Difference between revisions of "Team:TU Delft/Parts"

 
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div#groupparts{
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        <title>iGEM TU Delft</title>
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        <meta name="description" content="">
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        <script type='text/javascript'        src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
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            #delft table.pgrouptable td{
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            #delft table.pgrouptable{
  
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  <div class="jumbotron" id="cover">
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                width:100%;
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    <h1>Parts</h1>
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            }
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    <p class="lead">Below you will find all the parts listed that were created by the TU Delft team in the course of the iGEM 2015 competition.</p>
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  </div>
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<span class="anchor" id="overview"></span>
 
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  <div id="white">
 
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    <div class="jumbotron">
 
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      <div class="container">
 
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        <h1>Overview</h1>
 
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      </div>
 
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    </div>
 
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    <div class="container">
 
  
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<p class="lead">This year IDT sponsored the synthesis of 20 kb for every iGEM team. For this reason, we did not clone our basic parts into the pSB1C3 plasmid backbone, but directly combined them in the synthesis of our composite parts. However, we experienced problems with 2 out of 20 gBlock genes during cloning which unfortunately in the end had to be dropped from the project. </p>
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            #delft th{
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                background-color:#119ED8;
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                padding: 10px;
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                color: white;
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                vertical-align: text-top;
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            #delft thead {
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                border: none;
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                color: #f3f4f4;
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            #delft .table-style-1 td,
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#delft .heart13 {
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<p class="lead">Our initial plan to use GFP (BBa_I13522) to additionally color label the cells containing our plasmid DNA was dropped in favor of using RFP (I13521). In our experience the bacterial cells are intensively red when expressing this gene, which especially makes colony picking after transformation significantly more straightforward than with GFP. Based on this we want to encourage all coming iGEM teams to use RFP instead of GFP.</p>
 
  
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<p class="lead">Since our project was about the induced formation of a biofilm from amyloid nanowires, most of our parts consist of the csgA gene or are variations of it.
 
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For instance, in order to mimick the characteristic adhesiveness of biofilms we added different affinity tags to CsgA. </p>
 
  
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    <body id="delft">
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        <div class="page-heading text-center">
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            <div class="container">
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                <h1 class="page-header">Parts<span class="title-under"></span></h1>
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                <p>For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to
 +
                            generate our required parts in a fast and efficient way. All of the parts were designed containing the coding
 +
                            sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are <a href="#composite"><b>composite</b></a>.
 +
                            The different <a href="#basic"><b>basic</b></a> parts comprising the composite parts are documented seperately, although they do not exist induvidually.
 +
                            In the case of a constitutive expression, the promoter is included in the biobrick itself.
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                            For the inducible expression, however, we made use of a plasmid backbone containing a Lac-promoter
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                            and the LacI gene, which encodes for the Lac repressor. Consequently, these parts themselves do
 +
                            not contain a promoter. Additionally, using PCR rather than synthesis, we constructed a small
 +
                                <a href="#collection"><b>collection</b></a> of GFP expression devices with different promoter strengths, based on an existing biobrick.
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Thereby improving both the function and characterization of the original part</p>
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            </div>
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        </div>
  
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                <div class="parts">
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        <h1>TU Delft iGEM 2015 Parts</h1>
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                        <span class="anchor" id="parts"></span>
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        <p class="lead text-muted">Basic & Composite parts</p>
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                        <div class="container">
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      </div>
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    <div class="container">
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                                <groupparts class="parts">iGEM2016 TU_Delft</groupparts>
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                            </div>
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                    <div class="row">
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                        <span class="anchor" id="composite"></span>
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                        <h2 class="title-style-1">Composite Parts<span class="title-under"></span></h2>
 +
                        <p>For the construction of our parts, we made use of the IDT synthesis offer.
 +
                            This way we were able to generate our required parts in a fast and efficient way.
 +
                            All of the parts were designed containing the coding sequence, RBS, promoter
 +
                            and terminators, if applicable. Therefore, all of the parts we used are composite.</p>
 +
                       
 +
                        <div class="col-md-10 col-md-offset-1"
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                                <table class="table table-style-1 pgrouptable tablesorter">
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<groupparts>iGEM015 TU_Delft</groupparts>
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                                    <thead>
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                                        <tr>
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                                            <th>Name</th>
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  </div>
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                                            <th>Type</th>
 +
                                            <th>Description</th>
 +
                                            <th>Designer</th>
 +
                                            <th>Length</th>
 +
                                        </tr>
  
