m282" data-index="1">Pico Water</div><ul class="components"></ul></li></ul></div><h2>Procedure</h2><div class="stepsEditor mediocre"><div class="editor" data-editor="steps"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="mediocre-item is-header"><ul class="headers"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s283" data-index="0">Kanamycin Stocks (10 mL)</div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s283" data-index="0"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="1"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s284" data-index="1"><span style="color:#000000">Use 10 mL of pico water (autoclaved dH2O)</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s284" data-index="1"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="2"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s285" data-index="2"><span style="color:#000000">Add 500 mg of Kanamycin powder (-20 C freezer)</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s285" data-index="2"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="3"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s286" data-index="3"><span style="color:#000000">Mix well</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s286" data-index="3"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="4"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s287" data-index="4"><span style="color:#000000">Filter [using antibiotic filters and syringes (in drawer underneath middle bench)] into aliquots of 1 ml epi tubes</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s287" data-index="4"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="5"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s288" data-index="5"><span style="color:#000000">Store aliquots labeled with concentration (1000x), date, and KAN in -20 C freezer</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s288" data-index="5"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-header"><ul class="headers"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s289" data-index="6"><span style="color:#000000">Ampicillin Stock (10 mL)</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s289" data-index="6"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="6"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s290" data-index="7"><span style="color:#000000">Use 10 mL of pico water (autoclaved dH2O)</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s290" data-index="7"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="7"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s291" data-index="8"><span style="color:#000000">Add 1 g of Ampicillin powder (-20 C freezer)</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s291" data-index="8"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="8"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s292" data-index="9"><span style="color:#000000">Mix well</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s292" data-index="9"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="9"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s293" data-index="10"><span style="color:#000000">Filter using antibiotic filters into 1 mL aliquots (epi tubes)</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s293" data-index="10"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="10"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s294" data-index="11"><span style="color:#000000">Store aliquots labeled with concentration (1000x), date, and AMP in -20 C freezer</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s294" data-index="11"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-header"><ul class="headers"><li><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s295" data-index="12"><span style="color:#000000">Chloramphenicol Stock (10 mL)</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s295" data-index="12"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ul></div><div class="mediocre-item is-step"><ol class="steps"><li value="11"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s296" data-index="13"><span style="color:#000000">Use 10 mL of ethanol</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s296" data-index="13"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="12"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s297" data-index="14"><span style="color:#000000">Add 250 mg of Chloramphenicol  powder (-20 C freezer)</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s297" data-index="14"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="13"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s298" data-index="15"><span style="color:#000000">Mix well</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s298" data-index="15"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="14"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s299" data-index="16"><span style="color:#000000">Filter using antibiotic filters into 1 mL aliquots (epi tubes)</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s299" data-index="16"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div><div class="mediocre-item is-step"><ol class="steps"><li value="15"><div class="step-checkbox"><span class="glyphicon glyphicon-ok" icon="ok"></span></div><div class="step-editable-wrapper"><div class="step-editable multi-editable editable selectable is-locked" contenteditable="false" data-cid="s300" data-index="17"><span style="color:#000000">Store aliquots labeled with concentration (1000x), date, and CM in -20 C freezer</span></div></div><div class="time-editable-wrapper"><div class="time-editable force-visible editable selectable is-locked" contenteditable="false" data-cid="time-s300" data-index="17"></div><span class="glyphicon glyphicon-time time-editable-wrapper-icon" icon="time"></span></div></li></ol></div></div></div></div></div></div></div></div></div></div></div></div>
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<div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Creating Parts</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> Duke iGEM 2016</div><div><b>Authors:</b> Jaydeep Sambangi</div><div><b>Dates:</b> 2016-05-18 to 2016-07-28</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 5/18/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part413" data-index="0" style="margin-left:0;">iCultures Grown:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part414" data-index="1" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part415" data-index="2" style="margin-left:35px;">pSMART-yibDp-GFPuv</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part416" data-index="3" style="margin-left:70px;">"GFP" or "yib" </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part417" data-index="4" style="margin-left:35px;">pSMART-EV</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part418" data-index="5" style="margin-left:70px;">"pSMART" or "PSM"</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part419" data-index="6" style="margin-left:35px;">DLF-00286</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part420" data-index="7" style="margin-left:70px;">"286"</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part421" data-index="8" style="margin-left:35px;">E-cloni</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part422" data-index="9" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part423" data-index="10" style="margin-left:0;">Results:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part424" data-index="11" style="margin-left:0;"><span style="color:#D10E0E">(5/19)</span><span style="color:#DE5555"> All 8 Cultures grew successfully </span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part425" data-index="12" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part426" data-index="13" style="margin-left:0;">Notes:</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part427-item" data-formattable="true" data-index="14" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Taxol_G-Blocks.docx</div></h5><div class=""></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part427" data-index="14">
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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 5/19/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part429" data-index="15" style="margin-left:0;">Miniprep pSMART and yib </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part430" data-index="16" style="margin-left:0;">Used a 30 mL elution </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part431" data-index="17" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part432" data-index="18" style="margin-left:0;">Tested the concentration using UV/Absorbance and found that at 280 nm, the tib had an absorbance of 0.006 and at 260 nm it had an absorbance of 0.019. Using the ratio of Abs 1= 50 ug/mL this leads to the tib miniprep resulting in DNA of a concentration 95 ug/mL. The pSMART had an absorbance of 0.0037 at 260 nm and an absorbance of 0.025 at 280 nm. the ratio of 260:280 of 1.48. This leads to a concentration of 135 ug/mL. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part433" data-index="19" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part434" data-index="20" style="margin-left:0;">Protocols:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part435" data-index="21" style="margin-left:35px;">Miniprep Protocol NEB</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part436" data-index="22" style="margin-left:35px;">Testing Miniprep Concentration</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part437" data-index="23" style="margin-left:0;">Gibsons</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part438" data-index="24" style="margin-left:0;">Performed Gibson Assemblies on:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part439" data-index="25" style="margin-left:35px;">BadA (Nisa)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part440" data-index="26" style="margin-left:35px;">PAM (Thomas)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part441" data-index="27" style="margin-left:35px;">TAT (Parth)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part442" data-index="28" style="margin-left:35px;">TBT (Adam)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part443" data-index="29" style="margin-left:35px;">BAPT (Adam)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part444" data-index="30" style="margin-left:35px;">TAX10 (Emma)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part445" data-index="31" style="margin-left:35px;">DBAT (Ben)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part446" data-index="32" style="margin-left:35px;">TycA (Parth)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part447" data-index="33" style="margin-left:0;">Used recipes below</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part448-item" data-formattable="true" data-index="34" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:366px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">GibsonPlan_-_Bootcamp_20160519.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="jALvz3flpGRj4dhqN2dQMXTKP1tt7qTsh4I6IJkj-GibsonPlan_-_Bootcamp_20160519.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part448" data-index="34">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part450" data-index="35" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part451" data-index="36" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part452" data-index="37" style="margin-left:35px;">Gibson Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part453" data-index="38" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part454" data-index="39" style="margin-left:0;">Prepared Electrocompetent Cells (50 mL) of DLF-00286 and E-cloni</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part455" data-index="40" style="margin-left:0;">No changes from protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part456" data-index="41" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part457" data-index="42" style="margin-left:35px;">Electrocompetent Cells Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part458" data-index="43" style="margin-left:0;">Made Freezer Stocks</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part459" data-index="44" style="margin-left:0;">4 stocks were made of each strain for now; 4 stocks were made as a back up</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part460" data-index="45" style="margin-left:0;">Used 50% instead of 20% Glycerol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part461" data-index="46" style="margin-left:0;">4 Strains were:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part462" data-index="47" style="margin-left:35px;">yib</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part463" data-index="48" style="margin-left:35px;">pSMART</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part464" data-index="49" style="margin-left:35px;">286</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part465" data-index="50" style="margin-left:35px;">Ecloni</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part466" data-index="51" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part467" data-index="52" style="margin-left:35px;">Freezer Stock Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part468" data-index="53" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part469" data-index="54" style="margin-left:0;">Transformations of Gibsons</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part470" data-index="55" style="margin-left:0;">Used electroporation to transform gibsons into competent cells.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part471" data-index="56" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part472" data-index="57" style="margin-left:35px;">Electroporation Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part473" data-index="58" style="margin-left:0;">Used 50 uL of Competent Cells and 1 uL of DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part474" data-index="59" style="margin-left:0;">Plated the transformations after a one hour recovery.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part475" data-index="60" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part476" data-index="61" style="margin-left:0;">Results:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part477" data-index="62" style="margin-left:0;"><span style="color:#D10E0E">(5/20)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part478" data-index="63" style="margin-left:0;"><span style="color:#DE5555">[Name], [10uL colony #], [200uL colony #]</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part479" data-index="64" style="margin-left:70px;"><span style="color:#DE5555">badA, 1, 6</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part480" data-index="65" style="margin-left:70px;"><span style="color:#DE5555">PAM, 0, 0</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part481" data-index="66" style="margin-left:70px;"><span style="color:#DE5555">TAT, 0, 28</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part482" data-index="67" style="margin-left:70px;"><span style="color:#DE5555">TBT, 0, 0</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part483" data-index="68" style="margin-left:70px;"><span style="color:#DE5555">BAPT, 23, Lots</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part484" data-index="69" style="margin-left:70px;"><span style="color:#DE5555">TAX10, 2, 20</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part485" data-index="70" style="margin-left:70px;"><span style="color:#DE5555">DBAT, 4, 55</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part486" data-index="71" style="margin-left:70px;"><span style="color:#DE5555">TycA, 0, 0</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part487" data-index="72" style="margin-left:70px;"><span style="color:#DE5555">Control, 0</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part488" data-index="73" style="margin-left:35px;"><span style="color:#DE5555">Calculating Efficiency</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part489" data-index="74" style="margin-left:0;"><span style="color:#DE5555">CFU/ug = [(Colony #)* (Dilution)*(1000 ng/ug)] / X (ng/L) = 4.4✕107 CFU/ug for E-cloni</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part490" data-index="75" style="margin-left:70px;"><span style="color:#DE5555">CFU = “Colony Forming Unit”</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part491" data-index="76" style="margin-left:0;">Notes:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part492" data-index="77" style="margin-left:0;">Possible Projects include:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part493" data-index="78" style="margin-left:35px;"><span style="color:#000000">Cloning hydroxylase enzymes (x7)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part494" data-index="79" style="margin-left:35px;"><span style="color:#000000">Fermenting Taxadiene (cannot buy)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part495" data-index="80" style="margin-left:35px;"><span style="color:#000000">Clone 10 genes in Taxol pathway, map how it works (currently unknown)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part496" data-index="81" style="margin-left:35px;"><span style="color:#000000">Enzyme Engineering - improve enzymes</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part497" data-index="82" style="margin-left:35px;"><span style="color:#000000">NEB videos</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part498" data-index="83" style="margin-left:35px;"><span style="color:#000000">Computation for CRISPR</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part499" data-index="84" style="margin-left:35px;"><span style="color:#000000">Take pictures to document summer work</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part500" data-index="85" style="margin-left:35px;"><span style="color:#000000">Educational Outreach</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part501" data-index="86" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 5/20/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part502" data-index="87" style="margin-left:0;">Colony PCR (Emma; Thomas; Adam; Nisa; Parth)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part503" data-index="88" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part504" data-index="89" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part505" data-index="90" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part506-item" data-formattable="true" data-index="91" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="91" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part506-table" data-formattable="true" data-index="91" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part506-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part506-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part506-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part506-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part506-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part506-1-2" data-namespace="table-ns5179"><span>27</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part506-2-0" data-namespace="table-ns5179"><span>MM</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part506-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part506-2-2" data-namespace="table-ns5179"><span>337.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part506-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part506-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part506-3-2" data-namespace="table-ns5179"><span>6.75</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part506-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part506-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part506-4-2" data-namespace="table-ns5179"><span>6.75</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part506-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part506-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part506-5-2" data-namespace="table-ns5179"><span>324</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table1</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part506" data-index="91" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part507" data-index="92" style="margin-left:0;">Ran Gel on Colony PCR</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part508" data-index="93" style="margin-left:0;"><span style="color:#000000">No samples worked</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part509" data-index="94" style="margin-left:0;"><span style="color:#000000">Only GFP and pSMART had bands present</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part510" data-index="95" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 5/23/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part511" data-index="96" style="margin-left:0;">Gibson Assembly</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part512" data-index="97" style="margin-left:0;">Performed the Gibson again with a new recipe - less vector and more water (ran out of vector)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part513" data-index="98" style="margin-left:0;">Used 1 uL of vector; 5 uL of mix, and 1.5 uL of inserts </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part514" data-index="99" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part515-item" data-formattable="true" data-index="100" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Gibson_Plan_for_5_23_.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="cHD4M36sTLjkx100J7XGNUo9uS0o3kVY84YDi6MI-Gibson_Plan_for_5_23_.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part515" data-index="100">
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part517" data-index="101" style="margin-left:0;">Transformations of Gibsons</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part518" data-index="102" style="margin-left:0;">Used 3 uL of DNA; 20 uL of Comp Cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part519" data-index="103" style="margin-left:0;">Recovered for 2-3 hours </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part520" data-index="104" style="margin-left:0;">All samples electroporated for 654 ms at around 1600 V</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part521" data-index="105" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part522" data-index="106" style="margin-left:0;">Plated Transformation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part523" data-index="107" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part524-item" data-formattable="true" data-index="108" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:344px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">PAM_Transformed_5_23_2016.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="M8wxYMGHRxp1L91nVgbVSAfVF2qSP1hsXcR3fpJa-PAN_Transformed_5_23_2016.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part524" data-index="108">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part526" data-index="109" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part527-item" data-formattable="true" data-index="110" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:341px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">TAT_Transformed_5_23_2016.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="d3oAsyfkYbq4xvt7BJXujEOplFTw0fiRnbtPGEEb-TAT_Transformed_5_23_2016.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part527" data-index="110">
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part529-item" data-formattable="true" data-index="111" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:338px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">TAX10_Transformed_5_23_2016.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="VkofqaEtY97gJcKVeHor7ObfPybFPmxilFUwwBQQ-TAX10_Transformed_5_23_2016.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part529" data-index="111">
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part531-item" data-formattable="true" data-index="112" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:338px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">TBT_Transformed_5_23_2016.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="VbBoejFlGj5ZOkypYb71hZUmG28Kq8X2qUNyhXKA-TBT_Transformed_5_23_2016.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part531" data-index="112">
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part533-item" data-formattable="true" data-index="113" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:335px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">TycA_Transformed_5_23_2016.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="vk7WfTidPVvLEpbGHF4y9tM0wmry9QD0S7ny4sWd-TycA_Transformed_5_23_2016.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part533" data-index="113">
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part535-item" data-formattable="true" data-index="114" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:335px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">BAPT_Transformed_5_23_2016.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="uVHWXx5RbEBjETokAsZqHXD1NexKf9PGnDgkFCdK-BAPT_Transformed_5_23_2016.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part535" data-index="114">
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part537-item" data-formattable="true" data-index="115" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:335px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">DBAT_Transformed_5_23_2016.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="CA9bsHebxT6TItfJzJKWqZ2oqTmwWQe3TV0BKPZF-DBAT_Transformed_5_23_2016.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part537" data-index="115">
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part539" data-index="116" style="margin-left:0;">BadA did not grow any colonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part540" data-index="117" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part541" data-index="118" style="margin-left:0;">Colony PCR of Old Plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part542" data-index="119" style="margin-left:0;">Plates from 5/20/2016</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part543" data-index="120" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part544" data-index="121" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part545-item" data-formattable="true" data-index="122" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="122" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part545-table" data-formattable="true" data-index="122" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part545-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part545-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part545-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part545-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part545-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part545-1-2" data-namespace="table-ns5179"><span>16</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part545-2-0" data-namespace="table-ns5179"><span>MM</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part545-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part545-2-2" data-namespace="table-ns5179"><span>200</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part545-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part545-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part545-3-2" data-namespace="table-ns5179"><span>10</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part545-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part545-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part545-4-2" data-namespace="table-ns5179"><span>10</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part545-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part545-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part545-5-2" data-namespace="table-ns5179"><span>200</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table2</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part545" data-index="122" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part546" data-index="123" style="margin-left:0;">Inoculation of E. cloni (Parth; Nisa)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part547" data-index="124" style="margin-left:0;">Inoculated a colony of E. cloni in 35 uL of LB broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part548" data-index="125" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 5/24/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part549" data-index="126" style="margin-left:0;">Ran Gels on 5/19 Gibsons</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part550" data-index="127" style="margin-left:0;">8 ul of ladder; 2 ul of DNA stain; 10 ul of DNA; 160 V for 20 min</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part551-item" data-formattable="true" data-index="128" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:469px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">524_PCR_Colony_Screening_Gel.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="2iTCqwXCuqaXpef8ikcLU0Cfo7MrG0lx1iP4WN5t-524_PCR_Colony_Screening_Gel.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part551" data-index="128">1: Ladder
 +
2: Positive Control
 +
3: Negative Control
 +
4: tycA
 +
5: DBAT
 +
6: TAX10
 +
7: BAPT
 +
8: Ladder
 +
9: TBT
 +
10: TAT
 +
11: PAM
 +
12: BadA
 +
13: Empty
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part553" data-index="129" style="margin-left:0;">There were no discernible bands; results do not align with expected transformation success</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part554" data-index="130" style="margin-left:0;">Cause is unknown as there were numerous colonies on plate but no inserts detectd</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part555" data-index="131" style="margin-left:0;"><span style="color:#000000">Because the mini prepped positive control clearly worked, hypothesis is that the econoTaq master mix is not successfully breaking down the cells to allow the DNA inside the nucleus to replicate.</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part556" data-index="132" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part557" data-index="133" style="margin-left:0;">Colony PCR of Gibsons from 5/23 (Ben; Emma)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part558" data-index="134" style="margin-left:0;">8 colonies were celected from each plate except BadA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part559" data-index="135" style="margin-left:0;">Made LB tubes with 50 mL of KAN + LB</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part560" data-index="136" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part561" data-index="137" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part562" data-index="138" style="margin-left:0;">Transformation of badA into Comp Cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part563" data-index="139" style="margin-left:0;">Used badA Gibson from 5/23 and E. cloni from 5/24</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part564" data-index="140" style="margin-left:0;">Separated Gibson into three different transformations (T1, T2, and T3)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part565" data-index="141" style="margin-left:0;">Recovered for 3 hours before plating onto KAN plates </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part566" data-index="142" style="margin-left:35px;">Left overnight in incubator at 37 C</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part567" data-index="143" style="margin-left:0;">Electroporation Results:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part568" data-index="144" style="margin-left:35px;">T1: 250 V; 654 ms</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part569" data-index="145" style="margin-left:35px;">T2: 1690 V; 654 ms</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part570" data-index="146" style="margin-left:35px;">T3: 660 V; 654 ms</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part571" data-index="147" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 5/25/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part572" data-index="148" style="margin-left:0;">Ran Gels for Colony PCR from 5/24</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part573" data-index="149" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part574" data-index="150" style="margin-left:35px;">Gels</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part575" data-index="151" style="margin-left:0;">Used 10 uL of DNA instead of 5 uL of H20 and 5 uL of DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part576" data-index="152" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part577" data-index="153" style="margin-left:0;"><span style="color:#000000">The Controls were diluted with 7uL of H2O in order to have enough to use in every gel (there was only 25 uL available after the PCR). </span><span style="color:#000000">The controls were also diluted by H2O in each well (5 uL of DNA and 5 uL of H2O). </span><span style="color:#000000">Controls were yibDp-GFP (+) and pSmart, no insert (-)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part578" data-index="154" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part579" data-index="155" style="margin-left:0;">Two sets of three Gels ran for 35 min at 160 V</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part580" data-index="156" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part581" data-index="157" style="margin-left:0;"><span style="color:#FF0000">Note: Controls seemed to be the flipped version of what was expected. Assumed that controls were mislabeled</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part582" data-index="158" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part583" data-index="159" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part584-item" data-formattable="true" data-index="160" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">05252016_Angelica1.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="P8moaIATLP6fCytVBDuvUweAIIo1QdBKmGFPSt1B-5-25-2016_Angelica.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part584" data-index="160"><span style="color:#5C7C83"><span style="background-color:#FFFFFF">1: Ladder
