Difference between revisions of "Team:Dundee/Experiments"

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{{Dundee}}
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<html lang="en">
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<p>Describe the experiments, research and protocols you used in your iGEM project.</p>
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<div class="column half_size">
 
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<h5>What should this page contain?</h5>
 
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<ul>
 
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<li> Protocols </li>
 
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<li> Experiments </li>
 
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<li>Documentation of the development of your project </li>
 
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</ul>
 
  
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</div>
 
  
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<h5>Inspiration</h5>
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<ul>
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#sideMenu, #top_title {display:none;}
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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  <body>
 +
 
 +
 
 +
 
 +
<!-- Fixed navbar -->
 +
    <nav class="navbar navbar-default navbar-fixed-top">
 +
      <div style="padding-right:5%; padding-bottom:0px;" "class="container">
 +
        <div class="navbar-header">
 +
          <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false" aria-controls="navbar">
 +
            <span class="sr-only">Toggle navigation</span>
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            <span class="icon-bar"></span>
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            <span class="icon-bar"></span>
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            <span class="icon-bar"></span>
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 +
          <a href="https://2016.igem.org/Team:Dundee" class="navbar-brand">UoD iGEM 2016</a>
 +
        </div>
 +
        <div id="navbar" class="navbar-collapse collapse">
 +
          <ul class="nav navbar-nav">
 +
            <li class="dropdown">
 +
  <a class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Project <span class="caret"></span></a>
 +
              <ul class="dropdown-menu">
 +
                <li><a href="https://2016.igem.org/Team:Dundee/Description">Description</a></li>
 +
                <li><a href="https://2016.igem.org/Team:Dundee/Design">Design</a></li>
 +
                <li><a href="https://2016.igem.org/Team:Dundee/Results">Results</a></li>
 +
<li><a href="https://2016.igem.org/Team:Dundee/Hardware">Hardware</a></li>
 +
              </ul>
 +
</li>
 +
            <li ><a href="https://2016.igem.org/Team:Dundee/Model">Modelling</a></li>
 +
<li><a href="https://2016.igem.org/Team:Dundee/Proof">Proof of Concept</a></li>
 +
<li class="dropdown">
 +
              <a class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Human Practices <span class="caret"></span></a>
 +
              <ul class="dropdown-menu">
 +
    <li><a href="https://2016.igem.org/Team:Dundee/Human_Practices">Human Practices Overview</a></li>
 +
                <li><a href="https://2016.igem.org/Team:Dundee/Integrated_Practices">Integrated Human Practices</a></li>
 +
                <li><a href="https://2016.igem.org/Team:Dundee/HP/Silver">HP/silver</a></li>
 +
                <li><a href="https://2016.igem.org/Team:Dundee/HP/Gold">HP/gold</a></li>
 +
<li><a href="https://2016.igem.org/Team:Dundee/Engagement">Public Engagement</a></li>
 +
              </ul>
 +
            </li>
 +
<li class="dropdown">
 +
              <a class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Achievements <span class="caret"></span></a>
 +
              <ul class="dropdown-menu">
 +
                <li><a href="https://2016.igem.org/Team:Dundee/Parts">Parts</a></li>
 +
<li ><a href="https://2016.igem.org/Team:Dundee/Basic_Part">Basic Parts</a></li>
 +
                <li><a href="https://2016.igem.org/Team:Dundee/Composite_Part">Composite Parts</a></li>
 +
                <li><a href="https://2016.igem.org/Team:Dundee/Part_Collection">Parts Collection</a></li>
 +
<li><a href="https://2016.igem.org/Team:Dundee/Experiments">Awards</a></li>
 +
              </ul>
 +
            </li>
 +
<li class="dropdown">
 +
              <a class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Notebook <span class="caret"></span></a>
 +
              <ul class="dropdown-menu">
 +
<li><a href="https://2016.igem.org/Team:Dundee/Notebook">Lab Book</a></li>
 +
                <li><a href="https://2016.igem.org/Team:Dundee/Safety">Safety</a></li>
 +
              </ul>
 +
<li class="dropdown">
 +
              <a class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Team <span class="caret"></span></a>
 +
              <ul class="dropdown-menu">
 +
<li><a href="https://2016.igem.org/Team:Dundee/Team">Meet The Agents</a></li>
 +
                <li class="active"><a href="https://2016.igem.org/Team:Dundee/Attributions">Attributions</a></li>
 +
                <li><a href="https://2016.igem.org/Team:Dundee/Collaborations">Collaboration</a></li>
 +
              </ul>
 +
            </li>
 +
          </ul>
 +
        </div><!--/.nav-collapse -->
 +
      </div>
 +
    </nav>
 +
 