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<span class="anchor" id="collection"></span>
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                                    </thead>
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  <div id="white">
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                                    <tbody>
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    <div class="jumbotron">
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                                        <tr>
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      <div class="container">
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                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890000">BBa_K1890000</a></td>
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        <h1>Part Collection</h1>
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                                            <td>Composite</td>
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        <p class="lead text-muted">CsgA multitool box</p>
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                                            <td>Silicatein gene with strong RBS</td>
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      </div>
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                                            <td>Lycka Kamoen, Maria Vazquez</td>
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    </div>
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                                            <td align="right">686</td>
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    <div class="container">
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                                        </tr>
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      <p class="lead">Biofilms are communities of bacteria that live together on a surface, surrounded by a protective layer, like dental plaque. Biofilms consist up to 85% of nanowires. These nanowires are composed of CsgA proteins. All the parts in this collection contain the CsgA gene controlled by a rhamnose inducible promoter. However, most have an additional function, like the His-tag for purification, or the hydroxyapatite tag, that gives the CsgA proteins better adhesive properties to teeth, thereby closer resembling dental plaque. Additionally, several biobricks contain RFP or GFP genes, to improve detection.
+
                                        <tr>
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      </p>
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                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890001">BBa_K1890001</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>Silicatein gene, fused to transmembrane domain of INP, with strong RBS</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">1442</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890002">BBa_K1890002</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>Silicatein gene, fused to transmembrane domain of OmpA, with strong RBS</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">1481</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890010">BBa_K1890010</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>mCerulean with strong consitutive promoter and RBS</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">784</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890011">BBa_K1890011</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>mVenus with strong consitutive promoter and RBS</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">784</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890020">BBa_K1890020</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with strong constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890021">BBa_K1890021</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with strong constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890022">BBa_K1890022</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with medium constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890023">BBa_K1890023</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with weak constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890024">BBa_K1890024</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with weak constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890030">BBa_K1890030</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>BolA gene with RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">508</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890031">BBa_K1890031</a></td>
 +
                                            <td>Composite</td>
 +
                                            <td>BolA gene with strong promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">519</td>
 +
                                        </tr>
 +
                                    </tbody>
 +
                                </table>
  
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                    </div>
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1583100" target="_blank"><p class="lead">BBa_K1583100</p></a>
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<div class="row">
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                        <span class="anchor" id="basic"></span>
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<div class="col-md-9 col-xs-9">
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                        <h2 class="title-style-1">Basic Parts<span class="title-under"></span></h2>
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<p class="lead">pRha + CsgA</p>
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                        <p>The different basic parts comprising the synthesized parts we used are documented here.
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</div>
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                            Please note that these parts do not exist individually.
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</div>
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</p>
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                        <div class="col-md-10 col-md-offset-1"
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                            <table>
  
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                                <table class="table table-style-1 pgrouptable tablesorter">
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1583106" target="_blank"><p class="lead">BBa_K1583106</p></a>
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                                        <tr>
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</div>
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                                            <th>Name</th>
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<div class="col-md-9 col-xs-9">
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                                            <th>Type</th>
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<p class="lead">pRha + CsgA & pTet+RFP</p>
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                                            <th>Description</th>
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</div>
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                                            <th>Designer</th>
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</div>
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                                            <th>Length</th>
 +
                                        </tr>
  
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<div class="row" style="margin-bottom:5px">
+
                                    </thead>
−
<div class="col-md-3 col-xs-3">
+
                                    <tbody>
−
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1583101" target="_blank"><p class="lead">BBa_K1583101</p></a>
+
                                        <tr>
−
</div>
+
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890050">BBa_K1890050</a></td>
−
<div class="col-md-9 col-xs-9">
+
                                            <td>Coding</td>
−
<p class="lead">pRha + CsgA+His tag</p>
+
                                            <td>Silicatein from Suberites domuncula</td>
−
</div>
+
                                            <td>Lycka Kamoen, Maria Vazquez</td>
−
</div>
+
                                            <td align="right">660</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890051">BBa_K1890051</a></td>
 +
                                            <td>Coding</td>
 +
                                            <td>Silicatein from Tethya aurantium</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">993</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890052">BBa_K1890052</a></td>
 +
                                            <td>Protein_Domain</td>
 +
                                            <td>OmpA transmembrane domain</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">462</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890053">BBa_K1890053</a></td>
 +
                                            <td>Protein_Domain</td>
 +
                                            <td>INP transmembrane domain</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">732</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890054">BBa_K1890054</a></td>
 +
                                            <td>Coding</td>
 +
                                            <td>mCerulean</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">720</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890055">BBa_K1890055</a></td>
 +
                                            <td>Coding</td>
 +
                                            <td>mVenus</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">720</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890056">BBa_K1890056</a></td>
 +
                                            <td>Coding</td>
 +
                                            <td>BolA</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">321</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890058">BBa_K1890058</a></td>
 +
                                            <td>DNA</td>
 +
                                            <td>Linker</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">24</td>
 +
                                        </tr>
 +
                                    </tbody>
 +
                                </table>
  