 +
2: Negative Control
 +
3: Positive Control
 +
4-11: PAM 1-8
 +
12-13: BAPT 3-4</span></span>
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part586-item" data-formattable="true" data-index="161" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">05252016_Brandon1.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="tl8hE9N2wdhqzxK71jj7CjlPuy0o8K0OuEXgMqU4-5-25-16_Brandon.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part586" data-index="161">1: Ladder
 +
2: Positive Control
 +
3: Negative Control
 +
4-11: DBAT 1-8
 +
12-13: BAPT 1-2
 +
 +
*DBAT 2 (well 5) was a poor band length; less sample was added
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part588-item" data-formattable="true" data-index="162" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">05252016_Suzie1.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="wAcrevbf5FZVlOjQLmCRQvRhiw4OfIUTRrYxRiE7-5-25-16_Suzie.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part588" data-index="162">1: Ladder
 +
2: Positive Control
 +
3: Negative Control
 +
4-11: TAT 1-8
 +
12-13: BAPT 5-6
 +
 +
*TAT 3-6 (wells 6-9) had bands too small
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part590-item" data-formattable="true" data-index="163" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">05252016_Angelica2.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="nh2J521iiYBqU6PY0VO0ISp4y0PTemd0qQ3NjEgL-05252016_Angelica2.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part590" data-index="163"><span style="color:#5C7C83"><span style="background-color:#FFFFFF">1: Ladder
 +
2: Negative Control
 +
3: Positive Control
 +
4-11: TBT 1-8
 +
12-13: BAPT 7-8</span></span>
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part592-item" data-formattable="true" data-index="164" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">05252016_Brandon2.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="obRXyNp5r0wbsoGuWR4MDB1BX4AlR9v0Rz6jlLvS-05252016_Brandon2.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part592" data-index="164">1: Ladder
 +
2: Negative Control
 +
3-10: TAX10 1-8
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part594-item" data-formattable="true" data-index="165" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">05252016_Suzie2.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="C7hdlTXfYhSbhiCZT6SD2PFzMG1vj9fT6NbM8yaW-05252016_Suzie2.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part594" data-index="165"><span style="color:#5C7C83"><span style="background-color:#FFFFFF">1: Ladder
 +
2: Negative Control
 +
3-10: TycA 1-8</span></span>
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part596" data-index="166" style="margin-left:0;">DNA PCR (Emma; Thomas)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part597" data-index="167" style="margin-left:0;">Goal: To determine if the Gibsons worked; Is the Colony PCR lysing the cells enough?</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part598" data-index="168" style="margin-left:0;">Used Colony PCR protocol but added 1 uL of DNA instead of 1 colony</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part599" data-index="169" style="margin-left:0;">For BadA, 2 uL of dH2O was added to the gibson and the entire volume added to the PCR tube</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part600" data-index="170" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part601" data-index="171" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part602-item" data-formattable="true" data-index="172" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="172" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part602-table" data-formattable="true" data-index="172" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part602-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part602-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part602-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part602-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part602-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part602-1-2" data-namespace="table-ns5179"><span>10</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part602-2-0" data-namespace="table-ns5179"><span>MM</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part602-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part602-2-2" data-namespace="table-ns5179"><span>125</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part602-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part602-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part602-3-2" data-namespace="table-ns5179"><span>2.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part602-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part602-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part602-4-2" data-namespace="table-ns5179"><span>2.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part602-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part602-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part602-5-2" data-namespace="table-ns5179"><span>120</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table3</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part602" data-index="172" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part603" data-index="173" style="margin-left:0;">Ran Gel</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part604-item" data-formattable="true" data-index="174" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:412px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">05252016_PCRAmplificationofGibsonReactions.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Qs3GvCA109tXSfr1FpHaFi10OSkjGYdrpBJW81yS-5-25_PCR_Amplification_of_Gibson_Reactions_.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part604" data-index="174">1: Ladder
 +
2: pSMART
 +
3: badA
 +
4: PAM
 +
5: TAT
 +
6: TBT
 +
7: Empty
 +
8: BAPT
 +
9: TAX10
 +
10: DBAT
 +
11: TycA
 +
12-13: Empty
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part606" data-index="175" style="margin-left:0;">No insert bands appeared </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part607" data-index="176" style="margin-left:0;">Suspected that the yib and pSMART labels were switched as the control was a pSMART and not the intended yib; labeling has been changed on tubes and on the picture.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part608" data-index="177" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 5/26/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part609" data-index="178" style="margin-left:0;">Econo Taq PCR (Ben)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part610" data-index="179" style="margin-left:0;"><span style="color:#000000">Goal: Test YibDp and pSmart in freezer to see if they are mislabeled; Is there a better Master Mix Recipe?</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part611" data-index="180" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part612" data-index="181" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part613" data-index="182" style="margin-left:0;"><span style="color:#000000">Two Master Mixes were made </span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part614" data-index="183" style="margin-left:35px;"><span style="color:#000000">MM1 used the protocol given us by Charlie (0.25uL of each primer). </span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part615" data-index="184" style="margin-left:35px;"><span style="color:#000000">MM2 used the protocol Adam remembers (1.25uL of each primer in each tube). </span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part616" data-index="185" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part617" data-index="186" style="margin-left:0;"><span style="color:#000000">The YibDp and the pSmart in the freezer were run on the PCR machine under each of these MM conditions. The mixes are given below:</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part618" data-index="187" style="margin-left:35px;"><span style="color:#000000">Added  25uL EconoTaq / .5uL SL1 / .5uL SR2 / 24uL pH2O to Master Mix 1. 23uL of this was added to two tubes. 1.5uL DNA added after.</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part619" data-index="188" style="margin-left:70px;"><span style="color:#000000">Each Reaction: 12.5uL EconoTaq / .25uL each primer / 12uL pH2O / Total = 25uL - 2uL to account for potential pipetting error</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part620" data-index="189" style="margin-left:35px;"><span style="color:#000000">Added 25uL EconoTaq / 2.5uL SL1 / 2.5uL SR2 / 20uL pH2O to Master Mix 2. 23uL of this was added to two tubes. 1.5uL DNA added after.</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part621" data-index="190" style="margin-left:70px;"><span style="color:#000000">Each Reaction: 12.5uL EconoTaq / 1.25uL each primer / 10uL pH2O / Total = 25uL - 2uL to account for potential pipetting error.</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part622" data-index="191" style="margin-left:35px;"><span style="color:#000000">These were run on the same PCR protocol as yesterday. (98C for 5min, then cycle x35: (98C for 30s) / (50C for 30s) / (72C for 3min)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part623" data-index="192" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part624" data-index="193" style="margin-left:0;"><span style="color:#000000">Note: 3min is overkill… Whoops; specifically, the increased extension time is unnecessary due to our insert being ~1kb</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part625" data-index="194" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part626" data-index="195" style="margin-left:0;">The PCR was then ran on a gel</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part627" data-index="196" style="margin-left:0;"><span style="color:#000000">Expectation:</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part628" data-index="197" style="margin-left:70px;"><span style="color:#000000">1kb band in both yibDp-GFPs, garbage in pSmart (since there is no insert)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part629" data-index="198" style="margin-left:70px;"><span style="color:#000000">If the results of the samples seem flipped, then the minipreps of the samples located in the fridge have their labels swapped</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part630" data-index="199" style="margin-left:70px;"><span style="color:#000000">If the samples with 1.25 μL of primer are clearer, then the PCR protocol will be updated with this improvement</span></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part631-item" data-formattable="true" data-index="200" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">05/26/2016 psmart vs yibd.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="WLJ75icA0ihlWsGeI5AXdIdrPx5oZV6yIMQLfLt8-5-26_psmart_vs_yibd.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part631" data-index="200">1: Ladder
 +
2: yibDp-GFP *
 +
3: pSMART *
 +
4: Empty
 +
5: <span style="color:#5C7C83"><span style="background-color:#FFFFFF">yibDp-GFP **
 +
6: pSMART **</span></span>
 +
 +
*0.25 uL of primer
 +
** 1.25 uL of primer
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part633" data-index="201" style="margin-left:0;"><span style="color:#DE5555">The bands indicate that the minipreps had their labels swapped.</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part634" data-index="202" style="margin-left:0;"><span style="color:#DE5555">PCR machine is proven effective at doing PCR. </span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part635" data-index="203" style="margin-left:0;"><span style="color:#DE5555">0.25 uL of primer is adequate for good PCR results.</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part636" data-index="204" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part637" data-index="205" style="margin-left:0;">Inoculations (Ben)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part638" data-index="206" style="margin-left:0;"><span style="color:#000000">The following culture samples were inoculated from single colony plates (2 colonies per plate) into 5mL of LB+Kan. The numbers were marked with the corresponding colonies on the plates:</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part639" data-index="207" style="margin-left:35px;"><span style="color:#000000">BAPT-1</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part640" data-index="208" style="margin-left:35px;"><span style="color:#000000">BAPT-2</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part641" data-index="209" style="margin-left:35px;"><span style="color:#000000">DBAT-1</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part642" data-index="210" style="margin-left:35px;"><span style="color:#000000">DBAT-2</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part643" data-index="211" style="margin-left:35px;"><span style="color:#000000">TAT-1</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part644" data-index="212" style="margin-left:35px;"><span style="color:#000000">TAT-2</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part645" data-index="213" style="margin-left:35px;"><span style="color:#000000">TycA-1</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part646" data-index="214" style="margin-left:35px;"><span style="color:#000000">TycA-2</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part647" data-index="215" style="margin-left:35px;"><span style="color:#000000">PAM-1</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part648" data-index="216" style="margin-left:35px;"><span style="color:#000000">PAM-2</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part649" data-index="217" style="margin-left:35px;"><span style="color:#000000">TBT-1</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part650" data-index="218" style="margin-left:35px;"><span style="color:#000000">TBT-2</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part651" data-index="219" style="margin-left:35px;"><span style="color:#000000">BadA-1</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part652" data-index="220" style="margin-left:35px;"><span style="color:#000000">BadA-2</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part653" data-index="221" style="margin-left:35px;"><span style="color:#000000">TAX10-1</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part654" data-index="222" style="margin-left:35px;"><span style="color:#000000">TAX10-2</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part655" data-index="223" style="margin-left:0;"><span style="color:#FF0000">5/27 </span><span style="color:#FF0000">All cultures grew except for BadA-1</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part656" data-index="224" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 5/27/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part657" data-index="225" style="margin-left:0;">Freezer Stocks/Miniprep/Concentration Check of Inoculations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part658" data-index="226" style="margin-left:0;">Protocols:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part659" data-index="227" style="margin-left:35px;">Freezer Stocks Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part660" data-index="228" style="margin-left:35px;">Miniprep Protocol NEB</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part661" data-index="229" style="margin-left:35px;">Testing Miniprep Concentrations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part662" data-index="230" style="margin-left:0;">Freezer Stock Edit: Used 500 uL of glycerol and culture instead of 750 uL. Stocks were created for each inoculation from 5/26.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part663" data-index="231" style="margin-left:0;">Miniprep Edit: Used a 50 uL elution. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part664" data-index="232" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part665-item" data-formattable="true" data-index="233" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="233" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part665-table" data-formattable="true" data-index="233" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:568px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:548px;"><colgroup><col style="width:45px;"></col><col style="width:63px;"></col><col style="width:51px;"></col><col style="width:54px;"></col><col style="width:50px;"></col><col style="width:52px;"></col><col style="width:61px;"></col><col style="width:54px;"></col><col style="width:57px;"></col><col style="width:61px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">H</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">I</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample:</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>PAM-1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-0-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>PAM-2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-0-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>DBAT-1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-0-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>DBAT-2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-0-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>TBT-1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-0-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>TBT-2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-0-7" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10-1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-0-8" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10-2</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>ng/uL:</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>11</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-1-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>300</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-1-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>88</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-1-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>94</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-1-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>57</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-1-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>175</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-1-7" data-namespace="table-ns5179" style="color:#DE5555;"><span>89</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-1-8" data-namespace="table-ns5179" style="color:#DE5555;"><span>96</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-2-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample:</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-2-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT-1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-2-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT-2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-2-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA-1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-2-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA-2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-2-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>BadA-1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-2-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>BadA-2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-2-7" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAT-1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-2-8" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAT-2</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part665-3-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>ng/uL:</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-3-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>101</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-3-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>63</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-3-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>11</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-3-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>48</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-3-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-3-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>146</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-3-7" data-namespace="table-ns5179" style="color:#DE5555;"><span>59</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part665-3-8" data-namespace="table-ns5179" style="color:#DE5555;"><span>119</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table4</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part665" data-index="233" style="width:568px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part666" data-index="234" style="margin-left:0;">PCR Mini prepped DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part667" data-index="235" style="margin-left:0;">Used a colony PCR protocol </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part668" data-index="236" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part669" data-index="237" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part670" data-index="238" style="margin-left:0;">Due to concentration results and limited quantity of Econo Taq, PAM-1, TycA-1, and BadA-1 were not run</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part671" data-index="239" style="margin-left:0;">Used 0.28 uL of each primer instead of 0.25 uL </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part672" data-index="240" style="margin-left:0;">Used 1 ng/uL dilutions of the mini prepped DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part673" data-index="241" style="margin-left:35px;">Dilutions were made is small epi tubes and stored in the -20 C freezer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part674" data-index="242" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part675" data-index="243" style="margin-left:0;">Gel was run on the PCR Results</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part676" data-index="244" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part677" data-index="245" style="margin-left:35px;">Gels</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part678" data-index="246" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part679-item" data-formattable="true" data-index="247" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:462px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">5-27-16_Angelica (1).jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ikvp3UAaOvzeM0QIAZZyVdXnrZuf0dfOWMDbRRiH-5-27-16_Angelica_1.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part679" data-index="247"><span style="color:#5C7C83"><span style="background-color:#FFFFFF">1: Ladder
 +
2: BadA-2
 +
3: PAM-2
 +
4: TAT-1</span></span>
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">5: TAT-2</span></span><span style="color:#5C7C83"><span style="background-color:#FFFFFF"> </span></span>
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">6: TBT-1</span></span>
 +
7: TBT-2
 +
8: BAPT-1
 +
8.5: Yib-pD
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part681-item" data-formattable="true" data-index="248" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:462px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">5-27-16_Brandon (1).jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="vFfQgRXnYE68F9G55i6yHT6xQdQKronS7kqjSMMy-5-27-16_Brandon_1.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part681" data-index="248"><span style="color:#5C7C83"><span style="background-color:#FFFFFF">1: Ladder </span></span>
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">2: Yib-pD </span></span>
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">3: BAPT-2 </span></span>
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">4: TAX10-1 </span></span>
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">5: TAX10-2</span></span><span style="color:#5C7C83"><span style="background-color:#FFFFFF"> </span></span>
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">6: DBAT-1</span></span>
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">7: DBAT-2</span></span>
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">8: TycA-2</span></span>
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part683" data-index="249" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part684" data-index="250" style="margin-left:0;"><span style="color:#D10E0E">Only TBT-2 showed the expected band size</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part685" data-index="251" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part686" data-index="252" style="margin-left:0;">Sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part687" data-index="253" style="margin-left:0;">Total of 16.5 uL of DNA/dilution in each sample tube</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part688-item" data-formattable="true" data-index="254" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:678px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-06-05 at 2.11.57 PM.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="6JoK1xqSHh9sK7jOH2PFW06ghHGNO7N8CK1Ehjv7-Screen_Shot_2016-06-05_at_2.11.57_PM.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part688" data-index="254">
 +
</div></div><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part690" data-index="255" style="margin-left:0;"><span style="color:#DE5555">5/28: Only TBT-2 and PAM-2 showed positive on the sequencing</span></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 5/31/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part691" data-index="256" style="margin-left:0;">Colony PCR of 5/23 Transformations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part692" data-index="257" style="margin-left:0;">Goal: To use probability to find colonies with inserts for those inserts that came back with an empty vector from sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part693" data-index="258" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part694" data-index="259" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part695" data-index="260" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part696-item" data-formattable="true" data-index="261" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="261" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part696-table" data-formattable="true" data-index="261" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part696-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part696-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part696-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part696-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part696-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part696-1-2" data-namespace="table-ns5179"><span>100</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part696-2-0" data-namespace="table-ns5179"><span>MM</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part696-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part696-2-2" data-namespace="table-ns5179"><span>1250</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part696-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part696-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part696-3-2" data-namespace="table-ns5179"><span>25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part696-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part696-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part696-4-2" data-namespace="table-ns5179"><span>25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part696-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part696-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part696-5-2" data-namespace="table-ns5179"><span>1200</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table5</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part696" data-index="261" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part697" data-index="262" style="margin-left:0;"><span style="color:#000000">Ran:</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part698" data-index="263" style="margin-left:35px;"><span style="color:#000000">PAM Colony and Miniprep</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part699" data-index="264" style="margin-left:35px;"><span style="color:#000000">TBT Miniprep</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part700" data-index="265" style="margin-left:35px;"><span style="color:#000000">BAPT 20 colonies</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part701" data-index="266" style="margin-left:35px;"><span style="color:#000000">TAX10 20 colonies</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part702" data-index="267" style="margin-left:35px;"><span style="color:#000000">DBAT 20 colonies</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part703" data-index="268" style="margin-left:35px;"><span style="color:#000000">TAT 14 colonies</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part704" data-index="269" style="margin-left:35px;"><span style="color:#000000">BadA 14 colonies</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part705" data-index="270" style="margin-left:35px;"><span style="color:#000000">pSMART 1 sample</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part706" data-index="271" style="margin-left:35px;"><span style="color:#000000">GFP 1 sample</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part707" data-index="272" style="margin-left:35px;"><span style="color:#000000">ProD 2 colonies</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part708" data-index="273" style="margin-left:0;">Used an elongation of 3.5 min</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part709" data-index="274" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part710" data-index="275" style="margin-left:0;">For Gel:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part711" data-index="276" style="margin-left:0;">Used a 100 well gell mold</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part712" data-index="277" style="margin-left:0;">Used 450 mL of agarose; 45 uL of dye</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part713" data-index="278" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part714" data-index="279" style="margin-left:35px;">Gels</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part715" data-index="280" style="margin-left:0;">Ran Gels: </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part716" data-index="281" style="margin-left:0;">Used 10 uL of DNA from PCR; 2 uL of dye</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part717" data-index="282" style="margin-left:0;">Ran for 35 min at 160 V</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part718" data-index="283" style="margin-left:0;">During loading, the pipette tips continuously retained some of sample or fell off</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part719" data-index="284" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part720" data-index="285" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part721-item" data-formattable="true" data-index="286" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:696px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">5:31:16 Big Bertha Row 1.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="9NWO0YwzG8wGfy3zCzgrZuTcvnjuBQE6YBJTic55-53116_Big_Bertha_Row_1.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part721" data-index="286"><span style="color:#5C7C83"><span style="background-color:#FFFFFF">1: Pro D
 +
2: Pro D
 +
3: PAM Colony
 +
4: PAM Mini prep
 +
5: TBT Mini prep
 +
6: GFP
 +
7: PSM
 +
8: Ladder
 +
9-29: BAPT 1-20</span></span>
 +
30: Ladder
 +
31-50: TAX10 1-20
 +
</div></div><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part723-item" data-formattable="true" data-index="287" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:696px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">5/31/2016 Big Bertha Row 2.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="0IAsst2TPpOxmkGtNZXVPh5CPFN3HgrcqxoTPr5y-5-31-2016_BigBertha2.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part723" data-index="287">1-14: TAT 1-14
 +
15: Ladder
 +
16-35: DBAT 1-20
 +
36: Ladder
 +
37-50: BadA 1-14
 +
