Difference between revisions of "Team:TU Delft/Parts"

 
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{{TU_Delft}}
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{{:Team:TU_Delft/Header}}
 
<html>
 
<html>
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    <head>
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        <meta charset="utf-8">
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        <title>iGEM TU Delft</title>
 +
        <meta name="description" content="">
 +
        <meta name="viewport" content="width=device-width, initial-scale=1">
 +
        <script type='text/javascript'        src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
 +
        <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' />
 +
        <link rel='stylesheet' type='text/css' href='/common/table_styles.css' />
 +
        <style>
 +
            #delft table.pgrouptable td{
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                font-size: 12px;
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                border-bottom: 2px solid #119ED8;
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                border-right: 1px solid #119ED8;
 +
            }
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            #delft table.pgrouptable{
  
 +
                font-size: 14px;
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                width:100%;
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            }
  
  
 +
            #delft th{
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                background-color:#119ED8;
 +
                padding: 10px;
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                color: white;
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                border:none;
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                border-bottom: 1px solid #199ED8;
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                border-collapse: collapse;
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                vertical-align: text-top;
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                text-align: left;}
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            #delft thead {
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                background: #119ED8;
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                border: none;
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                border-bottom: 1px solid #8E999D;
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                color: #f3f4f4;
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            }
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            #delft .table-style-1 td,
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            .table-style-1 th {
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                border-bottom: 1px solid #119ED8;
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                border-right: 2px solid #119ED8;
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            }
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#delft .heart13 {
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    background-image: url(https://igem.org/common/tablesorter/themes/groupparts/heart13.gif);
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    background-repeat: no-repeat;
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    background-position: center center;}
  
  
  
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<div class="column full_size">
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        </style>
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    </head>
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    <body id="delft">
 +
        <div class="page-heading text-center">
 +
            <div class="container">
 +
                <h1 class="page-header">Parts<span class="title-under"></span></h1>
 +
                <p>For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to
 +
                            generate our required parts in a fast and efficient way. All of the parts were designed containing the coding
 +
                            sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are <a href="#composite"><b>composite</b></a>.
 +
                            The different <a href="#basic"><b>basic</b></a> parts comprising the composite parts are documented seperately, although they do not exist induvidually.
 +
                            In the case of a constitutive expression, the promoter is included in the biobrick itself.
 +
                            For the inducible expression, however, we made use of a plasmid backbone containing a Lac-promoter
 +
                            and the LacI gene, which encodes for the Lac repressor. Consequently, these parts themselves do
 +
                            not contain a promoter. Additionally, using PCR rather than synthesis, we constructed a small
 +
                                <a href="#collection"><b>collection</b></a> of GFP expression devices with different promoter strengths, based on an existing biobrick.
 +
Thereby improving both the function and characterization of the original part</p>
 +
            </div>
 +
        </div>
  
 +
        <div  class="main-container">
 +
            <div class="container">
 +
                <div class="parts">
 +
                    <div class="row">
 +
                        <span class="anchor" id="parts"></span>
 +
                        <div class="container">
 +
                            <div class="col-md-8 col-md-offset-2">
 +
                                </html>
 +
                                <groupparts class="parts">iGEM2016 TU_Delft</groupparts>
 +
                                <html>
 +
                            </div>
 +
                        </div>
 +
                    </div>
 +
                   
 +
                    <div class="row">
 +
                        <span class="anchor" id="composite"></span>
 +
                        <h2 class="title-style-1">Composite Parts<span class="title-under"></span></h2>
 +
                        <p>For the construction of our parts, we made use of the IDT synthesis offer.
 +
                            This way we were able to generate our required parts in a fast and efficient way.
 +
                            All of the parts were designed containing the coding sequence, RBS, promoter
 +
                            and terminators, if applicable. Therefore, all of the parts we used are composite.</p>
 +
                       
 +
                        <div class="col-md-10 col-md-offset-1"
 +
                           
 +
                            <table>
  
−
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
                                <table class="table table-style-1 pgrouptable tablesorter">
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
                                    <thead>
 +
                                        <tr>
 +
                                            <th>Name</th>
 +
                                            <th>Type</th>
 +
                                            <th>Description</th>
 +
                                            <th>Designer</th>
 +
                                            <th>Length</th>
 +
                                        </tr>
  