 +
                  <div id="BackImg">
 +
<img src="https://static.igem.org/mediawiki/2016/f/fa/T--Dundee--Medals.jpg" />
 +
</div>
 +
 
 +
   
 +
 +
 +
<div class="container">
 +
<div id = "SplashTitle">
 +
<p>Judging Criteria</p>
 +
</div>
 +
 
 +
<div id="SplashTitleUnder">
 +
<p>Dundee 2016 </p>
 +
</div>
 +
</div>
 +
 +
 +
<div class="container">
 +
<div  id= "ProjectDesCon">
 +
<h3>Bronze</h3>
 +
 +
<div id="FlaskImg1">
 +
<img src="https://static.igem.org/mediawiki/2016/f/f1/T--Dundee--BronzeMedal.png"/>
 +
</div>
 +
 +
<div id="FlaskPos1">
 +
<p>1. We have registered the team, had a great summer and plan to have fun at the Jamboree.</p>
 +
<p>2. We have met all deliverables, documented our work on the wiki page, plan to present a poster and presentation at the Jamboree.</p>
 +
<ul>
 +
<li>We have also attributed all of the work done for our project. </li>
 +
<li>We have also created and documented our Part pages on the Registry for the Parts </li>
 +
<li>We have submitted. And all part samples have been submitted to the Registry. </li>
 +
<li>We have completed the following forms: About Our Lab, About Project, Final Safety Form and the Judging Form.  </li>
 +
</ul>
 +
</li>
 +
<p>3. We have clearly attributed each aspect of our project.</p>
 +
<p>4. We have documented the following BioBrick Parts: </p>
 +
 +
<ul>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962000
 +
">BBa_K1962000
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962001
 +
">BBa_K1962001
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962002
 +
">BBa_K1962002
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962004
 +
">BBa_K1962004
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962005
 +
">BBa_K1962005
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962006
 +
">BBa_K1962006
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962015
 +
">BBa_K1962015
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962016
 +
">BBa_K1962016
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962017
 +
">BBa_K1962017
 +
</a></li>
 +
</ul>
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
<div class="container">
 +
<div  id= "ProjectDesCon">
 +
<h3>Silver</h3>
 +
 +
<div id="FlaskImg1">
 +
<img src="https://static.igem.org/mediawiki/2016/3/39/T--Dundee--SilverMedal.png"/>
 +
</div>
 +
 +
<div id="FlaskPos1">
 +
<p>1. We have experimentally validated that at least one new BioBrick Part of our own design works as expected. </p>
 +
<ul>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962003
 +
">BBa_K1962003
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962007
 +
">BBa_K1962007
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962008
 +
">BBa_K1962008
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K1962009
 +
">BBa_K1962009
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K19620010
 +
">BBa_K19620010
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K19620011
 +
">BBa_K19620011
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K19620012
 +
">BBa_K19620012
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K19620013
 +
">BBa_K19620013
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_K19620014
 +
">BBa_K19620014
 +
</a></li>
 +
</ul>
 +
<p>2. We have helped other iGEM teams by characterizing a part, mentoring a high school team and attending iGEM meetups.</p>
 +
<p>3. Human practices – silver. Identified and investigated important issues including the sustainability of antibiotics, public concerns on the ethics of using GMOs. We were also involved in organizing a debate with the Edniburgh iGEM team to tackle some misconceptions that the public had on GMOs.</p>
 +
</div>
 +
</div>
 +
</div>
 +
 
 +
<div class="container">
 +
<div  id= "ProjectDesCon">
 +
<h3>Gold</h3>
 +
 +
 +
<div id="FlaskImg1">
 +
<img src="https://static.igem.org/mediawiki/2016/4/45/T--Dundee--GoldMedal.png"/>
 +
</div>
 +
 +
<div id="FlaskPos1">
 +
 