</div></div><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part725" data-index="288" style="margin-left:0;"><span style="color:#DE5555">Only the TBT miniprep, PAM miniprep, TAT colony 9, and TAT colony 10 showed the expected band sizes.</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part726" data-index="289" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part727" data-index="290" style="margin-left:0;">Made dilution of sequence confirmed PAM and TBT</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part728" data-index="291" style="margin-left:0;">Diluted samples to 50 ng/uL </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part729" data-index="292" style="margin-left:35px;"><span style="color:#000000">For TBT Dilution, used 5.7 uL of DNA with 14.3 uL of pico H2O</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part730" data-index="293" style="margin-left:35px;"><span style="color:#000000">For PAM Dilution, used 3.3 uL of DNA with 16.7 uL of pico H2O</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part731" data-index="294" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part732" data-index="295" style="margin-left:0;">Inoculation of ProD Cultures </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part733" data-index="296" style="margin-left:0;">Picked 2 colonies from proD plate (proD-1 and proD-2)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part734" data-index="297" style="margin-left:0;">Inoculated in 10 mL of LB+KAN</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part735" data-index="298" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part736" data-index="299" style="margin-left:0;">Also Inoculated TAT 9, TAT 10, DBAT 16, DBAT 18, and DBAT 20.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part737" data-index="300" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part738" data-index="301" style="margin-left:0;">Transformations of TBT and TycA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part739" data-index="302" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part740" data-index="303" style="margin-left:35px;">Electroporation Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part741" data-index="304" style="margin-left:0;">TBT was transformed into two cultures</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part742" data-index="305" style="margin-left:35px;">TBT 1 - 1710 V 3.9 ms</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part743" data-index="306" style="margin-left:35px;">TBT 2 - 1710 V 4.0 ms</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part744" data-index="307" style="margin-left:0;">TycA was transformed into one culture</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part745" data-index="308" style="margin-left:35px;">TycA - 1580 V 654 ms</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part746" data-index="309" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part747" data-index="310" style="margin-left:0;">Recovered for 3 hours</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part748" data-index="311" style="margin-left:0;">3 uL of DNA and 20 uL of Comp Cells was used</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part749" data-index="312" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/1/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part750" data-index="313" style="margin-left:0;">Freezer Stocks of 5/31 Inoculations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part751" data-index="314" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part752" data-index="315" style="margin-left:35px;">Freezer Stock Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part753" data-index="316" style="margin-left:0;">Used 500 uL of glycerol and culture instead of 750 uL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part754" data-index="317" style="margin-left:0;">Created stocks of </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part755" data-index="318" style="margin-left:35px;">TAT 9</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part756" data-index="319" style="margin-left:35px;">TAT 10</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part757" data-index="320" style="margin-left:35px;">DBAT 16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part758" data-index="321" style="margin-left:35px;">DBAT 18</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part759" data-index="322" style="margin-left:35px;">DBAT 20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part760" data-index="323" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part761" data-index="324" style="margin-left:0;">Double Colony PCR</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part762" data-index="325" style="margin-left:0;"><span style="color:#000000">Mike’s method of smearing things on the side of the PCR tube before pipetting into the tube was used for all big colonies. The smaller colonies were dipped into the master mix.</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part763" data-index="326" style="margin-left:35px;"><span style="color:#000000">200uL pipette tips were used to pick and smear the colonies.</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part764" data-index="327" style="margin-left:35px;"><span style="color:#000000">Experimental setup:</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part765" data-index="328" style="margin-left:70px;"><span style="color:#000000">8 colonies from: TAX10, BAPT, BadA</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"><span>■</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part766" data-index="329" style="margin-left:105px;"><span style="color:#000000">2 of each colony, half tested on PCR machine in Teer basement lab and half in Lynch lab. Results will be compared later.</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part767" data-index="330" style="margin-left:70px;"><span style="color:#000000">4 controls: PAM-2 Colony, PAM-2 Miniprep, GFP Miniprep, pSmart Miniprep in both lab tests</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part768" data-index="331" style="margin-left:70px;"><span style="color:#000000">72 total reactions, made 74 Master mix according to the following:</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"><span>■</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part769" data-index="332" style="margin-left:105px;"><span style="color:#000000">1x // 74x</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"><span>■</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part770" data-index="333" style="margin-left:105px;"><span style="color:#000000">12.5 uL Econo // 925 uL</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"><span>■</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part771" data-index="334" style="margin-left:105px;"><span style="color:#000000">.25 uL SL1 // 18.5 uL</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"><span>■</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part772" data-index="335" style="margin-left:105px;"><span style="color:#000000">.25 uL SR2 // 18.5 uL</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"><span>■</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part773" data-index="336" style="margin-left:105px;"><span style="color:#000000">12 uL H2O // 888 uL</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"><span>■</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part774" data-index="337" style="margin-left:105px;"><span style="color:#000000">25 uL total // 1850 uL</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"><span>■</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part775" data-index="338" style="margin-left:105px;"><span style="color:#000000">NOTE: WE WERE 7 REACTIONS SHORT. PIPETTES NEED CALIBRATION</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part776" data-index="339" style="margin-left:70px;"><span style="color:#000000">Protocol used in Teer basement:</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"><span>■</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part777" data-index="340" style="margin-left:105px;"><span style="color:#000000">Start: 98C for 10 min.</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"><span>■</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part778" data-index="341" style="margin-left:105px;"><span style="color:#000000">Cycle (x40)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part779" data-index="342" style="margin-left:140px;"><span style="color:#000000">Melt 98C for 45s</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part780" data-index="343" style="margin-left:140px;"><span style="color:#000000">Anneal 50C for 45s</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part781" data-index="344" style="margin-left:140px;"><span style="color:#000000">Extend 72C for 3m 30s</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part782" data-index="345" style="margin-left:175px;"><span style="color:#000000">Time = (1 min / kbase)*length(longest amplificant)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:175px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part783" data-index="346" style="margin-left:175px;"><span style="color:#000000">Time = 1min/kbase * length(longest amplificant)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"><span>■</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part784" data-index="347" style="margin-left:105px;"><span style="color:#000000">4C for inf</span></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part785-item" data-formattable="true" data-index="348" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="note-protocol mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" href="/pjp11/f/zu5j4fT2-protocols/prt-GW3teHrd-colony-pcr/edit" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><svg class="svgIcon note-icon u-small-spacing-right pull-left"><use xlink:href=""></use></svg><div class="note-itemName">Colony PCR</div></h5></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part785" data-index="348">
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part786" data-index="349" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part787" data-index="350" style="margin-left:0;">Ran Gel on PCR in both Teer and in Lynch Labs</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part788-item" data-formattable="true" data-index="351" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:480px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">6-1-16_Angelica.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="3IvSxDXdHN7WZaVUUFtzG4OuGeWlQAv03WYKFw1w-6-1-16_Angelica.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part788" data-index="351">1: Ladder
 +
2: GFP
 +
3: PAM - Mini prep
 +
4: PAM Colony
 +
5: pSMART
 +
6-13: BadA A-H
 +
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part790-item" data-formattable="true" data-index="352" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:480px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">6-1-16_Brandon.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Bgmok0ZVS0XwcB1Bb5yUS9yiaO5MtWUwcLjaEaNk-6-1-16_Brandon.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part790" data-index="352">1:Ladder
 +
2-9: TAX10 A-H
 +
10-13: BadA <u>A-D</u>
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part792-item" data-formattable="true" data-index="353" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:477px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">6-1-16_Suzie.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="NYLf89199eRHSuenX73XI7luW6QqSyonzmT8Nxx6-6-1-16_Suzie.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part792" data-index="353">1: Ladder
 +
2-9: BAPT A-H
 +
10-13: BadA <u>E-H</u>
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part794-item" data-formattable="true" data-index="354" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:474px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">6-1-16_Gel1.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="EB9y8BfnqERrZSKlxHVY1kcyqAvnYIReQ8Senpom-6-1-16_Gel1.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part794" data-index="354">1: Ladder
 +
2-5: BAPT A-D
 +
6: Empty
 +
7: PAM Miniprep
 +
8: PAM Colony
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part796-item" data-formattable="true" data-index="355" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:473px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">6-1-16_Gel2.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="UWwgguB2DfZz6pkFQzdu1e6BQWG0vHyE73tla3Ev-6-1-16_Gel2.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part796" data-index="355">1: Ladder
 +
2-5: BAPT E-H
 +
6: pSMART
 +
7: GFP
 +
8: Empty
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part798-item" data-formattable="true" data-index="356" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:473px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">6-1-16_Gel3.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="EJ9dpcRJB4eDoXooG9VccKnV8BraFMnyurcfQ2sH-6-1-16_Gel3.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part798" data-index="356">1: Ladder
 +
2-3: TAX10 G-H
 +
4-11: BadA A-H
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part800-item" data-formattable="true" data-index="357" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:468px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">6-1-16_Gel4.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="E3VJhwzGKwTiVsiC0TLrJLwmxXYs3zJdtrhnNJM7-6-1-16_Gel4.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part800" data-index="357">1: Ladder
 +
2-9: BadA <u>A-H</u>
 +
10-15: TAX10 A-F
 +
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part802" data-index="358" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part803" data-index="359" style="margin-left:0;"><span style="color:#D10E0E">In the Teer Gels Positive Resutls included:</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part804" data-index="360" style="margin-left:35px;"><span style="color:#D10E0E">TAX10- (A,B,D,E,G,H)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part805" data-index="361" style="margin-left:35px;"><span style="color:#D10E0E">BAPT- (B, D, E, G, H)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part806" data-index="362" style="margin-left:35px;"><span style="color:#D10E0E">badA- (<u>A, B, C, D, F, G, H</u>)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part807" data-index="363" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part808" data-index="364" style="margin-left:0;">Inoculation of Positive Colonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part809" data-index="365" style="margin-left:0;">Colonies denoted in red in the above results were inoculated in 5 mL of LB + KAN and left in the shaker over night</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part810" data-index="366" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part811" data-index="367" style="margin-left:0;">Mini prep/Concentration Check of Successful Transformations from 5/31 (Nisa; Thomas)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part812" data-index="368" style="margin-left:0;">Protocols:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part813" data-index="369" style="margin-left:35px;">Miniprep Protocol Zyppy</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part814" data-index="370" style="margin-left:35px;">Testing Miniprep Concentrations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part815" data-index="371" style="margin-left:0;">Miniprep Edit: Used 50 uL elution</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part816" data-index="372" style="margin-left:0;"> </div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part817-item" data-formattable="true" data-index="373" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="373" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part817-table" data-formattable="true" data-index="373" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:665px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:645px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part817-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part817-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAT-9</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part817-0-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAT-10</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part817-0-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>DBAT-16</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part817-0-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>DBAT-18</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part817-0-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>DBAT-20</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part817-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>ng/uL (Concentration)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part817-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>121</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part817-1-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>80</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part817-1-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>117</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part817-1-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>72</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part817-1-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>55</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table6</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part817" data-index="373" style="width:665px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/2/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part818" data-index="374" style="margin-left:0;">Colony PCR on TycA Plate (Emma; Ben)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part819" data-index="375" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part820" data-index="376" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part821" data-index="377" style="margin-left:0;">Duplicate NOT run in Lynch Lab</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part822" data-index="378" style="margin-left:0;">Used an elongation of 4.5 min</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part823" data-index="379" style="margin-left:0;">Smeared 10 colonies using Mike's method</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part824" data-index="380" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part825" data-index="381" style="margin-left:0;">Ran a Gel </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part826-item" data-formattable="true" data-index="382" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:415px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">6-2-2016 Angelica (TycA).jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="NjzuuKUDl3YT44qbR02Y3AbrG4GA8AOJ7yHONGsn-6-2-2016_Angelica_TycA.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part826" data-index="382">1: Ladder
 +
2: GFP
 +
3: PAM Mini prep
 +
4: PAM Colony
 +
5: pSMART
 +
6-9: TycA 1-4
 +
10: Spillage from TycA 4
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part828" data-index="383" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part829-item" data-formattable="true" data-index="384" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:418px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">6-2-2016 Brandon (TycA).jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="g62DJHQJcuBrb9k2jGJGC4m54w9RspzMVJULdfXC-6-2-2016_Brandon_TycA.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part829" data-index="384">1: Ladder
 +
2: GFP
 +
3: PAM Colony
 +
4-5: TycA 9-10
 +
6: Empty
 +
7-10: TycA 5-8
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part831" data-index="385" style="margin-left:0;">Due to Gel results, TycA Samples 1-6, 9, and 10 were chosen for inoculation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part832" data-index="386" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part833" data-index="387" style="margin-left:0;">Inoculation of Colonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part834" data-index="388" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">TycA Samples 1-6, 9, and 10 were inoculated in 5 mL of LB+KAN</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part835" data-index="389" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part836" data-index="390" style="margin-left:0;">Freezer Stocks/Miniprep/Concentration Check/Sequencing of Successful 6/1 Transformations (Parth, Nisa)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part837" data-index="391" style="margin-left:0;">Samples:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part838" data-index="392" style="margin-left:35px;">TAX10- (A,B,D,E,G,H)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part839" data-index="393" style="margin-left:35px;">BAPT- (B, D, E, G, H)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part840" data-index="394" style="margin-left:35px;">badA- (A, B, C, D, F, G, H)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part841" data-index="395" style="margin-left:0;">Protocols:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part842" data-index="396" style="margin-left:35px;">Freezer Stocks Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part843" data-index="397" style="margin-left:35px;">Miniprep Protocol Zyppy</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part844" data-index="398" style="margin-left:35px;">Testing Miniprep Concentrations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part845" data-index="399" style="margin-left:35px;">Preparation for Sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part846" data-index="400" style="margin-left:0;">Miniprep Edit: Used a 50 uL elution</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part847" data-index="401" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part848-item" data-formattable="true" data-index="402" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="402" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part848-table" data-formattable="true" data-index="402" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:606px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:586px;"><colgroup><col style="width:45px;"></col><col style="width:92px;"></col><col style="width:57px;"></col><col style="width:58px;"></col><col style="width:57px;"></col><col style="width:57px;"></col><col style="width:59px;"></col><col style="width:61px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">H</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample:</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10-A</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-0-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10-B</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-0-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10-D</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-0-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10-E</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-0-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10-G</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-0-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10-H</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-0-7" data-namespace="table-ns5179" style="color:#DE5555;"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Concentration (ng/uL)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>69</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-1-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>203</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-1-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>210</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-1-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>190</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-1-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>132</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-1-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>94</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-1-7" data-namespace="table-ns5179" style="color:#DE5555;"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-2-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample:</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-2-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT-B</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-2-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT-D</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-2-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT-E</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-2-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT-G</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-2-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT-H</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-2-6" data-namespace="table-ns5179" style="color:#DE5555;"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-2-7" data-namespace="table-ns5179" style="color:#DE5555;"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-3-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Concentration (ng/uL)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-3-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>61</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-3-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>51</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-3-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>146</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-3-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>142</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-3-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>180</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-3-6" data-namespace="table-ns5179" style="color:#DE5555;"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-3-7" data-namespace="table-ns5179" style="color:#DE5555;"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-4-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample:</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-4-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>badA-A</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-4-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>badA-B</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-4-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>badA-C</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-4-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>badA-D</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-4-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>badA-F</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-4-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>badA-G</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-4-7" data-namespace="table-ns5179" style="color:#DE5555;"><span>badA-H</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part848-5-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Concentration (ng/uL)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-5-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>52</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-5-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>209</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-5-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>60</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-5-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>100</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-5-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>165</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-5-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>149</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part848-5-7" data-namespace="table-ns5179" style="color:#DE5555;"><span>130</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table9</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part848" data-index="402" style="width:606px;margin-left:0;">Results of Testing DNA Concentration
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/3/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part849" data-index="403" style="margin-left:0;">Freezer Stocks/Miniprep/Concentration Check of TycA </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part850" data-index="404" style="margin-left:0;">Labels were pGEM40-48 for samples TycA 1, 2, 3, 4, 5, 6, 8, 9, 10</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part851" data-index="405" style="margin-left:0;">Protocols:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part852" data-index="406" style="margin-left:35px;">Freezer Stocks Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part853" data-index="407" style="margin-left:35px;">Miniprep Protocol Zyppy</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part854" data-index="408" style="margin-left:35px;">Testing Miniprep Concentrations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part855" data-index="409" style="margin-left:0;">Miniprep Edit: First step is 3500 rmp for 8 min</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part856" data-index="410" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part857-item" data-formattable="true" data-index="411" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="411" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part857-table" data-formattable="true" data-index="411" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:329px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:309px;"><colgroup><col style="width:45px;"></col><col style="width:92px;"></col><col style="width:57px;"></col><col style="width:58px;"></col><col style="width:57px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample:</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>tycA 1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-0-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA 2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-0-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA 3</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Concentration (ng/uL)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part857-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>83</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part857-1-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part857-1-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>42</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-2-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample:</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-2-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA 4</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-2-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA 5</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-2-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA 6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-3-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Concentration (ng/uL)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part857-3-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>75</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part857-3-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>36</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part857-3-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>53</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-4-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample:</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-4-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA 8</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-4-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA 9</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-4-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA 10</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part857-5-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Concentration (ng/uL)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part857-5-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>51</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part857-5-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>51</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part857-5-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>71</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table11</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part857" data-index="411" style="width:329px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part858" data-index="412" style="margin-left:0;">Transformations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part859" data-index="413" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part860" data-index="414" style="margin-left:35px;">Electroporation Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part861" data-index="415" style="margin-left:0;">DBAT, BAPT, TAX10, and TycA were transformed into competent E Cloni Cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part862" data-index="416" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part863" data-index="417" style="margin-left:0;">Recovered in Shaker for 2 hours. