Difference between revisions of "Team:FAFU-CHINA/Composite Part"
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| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cry4Aa4+Extended2A+Cyt1-pChlamy3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in JM108</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">4422bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles, and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td colspan="2" valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs13.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs16.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by BamHI and KpnI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><i><span style="font-size:9.0pt;font-family:":,sans-serif;color:#595959"> </span></i><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td width="63" colspan="2" valign="top" style="width:47.25pt;padding:0cm 0cm 0cm 0cm"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Tahoma, sans-serif"> </span></p> | ||
| + | </td> </tr> <tr> <td width="137" valign="top" style="width:102.75pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> <td width="39" valign="top" style="width:29.25pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> <td width="132" valign="top" style="width:99.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> <td width="51" valign="top" style="width:38.25pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> <td width="128" valign="top" style="width:96.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> <td width="7" valign="top" style="width:5.25pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> <td width="56" valign="top" style="width:42.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> </tr> | ||
| + | </table> | ||
| + | = <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:":,sans-serif;color:#1E4E79">Cry10Aa4+Extended2A+Cyt2-pChlamy3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> = | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cry10Aa4+Extended2A+Cyt2-pChlamy3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in DH5α</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2946bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles, and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs13.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs18.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><i><span style="font-size:9.0pt;font-family:":,sans-serif;color:#595959"> </span></i><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:":,sans-serif;color:#1E4E79"> </span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:":,sans-serif;color:#1E4E79"> </span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | = <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:":,sans-serif;color:#1E4E79">Cry10Aa4+Extended2A+Cyt2-pChlamy3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> = | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cry10Aa4+Extended2A+Cyt2-pChlamy3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in DH5α</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2946bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles, and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs20.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><i><span style="font-size:9.0pt;font-family:":,sans-serif;color:#595959"> </span></i><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | = <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:":,sans-serif;color:#1E4E79">Cry10Aa4+Extended2A+Cyt1-pChlamy3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> = | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cry10Aa4+Extended2A+Cyt1-pChlamy3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in DH5α</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2907bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="3" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles, and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td colspan="2" valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs22.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td width="60" colspan="2" valign="top" style="width:45.0pt;padding:0cm 0cm 0cm 0cm"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Tahoma, sans-serif"> </span></p> | ||
| + | </td> </tr> <tr> <td width="135" valign="top" style="width:101.25pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> <td width="45" valign="top" style="width:33.75pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> <td width="131" valign="top" style="width:98.25pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> <td width="78" valign="top" style="width:58.5pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> <td width="101" valign="top" style="width:75.75pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> <td width="5" valign="top" style="width:3.75pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> <td width="55" valign="top" style="width:41.25pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> </tr> | ||
| + | </table> | ||
| + | = <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:":,sans-serif;color:#1E4E79">Cry11Aa4+Extended2A+Cyt2-pChlamy3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> = | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cry11Aa4+Extended2A+Cyt2-pChlamy3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in Top10</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2859bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles, and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs24.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by </span><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | = <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:":,sans-serif;color:#1E4E79">Cry11Aa4+Extended2A+Cyt1-pChlamy3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> = | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cry11Aa4+Extended2A+Cyt1-pChlamy3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in JM108</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2820bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles, and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs26.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><i><span style="font-size:9.0pt;font-family:":,sans-serif;color:#595959"> </span></i><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | = <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:":,sans-serif;color:#1E4E79">Cry11Aa4+Extended2A+EGFP-pChlamy3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> = | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cry11Aa4+Extended2A+EGFP-pChlamy3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in JM108</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2790bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles, and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | |||
| + | <table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse"> | ||
| + | <tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体"> [[File:T--FAFU-CHINA--bs28.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><i><span style="font-size:9.0pt;font-family:":,sans-serif;color:#595959"> </span></i><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | </td> </tr> | ||
| + | </table> | ||
| + | <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif"> </span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> | ||
| + | <p><span> </span></p> | ||
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Revision as of 20:49, 19 October 2016
Contents
Cry4Aa4+Extended2A+Cyt1-pChlamy3
| Gene Name | Cry4Aa4+Extended2A+Cyt1-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in JM108 |
| Gene Length | 4422bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results | |||
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass | |||
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass | |||
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass | |||
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass | |||
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass | |||
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass | |||
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass | |||
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by BamHI and KpnI
Lane 2: Plasmid DNA
|
| |||
Cry10Aa4+Extended2A+Cyt2-pChlamy3
| Gene Name | Cry10Aa4+Extended2A+Cyt2-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in DH5α |
| Gene Length | 2946bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry10Aa4+Extended2A+Cyt2-pChlamy3
| Gene Name | Cry10Aa4+Extended2A+Cyt2-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in DH5α |
| Gene Length | 2946bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry10Aa4+Extended2A+Cyt1-pChlamy3
| Gene Name | Cry10Aa4+Extended2A+Cyt1-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in DH5α |
| Gene Length | 2907bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results | |||
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass | |||
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass | |||
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass | |||
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass | |||
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass | |||
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass | |||
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass | |||
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
| |||
Cry11Aa4+Extended2A+Cyt2-pChlamy3
| Gene Name | Cry11Aa4+Extended2A+Cyt2-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in Top10 |
| Gene Length | 2859bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
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Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry11Aa4+Extended2A+Cyt1-pChlamy3
| Gene Name | Cry11Aa4+Extended2A+Cyt1-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in JM108 |
| Gene Length | 2820bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry11Aa4+Extended2A+EGFP-pChlamy3
| Gene Name | Cry11Aa4+Extended2A+EGFP-pChlamy3 |
| Cloning Vector | pChlamy_3 |
| Construct Resistant | Ampicillin in JM108 |
| Gene Length | 2790bp |
| Cloning Strategy | XbaI and PstI |
| QC Items | Method | Specifications | Results |
| Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
| Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
| Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
| Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
| Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
| Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
| Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