−
<div class="row" style="margin-bottom:5px">
+
                        </div>
−
<div class="col-md-3 col-xs-3">
+
                    </div>
−
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1583107" target="_blank"><p class="lead">BBa_K1583107</p></a>
+
                    <div class="row">
−
</div>
+
                        <span class="anchor" id="collection"></span>
−
<div class="col-md-9 col-xs-9">
+
                        <h2 class="title-style-1">Part Collection<span class="title-under"></span></h2>
−
<p class="lead">pRha + CsgA+His tag & pTet+RFP</p>
+
                        <p>We provided a small collection of GFP expression devices with different promoter
−
</div>
+
                        strengths, which we used to study the effect of different levels of constitutive expression. These parts are
−
</div>
+
                        ready-to-go expression units for green fluorescence, which can be useful for future teams.
 +
By constructing these parts, we improved both the function and the characterization of the <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_E0840" target="_blank">original part BBa_E0840</a></b>.</p>
 +
                    </div>
 +
                    <div class="col-md-10 col-md-offset-1"
 +
                           
 +
                            <table>
  
−
<div class="row" style="margin-bottom:5px">
+
                                <table class="table table-style-1 pgrouptable tablesorter">
−
<div class="col-md-3 col-xs-3">
+
                                    <thead>
−
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1583102" target="_blank"><p class="lead">BBa_K1583102</p></a>
+
                                        <tr>
−
</div>
+
                                            <th>Name</th>
−
<div class="col-md-9 col-xs-9">
+
                                            <th>Type</th>
−
<p class="lead">pRha + CsgA+Hydroxyapatite affinity tag</p>
+
                                            <th>Description</th>
−
</div>
+
                                            <th>Designer</th>
−
</div>
+
                                            <th>Length</th>
 +
                                        </tr>
 +
                                    </thead>
 +
                                    <tbody>
 +
                                       
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890020">BBa_K1890020</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with strong constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890021">BBa_K1890021</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with strong constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890022">BBa_K1890022</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with medium constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890023">BBa_K1890023</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with weak constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1890024">BBa_K1890024</a></td>
 +
                                            <td>Generator</td>
 +
                                            <td>GFP with weak constitutive promoter, RBS and terminator</td>
 +
                                            <td>Lycka Kamoen, Maria Vazquez</td>
 +
                                            <td align="right">913</td>
 +
                                        </tr>
 +
                                    </tbody>
 +
                                </table>
  
−
<div class="row" style="margin-bottom:5px">
+
                        </div>
−
<div class="col-md-3 col-xs-3">
+
−
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1583108" target="_blank"><p class="lead">BBa_K1583108</p></a>
+
−
</div>
+
−
<div class="col-md-9 col-xs-9">
+
−
<p class="lead">pRha + CsgA with Hydroxyapatite affinity tag & pTet RFP</p>
+
−
</div>
+
−
</div>
+
  
−
<div class="row" style="margin-bottom:5px">
+
                </div>
−
<div class="col-md-3 col-xs-3">
+
            </div>
−
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1583105" target="_blank"><p class="lead">BBa_K1583105</p></a>
+
        </div>
−
</div>
+
        <!-- Scripts================================================== -->
−
<div class="col-md-9 col-xs-9">
+
−
<p class="lead">pRha + fusion protein of CsgA with Mfp3 + His tag</p>
+
−
</div>
+
−
</div>
+
  
−
<div class="row" style="margin-bottom:5px">
+
        <script type="text/javascript" src="https://2016.igem.org/Template:TU_Delft/JS?action=raw&ctype=text/javascript"></script>
−
<div class="col-md-3 col-xs-3">
+
        <!-- Bootsrap javascript file -->
−
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1583111" target="_blank"><p class="lead">BBa_K1583111</p></a>
+
        <script type="text/javascript" src="https://2016.igem.org/Template:TU_Delft/BootstrapJS?action=raw&ctype=text/javascript"></script>
−
</div>
+
−
<div class="col-md-9 col-xs-9">
+
−
<p class="lead">pRha + fusion protein of CsgA with Mfp3 + His tag & pTET + RFP</p>
+
−
</div>
+
−
</div>
+
  
−
<div class="row" style="margin-bottom:5px">
+
<script type="text/javascript" src ="https://igem.org/common/tablesorter/jquery.tablesorter.min.js"></script>
−
<div class="col-md-3 col-xs-3">
+
        <link rel="stylesheet" type="text/css" href="https://igem.org/common/tablesorter/themes/groupparts/style.css"/>
−
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1583104" target="_blank"><p class="lead">BBa_K1583104</p></a>
+
        <link rel="stylesheet" type="text/css" href="https://igem.org/common/table_styles.css"/>
−
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+
<script type="text/javascript" src ="https://igem.org/wiki/skins/Igem/resources/2016_skin.js"></script>
−
<div class="col-md-9 col-xs-9">
+
−
<p class="lead">pRha + fusion protein of Mfp5 with CsgA + His tag</p>
+
−
</div>
+
−
</div>
+
  