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part864" data-index="418" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part865-item" data-formattable="true" data-index="419" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="419" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part865-table" data-formattable="true" data-index="419" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part865-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part865-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>Volts</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part865-0-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>milliseconds</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part865-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part865-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1520</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part865-1-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>654</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part865-2-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part865-2-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1540</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part865-2-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>654</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part865-3-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>DBAT 1 </span></td><td class="cell is-value is-alignRight" data-cid="table-note-part865-3-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1570</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part865-3-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.9</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part865-4-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>DBAT 2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part865-4-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1570</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part865-4-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>654</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part865-5-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA 1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part865-5-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1610</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part865-5-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>1.3</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part865-6-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA 2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part865-6-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1630</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part865-6-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>1.3</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table10</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part865" data-index="419" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part866" data-index="420" style="margin-left:0;">Transformations were then plated:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part867" data-index="421" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part868-item" data-formattable="true" data-index="422" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="422" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part868-table" data-formattable="true" data-index="422" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-0-0" data-namespace="table-ns5179"><span>Sample</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-0-1" data-namespace="table-ns5179"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-0-2" data-namespace="table-ns5179"><span>100 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-1-0" data-namespace="table-ns5179"><span>BAPT</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-1-1" data-namespace="table-ns5179"><span>A</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-1-2" data-namespace="table-ns5179"><span>B</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-2-0" data-namespace="table-ns5179"><span>TAX10</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-2-1" data-namespace="table-ns5179"><span>A</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-2-2" data-namespace="table-ns5179"><span>B</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-3-0" data-namespace="table-ns5179"><span>DBAT 1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-3-1" data-namespace="table-ns5179"><span>A</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-3-2" data-namespace="table-ns5179"><span>B</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-4-0" data-namespace="table-ns5179"><span>DBAT 2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-4-1" data-namespace="table-ns5179"><span>A</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part868-4-2" data-namespace="table-ns5179"><span>B</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table12</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part868" data-index="422" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part869" data-index="423" style="margin-left:0;">TycA 1 and TycA 2 were both plated with 200 uL </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part870" data-index="424" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part871" data-index="425" style="margin-left:0;">Inoculated TycA </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part872" data-index="426" style="margin-left:0;">(1-8) small colonies and placed in shaker</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part873" data-index="427" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part874" data-index="428" style="margin-left:0;">Sent for Sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part875" data-index="429" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part876" data-index="430" style="margin-left:35px;">Preparation for Sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part877" data-index="431" style="margin-left:0;">These samples were sent for sequencing:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part878" data-index="432" style="margin-left:35px;">TycA 1-6, 8-10</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part879" data-index="433" style="margin-left:35px;">BadA-B </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part880" data-index="434" style="margin-left:0;">Did not dilute,</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part881" data-index="435" style="margin-left:0;">Sent 10 uL of each sample </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part882" data-index="436" style="margin-left:0;">Only sent in 1 primer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part883" data-index="437" style="margin-left:35px;">TycA sent in with SL1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part884" data-index="438" style="margin-left:35px;">BadA-B sent in with SR2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part885" data-index="439" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part886" data-index="440" style="margin-left:0;">Diluted Samples</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part887" data-index="441" style="margin-left:0;">Diluted samples of BadA-B and TAT-9 to 50 ng/uL </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part888" data-index="442" style="margin-left:35px;">BadA =&gt; 1.2 uL DNA; 3.8 uL pH2O</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part889" data-index="443" style="margin-left:35px;">TAT =&gt; 2.1 uL DNA; 2.9 uL pH2O</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part890" data-index="444" style="margin-left:0;">Colony PCR</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part891" data-index="445" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part892" data-index="446" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part893" data-index="447" style="margin-left:0;">Made a double PCR; ran one set on the old PCR machine; ran the new set on the new PCR machine</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part894" data-index="448" style="margin-left:0;">Samples run were: </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part895" data-index="449" style="margin-left:35px;">pSMART</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part896" data-index="450" style="margin-left:35px;">GFP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part897" data-index="451" style="margin-left:35px;">ProD Colony</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part898" data-index="452" style="margin-left:35px;">ProD Colony</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part899" data-index="453" style="margin-left:35px;">PAM-2 Colony</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part900" data-index="454" style="margin-left:35px;">PAM-2 Miniprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part901" data-index="455" style="margin-left:35px;">TBT Miniprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part902" data-index="456" style="margin-left:35px;">BadA-B Miniprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part903" data-index="457" style="margin-left:35px;">TAT-9 Miniprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part904" data-index="458" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part905-item" data-formattable="true" data-index="459" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="459" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part905-table" data-formattable="true" data-index="459" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part905-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part905-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part905-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part905-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part905-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part905-1-2" data-namespace="table-ns5179"><span>24</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part905-2-0" data-namespace="table-ns5179"><span>MM</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part905-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part905-2-2" data-namespace="table-ns5179"><span>300</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part905-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part905-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part905-3-2" data-namespace="table-ns5179"><span>6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part905-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part905-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part905-4-2" data-namespace="table-ns5179"><span>6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part905-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part905-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part905-5-2" data-namespace="table-ns5179"><span>288</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table13</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part905" data-index="459" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part906" data-index="460" style="margin-left:0;">Added a new protocol to the new PCR machine</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part907" data-index="461" style="margin-left:35px;">Saved in a new folder "IGEM 2016" as "IGEM COL V1"</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part908" data-index="462" style="margin-left:70px;">Ran for 30s at 50, 72, and 98 with an elongation of 4.5 min</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part909" data-index="463" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part910" data-index="464" style="margin-left:0;">Notes:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part911" data-index="465" style="margin-left:0;">BadA is Sequence Confirmed</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part912" data-index="466" style="margin-left:0;">Tax10 and BAPT were blanks</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 6/4/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part913" data-index="467" style="margin-left:0;">Freezer Stocks of TycA and addition to Database</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part914" data-index="468" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part915" data-index="469" style="margin-left:35px;">Freezer Stock Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part916" data-index="470" style="margin-left:0;">Labels were pGEM049-056 for samples TycA 1, 2, 3, 4, 5, 6, 7, 8</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part917" data-index="471" style="margin-left:0;">Mini Prep Inoculations for TycA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part918" data-index="472" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part919" data-index="473" style="margin-left:35px;">Miniprep Protocol NEB</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part920" data-index="474" style="margin-left:0;">The following inoculations were mini prepped:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part921" data-index="475" style="margin-left:35px;">TycA 1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part922" data-index="476" style="margin-left:35px;">TycA 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part923" data-index="477" style="margin-left:35px;">TycA 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part924" data-index="478" style="margin-left:35px;">TycA 4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part925" data-index="479" style="margin-left:35px;">TycA 5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part926" data-index="480" style="margin-left:35px;">TycA 7</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part927" data-index="481" style="margin-left:35px;">TycA 8</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part928" data-index="482" style="margin-left:35px;">TycA 9</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part929" data-index="483" style="margin-left:0;">Used a 50 uL elution. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part930" data-index="484" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part931" data-index="485" style="margin-left:0;">Left minipreps for concentration testing on monday.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part932" data-index="486" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part933" data-index="487" style="margin-left:0;"><span style="color:#DE5555">Concentrations (from 6/6 for these minipreps):</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part934" data-index="488" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part935" data-index="489" style="margin-left:35px;">Testing Miniprep Concentrations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part936" data-index="490" style="margin-left:0;">Tested Concentrations using nanodrop</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part937-item" data-formattable="true" data-index="491" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="491" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part937-table" data-formattable="true" data-index="491" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:557px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:537px;"><colgroup><col style="width:45px;"></col><col style="width:106px;"></col><col style="width:53px;"></col><col style="width:57px;"></col><col style="width:54px;"></col><col style="width:59px;"></col><col style="width:50px;"></col><col style="width:57px;"></col><col style="width:56px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">H</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA-2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-0-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA-3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-0-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA-4</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-0-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA-5</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-0-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA-7</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-0-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA-8</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-0-7" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA-9</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Round 1 (ng/uL)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>33</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-1-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>10</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-1-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>22</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-1-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>6</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-1-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>3</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-1-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-1-7" data-namespace="table-ns5179" style="color:#DE5555;"><span>5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-2-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Round 2 (ng/uL)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-2-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>13</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-2-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>43</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-2-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>52</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-2-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>3</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-2-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>19</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-2-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>4</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-2-7" data-namespace="table-ns5179" style="color:#DE5555;"><span>22</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-3-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Concentration used:</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-3-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>33</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-3-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>43</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-3-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>52</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-3-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>N/A </span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-3-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>19</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part937-3-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>N/A </span></td><td class="cell is-value is-alignRight" data-cid="table-note-part937-3-7" data-namespace="table-ns5179" style="color:#DE5555;"><span>22</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table17</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part937" data-index="491" style="width:557px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part938" data-index="492" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part939" data-index="493" style="margin-left:0;">Ran Gel on 6/3 PCR</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part940" data-index="494" style="margin-left:0;">The two PCR on 6/2 were run on two PCR machines. Samples tested in Angelica were from the old PCR machine and samples tested on Brandon were from the new PCR machine.</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part941-item" data-formattable="true" data-index="495" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06/03/2016 Angelica JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="fYKcRSdC1TjrZMxNEWquXBmKlDFLp9SHglPqIoX7-IMG_1709.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part941" data-index="495">30s denature, 30s anneal, 3:30 elongation, 35 cycles, oldmachine
 +
1: Ladder
 +
2: GFP
 +
3: pSMART
 +
4: PAM Miniprep
 +
5: TBT Miniprep
 +
6: TAT Miniprep
 +
7: BAdA Miniprep
 +
8: PAM Colony
 +
9: ProD 1
 +
10: ProD2
 +
11-13: Empty
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part943-item" data-formattable="true" data-index="496" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06/03/2016 Brandon.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="iV8rZmO6SjCquzx6MY6CRjdf3iPY5rnLWXisFhds-IMG_1710.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part943" data-index="496"><span style="color:#5C7C83"><span style="background-color:#FFFFFF">30s denature, 30s anneal, 3:30 elongation, 35 cycles, new machine</span></span>
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">1: Ladder
 +
2: GFP
 +
3: pSMART
 +
4: PAM Miniprep
 +
5: TBT Miniprep
 +
6: TAT Miniprep
 +
7: BAdA Miniprep
 +
8: PAM Colony
 +
9: ProD 1
 +
10: ProD2
 +
11-13: Empty</span></span>
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part945" data-index="497" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part946" data-index="498" style="margin-left:0;">Plated Gibson Transformations (Parth)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part947" data-index="499" style="margin-left:0;">DBAT, TAX10, and BAPT were all plated on to Kan plates with the following uL amounts.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part948" data-index="500" style="margin-left:35px;">10 uL, 50 uL, 100 uL of transformation per enzyme</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part949" data-index="501" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 6/5/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part950" data-index="502" style="margin-left:0;">Colony PCR of Controls </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part951" data-index="503" style="margin-left:0;">Goal: Testing if the 45 second annealing and denature steps affected controls</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part952" data-index="504" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part953" data-index="505" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part954" data-index="506" style="margin-left:0;"> PCR of controls: </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part955" data-index="507" style="margin-left:35px;">Standard PCR mix used</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part956" data-index="508" style="margin-left:35px;">Miniprepped DNA diluted to 50ng/ul concentration</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part957" data-index="509" style="margin-left:0;">Samples: GFP, Psmart, Pam Mini, TBT mini, TAT mini, BADA Mini, PAM colony, ProD 1, Prod D2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part958" data-index="510" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part959-item" data-formattable="true" data-index="511" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="511" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part959-table" data-formattable="true" data-index="511" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part959-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part959-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part959-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part959-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part959-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part959-1-2" data-namespace="table-ns5179"><span>24</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part959-2-0" data-namespace="table-ns5179"><span>MM</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part959-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part959-2-2" data-namespace="table-ns5179"><span>300</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part959-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part959-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part959-3-2" data-namespace="table-ns5179"><span>6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part959-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part959-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part959-4-2" data-namespace="table-ns5179"><span>6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part959-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part959-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part959-5-2" data-namespace="table-ns5179"><span>288</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part959-6-0" data-namespace="table-ns5179"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part959-6-1" data-namespace="table-ns5179"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part959-6-2" data-namespace="table-ns5179"><span>600</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table16</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part959" data-index="511" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part960" data-index="512" style="margin-left:0;">Ran Gel</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part961-item" data-formattable="true" data-index="513" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">image (4).jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="fxdwtQ1HSUJkQwRvjuZWKgwma6c58skdgppyreLr-image_4.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part961" data-index="513">45s denature, 45s anneal 3:30 elongation 35 cycles, Old machine
 +
1:Ladder
 +
2:GFP
 +
3:pSMART
 +
4:PAM miniprep
 +
5:TBT miniprep
 +
6:TAT miniprep
 +
7:badA miniprep
 +
8:proD colony
 +
9:proD colony
 +
 +
</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/6/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part963" data-index="514" style="margin-left:0;">Colony PCR (Ben; Nisa)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part964" data-index="515" style="margin-left:0;">Goal: Try different times and protocols to see where our controls stopped working</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part965" data-index="516" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part966" data-index="517" style="margin-left:0;">Notes:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part967" data-index="518" style="margin-left:0;">-Running 4:30 elongation time on old PCR (Mike) and new PCRA (Charlie). 3:30 on new PCRB (NEB)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part968" data-index="519" style="margin-left:0;">- Ran with 40x cycles</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part969" data-index="520" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part970" data-index="521" style="margin-left:0;">Samples used:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part971" data-index="522" style="margin-left:0;">- PSM, GFP, PAM C, PAM M , BadA M, TBT M, TAT M, ProD C, ProD C.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part972" data-index="523" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part973" data-index="524" style="margin-left:0;">(Time in: About 10:20a)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part974" data-index="525" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part975-item" data-formattable="true" data-index="526" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="526" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part975-table" data-formattable="true" data-index="526" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:562px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:542px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:97px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part975-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part975-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part975-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part975-0-3" data-namespace="table-ns5179"><span>Using Old MM (Used in the first 6 that went into Mike)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part975-0-4" data-namespace="table-ns5179"><span>New MM to make</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part975-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part975-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-1-2" data-namespace="table-ns5179"><span>30</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-1-3" data-namespace="table-ns5179"><span>6</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-1-4" data-namespace="table-ns5179"><span>24</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part975-2-0" data-namespace="table-ns5179"><span>MM</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-2-2" data-namespace="table-ns5179"><span>375</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-2-3" data-namespace="table-ns5179"><span>75</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-2-4" data-namespace="table-ns5179"><span>300</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part975-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-3-2" data-namespace="table-ns5179"><span>7.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-3-3" data-namespace="table-ns5179"><span>1.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-3-4" data-namespace="table-ns5179"><span>6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part975-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-4-2" data-namespace="table-ns5179"><span>7.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-4-3" data-namespace="table-ns5179"><span>1.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-4-4" data-namespace="table-ns5179"><span>6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part975-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-5-2" data-namespace="table-ns5179"><span>360</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-5-3" data-namespace="table-ns5179"><span>72</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-5-4" data-namespace="table-ns5179"><span>288</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part975-6-0" data-namespace="table-ns5179"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-6-1" data-namespace="table-ns5179"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-6-2" data-namespace="table-ns5179"><span>750</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-6-3" data-namespace="table-ns5179"><span>150</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part975-6-4" data-namespace="table-ns5179"><span>600</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table14</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part975" data-index="526" style="width:562px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part976" data-index="527" style="margin-left:0;">Left Gels for tomorrow</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part977" data-index="528" style="margin-left:0;">Miniprep of DBAT, TAX10, and BAPT (Jay &gt; Ben, Parth)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part978" data-index="529" style="margin-left:0;">Goal: Miniprep....</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part979" data-index="530" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part980" data-index="531" style="margin-left:35px;">Miniprep Protocol NEB</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part981" data-index="532" style="margin-left:0;">50 uL elution of dH2O</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part982" data-index="533" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part983" data-index="534" style="margin-left:0;">Inoculation samples used:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part984" data-index="535" style="margin-left:35px;">TAX10 (1-6)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part985" data-index="536" style="margin-left:35px;">DBAT (1-6)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part986" data-index="537" style="margin-left:35px;">BAPT (1-6)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part987" data-index="538" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part988" data-index="539" style="margin-left:0;"><span style="color:#DE5555">Concentrations:</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part989" data-index="540" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part990" data-index="541" style="margin-left:35px;">Testing Miniprep Concentrations</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part991-item" data-formattable="true" data-index="542" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="542" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part991-table" data-formattable="true" data-index="542" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:374px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:354px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:38px;"></col><col style="width:26px;"></col><col style="width:36px;"></col><col style="width:42px;"></col><col style="width:35px;"></col><col style="width:32px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part991-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10 Sample</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-0-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-0-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>3</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-0-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>4</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-0-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-0-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part991-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Concentration (ng/uL)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>127</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-1-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>119</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-1-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>-12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-1-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>40</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-1-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>98</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-1-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>84</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part991-2-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>DBAT Sample</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-2-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-2-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-2-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>3</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-2-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>4</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-2-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-2-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part991-3-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Concentration (ng/uL)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-3-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>-2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-3-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>59</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-3-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>122</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-3-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>13</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-3-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>116</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-3-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>63</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part991-4-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT Sample</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-4-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-4-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-4-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>3</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-4-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>4</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-4-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-4-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part991-5-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Concentration (ng/uL)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-5-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>24</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-5-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>41</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-5-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>23</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-5-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>14</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-5-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>73</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part991-5-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>100</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table18</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part991" data-index="542" style="width:374px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part992" data-index="543" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part993" data-index="544" style="margin-left:0;">Transformation of Sequence Confirmed Mini prep into 286 Cells(Thomas; Emma)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part994" data-index="545" style="margin-left:0;">Goal: That our miniprepped sequence confirmed cells can be transformed into the more robust 286 cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part995" data-index="546" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part996" data-index="547" style="margin-left:35px;">Electroporation Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part997" data-index="548" style="margin-left:0;">Details:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part998" data-index="549" style="margin-left:35px;">Transformed: sequence confirmed minipreps of PAM-2, TBT-2, TAT-9, BadA-B</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part999" data-index="550" style="margin-left:35px;">Used 500ul tubes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1000" data-index="551" style="margin-left:35px;"> because of a shortage of 1.5ml tubes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1001" data-index="552" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1002" data-index="553" style="margin-left:0;">left incubator for 2.5 hours</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1003" data-index="554" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1004" data-index="555" style="margin-left:0;">Recorded volts during electroporation</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1005-item" data-formattable="true" data-index="556" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="556" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1005-table" data-formattable="true" data-index="556" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1005-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1005-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>Volts</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1005-0-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>Miliseconds</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1005-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAT -9 A</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1005-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>610</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1005-1-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>654</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1005-2-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAT-9 B</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1005-2-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1690</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1005-2-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>3.