 +
                                    </thead>
 +
                                    <tbody>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890000">BBa_K1890000</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>Silicatein gene with strong RBS</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">686</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890001">BBa_K1890001</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>Silicatein gene, fused to transmembrane domain of INP, with strong RBS</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">1442</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890002">BBa_K1890002</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>Silicatein gene, fused to transmembrane domain of OmpA, with strong RBS</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">1481</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890010">BBa_K1890010</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>mCerulean with strong consitutive promoter and RBS</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">784</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890011">BBa_K1890011</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>mVenus with strong consitutive promoter and RBS</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">784</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890020">BBa_K1890020</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with strong constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890021">BBa_K1890021</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with strong constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890022">BBa_K1890022</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with medium constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890023">BBa_K1890023</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with weak constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890024">BBa_K1890024</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with weak constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890030">BBa_K1890030</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>BolA gene with RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">508</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890031">BBa_K1890031</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>BolA gene with strong promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">519</td>
 +
                                        </tr>
 +
                                    </tbody>
 +
                                </table>
  
−
</div>
+
                        </div>
 +
                    </div>
 +
<div class="row">
 +
                        <span class="anchor" id="basic"></span>
 +
                        <h2 class="title-style-1">Basic Parts<span class="title-under"></span></h2>
 +
                        <p>The different basic parts comprising the synthesized parts we used are documented here.
 +
                            Please note that these parts do not exist individually.
 +
</p>
 +
                        <div class="col-md-10 col-md-offset-1"
 +
                           
 +
                            <table>
  
 +
                                <table class="table table-style-1 pgrouptable tablesorter">
 +
                                    <thead>
 +
                                        <tr>
 +
                                            <th>Name</th>
 +
                                            <th>Type</th>
 +
                                            <th>Description</th>
 +
                                            <th>Designer</th>
 +
                                            <th>Length</th>
 +
                                        </tr>
  
 +
                                    </thead>
 +
                                    <tbody>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890050">BBa_K1890050</a></td>
 +
                                            <td>Coding</td>
 +
                                            <td>Silicatein from Suberites domuncula</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">660</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890051">BBa_K1890051</a></td>
 +
                                            <td>Coding</td>
 +
                                            <td>Silicatein from Tethya aurantium</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">993</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890052">BBa_K1890052</a></td>
 +
                                            <td>Protein_Domain</td>
 +
                                            <td>OmpA transmembrane domain</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">462</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890053">BBa_K1890053</a></td>
 +
                                            <td>Protein_Domain</td>
 +
                                            <td>INP transmembrane domain</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">732</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890054">BBa_K1890054</a></td>
 +
                                            <td>Coding</td>
 +
                                            <td>mCerulean</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">720</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890055">BBa_K1890055</a></td>
 +
                                            <td>Coding</td>
 +
                                            <td>mVenus</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">720</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890056">BBa_K1890056</a></td>
 +
                                            <td>Coding</td>
 +
                                            <td>BolA</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">321</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890058">BBa_K1890058</a></td>
 +
                                            <td>DNA</td>
 +
                                            <td>Linker</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">24</td>
 +
                                        </tr>
 +
                                    </tbody>
 +
                                </table>
  
 +
                        </div>
 +
                    </div>
 +
                    <div class="row">
 +
                        <span class="anchor" id="collection"></span>
 +
                        <h2 class="title-style-1">Part Collection<span class="title-under"></span></h2>
 +
                        <p>We provided a small collection of GFP expression devices with different promoter
 +
                        strengths, which we used to study the effect of different levels of constitutive expression. These parts are
 +
                        ready-to-go expression units for green fluorescence, which can be useful for future teams.
 +
By constructing these parts, we improved both the function and the characterization of the <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_E0840" target="_blank">original part BBa_E0840</a></b>.</p>
 +
                    </div>
 +
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 +
                           
 +
                            <table>
  
 +
                                <table class="table table-style-1 pgrouptable tablesorter">
 +
                                    <thead>
 +
                                        <tr>
 +
                                            <th>Name</th>
 +
                                            <th>Type</th>
 +
                                            <th>Description</th>
 +
                                            <th>Designer</th>
 +
                                            <th>Length</th>
 +
                                        </tr>
 +
                                    </thead>
 +
                                    <tbody>
 +
                                       
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890020">BBa_K1890020</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with strong constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890021">BBa_K1890021</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with strong constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890022">BBa_K1890022</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with medium constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890023">BBa_K1890023</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with weak constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890024">BBa_K1890024</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with weak constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                    </tbody>
 +
                                </table>
  