 +
<p>1. Human practices - gold. Our initial idea was to target infections that cause food poisoning, however we gained valuable feedback from our human practices which made us think about targeting infections at the source of the problem. We also created a BactiFeed product in the lab to explore the real world applications of the device.    </p>
 +
<p>2. We have improved the characterization of a number of parts and documented them in the parts page on the registry.
 +
</p>
 +
 +
<p>- Improved the characterization of two pH sensitive promoters. We improved them by cloning GFP downstream of the two promoters and measuring GFP fluorescence in different pH conditions.
 +
</p>
 +
<ul>
 +
<li><i>Pasr</i>- <a href="http://parts.igem.org/Part:BBa_K1231000
 +
">BBa_K1231000
 +
</a> </li>
 +
<li><i>PgadA</i> – <a href="http://parts.igem.org/Part:BBa_K1231001
 +
">BBa_K1231001
 +
</a></li>
 +
<li><a href="http://parts.igem.org/Part:BBa_E0840
 +
">BBa_E0840
 +
</a></li>
 +
</ul>
 +
 +
<p>- We have also improved the characterization of the <i>P acrRA</i> . By cloning the gfp gene downstream and measuring gfp fluorescence. </p>
 +
 +
<ul>
 +
<li><i>Pasr</i>- <a href="http://parts.igem.org/Part:BBa_K1231000
 +
">BBa_K1231000
 +
</a> </li>
 +
</ul>
 +
<p>3. We have created a piece of hardware that will mimic the churning motion in the gut of a chicken in order to test our bactifeed.  </p>
 +
</div>
 +
</div>
 +
</div>
 +
 
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<div class="container">
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<div id="SponserFooter">
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<div  id = "BBSRC" class="SponserLink">
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<a href="http://www.bbsrc.ac.uk/">
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<img class="grayscale" src="https://static.igem.org/mediawiki/2016/8/8b/T--Dundee--BBSRCFooter.png" />
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</a>
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</div>
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<div  class="SponserLink">
 +
<a href="http://www.dundee.ac.uk/">
 +
<img class="grayscale" src="https://static.igem.org/mediawiki/2016/a/a8/T--Dundee--UoDFooter.png" />
 +
</a>
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</div>
 +
<div  class="SponserLink">
 +
<a href="https://www.idtdna.com/site">
 +
<img class="grayscale" src="https://static.igem.org/mediawiki/2016/0/03/T--Dundee--IDTFooter.png" />
 +
</a>
 +
</div>
 +
<div class="SponserLink">
 +
<a href="https://www.sebiology.org/">
 +
<img  class="grayscale"src="https://static.igem.org/mediawiki/2016/f/f0/T--Dundee--SEBFooter.png" />
 +
</a>
 +
</div>
 +
<div id = "WellcomeT" class="SponserLink">
 +
<a href="https://wellcome.ac.uk/">
 +
<img class="grayscale"src="https://static.igem.org/mediawiki/2016/2/22/T--Dundee--WELLTFooter.png" />
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</a>
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<a>
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<img src="https://static.igem.org/mediawiki/2016/b/be/T--Dundee--MiddleFooter.png" />
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</div>
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<div class="footerImg">
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<a  style="padding-right:0px;"href="https://twitter.com/DundeeiGEMTeam">
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<img  src="https://static.igem.org/mediawiki/2016/4/4f/T--Dundee--TWFooter.png" />
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                </a>
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Latest revision as of 20:02, 19 October 2016

Dundee 2016

Judging Criteria

Dundee 2016

Bronze

1. We have registered the team, had a great summer and plan to have fun at the Jamboree.

2. We have met all deliverables, documented our work on the wiki page, plan to present a poster and presentation at the Jamboree.

  • We have also attributed all of the work done for our project.
  • We have also created and documented our Part pages on the Registry for the Parts
  • We have submitted. And all part samples have been submitted to the Registry.
  • We have completed the following forms: About Our Lab, About Project, Final Safety Form and the Judging Form.

3. We have clearly attributed each aspect of our project.

4. We have documented the following BioBrick Parts:

Silver

1. We have experimentally validated that at least one new BioBrick Part of our own design works as expected.

2. We have helped other iGEM teams by characterizing a part, mentoring a high school team and attending iGEM meetups.

3. Human practices – silver. Identified and investigated important issues including the sustainability of antibiotics, public concerns on the ethics of using GMOs. We were also involved in organizing a debate with the Edniburgh iGEM team to tackle some misconceptions that the public had on GMOs.

Gold

1. Human practices - gold. Our initial idea was to target infections that cause food poisoning, however we gained valuable feedback from our human practices which made us think about targeting infections at the source of the problem. We also created a BactiFeed product in the lab to explore the real world applications of the device.

2. We have improved the characterization of a number of parts and documented them in the parts page on the registry.

- Improved the characterization of two pH sensitive promoters. We improved them by cloning GFP downstream of the two promoters and measuring GFP fluorescence in different pH conditions.

- We have also improved the characterization of the P acrRA . By cloning the gfp gene downstream and measuring gfp fluorescence.

3. We have created a piece of hardware that will mimic the churning motion in the gut of a chicken in order to test our bactifeed.