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1005-3-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>BadA B</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1005-3-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1680</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1005-3-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>3.8</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1005-4-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>PAM-2 </span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1005-4-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1680</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1005-4-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>3.4</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1005-5-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>TBT-2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1005-5-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1710</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1005-5-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>4</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table15</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1005" data-index="556" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1006" data-index="557" style="margin-left:0;">Sequencing (Parth, Thomas)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1007" data-index="558" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1008" data-index="559" style="margin-left:35px;">Preparation for Sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1009" data-index="560" style="margin-left:0;">Sent the following samples for sequencing along with SL1 primer:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1010" data-index="561" style="margin-left:35px;">TycA- (2,3,4,7,9)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1011" data-index="562" style="margin-left:35px;">TAX10- (1,2,4,5,6)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1012" data-index="563" style="margin-left:35px;">DBAT- (2,4,5,6)*</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1013" data-index="564" style="margin-left:70px;">Accidentally sent sample 4 instead of 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1014" data-index="565" style="margin-left:35px;">BAPT- (2,5,6)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1015" data-index="566" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/7/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1016" data-index="567" style="margin-left:0;">Ran Gels for PCR Colony on 6/6</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1017" data-index="568" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1018" data-index="569" style="margin-left:35px;">Gels</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1019" data-index="570" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1020-item" data-formattable="true" data-index="571" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Mike-4m30s_elong.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="djEF8hSKYdwVGQforKJwnfgeIKjZZndhSSfkwcb3-Mike-4m30s_elong.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1020" data-index="571">denature 45s, anneal 45s, elongation 4:30, 40 cycles, old machine
 +
1: Ladder
 +
2: pSMART
 +
3: GFP
 +
4: PAM Colony
 +
5: PAM Miniprep
 +
6: BadA Miniprep
 +
7: TBT Miniprep
 +
8:TAT Miniprep
 +
9: ProD Colony
 +
10: ProD Colony
 +
11-13: Empty
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1022" data-index="572" style="margin-left:0;">
 +
</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1023-item" data-formattable="true" data-index="573" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Charlie-4m30s_elong.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="0yUJq70ypcxmJyKvuEuL3SnTrsFp95vApMbKVxv8-Charlie-4m30s_elong.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1023" data-index="573"><span style="color:#5C7C83"><span style="background-color:#FFFFFF">denature 45s, anneal 45s, elongation 4:30, 40 cycles, new machine</span></span>
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">1: Ladder
 +
2: pSMART
 +
3: GFP
 +
4: PAM Colony
 +
5: PAM Miniprep
 +
6: BadA Miniprep
 +
7: TBT Miniprep
 +
8:TAT Miniprep
 +
9: ProD Colony
 +
10: ProD Colony
 +
11-13: Empty</span></span>
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1025" data-index="574" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1026-item" data-formattable="true" data-index="575" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">NEB-3m30s_elong.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Nfyn2Ly80lGvTCtJ2puTRxdSDW4aYQEaZnOtzt1T-NEB-3m30s_elong.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1026" data-index="575"><span style="color:#5C7C83"><span style="background-color:#FFFFFF">denature 45s, anneal 45s, elongation 3:30, 40 cycles, new machine</span></span>
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">1: Ladder
 +
2: pSMART
 +
3: GFP
 +
4: PAM Colony
 +
5: PAM Miniprep
 +
6: BadA Miniprep
 +
7: TBT Miniprep
 +
8:TAT Miniprep
 +
9: ProD Colony
 +
10: ProD Colony
 +
11-13: Empty</span></span>
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1028" data-index="576" style="margin-left:0;">Well that didn't work quite right... Time for the next experiment!</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1029" data-index="577" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1030" data-index="578" style="margin-left:0;">PCR again: Find working Protocol for long TycA inserts
 +
</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1031" data-index="579" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1032" data-index="580" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1033" data-index="581" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1034" data-index="582" style="margin-left:0;">GFP, Psm, PAM-M, PAM-C, TBT-M, TBT-C, TAT-M, TAT-C, BadA-M, BadA-C, ProD-1, ProD-2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1035" data-index="583" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1036" data-index="584" style="margin-left:0;">Note: Add in 1 uL of 20-50ng/uL DNA.</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1037-item" data-formattable="true" data-index="585" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="585" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1037-table" data-formattable="true" data-index="585" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1037-0-0" data-namespace="table-ns5179" style="color:#344043;"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1037-0-1" data-namespace="table-ns5179" style="color:#344043;"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1037-0-2" data-namespace="table-ns5179" style="color:#344043;"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1037-1-0" data-namespace="table-ns5179" style="color:#344043;"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1037-1-1" data-namespace="table-ns5179" style="color:#344043;"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1037-1-2" data-namespace="table-ns5179" style="color:#344043;"><span>38</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1037-2-0" data-namespace="table-ns5179" style="color:#344043;"><span>MM</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1037-2-1" data-namespace="table-ns5179" style="color:#344043;"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1037-2-2" data-namespace="table-ns5179" style="color:#344043;"><span>475</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1037-3-0" data-namespace="table-ns5179" style="color:#344043;"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1037-3-1" data-namespace="table-ns5179" style="color:#344043;"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1037-3-2" data-namespace="table-ns5179" style="color:#344043;"><span>9.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1037-4-0" data-namespace="table-ns5179" style="color:#344043;"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1037-4-1" data-namespace="table-ns5179" style="color:#344043;"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1037-4-2" data-namespace="table-ns5179" style="color:#344043;"><span>9.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1037-5-0" data-namespace="table-ns5179" style="color:#344043;"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1037-5-1" data-namespace="table-ns5179" style="color:#344043;"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1037-5-2" data-namespace="table-ns5179" style="color:#344043;"><span>456</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1037-6-0" data-namespace="table-ns5179" style="color:#344043;"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1037-6-1" data-namespace="table-ns5179" style="color:#344043;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1037-6-2" data-namespace="table-ns5179" style="color:#344043;"><span>950</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table7</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1037" data-index="585" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1038" data-index="586" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1039" data-index="587" style="margin-left:0;">Labels:</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1040-item" data-formattable="true" data-index="588" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="588" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1040-table" data-formattable="true" data-index="588" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:670px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:650px;"><colgroup><col style="width:45px;"></col><col style="width:50px;"></col><col style="width:57px;"></col><col style="width:52px;"></col><col style="width:44px;"></col><col style="width:43px;"></col><col style="width:62px;"></col><col style="width:53px;"></col><col style="width:54px;"></col><col style="width:51px;"></col><col style="width:44px;"></col><col style="width:50px;"></col><col style="width:45px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">H</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">I</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">J</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">K</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">L</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part1040-0-0" data-namespace="table-ns5179"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1040-0-1" data-namespace="table-ns5179"><span>2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1040-0-2" data-namespace="table-ns5179"><span>3</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1040-0-3" data-namespace="table-ns5179"><span>4</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1040-0-4" data-namespace="table-ns5179"><span>5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1040-0-5" data-namespace="table-ns5179"><span>6</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1040-0-6" data-namespace="table-ns5179"><span>7</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1040-0-7" data-namespace="table-ns5179"><span>8</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1040-0-8" data-namespace="table-ns5179"><span>9</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1040-0-9" data-namespace="table-ns5179"><span>10</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1040-0-10" data-namespace="table-ns5179"><span>11</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1040-0-11" data-namespace="table-ns5179"><span>12</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1040-1-0" data-namespace="table-ns5179"><span>GFP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1040-1-1" data-namespace="table-ns5179"><span>P-Smart</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1040-1-2" data-namespace="table-ns5179"><span>PAM-C</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1040-1-3" data-namespace="table-ns5179"><span>TBT_C</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1040-1-4" data-namespace="table-ns5179"><span>TAT-C</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1040-1-5" data-namespace="table-ns5179"><span>BadA-C</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1040-1-6" data-namespace="table-ns5179"><span>ProD-1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1040-1-7" data-namespace="table-ns5179"><span>ProD-2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1040-1-8" data-namespace="table-ns5179"><span>PAM-M</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1040-1-9" data-namespace="table-ns5179"><span>TBT-M</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1040-1-10" data-namespace="table-ns5179"><span>TAT-M</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1040-1-11" data-namespace="table-ns5179"><span>BadA-M</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table8</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1040" data-index="588" style="width:670px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1041" data-index="589" style="margin-left:0;">Old PCR machine on Angelica</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1042" data-index="590" style="margin-left:35px;">(98C for 30s / 50C for 30s / 72C for 4m30s) x35</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1043" data-index="591" style="margin-left:0;">Left (New PCR A) on Suzie</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1044" data-index="592" style="margin-left:35px;">(98C for 30s / 50C for 30s / 72C for 4m30s) x35</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1045" data-index="593" style="margin-left:0;">Right (New PCR B) on Brandon</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1046" data-index="594" style="margin-left:35px;">(98C for 30s / 50C for 30s / 72C for 3m30s) x40</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1047" data-index="595" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1048" data-index="596" style="margin-left:0;">Inoculations (Adam)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1049" data-index="597" style="margin-left:0;">Made liquid cultures of:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1050" data-index="598" style="margin-left:35px;">TycA (small colonies)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1051" data-index="599" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/8/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1052" data-index="600" style="margin-left:0;">Miniprep TycA Inoculations from 6/7 (Ben; Nisa; Ben)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1053" data-index="601" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1054" data-index="602" style="margin-left:35px;">Mini Prep Protocol NEB</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1055" data-index="603" style="margin-left:0;">50 uL elution used</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1056" data-index="604" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1057" data-index="605" style="margin-left:0;">Inoculation samples used:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1058" data-index="606" style="margin-left:35px;">TycA 1, 3, 4, 9</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1059" data-index="607" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1060" data-index="608" style="margin-left:0;"><span style="color:#DE5555">Concentrations:</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1061" data-index="609" style="margin-left:0;">Tested concentrations using nanodrop (Testing Concentrations Protocol)</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1062-item" data-formattable="true" data-index="610" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="610" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1062-table" data-formattable="true" data-index="610" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:565px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:545px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1062-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>TycA Sample</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1062-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1062-0-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>3</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1062-0-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>4</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1062-0-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>9</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1062-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Concentration (ng/uL)</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1062-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>13</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1062-1-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>13</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1062-1-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>9</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1062-1-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>4</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table20</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1062" data-index="610" style="width:565px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1063" data-index="611" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1064" data-index="612" style="margin-left:0;">Colony PCR: Testing overnight PCRs</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1065" data-index="613" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1066" data-index="614" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1067" data-index="615" style="margin-left:0;">Each will be run with:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1068" data-index="616" style="margin-left:35px;">GFP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1069" data-index="617" style="margin-left:35px;">Psmart</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1070" data-index="618" style="margin-left:35px;">PAM-C</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1071" data-index="619" style="margin-left:35px;">PAM-M</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>5.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1072" data-index="620" style="margin-left:35px;">TBT-C</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>6.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1073" data-index="621" style="margin-left:35px;">TBT-M</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>7.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1074" data-index="622" style="margin-left:35px;">ProD</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1075" data-index="623" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1076" data-index="624" style="margin-left:0;">Each Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1077" data-index="625" style="margin-left:0;">[98C for X sec / 50C for X sec / 72C for 3m30s] x 35</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1078" data-index="626" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1079" data-index="627" style="margin-left:0;">Old: X = 45s</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1080" data-index="628" style="margin-left:0;">Left: X = 45s</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1081" data-index="629" style="margin-left:0;">Right: X = 30s</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1082" data-index="630" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1083-item" data-formattable="true" data-index="631" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="631" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1083-table" data-formattable="true" data-index="631" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1083-0-0" data-namespace="table-ns5179" style="color:#344043;"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1083-0-1" data-namespace="table-ns5179" style="color:#344043;"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1083-0-2" data-namespace="table-ns5179" style="color:#344043;"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1083-1-0" data-namespace="table-ns5179" style="color:#344043;"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1083-1-1" data-namespace="table-ns5179" style="color:#344043;"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1083-1-2" data-namespace="table-ns5179" style="color:#344043;"><span>23</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1083-2-0" data-namespace="table-ns5179" style="color:#344043;"><span>MM</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1083-2-1" data-namespace="table-ns5179" style="color:#344043;"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1083-2-2" data-namespace="table-ns5179" style="color:#344043;"><span>287.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1083-3-0" data-namespace="table-ns5179" style="color:#344043;"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1083-3-1" data-namespace="table-ns5179" style="color:#344043;"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1083-3-2" data-namespace="table-ns5179" style="color:#344043;"><span>5.75</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1083-4-0" data-namespace="table-ns5179" style="color:#344043;"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1083-4-1" data-namespace="table-ns5179" style="color:#344043;"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1083-4-2" data-namespace="table-ns5179" style="color:#344043;"><span>5.75</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1083-5-0" data-namespace="table-ns5179" style="color:#344043;"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1083-5-1" data-namespace="table-ns5179" style="color:#344043;"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1083-5-2" data-namespace="table-ns5179" style="color:#344043;"><span>276</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1083-6-0" data-namespace="table-ns5179" style="color:#344043;"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1083-6-1" data-namespace="table-ns5179" style="color:#344043;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1083-6-2" data-namespace="table-ns5179" style="color:#344043;"><span>575</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table19</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1083" data-index="631" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1084" data-index="632" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/9/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1085" data-index="633" style="margin-left:0;">Ran Gel on Overnight PCR from 6/8</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1086" data-index="634" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1087" data-index="635" style="margin-left:35px;">Gels</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1088" data-index="636" style="margin-left:0;">Ran gels for </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1089-item" data-formattable="true" data-index="637" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:387px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Suzie 6/9</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="vVk8MOVXiXC99EJkJ7f7A43O6zb0ljQtryPrgh64-IMG_1232.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1089" data-index="637">RIGHT: [98C for 30 sec / 50C for 30 sec / 72C for 3m30s] x 35
 +
 +
NOTE: overnight caused some resideue bands to appear in the negative control
 +
 +
1. Ladder
 +
2. GFP
 +
3. Psmart
 +
4. PAM-C
 +
5. PAM-M
 +
6. TBT-C
 +
7. TBT-M
 +
8. ProD
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1091" data-index="638" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1092" data-index="639" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1093" data-index="640" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1094-item" data-formattable="true" data-index="641" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Brandon 6/9</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ZGUrHCKAyz3tOL6Is9KoauAehMvi2urJzZl9hpXE-IMG_1229.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1094" data-index="641">OLD: [98C for 45 sec / 50C for 45 sec / 72C for 3m30s] x 35
 +
 +
NOTE: Faint band on negative control likely due to overnights. What is the long crap on PAM-C?
 +
 +
1. Ladder
 +
2. GFP
 +
3. Psmart
 +
4. PAM-C
 +
5. PAM-M
 +
6. TBT-C
 +
7. TBT-M
 +
8. ProD
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1096" data-index="642" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1097" data-index="643" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1098" data-index="644" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1099" data-index="645" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1100-item" data-formattable="true" data-index="646" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Angelica 6/9</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="MoNRIYIzt04vGqGRg5eT1ZXr0lQaDA8JEtNhjSOJ-IMG_1227.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1100" data-index="646">LEFT: [98C for 45 sec / 50C for 45 sec / 72C for 3m30s] x 35
 +
 +
NOTE: Bright band on negative control likely due to overnight at 105C. What is the bright band over ProD?
 +
 +
1. Ladder
 +
2. GFP
 +
3. Psmart
 +
4. PAM-C
 +
5. PAM-M
 +
6. TBT-C
 +
7. TBT-M
 +
8. ProD
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1102" data-index="647" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/10/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1103" data-index="648" style="margin-left:0;">Gibson Attempt Three (Changing concentrations)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1104" data-index="649" style="margin-left:0;">We found that the lucigen p-smart is 742ng/ul </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1105" data-index="650" style="margin-left:0;">Our DBAT gblock is 40ng/ul </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1106" data-index="651" style="margin-left:0;">We diluted the p-smart at 17ng/ul in a separate aliquot </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1107" data-index="652" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1108" data-index="653" style="margin-left:0;">Attempted the gibson again, used Comp Cells from the Lynch Lab; 1 uL of DNA 20 uL of comp cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1109" data-index="654" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1110" data-index="655" style="margin-left:0;">DBAT in several trials</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1111" data-index="656" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1112" data-index="657" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1113" data-index="658" style="margin-left:35px;">Gibson Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1114" data-index="659" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 6/11/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1115" data-index="660" style="margin-left:0;"><span style="color:#DE5555">Transformation Results</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1116" data-index="661" style="margin-left:35px;"><span style="color:#DE5555">Nothing grew 😞    😭</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1117" data-index="662" style="margin-left:35px;"><span style="color:#DE5555">Potentially due to low times on the electroporation</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1118" data-index="663" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/13/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1119" data-index="664" style="margin-left:0;">Transformation Take Two</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1120" data-index="665" style="margin-left:0;">We performed the Gibson Again, on DBAT</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1121" data-index="666" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1122" data-index="667" style="margin-left:0;">Used two variables; </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1123" data-index="668" style="margin-left:35px;">4 uL of DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1124" data-index="669" style="margin-left:35px;">And then diluted with water to 1 uL of H2O and 1 uL of DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1125" data-index="670" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1126" data-index="671" style="margin-left:0;"><span style="color:#DE5555">Results (6/14)</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1127" data-index="672" style="margin-left:0;"><span style="color:#DE5555">Most plates grew</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1128" data-index="673" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/14/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1129" data-index="674" style="margin-left:0;">Colony PCR of DBAT Plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1130" data-index="675" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1131" data-index="676" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1132" data-index="677" style="margin-left:0;">Edits:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1133" data-index="678" style="margin-left:35px;">8 colonies picked per plate (DBAT -A, DBAT-B, DBAT-3)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1134" data-index="679" style="margin-left:35px;">Controls: (GFP, PSmart, TBT-M, TBT-C)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1135" data-index="680" style="margin-left:35px;">PCR run on old machine</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1136" data-index="681" style="margin-left:70px;">(98 C for 30s/ 50C for 30s/72c for 2m45s)x35</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1137" data-index="682" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1138" data-index="683" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1139-item" data-formattable="true" data-index="684" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="684" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1139-table" data-formattable="true" data-index="684" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1139-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1139-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1139-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1139-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1139-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1139-1-2" data-namespace="table-ns5179"><span>30</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1139-2-0" data-namespace="table-ns5179"><span>MM</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1139-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1139-2-2" data-namespace="table-ns5179"><span>375</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1139-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1139-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1139-3-2" data-namespace="table-ns5179"><span>7.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1139-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1139-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1139-4-2" data-namespace="table-ns5179"><span>7.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1139-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1139-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1139-5-2" data-namespace="table-ns5179"><span>360</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table21</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1139" data-index="684" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1140" data-index="685" style="margin-left:0;">Gel of colony PCR</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1141" data-index="686" style="margin-left:0;">Angelica</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1142-item" data-formattable="true" data-index="687" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_1235.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ep0hhZaK6DUtJ5dGzj9cCwpbOHcXi5iK5nZnTWlI-IMG_1235.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1142" data-index="687">1. Ladder