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<div class="column half_size">
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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 +
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        </div>
 +
        <!--  Scripts================================================== -->
  
 +
        <script type="text/javascript" src="https://2016.igem.org/Template:TU_Delft/JS?action=raw&ctype=text/javascript"></script>
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        <!-- Bootsrap javascript file -->
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        <script type="text/javascript" src="https://2016.igem.org/Template:TU_Delft/BootstrapJS?action=raw&ctype=text/javascript"></script>
  
 +
<script type="text/javascript" src ="https://igem.org/common/tablesorter/jquery.tablesorter.min.js"></script>
 +
        <link rel="stylesheet" type="text/css" href="https://igem.org/common/tablesorter/themes/groupparts/style.css"/>
 +
        <link rel="stylesheet" type="text/css" href="https://igem.org/common/table_styles.css"/>
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<script type="text/javascript" src ="https://igem.org/wiki/skins/Igem/resources/2016_skin.js"></script>
  
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
+
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<ul>
+
−
<li>Part Name</li>
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−
<li>Part type</li>
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−
<li>Creator</li>
+
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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+
−
<p>
+
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<groupparts>iGEM2016 TU_Delft</groupparts>
 
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Latest revision as of 20:27, 19 October 2016

iGEM TU Delft

Parts

For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts were designed containing the coding sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are composite. The different basic parts comprising the composite parts are documented seperately, although they do not exist induvidually. In the case of a constitutive expression, the promoter is included in the biobrick itself. For the inducible expression, however, we made use of a plasmid backbone containing a Lac-promoter and the LacI gene, which encodes for the Lac repressor. Consequently, these parts themselves do not contain a promoter. Additionally, using PCR rather than synthesis, we constructed a small collection of GFP expression devices with different promoter strengths, based on an existing biobrick. Thereby improving both the function and characterization of the original part

                               <groupparts class="parts">iGEM2016 TU_Delft</groupparts>

Composite Parts

For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts were designed containing the coding sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are composite.

Name Type Description Designer Length
BBa_K1890000 Composite Silicatein gene with strong RBS Lycka Kamoen, Maria Vazquez 686
BBa_K1890001 Composite Silicatein gene, fused to transmembrane domain of INP, with strong RBS Lycka Kamoen, Maria Vazquez 1442
BBa_K1890002 Composite Silicatein gene, fused to transmembrane domain of OmpA, with strong RBS Lycka Kamoen, Maria Vazquez 1481
BBa_K1890010 Composite mCerulean with strong consitutive promoter and RBS Lycka Kamoen, Maria Vazquez 784
BBa_K1890011 Composite mVenus with strong consitutive promoter and RBS Lycka Kamoen, Maria Vazquez 784
BBa_K1890020 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890021 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890022 Generator GFP with medium constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890023 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890024 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890030 Composite BolA gene with RBS and terminator Lycka Kamoen, Maria Vazquez 508
BBa_K1890031 Composite BolA gene with strong promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 519

Basic Parts

The different basic parts comprising the synthesized parts we used are documented here. Please note that these parts do not exist individually.

Name Type Description Designer Length
BBa_K1890050 Coding Silicatein from Suberites domuncula Lycka Kamoen, Maria Vazquez 660
BBa_K1890051 Coding Silicatein from Tethya aurantium Lycka Kamoen, Maria Vazquez 993
BBa_K1890052 Protein_Domain OmpA transmembrane domain Lycka Kamoen, Maria Vazquez 462
BBa_K1890053 Protein_Domain INP transmembrane domain Lycka Kamoen, Maria Vazquez 732
BBa_K1890054 Coding mCerulean Lycka Kamoen, Maria Vazquez 720
BBa_K1890055 Coding mVenus Lycka Kamoen, Maria Vazquez 720
BBa_K1890056 Coding BolA Lycka Kamoen, Maria Vazquez 321
BBa_K1890058 DNA Linker Lycka Kamoen, Maria Vazquez 24

Part Collection

We provided a small collection of GFP expression devices with different promoter strengths, which we used to study the effect of different levels of constitutive expression. These parts are ready-to-go expression units for green fluorescence, which can be useful for future teams. By constructing these parts, we improved both the function and the characterization of the original part BBa_E0840.

Name Type Description Designer Length
BBa_K1890020 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890021 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890022 Generator GFP with medium constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890023 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890024 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913

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