 +
    </body>
 +
</html>
  
−
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−
<div class="col-md-3 col-xs-3">
 
−
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1583110" target="_blank"><p class="lead">BBa_K1583110</p></a>
 
−
</div>
 
−
<div class="col-md-9 col-xs-9">
 
−
<p class="lead">pRha + fusion protein of Mfp5 with CsgA + His tag & pTET + RFP</p>
 
−
</div>
 
−
</div>
 
−
 
−
<div class="row" style="margin-bottom:5px">
 
−
<div class="col-md-3 col-xs-3">
 
−
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1583112" target="_blank"><p class="lead">BBa_K1583112</p></a>
 
−
</div>
 
−
<div class="col-md-9 col-xs-9">
 
−
<p class="lead">pRha + CsgA & GFP in same operon</p>
 
−
</div>
 
−
</div>
 
−
    </div>
 
−
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−
 
−
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{{:Team:TU_Delft/Footer}}
 
{{:Team:TU_Delft/Footer}}

Latest revision as of 20:27, 19 October 2016

iGEM TU Delft

Parts

For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts were designed containing the coding sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are composite. The different basic parts comprising the composite parts are documented seperately, although they do not exist induvidually. In the case of a constitutive expression, the promoter is included in the biobrick itself. For the inducible expression, however, we made use of a plasmid backbone containing a Lac-promoter and the LacI gene, which encodes for the Lac repressor. Consequently, these parts themselves do not contain a promoter. Additionally, using PCR rather than synthesis, we constructed a small collection of GFP expression devices with different promoter strengths, based on an existing biobrick. Thereby improving both the function and characterization of the original part

                               <groupparts class="parts">iGEM2016 TU_Delft</groupparts>

Composite Parts

For the construction of our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts were designed containing the coding sequence, RBS, promoter and terminators, if applicable. Therefore, all of the parts we used are composite.

Name Type Description Designer Length
BBa_K1890000 Composite Silicatein gene with strong RBS Lycka Kamoen, Maria Vazquez 686
BBa_K1890001 Composite Silicatein gene, fused to transmembrane domain of INP, with strong RBS Lycka Kamoen, Maria Vazquez 1442
BBa_K1890002 Composite Silicatein gene, fused to transmembrane domain of OmpA, with strong RBS Lycka Kamoen, Maria Vazquez 1481
BBa_K1890010 Composite mCerulean with strong consitutive promoter and RBS Lycka Kamoen, Maria Vazquez 784
BBa_K1890011 Composite mVenus with strong consitutive promoter and RBS Lycka Kamoen, Maria Vazquez 784
BBa_K1890020 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890021 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890022 Generator GFP with medium constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890023 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890024 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890030 Composite BolA gene with RBS and terminator Lycka Kamoen, Maria Vazquez 508
BBa_K1890031 Composite BolA gene with strong promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 519

Basic Parts

The different basic parts comprising the synthesized parts we used are documented here. Please note that these parts do not exist individually.

Name Type Description Designer Length
BBa_K1890050 Coding Silicatein from Suberites domuncula Lycka Kamoen, Maria Vazquez 660
BBa_K1890051 Coding Silicatein from Tethya aurantium Lycka Kamoen, Maria Vazquez 993
BBa_K1890052 Protein_Domain OmpA transmembrane domain Lycka Kamoen, Maria Vazquez 462
BBa_K1890053 Protein_Domain INP transmembrane domain Lycka Kamoen, Maria Vazquez 732
BBa_K1890054 Coding mCerulean Lycka Kamoen, Maria Vazquez 720
BBa_K1890055 Coding mVenus Lycka Kamoen, Maria Vazquez 720
BBa_K1890056 Coding BolA Lycka Kamoen, Maria Vazquez 321
BBa_K1890058 DNA Linker Lycka Kamoen, Maria Vazquez 24

Part Collection

We provided a small collection of GFP expression devices with different promoter strengths, which we used to study the effect of different levels of constitutive expression. These parts are ready-to-go expression units for green fluorescence, which can be useful for future teams. By constructing these parts, we improved both the function and the characterization of the original part BBa_E0840.

Name Type Description Designer Length
BBa_K1890020 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890021 Generator GFP with strong constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890022 Generator GFP with medium constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890023 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913
BBa_K1890024 Generator GFP with weak constitutive promoter, RBS and terminator Lycka Kamoen, Maria Vazquez 913

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