 +
2-5. GFP, pSMART, TBT Miniprep, TBT Miniprep
 +
6-13. DBAT-B (colonies 1-8)
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1144" data-index="688" style="margin-left:0;">Brandon </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1145-item" data-formattable="true" data-index="689" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_1236.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="YNO18wjeeIx8ZfrKW5zSKIxeOxOMYb8YE6fvC06Z-IMG_1236.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1145" data-index="689">1. Ladder
 +
2-9. DBAT-A (colonies 1-8)
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1147" data-index="690" style="margin-left:0;">Suzie</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1148-item" data-formattable="true" data-index="691" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_1239.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="YOEz2kpBISG3gQ3AYGHHgwsNG1CyeTtfte6cWRwe-IMG_1239.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1148" data-index="691">1. Ladder
 +
2-9. DBAT-3 (colonies 1-8)
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1150" data-index="692" style="margin-left:0;">Restriction Digest</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1151" data-index="693" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1152" data-index="694" style="margin-left:35px;">Digest</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1153" data-index="695" style="margin-left:0;">Edits:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1154" data-index="696" style="margin-left:35px;">Digested pSB1C3 (45 ng/uL) and TAT9 (121ng/uL) with EcoRI-HF and PstI-HF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1155" data-index="697" style="margin-left:35px;">Added DpnI in the Master Mix for pSB1C3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1156" data-index="698" style="margin-left:35px;">Mixed 3 uL of insert DNA with 5 uL of Master mix instead of 4 uL and 4 uL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1157" data-index="699" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1158" data-index="700" style="margin-left:0;">Ligation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1159" data-index="701" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1160" data-index="702" style="margin-left:35px;">Ligation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1161" data-index="703" style="margin-left:0;">Edits:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1162" data-index="704" style="margin-left:35px;">Ligated EcoRI/PstI digested pSB1C3 with <span style="color:#344043"><span style="background-color:#FFFFFF">EcoRI/PstI digested TAT9</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1163" data-index="705" style="margin-left:35px;">2 uL of each were used</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1164" data-index="706" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1165" data-index="707" style="margin-left:0;">Transformation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1166" data-index="708" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1167" data-index="709" style="margin-left:35px;">Electroporation Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1168" data-index="710" style="margin-left:0;">Edits:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1169" data-index="711" style="margin-left:35px;">3uL of ligation DNA was used as well as 20 uL of competent cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1170" data-index="712" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/15/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1171" data-index="713" style="margin-left:0;">Colony PCR of DBAT small colonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1172" data-index="714" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1173" data-index="715" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1174" data-index="716" style="margin-left:0;">DBAT-A 1:11</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1175" data-index="717" style="margin-left:0;">DBAT-B 1:4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1176" data-index="718" style="margin-left:0;">DBAT-3 1:8, 12</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1177-item" data-formattable="true" data-index="719" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="719" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1177-table" data-formattable="true" data-index="719" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1177-0-0" data-namespace="table-ns5179" style="color:#344043;"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1177-0-1" data-namespace="table-ns5179" style="color:#344043;"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1177-0-2" data-namespace="table-ns5179" style="color:#344043;"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1177-1-0" data-namespace="table-ns5179" style="color:#344043;"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1177-1-1" data-namespace="table-ns5179" style="color:#344043;"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1177-1-2" data-namespace="table-ns5179" style="color:#344043;"><span>28</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1177-2-0" data-namespace="table-ns5179" style="color:#344043;"><span>EconoTaq</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1177-2-1" data-namespace="table-ns5179" style="color:#344043;"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1177-2-2" data-namespace="table-ns5179" style="color:#344043;"><span>350</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1177-3-0" data-namespace="table-ns5179" style="color:#344043;"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1177-3-1" data-namespace="table-ns5179" style="color:#344043;"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1177-3-2" data-namespace="table-ns5179" style="color:#344043;"><span>7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1177-4-0" data-namespace="table-ns5179" style="color:#344043;"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1177-4-1" data-namespace="table-ns5179" style="color:#344043;"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1177-4-2" data-namespace="table-ns5179" style="color:#344043;"><span>7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1177-5-0" data-namespace="table-ns5179" style="color:#344043;"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1177-5-1" data-namespace="table-ns5179" style="color:#344043;"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1177-5-2" data-namespace="table-ns5179" style="color:#344043;"><span>336</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1177-6-0" data-namespace="table-ns5179" style="color:#344043;"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1177-6-1" data-namespace="table-ns5179" style="color:#344043;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1177-6-2" data-namespace="table-ns5179" style="color:#344043;"><span>700</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table22</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1177" data-index="719" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1178" data-index="720" style="margin-left:0;">Ran Gels: </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1179" data-index="721" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1180" data-index="722" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1181" data-index="723" style="margin-left:0;">pic order:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1182" data-index="724" style="margin-left:0;">angelica</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1183" data-index="725" style="margin-left:0;">brandon</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1184" data-index="726" style="margin-left:0;">suzie</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1185" data-index="727" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1186-item" data-formattable="true" data-index="728" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">image-2.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="6RhUBIwbdtXrPOEZBY98I9e7WZM4YnLbjWvywd33-image-2.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1186" data-index="728">
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1188" data-index="729" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1189" data-index="730" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1190-item" data-formattable="true" data-index="731" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">image-3.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="b1PRIyuoAAACNDqd44w5SP7tnsSSibFRBK2A9w3y-image-3.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1190" data-index="731">
 +
</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1192-item" data-formattable="true" data-index="732" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">image-4.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="E3TSIJt4ueI2cEJ3ZrB31jM1LBhJInF0wlPhutd0-image-4.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1192" data-index="732">
 +
</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/16/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1194" data-index="733" style="margin-left:0;">Colony PCR for Small DBAT Colonies </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1195" data-index="734" style="margin-left:0;">Protocols:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1196" data-index="735" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1197" data-index="736" style="margin-left:0;">Controls:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1198" data-index="737" style="margin-left:0;">GFP x100, 2.5 uL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1199" data-index="738" style="margin-left:0;">pSMART x100, 2.5 uL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1200" data-index="739" style="margin-left:0;">TBT Colony</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1201" data-index="740" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1202" data-index="741" style="margin-left:0;">Colonies:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1203" data-index="742" style="margin-left:0;">Kan 6/7/16 DBAT A 75 uL: 1, 2, 3, 4, 5, 7, 8, 9</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1204" data-index="743" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1205-item" data-formattable="true" data-index="744" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="744" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1205-table" data-formattable="true" data-index="744" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1205-0-0" data-namespace="table-ns5179" style="color:#344043;"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1205-0-1" data-namespace="table-ns5179" style="color:#344043;"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1205-0-2" data-namespace="table-ns5179" style="color:#344043;"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1205-1-0" data-namespace="table-ns5179" style="color:#344043;"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1205-1-1" data-namespace="table-ns5179" style="color:#344043;"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1205-1-2" data-namespace="table-ns5179" style="color:#344043;"><span>12</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1205-2-0" data-namespace="table-ns5179" style="color:#344043;"><span>EconoTaq</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1205-2-1" data-namespace="table-ns5179" style="color:#344043;"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1205-2-2" data-namespace="table-ns5179" style="color:#344043;"><span>150</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1205-3-0" data-namespace="table-ns5179" style="color:#344043;"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1205-3-1" data-namespace="table-ns5179" style="color:#344043;"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1205-3-2" data-namespace="table-ns5179" style="color:#344043;"><span>3</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1205-4-0" data-namespace="table-ns5179" style="color:#344043;"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1205-4-1" data-namespace="table-ns5179" style="color:#344043;"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1205-4-2" data-namespace="table-ns5179" style="color:#344043;"><span>3</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1205-5-0" data-namespace="table-ns5179" style="color:#344043;"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1205-5-1" data-namespace="table-ns5179" style="color:#344043;"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1205-5-2" data-namespace="table-ns5179" style="color:#344043;"><span>144</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1205-6-0" data-namespace="table-ns5179" style="color:#344043;"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1205-6-1" data-namespace="table-ns5179" style="color:#344043;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1205-6-2" data-namespace="table-ns5179" style="color:#344043;"><span>300</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table23</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1205" data-index="744" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1206" data-index="745" style="margin-left:0;">Ran Gels: </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1207" data-index="746" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1208" data-index="747" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1209" data-index="748" style="margin-left:0;">Inoculated DBAT</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1210" data-index="749" style="margin-left:0;">DBAT A6 came back sequence confirmed.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1211" data-index="750" style="margin-left:0;">It was inoculated in 5 mL of LB + KAN</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1212" data-index="751" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1213" data-index="752" style="margin-left:0;">Checked gBlock Concentrations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1214" data-index="753" style="margin-left:0;">Diluted to 4x (0.5 uL gBlock and 1.5uL pH20. Vol total = 2uL)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1215" data-index="754" style="margin-left:0;">Multiplied read by 4 to get the following concentrations</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1216-item" data-formattable="true" data-index="755" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="755" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1216-table" data-formattable="true" data-index="755" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:265px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:245px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-bold is-alignLeft" data-cid="table-note-part1216-0-0" data-namespace="table-ns5179"><span>gBlock</span></td><td class="cell is-text is-bold is-alignLeft" data-cid="table-note-part1216-0-1" data-namespace="table-ns5179"><span>Real Concentration (ng/uL)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1216-1-0" data-namespace="table-ns5179"><span>TycA-1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1216-1-1" data-namespace="table-ns5179"><span>48</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1216-2-0" data-namespace="table-ns5179"><span>TycA-2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1216-2-1" data-namespace="table-ns5179"><span>40</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1216-3-0" data-namespace="table-ns5179"><span>Tax10</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1216-3-1" data-namespace="table-ns5179"><span>96</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1216-4-0" data-namespace="table-ns5179"><span>BAPT</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1216-4-1" data-namespace="table-ns5179"><span>32</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table24</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1216" data-index="755" style="width:265px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1217" data-index="756" style="margin-left:0;">Freezer Stocks/Minipreping/Concentration Check/Sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1218" data-index="757" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1219" data-index="758" style="margin-left:0;">Protocols:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1220" data-index="759" style="margin-left:35px;">Freezer Stocks Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1221" data-index="760" style="margin-left:35px;">Miniprep Protocol Zyppy</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1222" data-index="761" style="margin-left:35px;">Testing Miniprep Concentration</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1223" data-index="762" style="margin-left:35px;">Preparation for Sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1224" data-index="763" style="margin-left:0;">Preformed on:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1225" data-index="764" style="margin-left:35px;">TAT9-pSB1C3 1-1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1226" data-index="765" style="margin-left:35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">TAT9-pSB1C3 1-2</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1227" data-index="766" style="margin-left:35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">TAT9-pSB1C3 2-1</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1228" data-index="767" style="margin-left:35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">TAT9-pSB1C3 2-2</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1229" data-index="768" style="margin-left:35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">TAT9-pSB1C3 2-3</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1230" data-index="769" style="margin-left:35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">TAT9-pSB1C3 2-4</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1231" data-index="770" style="margin-left:70px;">Note that there is a slim chance that 2-3 and 2-4 may have been switched within the minipreping procedure</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1232" data-index="771" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1233" data-index="772" style="margin-left:0;"><span style="color:#D10E0E">Concentration Results:</span></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1234-item" data-formattable="true" data-index="773" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="773" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1234-table" data-formattable="true" data-index="773" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:765px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:745px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1234-0-0" data-namespace="table-ns5179" style="color:#D10E0E;"><span>Sample</span></td><td class="cell is-text is-alignCenter" data-cid="table-note-part1234-0-1" data-namespace="table-ns5179" style="color:#D10E0E;"><span>1-1</span></td><td class="cell is-text is-alignCenter" data-cid="table-note-part1234-0-2" data-namespace="table-ns5179" style="color:#D10E0E;"><span>1-2</span></td><td class="cell is-text is-alignCenter" data-cid="table-note-part1234-0-3" data-namespace="table-ns5179" style="color:#D10E0E;"><span>2-1</span></td><td class="cell is-text is-alignCenter" data-cid="table-note-part1234-0-4" data-namespace="table-ns5179" style="color:#D10E0E;"><span>2-2</span></td><td class="cell is-text is-alignCenter" data-cid="table-note-part1234-0-5" data-namespace="table-ns5179" style="color:#D10E0E;"><span>2-3</span></td><td class="cell is-text is-alignCenter" data-cid="table-note-part1234-0-6" data-namespace="table-ns5179" style="color:#D10E0E;"><span>2-4</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1234-1-0" data-namespace="table-ns5179" style="color:#D10E0E;"><span>Concentration (ng/uL)</span></td><td class="cell is-value is-alignCenter" data-cid="table-note-part1234-1-1" data-namespace="table-ns5179" style="color:#D10E0E;"><span>128</span></td><td class="cell is-value is-alignCenter" data-cid="table-note-part1234-1-2" data-namespace="table-ns5179" style="color:#D10E0E;"><span>85</span></td><td class="cell is-value is-alignCenter" data-cid="table-note-part1234-1-3" data-namespace="table-ns5179" style="color:#D10E0E;"><span>160</span></td><td class="cell is-value is-alignCenter" data-cid="table-note-part1234-1-4" data-namespace="table-ns5179" style="color:#D10E0E;"><span>207</span></td><td class="cell is-value is-alignCenter" data-cid="table-note-part1234-1-5" data-namespace="table-ns5179" style="color:#D10E0E;"><span>140</span></td><td class="cell is-value is-alignCenter" data-cid="table-note-part1234-1-6" data-namespace="table-ns5179" style="color:#D10E0E;"><span>53</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table25</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1234" data-index="773" style="width:720px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1235" data-index="774" style="margin-left:0;"><span style="color:#000000">Sequencing:</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1236" data-index="775" style="margin-left:35px;">Used primers meant for the pSB1C3 backbone (Amp 1, Amp 2)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1237" data-index="776" style="margin-left:70px;">Amp 1 Concentration: 51.2 µM</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1238" data-index="777" style="margin-left:70px;"><span style="color:#344043"><span style="background-color:#FFFFFF">Amp 2 Concentration: 47.99 µM</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1239" data-index="778" style="margin-left:35px;"><span style="color:#D10E0E">Results (6/17): All were sequence confirmed except for <span style="background-color:#FFFFFF">TAT9-pSB1C3 1-2</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1240" data-index="779" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1241" data-index="780" style="margin-left:0;">Compontent Cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1242" data-index="781" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1243" data-index="782" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1244" data-index="783" style="margin-left:35px;">Electrocompetent Cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1245" data-index="784" style="margin-left:0;">Edits:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1246" data-index="785" style="margin-left:35px;">Incorrently measured OD using 420nm instead of 600nm</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1247" data-index="786" style="margin-left:35px;">Prematurely took out cells resulting in only one tube of comp. cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1248" data-index="787" style="margin-left:35px;">Redo 6/17</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1249" data-index="788" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1250" data-index="789" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/17/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1251" data-index="790" style="margin-left:0;">Control PCR</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1252" data-index="791" style="margin-left:0;">Everyone to do their own PCR to check for human error. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1253" data-index="792" style="margin-left:0;">GFP/pSM/TBT-M/TBT-C/PAM-M/PAM-C/ProD/ProD</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1254" data-index="793" style="margin-left:0;">Make own master mix - focus on what you are doing and why </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1255" data-index="794" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1256" data-index="795" style="margin-left:0;">Emma</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1257" data-index="796" style="margin-left:0;">Master Mix:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1258" data-index="797" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1259-item" data-formattable="true" data-index="798" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="798" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1259-table" data-formattable="true" data-index="798" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1259-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1259-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1259-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1259-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1259-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1259-1-2" data-namespace="table-ns5179"><span>10</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1259-2-0" data-namespace="table-ns5179"><span>EconoTaq</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1259-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1259-2-2" data-namespace="table-ns5179"><span>125</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1259-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1259-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1259-3-2" data-namespace="table-ns5179"><span>2.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1259-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1259-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1259-4-2" data-namespace="table-ns5179"><span>2.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1259-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1259-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1259-5-2" data-namespace="table-ns5179"><span>120</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1259-6-0" data-namespace="table-ns5179"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1259-6-1" data-namespace="table-ns5179"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1259-6-2" data-namespace="table-ns5179"><span>250</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table26</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1259" data-index="798" style="width:365px;margin-left:0;">H20 1st; SL1 2nd; SR2 3rd; ET 4th
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1260" data-index="799" style="margin-left:0;">Samples:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1261" data-index="800" style="margin-left:0;">1=GFP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1262" data-index="801" style="margin-left:0;">2=pSMART</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1263" data-index="802" style="margin-left:0;">3=TBT Miniprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1264" data-index="803" style="margin-left:0;">4=TBT Colony</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1265" data-index="804" style="margin-left:0;">5=Pam Miniprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1266" data-index="805" style="margin-left:0;">6=PAM Colony</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1267" data-index="806" style="margin-left:0;">7=ProD 1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1268" data-index="807" style="margin-left:0;">8=ProD2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1269" data-index="808" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1270" data-index="809" style="margin-left:0;">Master Mix was mixing by pipetting up and down</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1271" data-index="810" style="margin-left:0;">The Master Mix was not aliquoted until all four rows were being aliquoted simultaneously</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1272" data-index="811" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1273" data-index="812" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1274" data-index="813" style="margin-left:0;">Parth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1275" data-index="814" style="margin-left:0;">Samples: </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1276" data-index="815" style="margin-left:35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">pSMART miniprep 1:100 dilution</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1277" data-index="816" style="margin-left:35px;">GFP miniprep 1:100 dilution</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1278" data-index="817" style="margin-left:35px;">TBT miniprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1279" data-index="818" style="margin-left:35px;">TBT colony</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1280" data-index="819" style="margin-left:35px;">PAM miniprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1281" data-index="820" style="margin-left:35px;">PAM colony</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1282" data-index="821" style="margin-left:35px;">ProD colony 1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1283" data-index="822" style="margin-left:35px;">ProD colony 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1284" data-index="823" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1285" data-index="824" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1286" data-index="825" style="margin-left:35px;">Colony PCR Protocol</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1287-item" data-formattable="true" data-index="826" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="826" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1287-table" data-formattable="true" data-index="826" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1287-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1287-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1287-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1287-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1287-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1287-1-2" data-namespace="table-ns5179"><span>9</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1287-2-0" data-namespace="table-ns5179"><span>EconoTaq</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1287-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1287-2-2" data-namespace="table-ns5179"><span>112.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1287-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1287-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1287-3-2" data-namespace="table-ns5179"><span>2.25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1287-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1287-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1287-4-2" data-namespace="table-ns5179"><span>2.25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1287-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1287-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1287-5-2" data-namespace="table-ns5179"><span>108</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1287-6-0" data-namespace="table-ns5179"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1287-6-1" data-namespace="table-ns5179"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1287-6-2" data-namespace="table-ns5179"><span>225</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table28</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1287" data-index="826" style="width:365px;margin-left:0;">Reaction Mix
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1288" data-index="827" style="margin-left:0;">Edits to procedure: No LB was aliquoted and used, the purpose of this test was to check consistency of PCR procedure. Longest amplificant is PAM (2.5 kb); therefore, extension time was 3.5 minutes.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1289" data-index="828" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1290" data-index="829" style="margin-left:0;"><span style="color:#D10E0E">Results:</span></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1291-item" data-formattable="true" data-index="830" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_1939.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="7ow9X9nRYNQRTFdTt8jbUEnIM2QSMVQ4WtRg96Xj-IMG_1939.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1291" data-index="830">Samples going in the same order from left to right as listed previously. Note: ProD colony 1 did not PCR correctly as there was no liquid, thus there is a gap before ProD-2.
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1293" data-index="831" style="margin-left:0;"><span style="color:#D10E0E">All sizes match correctly.</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1294" data-index="832" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1295" data-index="833" style="margin-left:0;">Jay</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1296" data-index="834" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1297" data-index="835" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1298" data-index="836" style="margin-left:35px;">Colony PCR Protocol (Edit: Extension time 3 1/2 minutes)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1299" data-index="837" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1300-item" data-formattable="true" data-index="838" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="838" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1300-table" data-formattable="true" data-index="838" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1300-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1300-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1300-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1300-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1300-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1300-1-2" data-namespace="table-ns5179"><span>9</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1300-2-0" data-namespace="table-ns5179"><span>EconoTaq</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1300-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1300-2-2" data-namespace="table-ns5179"><span>112.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1300-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1300-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1300-3-2" data-namespace="table-ns5179"><span>2.25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1300-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1300-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1300-4-2" data-namespace="table-ns5179"><span>2.25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1300-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1300-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1300-5-2" data-namespace="table-ns5179"><span>108</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1300-6-0" data-namespace="table-ns5179"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1300-6-1" data-namespace="table-ns5179"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1300-6-2" data-namespace="table-ns5179"><span>225</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table27</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1300" data-index="838" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1301-item" data-formattable="true" data-index="839" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">061716Gel.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="NM4DjhAoavgq58rzOJOMd96QpHwqude034iQdXO7-061716Gel.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1301" data-index="839">1. Ladder
 +
2. Blank (Ladder contaminated)
 +
3. GFP 1:100 dilution
 +
4. pSMART 1:100 dilution
 +
5. TBT-Miniprep (50ng/uL)
 +
6. TBT-Colony
 +
<span style="color:#5C7C83"><span style="background-color:#FFFFFF">7. PAM-Miniprep (50ng/uL)
 +
8. PAM-Colony</span></span>
 +
9. ProD-Colony 1
 +
10. ProD-Colony 2
 +
11. Ladder
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1303" data-index="840" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1304" data-index="841" style="margin-left:0;">Gibsons</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1305" data-index="842" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1306" data-index="843" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1307" data-index="844" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1308" data-index="845" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1309-item" data-formattable="true" data-index="846" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">image-5.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="e3dGBLgydyqPjDyKR01ra1mSPc3dzSBiYNaChABF-image-5.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1309" data-index="846">
 +
</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 6/18/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1311" data-index="847" style="margin-left:0;">Competent Cell Test</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1312" data-index="848" style="margin-left:0;">Used 5 concentrations of DNA in 2 transformations each for a total of transformations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1313" data-index="849" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1314" data-index="850" style="margin-left:0;">White (W) = 0.5 pg/ul</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1315" data-index="851" style="margin-left:0;">Red (R) = 5 pg/ul</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1316" data-index="852" style="margin-left:0;">Green (G) = 10 pg/ul</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1317" data-index="853" style="margin-left:0;">Orange (O) = 20 pg/ul</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1318" data-index="854" style="margin-left:0;">Yellow (Y) = 50 pg/ul</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1319" data-index="855" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1320" data-index="856" style="margin-left:0;">Transformed Cells using the Electroporation protocol.  Used 1 uL of DNA in 20 ul of Comp Cells. Plated after recovering for three hours.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1321" data-index="857" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1322" data-index="858" style="margin-left:0;">Protocols:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1323" data-index="859" style="margin-left:35px;">Electroporation Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1324" data-index="860" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1325" data-index="861" style="margin-left:0;">Transformation Results</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1326" data-index="862" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1327-item" data-formattable="true" data-index="863" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="863" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1327-table" data-formattable="true" data-index="863" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-0-0" data-namespace="table-ns5179"><span>Sample </span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-0-1" data-namespace="table-ns5179"><span>Voltage (V)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-0-2" data-namespace="table-ns5179"><span>Time (ms)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-1-0" data-namespace="table-ns5179"><span>W1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-1-1" data-namespace="table-ns5179"><span>1710</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-1-2" data-namespace="table-ns5179"><span>3.9</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-2-0" data-namespace="table-ns5179"><span>W2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-2-1" data-namespace="table-ns5179"><span>1710</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-2-2" data-namespace="table-ns5179"><span>3.8</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-3-0" data-namespace="table-ns5179"><span>R1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-3-1" data-namespace="table-ns5179"><span>1700</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-3-2" data-namespace="table-ns5179"><span>0.7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-4-0" data-namespace="table-ns5179"><span>R2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-4-1" data-namespace="table-ns5179"><span>1710</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-4-2" data-namespace="table-ns5179"><span>654</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-5-0" data-namespace="table-ns5179"><span>G1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-5-1" data-namespace="table-ns5179"><span>1720</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-5-2" data-namespace="table-ns5179"><span>4.1</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-6-0" data-namespace="table-ns5179"><span>G2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-6-1" data-namespace="table-ns5179"><span>1720</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-6-2" data-namespace="table-ns5179"><span>4.3</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-7-0" data-namespace="table-ns5179"><span>O1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-7-1" data-namespace="table-ns5179"><span>1700</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-7-2" data-namespace="table-ns5179"><span>0.7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">9</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-8-0" data-namespace="table-ns5179"><span>O2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-8-1" data-namespace="table-ns5179"><span>1710</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-8-2" data-namespace="table-ns5179"><span>654</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">10</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-9-0" data-namespace="table-ns5179"><span>Y1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-9-1" data-namespace="table-ns5179"><span>1710</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-9-2" data-namespace="table-ns5179"><span>3.8</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">11</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1327-10-0" data-namespace="table-ns5179"><span>Y2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-10-1" data-namespace="table-ns5179"><span>1700</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1327-10-2" data-namespace="table-ns5179"><span>654</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table30</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1327" data-index="863" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1328" data-index="864" style="margin-left:0;">Plated each transformation on a CM plate with 25 ul of transformed cells. The Y1 and Y2 samples were plated three times at dilutions of 25 ul, 75 ul, and 100 ul.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1329" data-index="865" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1330" data-index="866" style="margin-left:0;"><span style="color:#DE5555">Results (6.19) </span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1331" data-index="867" style="margin-left:0;"><span style="color:#DE5555">The colonies on each plate were counted and tabulated. The data was then transformed using the CFU equation. </span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1332" data-index="868" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1333-item" data-formattable="true" data-index="869" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="869" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1333-table" data-formattable="true" data-index="869" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:265px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:245px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1333-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1333-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>Colony #</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1333-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>W1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1333-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>2</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1333-2-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>W2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1333-2-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>4</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1333-3-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>R1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1333-3-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>14</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1333-4-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>R2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1333-4-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1333-5-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>G1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1333-5-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>80</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1333-6-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>G2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1333-6-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>115</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1333-7-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>O1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1333-7-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>9</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">9</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1333-8-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>O2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1333-8-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>38</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table31</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1333" data-index="869" style="width:265px;margin-left:0;">Colonies/Plate for all but Y1 and Y2
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1334" data-index="870" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1335-item" data-formattable="true" data-index="871" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="871" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1335-table" data-formattable="true" data-index="871" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1335-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Dilution</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1335-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>Y1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1335-0-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>Y2</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part1335-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1335-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>20</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1335-1-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>52</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part1335-2-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>75</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1335-2-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>53</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1335-2-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>29</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part1335-3-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>100</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1335-3-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>33</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1335-3-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>233</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table32</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1335" data-index="871" style="width:365px;margin-left:0;">Colonies/Plate of Yellow Concentration
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1336" data-index="872" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1337-item" data-formattable="true" data-index="873" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="873" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1337-table" data-formattable="true" data-index="873" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:608px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:588px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:143px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>Colony #</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-0-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>Dilution (ul)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-0-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>DNA Concentration (ng/ul)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-0-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>CFU</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>W1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-1-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-1-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.005</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-1-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>10000000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-2-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>W2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-2-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>4</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-2-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-2-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.005</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-2-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>20000000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-3-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>R1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-3-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>14</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-3-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-3-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.005</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-3-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>70000000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-4-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>R2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-4-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>6</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-4-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-4-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.005</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-4-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>30000000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-5-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>G1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-5-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>80</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-5-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-5-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.01</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-5-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>200000000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-6-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>G2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-6-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>115</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-6-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-6-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.01</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-6-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>287500000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-7-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>O1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-7-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>9</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-7-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-7-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.02</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-7-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>11250000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">9</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-8-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>O2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-8-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>38</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-8-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-8-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.02</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-8-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>47500000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">10</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-9-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Y1a</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-9-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>20</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-9-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-9-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.05</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-9-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>10000000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">11</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-10-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Y1b</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-10-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>53</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-10-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>75</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-10-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.05</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-10-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>79500000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">12</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-11-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Y1c</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-11-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>33</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-11-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>100</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-11-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.05</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-11-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>66000000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">13</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-12-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Y2a</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-12-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>52</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-12-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-12-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.05</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-12-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>26000000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">14</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-13-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Y2b</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-13-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>29</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-13-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>75</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-13-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.05</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-13-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>43500000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">15</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-14-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Y2c</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-14-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>233</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-14-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>100</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-14-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>0.05</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-14-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>466000000</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">16</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-15-0" data-namespace="table-ns5179"><span>Average</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-15-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-15-2" data-namespace="table-ns5179" style="color:#DE5555;"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1337-15-3" data-namespace="table-ns5179" style="color:#DE5555;"><span></span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1337-15-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>97660714.2857142836</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table33</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1337" data-index="873" style="width:608px;margin-left:0;">Transformed Data
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1338" data-index="874" style="margin-left:0;">Plates Chilled</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1339" data-index="875" style="margin-left:0;">Plates in the incubator were taken out </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1340" data-index="876" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1341" data-index="877" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1342" data-index="878" style="margin-left:0;">Colony PCR</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1343" data-index="879" style="margin-left:0;">Emma</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1344" data-index="880" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">samples (color, letter/number)</span></span></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1345-item" data-formattable="true" data-index="881" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="881" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1345-table" data-formattable="true" data-index="881" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1345-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1345-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1345-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1345-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1345-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1345-1-2" data-namespace="table-ns5179"><span>17</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1345-2-0" data-namespace="table-ns5179"><span>EconoTaq</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1345-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1345-2-2" data-namespace="table-ns5179"><span>212.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1345-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1345-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1345-3-2" data-namespace="table-ns5179"><span>4.25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1345-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1345-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1345-4-2" data-namespace="table-ns5179"><span>4.25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1345-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1345-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1345-5-2" data-namespace="table-ns5179"><span>204</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1345-6-0" data-namespace="table-ns5179"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1345-6-1" data-namespace="table-ns5179"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1345-6-2" data-namespace="table-ns5179"><span>425</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table34</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1345" data-index="881" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1346" data-index="882" style="margin-left:0;">Thomas</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1347" data-index="883" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">samples (color, letter/number)</span></span></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1348-item" data-formattable="true" data-index="884" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="884" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1348-table" data-formattable="true" data-index="884" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1348-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1348-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1348-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1348-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1348-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1348-1-2" data-namespace="table-ns5179"><span>35</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1348-2-0" data-namespace="table-ns5179"><span>EconoTaq</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1348-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1348-2-2" data-namespace="table-ns5179"><span>437.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1348-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1348-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1348-3-2" data-namespace="table-ns5179"><span>8.75</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1348-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1348-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1348-4-2" data-namespace="table-ns5179"><span>8.75</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1348-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1348-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1348-5-2" data-namespace="table-ns5179"><span>420</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1348-6-0" data-namespace="table-ns5179"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1348-6-1" data-namespace="table-ns5179"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1348-6-2" data-namespace="table-ns5179"><span>875</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table36</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1348" data-index="884" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1349" data-index="885" style="margin-left:0;">Parth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1350" data-index="886" style="margin-left:0;">samples (color, letter/number)</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1351-item" data-formattable="true" data-index="887" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="887" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1351-table" data-formattable="true" data-index="887" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1351-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1351-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1351-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1351-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1351-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1351-1-2" data-namespace="table-ns5179"><span>35</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1351-2-0" data-namespace="table-ns5179"><span>EconoTaq</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1351-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1351-2-2" data-namespace="table-ns5179"><span>437.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1351-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1351-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1351-3-2" data-namespace="table-ns5179"><span>8.75</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1351-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1351-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1351-4-2" data-namespace="table-ns5179"><span>8.75</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1351-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1351-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1351-5-2" data-namespace="table-ns5179"><span>420</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1351-6-0" data-namespace="table-ns5179"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1351-6-1" data-namespace="table-ns5179"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1351-6-2" data-namespace="table-ns5179"><span>875</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table35</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1351" data-index="887" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1352" data-index="888" style="margin-left:0;">PCR 30@98C/30@50C/3:00@72C</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1353" data-index="889" style="margin-left:0;">Controls: GFP, psmart, TBT-M, TBT-C, ProD</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1354" data-index="890" style="margin-left:0;">Master Mix for Emma's control made by Thomas</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1355" data-index="891" style="margin-left:0;">PCR machine for Emma's Samples was turned off after ten seconds due to Jay's forgotten samples</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1356" data-index="892" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-subheader1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1357" data-index="893" style="margin-left:0;">Gel Electrophoresis</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1358-item" data-formattable="true" data-index="894" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="894" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1358-table" data-formattable="true" data-index="894" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:796px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:776px;"><colgroup><col style="width:45px;"></col><col style="width:53px;"></col><col style="width:44px;"></col><col style="width:48px;"></col><col style="width:56px;"></col><col style="width:90px;"></col><col style="width:77px;"></col><col style="width:100px;"></col><col style="width:82px;"></col><col style="width:87px;"></col><col style="width:94px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">H</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">I</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">J</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-0-0" data-namespace="table-ns5179"><span>Well 1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-0-1" data-namespace="table-ns5179"><span>Well 2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-0-2" data-namespace="table-ns5179"><span>Well 3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-0-3" data-namespace="table-ns5179"><span>Well 4</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-0-4" data-namespace="table-ns5179"><span>Well 5</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-0-5" data-namespace="table-ns5179"><span>Well 6</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-0-6" data-namespace="table-ns5179"><span>Wells 7-26</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-0-7" data-namespace="table-ns5179"><span>Well 27</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-0-8" data-namespace="table-ns5179"><span>Wells 28-35</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-0-9" data-namespace="table-ns5179"><span>Wells 36-50</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-1-0" data-namespace="table-ns5179"><span>GFP (PP)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-1-1" data-namespace="table-ns5179"><span>ProD (PP)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-1-2" data-namespace="table-ns5179"><span>TBT-Miniprep (PP)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-1-3" data-namespace="table-ns5179"><span>TBT-Colony (PP)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-1-4" data-namespace="table-ns5179"><span>PSmart (PP)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-1-5" data-namespace="table-ns5179"><span>Ladder</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-1-6" data-namespace="table-ns5179"><span>Green Numbers 1-20; BAPT #2 (TL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-1-7" data-namespace="table-ns5179"><span>Ladder</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-1-8" data-namespace="table-ns5179"><span>Red Letters A-H; TAX10 #1 (TL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1358-1-9" data-namespace="table-ns5179"><span>Black Numbers 1-15; BAPT #1 (JCS)</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table37</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1358" data-index="894" style="width:720px;margin-left:0;">Big Bertha Row 1
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1359" data-index="895" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1360" data-index="896" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1361-item" data-formattable="true" data-index="897" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="897" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1361-table" data-formattable="true" data-index="897" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:902px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:882px;"><colgroup><col style="width:45px;"></col><col style="width:53px;"></col><col style="width:44px;"></col><col style="width:48px;"></col><col style="width:56px;"></col><col style="width:90px;"></col><col style="width:77px;"></col><col style="width:100px;"></col><col style="width:82px;"></col><col style="width:87px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">H</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">I</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">J</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">K</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-0-0" data-namespace="table-ns5179"><span>Well 1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-0-1" data-namespace="table-ns5179"><span>Well 2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-0-2" data-namespace="table-ns5179"><span>Well 3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-0-3" data-namespace="table-ns5179"><span>Well 4</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-0-4" data-namespace="table-ns5179"><span>Well 5</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-0-5" data-namespace="table-ns5179"><span>Wells 6-29</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-0-6" data-namespace="table-ns5179"><span>Wells 30-43</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-0-7" data-namespace="table-ns5179"><span>Well 44</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-0-8" data-namespace="table-ns5179"><span>Wells 45-48</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-0-9" data-namespace="table-ns5179"><span>Well 49</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-0-10" data-namespace="table-ns5179"><span>Well 50</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-1-0" data-namespace="table-ns5179"><span>GFP (EM)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-1-1" data-namespace="table-ns5179"><span>PSmart (EM)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-1-2" data-namespace="table-ns5179"><span>TBT-Colony (EM)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-1-3" data-namespace="table-ns5179"><span>TBT-Miniprep (EM)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-1-4" data-namespace="table-ns5179"><span>Ladder</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-1-5" data-namespace="table-ns5179"><span>Green Letters A-X; BAPT #2 (EM)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-1-6" data-namespace="table-ns5179"><span>Black Letters A-N; BAPT #1 (EM)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-1-7" data-namespace="table-ns5179"><span>Ladder</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-1-8" data-namespace="table-ns5179"><span>Blue Letters A-D; TAX10 #2 (EM)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-1-9" data-namespace="table-ns5179"><span>(Evaporated) Intended for Blue Letter E; TAX10 #2 (EM)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1361-1-10" data-namespace="table-ns5179"><span>Blue Letter F; TAX10 #2 (EM)</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table38</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1361" data-index="897" style="width:720px;margin-left:0;">Big Bertha Row 2
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1362" data-index="898" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1363-item" data-formattable="true" data-index="899" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Big Bertha.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="C1AxqPpvFZhzb9wHvIfo4La04kdbvh2y5fxNtj0j-Big_Bertha.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1363" data-index="899">See Tables above
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1365" data-index="900" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1366" data-index="901" style="margin-left:0;">Angelica</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1367-item" data-formattable="true" data-index="902" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Angelica.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="xSGNm2WZZE9aVHLrG9hDVRJjEDgMN7z5K78z1DiY-Angelica.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1367" data-index="902">1. GFP (Version: Thomas's)
 +
2. pSMART <span style="color:#5C7C83"><span style="background-color:#FFFFFF">(Version: Thomas's)</span></span>
 +
3. TBT-Miniprep <span style="color:#5C7C83"><span style="background-color:#FFFFFF">(Version: Thomas's)</span></span>
 +
4. TBT-Colony <span style="color:#5C7C83"><span style="background-color:#FFFFFF">(Version: Thomas's)</span></span>
 +
5. ProD <span style="color:#5C7C83"><span style="background-color:#FFFFFF">(Version: Thomas's)</span></span>
 +
6. Ladder
 +
7-13. TAX10#2 1-7 (Blue Numbers)
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1369" data-index="903" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1370" data-index="904" style="margin-left:0;">Brandon</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1371-item" data-formattable="true" data-index="905" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Brandon.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="77XhwIU3Ohe4i6dwZei08iNJRLBMuzE39ZXCs2XT-Brandon.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1371" data-index="905">1. Ladder
 +
2-4. TAX10#2 8-10 (Blue Numbers)
 +
5. ProD (Version: Emma's)
 +
6-13. TAX10#1 1-8 (Red Numbers)
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1373" data-index="906" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1374" data-index="907" style="margin-left:0;">Suzie</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1375-item" data-formattable="true" data-index="908" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Suzie.JPG</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="cIlFFaqr4fIyM9c7TikhAUjbwdKmdGhrtLA8ITfz-Suzie.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1375" data-index="908">1. Ladder
 +
2-9. TAX10#1 10-17 (Red Numbers)
 +
10. Blank
 +
11. TAX10#1 9 (Red Number)
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1377" data-index="909" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/21</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1378" data-index="910" style="margin-left:0;">Minipreps/Concentration Check/Sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1379" data-index="911" style="margin-left:0;">Protocols:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1380" data-index="912" style="margin-left:35px;">Miniprep Protocol Zyppy</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1381" data-index="913" style="margin-left:35px;">Testing Miniprep Concentration</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1382" data-index="914" style="margin-left:35px;">Preparation for Sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1383" data-index="915" style="margin-left:0;">Samples:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1384" data-index="916" style="margin-left:70px;"><span style="color:#344043"><span style="background-color:#FFFFFF">BAPT #1  (A, B)</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1385" data-index="917" style="margin-left:70px;"><span style="color:#344043"><span style="background-color:#FFFFFF">TAX10 #2 (2, 3, 4)</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1386" data-index="918" style="margin-left:70px;">TAX10 #2 (A, F)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1387" data-index="919" style="margin-left:70px;"><span style="color:#344043"><span style="background-color:#FFFFFF">BAPT #2 (C, D, F, R, X)</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1388" data-index="920" style="margin-left:0;"><span style="color:#D10E0E">Results</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1389" data-index="921" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1390-item" data-formattable="true" data-index="922" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="922" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1390-table" data-formattable="true" data-index="922" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:963px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:943px;"><colgroup><col style="width:45px;"></col><col style="width:53px;"></col><col style="width:73px;"></col><col style="width:66px;"></col><col style="width:67px;"></col><col style="width:70px;"></col><col style="width:70px;"></col><col style="width:68px;"></col><col style="width:68px;"></col><col style="width:72px;"></col><col style="width:71px;"></col><col style="width:72px;"></col><col style="width:70px;"></col><col style="width:78px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">H</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">I</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">J</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">K</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">L</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">M</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Sample</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT#1-A</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT#1-B</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT#2-C</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT#2-D</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT#2-F</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT#2-R</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-7" data-namespace="table-ns5179" style="color:#DE5555;"><span>BAPT#2-X</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-8" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10#2-A</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-9" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10#2-F</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-10" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10#2-2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-11" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10#2-3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-0-12" data-namespace="table-ns5179" style="color:#DE5555;"><span>TAX10#2-4</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1390-1-0" data-namespace="table-ns5179" style="color:#DE5555;"><span>Conc</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1390-1-1" data-namespace="table-ns5179" style="color:#DE5555;"><span>123</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1390-1-2" data-namespace="table-ns5179" style="color:#DE5555;"><span>60</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1390-1-3" data-namespace="table-ns5179" style="color:#DE5555;"><span>67</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1390-1-4" data-namespace="table-ns5179" style="color:#DE5555;"><span>61</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1390-1-5" data-namespace="table-ns5179" style="color:#DE5555;"><span>66</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1390-1-6" data-namespace="table-ns5179" style="color:#DE5555;"><span>56</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1390-1-7" data-namespace="table-ns5179" style="color:#DE5555;"><span>54</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1390-1-8" data-namespace="table-ns5179" style="color:#DE5555;"><span>50</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1390-1-9" data-namespace="table-ns5179" style="color:#DE5555;"><span>52</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1390-1-10" data-namespace="table-ns5179" style="color:#DE5555;"><span>49</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1390-1-11" data-namespace="table-ns5179" style="color:#DE5555;"><span>54</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1390-1-12" data-namespace="table-ns5179" style="color:#DE5555;"><span>41</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table42</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1390" data-index="922" style="width:720px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1391" data-index="923" style="margin-left:0;">Sent in for sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1392" data-index="924" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/22</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1393" data-index="925" style="margin-left:0;">Colony PCR on TycA </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1394" data-index="926" style="margin-left:0;">4:30 elongation 30 s Elongation/Denaturation</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1395-item" data-formattable="true" data-index="927" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="927" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1395-table" data-formattable="true" data-index="927" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1395-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1395-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1395-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1395-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1395-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1395-1-2" data-namespace="table-ns5179"><span>53</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1395-2-0" data-namespace="table-ns5179"><span>EconoTaq</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1395-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1395-2-2" data-namespace="table-ns5179"><span>662.5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1395-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1395-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1395-3-2" data-namespace="table-ns5179"><span>13.25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1395-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1395-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1395-4-2" data-namespace="table-ns5179"><span>13.25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1395-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1395-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1395-5-2" data-namespace="table-ns5179"><span>636</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1395-6-0" data-namespace="table-ns5179"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1395-6-1" data-namespace="table-ns5179"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1395-6-2" data-namespace="table-ns5179"><span>1325</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table43</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1395" data-index="927" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1396" data-index="928" style="margin-left:0;">Gel Electrophoresis for TycA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1397" data-index="929" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1398" data-index="930" style="margin-left:0;">Angelica</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1399-item" data-formattable="true" data-index="931" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">TycA62216Angelica.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Xpd5lfuSFmMvDhOwUo2cHvZmXdnOPiUiNPEvS15N-TycA62216Angelica.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1399" data-index="931">1. Ladder
 +
2. GFP
 +
3. pSMART
 +
4-10: TycA yi 1-7
 +
11-13: TycA san 11-13
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1401" data-index="932" style="margin-left:0;">Brandon</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1402-item" data-formattable="true" data-index="933" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">TycA62216Brandon.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="1rjNjsC1w0JQDLraR7hrmRcwCTK80s55NqhMs9YR-TycA62216Brandon.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1402" data-index="933">1. Ladder
 +
2. TBT Mini Prep
 +
3. TBT Colony
 +
4-11. TycA er 1-8
 +
12. Empty
 +
13. TycA san 14
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1404" data-index="934" style="margin-left:0;">Suzie</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1405-item" data-formattable="true" data-index="935" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">TycA62216Suzie.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="TZHMRqPcYCclMXjABD15K2LUYPbnGb13x45jjucP-TycA62216Suzie.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1405" data-index="935">1. Ladder
 +
2. PAM Mini Prep
 +
3. PAM Colony
 +
4-11. TycA #2 1-8
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1407" data-index="936" style="margin-left:0;">Mike</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1408-item" data-formattable="true" data-index="937" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">TycA62216Mike.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="diYUhX0zm9kDk9JrFG3BQmRVnUnN7CsiBHsmr9ho-TycA62216Mike.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1408" data-index="937">1. Ladder
 +
2. ProD
 +
3. ProD
 +
4-13. TycA san 1-10
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1410" data-index="938" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1411" data-index="939" style="margin-left:0;">Inoculations were made of samples:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1412" data-index="940" style="margin-left:35px;">TycA san: 1, 10</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1413" data-index="941" style="margin-left:35px;">TycA er: 6, 8</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1414" data-index="942" style="margin-left:35px;">TycA yi: 4, 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1415" data-index="943" style="margin-left:35px;">TycA #2: 4, 5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1416" data-index="944" style="margin-left:0;">Inoculations placed in 5 mL of LB+KAN and</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1417" data-index="945" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1418" data-index="946" style="margin-left:0;">Transformations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1419" data-index="947" style="margin-left:0;">Transformed several samples into cells. All samples used 1ul of DNA and 20 ul of Comp Cells.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1420" data-index="948" style="margin-left:0;">Transformations were recovered for 2 hours and then 75 uL was plated onto a KAN Plate. Another set of plates was made after 4 hours of recovery with a 5:95 ratio of cells to LB.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1421" data-index="949" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1422-item" data-formattable="true" data-index="950" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="950" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1422-table" data-formattable="true" data-index="950" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:465px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:445px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-0-0" data-namespace="table-ns5179"><span>DNA Sample</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-0-1" data-namespace="table-ns5179"><span>Cell Sample</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-0-2" data-namespace="table-ns5179"><span>Volts</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-0-3" data-namespace="table-ns5179"><span>Tims (ms)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-1-0" data-namespace="table-ns5179"><span>DBAT #1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-1-1" data-namespace="table-ns5179"><span>286</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-1-2" data-namespace="table-ns5179"><span>1700</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-1-3" data-namespace="table-ns5179"><span>3.7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-2-0" data-namespace="table-ns5179"><span>DBAT #2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-2-1" data-namespace="table-ns5179"><span>286</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-2-2" data-namespace="table-ns5179"><span>1710</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-2-3" data-namespace="table-ns5179"><span>3.8</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-3-0" data-namespace="table-ns5179"><span>DBAT EV #1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-3-1" data-namespace="table-ns5179"><span>286</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-3-2" data-namespace="table-ns5179"><span>1700</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-3-3" data-namespace="table-ns5179"><span>3.6</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-4-0" data-namespace="table-ns5179"><span>DBAT EV #2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-4-1" data-namespace="table-ns5179"><span>286</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-4-2" data-namespace="table-ns5179"><span>1710</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-4-3" data-namespace="table-ns5179"><span>3.8</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-5-0" data-namespace="table-ns5179"><span>BadA EV #1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-5-1" data-namespace="table-ns5179"><span>286</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-5-2" data-namespace="table-ns5179"><span>1710</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-5-3" data-namespace="table-ns5179"><span>3.7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-6-0" data-namespace="table-ns5179"><span>BadA EV #2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-6-1" data-namespace="table-ns5179"><span>286</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-6-2" data-namespace="table-ns5179"><span>1710</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-6-3" data-namespace="table-ns5179"><span>3.7</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-7-0" data-namespace="table-ns5179"><span>DBAT #1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-7-1" data-namespace="table-ns5179"><span>E.Cloni</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-7-2" data-namespace="table-ns5179"><span>1720</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-7-3" data-namespace="table-ns5179"><span>4</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">9</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-8-0" data-namespace="table-ns5179"><span>DBAT #2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1422-8-1" data-namespace="table-ns5179"><span>E.Cloni</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-8-2" data-namespace="table-ns5179"><span>1720</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1422-8-3" data-namespace="table-ns5179"><span>4</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table44</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1422" data-index="950" style="width:465px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1423" data-index="951" style="margin-left:0;">Restriction Digest</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1424" data-index="952" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1425" data-index="953" style="margin-left:35px;">Digest</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1426" data-index="954" style="margin-left:0;">Edits:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1427" data-index="955" style="margin-left:35px;">Digested pSB1C3 (45 ng/uL) and DBAT (70ng/uL) with EcoRI-HF and PstI-HF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1428" data-index="956" style="margin-left:35px;">Added DpnI in the Master Mix for pSB1C3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1429" data-index="957" style="margin-left:35px;">Mixed 3 uL of insert DNA with 5 uL of Master mix instead of 4 uL and 4 uL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1430" data-index="958" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1431" data-index="959" style="margin-left:0;">Ligation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1432" data-index="960" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1433" data-index="961" style="margin-left:35px;">Ligation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1434" data-index="962" style="margin-left:0;">Edits:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1435" data-index="963" style="margin-left:35px;">Ligated EcoRI/PstI digested pSB1C3 with <span style="color:#344043"><span style="background-color:#FFFFFF">EcoRI/PstI digested DBAT</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1436" data-index="964" style="margin-left:35px;">2 uL of each were used</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1437" data-index="965" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1438" data-index="966" style="margin-left:0;">Transformation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1439" data-index="967" style="margin-left:0;">Protocol:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1440" data-index="968" style="margin-left:35px;">Electroporation Protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1441" data-index="969" style="margin-left:0;">Edits:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1442" data-index="970" style="margin-left:35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">3uL of ligation DNA was used as well as 20 uL of competent cells</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1443" data-index="971" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1444" data-index="972" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1445" data-index="973" style="margin-left:0;">Minipreps/Concentration Check/Sequencing (Nisa)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1446" data-index="974" style="margin-left:0;">Protocols:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1447" data-index="975" style="margin-left:35px;">Miniprep Protocol Zyppy</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1448" data-index="976" style="margin-left:35px;">Testing Miniprep Concentration</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1449" data-index="977" style="margin-left:35px;">Preparation for Sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1450" data-index="978" style="margin-left:0;">Samples:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1451" data-index="979" style="margin-left:35px;">TycA EV-6</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1452" data-index="980" style="margin-left:35px;">TycA San 1, 10</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1453" data-index="981" style="margin-left:0;"><span style="color:#D10E0E">Results</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1454" data-index="982" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1455-item" data-formattable="true" data-index="983" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="983" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1455-table" data-formattable="true" data-index="983" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:465px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:445px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1455-0-0" data-namespace="table-ns5179" style="color:#D10E0E;"><span>Sample</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1455-0-1" data-namespace="table-ns5179" style="color:#D10E0E;"><span>TycA EV-6</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1455-0-2" data-namespace="table-ns5179" style="color:#D10E0E;"><span>TycA San-1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1455-0-3" data-namespace="table-ns5179" style="color:#D10E0E;"><span>TycA San-10</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1455-1-0" data-namespace="table-ns5179" style="color:#D10E0E;"><span>Conc</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1455-1-1" data-namespace="table-ns5179" style="color:#D10E0E;"><span>41</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1455-1-2" data-namespace="table-ns5179" style="color:#D10E0E;"><span>9</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1455-1-3" data-namespace="table-ns5179" style="color:#D10E0E;"><span>55</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table45</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1455" data-index="983" style="width:465px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1456" data-index="984" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Made Plates of 286 (Parth &amp; Nisa)</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1457" data-index="985" style="margin-left:0;">Made plates of 286 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1458" data-index="986" style="margin-left:35px;">1 with 25 uL concentration spread out</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1459" data-index="987" style="margin-left:35px;">2 by streaking into single colonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1460" data-index="988" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1461" data-index="989" style="margin-left:0;">Inoculations (Parth)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1462" data-index="990" style="margin-left:0;">Made Inoculations of DBAT-pSB1C3 #1 (A-E)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1463" data-index="991" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/24</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1464" data-index="992" style="margin-left:0;">Freezer Stocks/Miniprep/Concentration Check/Sequencing (Nisa)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1465" data-index="993" style="margin-left:0;">Protocols:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1466" data-index="994" style="margin-left:35px;">Freezer Stocks Protocol (750 uL)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1467" data-index="995" style="margin-left:35px;">Miniprep Protocol Zyppy (50 uL elution buffer)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1468" data-index="996" style="margin-left:35px;">Testing Miniprep Concentrations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1469" data-index="997" style="margin-left:35px;">Preparation for Sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1470" data-index="998" style="margin-left:0;">Samples:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1471" data-index="999" style="margin-left:35px;"><span style="color:#344043"><span style="background-color:#FFFFFF">DBAT-pSB1C3 #1 (A-E)</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1472" data-index="1000" style="margin-left:0;"><span style="color:#D10E0E">Results</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1473" data-index="1001" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1474-item" data-formattable="true" data-index="1002" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="1002" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1474-table" data-formattable="true" data-index="1002" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:651px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:631px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:86px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1474-0-0" data-namespace="table-ns5179" style="color:#D10E0E;"><span>Sample</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1474-0-1" data-namespace="table-ns5179" style="color:#D10E0E;"><span>DBAT-pSB1C3#1-A</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1474-0-2" data-namespace="table-ns5179" style="color:#D10E0E;"><span>DBAT-pSB1C3#1-B</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1474-0-3" data-namespace="table-ns5179" style="color:#D10E0E;"><span>DBAT-pSB1C3#1-C</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1474-0-4" data-namespace="table-ns5179" style="color:#D10E0E;"><span>DBAT-pSB1C3#1-D</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1474-0-5" data-namespace="table-ns5179" style="color:#D10E0E;"><span>DBAT-pSB1C3#1-E</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1474-1-0" data-namespace="table-ns5179" style="color:#D10E0E;"><span>Conc</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1474-1-1" data-namespace="table-ns5179" style="color:#D10E0E;"><span>31</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1474-1-2" data-namespace="table-ns5179" style="color:#D10E0E;"><span>84</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1474-1-3" data-namespace="table-ns5179" style="color:#D10E0E;"><span>51</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1474-1-4" data-namespace="table-ns5179" style="color:#D10E0E;"><span>47</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1474-1-5" data-namespace="table-ns5179" style="color:#D10E0E;"><span>56</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table29</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1474" data-index="1002" style="width:651px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1475" data-index="1003" style="margin-left:0;">Gibson Transformations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1476" data-index="1004" style="margin-left:0;">Samples:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1477" data-index="1005" style="margin-left:35px;">TAX10-1B</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1478" data-index="1006" style="margin-left:35px;">TAX10-2B</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1479" data-index="1007" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1480" data-index="1008" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1481" data-index="1009" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 6/25</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1482" data-index="1010" style="margin-left:0;">Colony PCR on TAX10 Plates 1B &amp; 2B</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1483" data-index="1011" style="margin-left:0;">3:00 elongation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1484" data-index="1012" style="margin-left:0;">38 Reactions: 17 colonies from 1B, 13 colonies from 2B, 1 uL GFP, 1 uL pSMART, 1 uL TBT miniprep, 1 TBT colony, 1 uL BadA miniprep, 1 BadA colony, 2 ProD colonies</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1485-item" data-formattable="true" data-index="1013" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="1013" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1485-table" data-formattable="true" data-index="1013" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1485-0-0" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1485-0-1" data-namespace="table-ns5179"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1485-0-2" data-namespace="table-ns5179"><span>(# col +1 control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1485-1-0" data-namespace="table-ns5179"><span>Thing</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1485-1-1" data-namespace="table-ns5179"><span>1 rxn</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1485-1-2" data-namespace="table-ns5179"><span>40</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1485-2-0" data-namespace="table-ns5179"><span>EconoTaq</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1485-2-1" data-namespace="table-ns5179"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1485-2-2" data-namespace="table-ns5179"><span>500</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1485-3-0" data-namespace="table-ns5179"><span>SL1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1485-3-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1485-3-2" data-namespace="table-ns5179"><span>10</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1485-4-0" data-namespace="table-ns5179"><span>SR2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1485-4-1" data-namespace="table-ns5179"><span>0.25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1485-4-2" data-namespace="table-ns5179"><span>10</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1485-5-0" data-namespace="table-ns5179"><span>H2O</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1485-5-1" data-namespace="table-ns5179"><span>12</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1485-5-2" data-namespace="table-ns5179"><span>480</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1485-6-0" data-namespace="table-ns5179"><span>Total</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1485-6-1" data-namespace="table-ns5179"><span>25</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part1485-6-2" data-namespace="table-ns5179"><span>1000</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table39</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1485" data-index="1013" style="width:365px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-subheader1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1486" data-index="1014" style="margin-left:0;">Gel Electrophoresis</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-subheader1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1487" data-index="1015" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1488" data-index="1016" style="margin-left:0;">Angelica</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1489" data-index="1017" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1490-item" data-formattable="true" data-index="1018" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="1018" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1490-table" data-formattable="true" data-index="1018" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:356px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:336px;"><colgroup><col style="width:45px;"></col><col style="width:53px;"></col><col style="width:44px;"></col><col style="width:48px;"></col><col style="width:56px;"></col><col style="width:90px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1490-0-0" data-namespace="table-ns5179"><span>Well 1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1490-0-1" data-namespace="table-ns5179"><span>Well 2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1490-0-2" data-namespace="table-ns5179"><span>Well 3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1490-0-3" data-namespace="table-ns5179"><span>Wells 4-12</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1490-0-4" data-namespace="table-ns5179"><span>Well 13</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1490-1-0" data-namespace="table-ns5179"><span>Ladder</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1490-1-1" data-namespace="table-ns5179"><span>GFP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1490-1-2" data-namespace="table-ns5179"><span>pSMART</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1490-1-3" data-namespace="table-ns5179"><span>TAX10 Plate 1B #1-9</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1490-1-4" data-namespace="table-ns5179"><span>Blank</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table40</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1490" data-index="1018" style="width:356px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1491-item" data-formattable="true" data-index="1019" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Angelica62516.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="YD3vZZM1yikZi4mOiIk4rE6bfFfYZRIuzYQz5Tkq-Angelica62516.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1491" data-index="1019">
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1493" data-index="1020" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1494" data-index="1021" style="margin-left:0;">Brandon</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1495" data-index="1022" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1496-item" data-formattable="true" data-index="1023" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="1023" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1496-table" data-formattable="true" data-index="1023" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:356px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:336px;"><colgroup><col style="width:45px;"></col><col style="width:53px;"></col><col style="width:44px;"></col><col style="width:48px;"></col><col style="width:56px;"></col><col style="width:90px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1496-0-0" data-namespace="table-ns5179"><span>Well 1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1496-0-1" data-namespace="table-ns5179"><span>Well 2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1496-0-2" data-namespace="table-ns5179"><span>Well 3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1496-0-3" data-namespace="table-ns5179"><span>Wells 4-11</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1496-0-4" data-namespace="table-ns5179"><span>Wells 12-13</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1496-1-0" data-namespace="table-ns5179"><span>Ladder</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1496-1-1" data-namespace="table-ns5179"><span>TBT Miniprep</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1496-1-2" data-namespace="table-ns5179"><span>TBT Colony</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1496-1-3" data-namespace="table-ns5179"><span>TAX10 Plate 1B #10-17</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1496-1-4" data-namespace="table-ns5179"><span>Blank</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table41</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1496" data-index="1023" style="width:356px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1497-item" data-formattable="true" data-index="1024" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Brandon62516.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="8SioCIjknKMKrwkHP2UR120biHQb8bC6jU0zxfKe-Brandon62516.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1497" data-index="1024">
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1499" data-index="1025" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1500" data-index="1026" style="margin-left:0;">Suzie</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1501" data-index="1027" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1502-item" data-formattable="true" data-index="1028" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="1028" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1502-table" data-formattable="true" data-index="1028" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:356px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:336px;"><colgroup><col style="width:45px;"></col><col style="width:53px;"></col><col style="width:44px;"></col><col style="width:48px;"></col><col style="width:56px;"></col><col style="width:90px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1502-0-0" data-namespace="table-ns5179"><span>Well 1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1502-0-1" data-namespace="table-ns5179"><span>Wells 2-3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1502-0-2" data-namespace="table-ns5179"><span>Wells 4-10</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1502-0-3" data-namespace="table-ns5179"><span>Well 11</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1502-0-4" data-namespace="table-ns5179"><span>Wells 12-13</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1502-1-0" data-namespace="table-ns5179"><span>Ladder</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1502-1-1" data-namespace="table-ns5179"><span>ProD Colonies</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1502-1-2" data-namespace="table-ns5179"><span>TAX10 Plate 2B #1-7</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1502-1-3" data-namespace="table-ns5179"><span>Blank</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1502-1-4" data-namespace="table-ns5179"><span>TAX10 Plate 2B #5-6</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table46</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1502" data-index="1028" style="width:356px;margin-left:0;">
 +
</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1503-item" data-formattable="true" data-index="1029" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Suzie62516.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="QbHSBtIXo90nOUQaL7hNlLSG2gL0HCjkH1bN74wm-Suzie62516.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1503" data-index="1029">
 +
</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1505" data-index="1030" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1506" data-index="1031" style="margin-left:0;">Mike</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1507" data-index="1032" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1508-item" data-formattable="true" data-index="1033" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="1033" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1508-table" data-formattable="true" data-index="1033" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:556px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:536px;"><colgroup><col style="width:45px;"></col><col style="width:53px;"></col><col style="width:44px;"></col><col style="width:48px;"></col><col style="width:56px;"></col><col style="width:90px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-0-0" data-namespace="table-ns5179"><span>Well 1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-0-1" data-namespace="table-ns5179"><span>Well 2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-0-2" data-namespace="table-ns5179"><span>Well 3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-0-3" data-namespace="table-ns5179"><span>Wells 4-5</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-0-4" data-namespace="table-ns5179"><span>Well 6</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-0-5" data-namespace="table-ns5179"><span>Wells 7-10</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-0-6" data-namespace="table-ns5179"><span>Wells 11-13</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-1-0" data-namespace="table-ns5179"><span>Ladder</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-1-1" data-namespace="table-ns5179"><span>BadA Miniprep</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-1-2" data-namespace="table-ns5179"><span>BadA Colony</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-1-3" data-namespace="table-ns5179"><span>TAX10 Plate 2B #8-9</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-1-4" data-namespace="table-ns5179"><span>Blank</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-1-5" data-namespace="table-ns5179"><span>TAX10 Plate 2B #10-13</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part1508-1-6" data-namespace="table-ns5179"><span>Blank</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table47</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1508" data-index="1033" style="width:556px;margin-left:0;">
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</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1509-item" data-formattable="true" data-index="1034" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Mike62516.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Ttv8ZZHdY9FWIgGh75fdjw2c5BtL52iDMn8yvYuf-Mike62516.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1509" data-index="1034">
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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1511" data-index="1035" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/28</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1512" data-index="1036" style="margin-left:0;">Digest/Ligation/Transformation of TAX10+ pSB1C3 (Parth)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1513" data-index="1037" style="margin-left:0;">Protocols:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1514" data-index="1038" style="margin-left:35px;">Digest</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1515" data-index="1039" style="margin-left:70px;">Edits: <span style="color:#344043"><span style="background-color:#FFFFFF">2uL pSB1C3+2uL dH2O, 1uL TAX10+3uLdH2O to make the amounts equimolar for the ligation</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1516" data-index="1040" style="margin-left:70px;">TAX10 taken was directly from gBlock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1517" data-index="1041" style="margin-left:35px;">Ligation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1518" data-index="1042" style="margin-left:70px;">Two attempts at ligation (Samples: #1, #2)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1519" data-index="1043" style="margin-left:35px;">Transformation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>○</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1520" data-index="1044" style="margin-left:70px;">Edits: Plated Sample #1 @ 75uL and 25uL, Plated Sample #2 once @ 75 uL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1521" data-index="1045" style="margin-left:0;"><span style="color:#D10E0E">Result: Failure, no colonies grew</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1522" data-index="1046" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1523" data-index="1047" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header1"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1524" data-index="1048" style="margin-left:0;">Splitting pSmart into Pieces before Gibson</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-header2"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1525" data-index="1049" style="margin-left:0;">PCR</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1526" data-index="1050" style="margin-left:35px;">2 reactions: 1) linear pSmart (17 ng/uL), 1/2 forward primer, 1/2 reverse primer; 2) <span style="color:#344043"><span style="background-color:#FFFFFF">linear pSmart (17 ng/uL), 2/2 forward primer, 2/2 reverse primer</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1527" data-index="1051" style="margin-left:35px;">63 degrees Celsius annealing temperature</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1528" data-index="1052" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1529-item" data-formattable="true" data-index="1053" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="1053" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1529-table" data-formattable="true" data-index="1053" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:465px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:445px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-num
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Latest revision as of 19:56, 19 October 2016

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