Difference between revisions of "Team:Bielefeld-CeBiTec/Experiments/Primers"

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Add primers here
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{{Template:Bielefeld-CeBiTec/header}}
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<html>
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<body>
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<div class="container main">
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<div class="jumbotron" style="background-image: url(https://static.igem.org/mediawiki/2016/a/ab/Bielefeld_CeBiTec_2016_10_14_X_notebook.png)">
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<div class="jumbotron-text">
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<h1 style="margin-bottom: 0px; text-align:left">Primers</h1>
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<h2 style="color:#ffffff; text-align:left">Primed for success</h2>
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</div>
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</div>
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<p style="position: relative; font-size: 9px; top: -50px; color: white; float: right; right: 10px;"></p>
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<div class="container text_header"><h3>Primers</h3></div>
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<br>
 +
<div class="container text"><button data-toggle="collapse" class="btn-block" data-target="#standard">Standard Primers</button >
 +
<div id="standard" class="collapse">
 +
<table class="table">
 +
    <thead>
 +
      <tr>
 +
        <th>Name</th>
 +
        <th>Sequence (5' &rarr; 3')</th>
 +
      </tr>
 +
    </thead>
 +
    <tbody>
 +
      <tr>
 +
        <td>VF</td>
 +
        <td>CCACCTGACGTCTAAGAAAC</td>
 +
      </tr>
 +
      <tr>
 +
        <td>VR</td>
 +
        <td>GTATTACCGCCTTTGAGTGAGC</td>
 +
      </tr>
 +
    </tbody>
 +
  </table>
 +
</div>
 +
<br><br>
 +
<button data-toggle="collapse" class="btn-block" data-target="#library">Library Primers</button>
 +
<div id="library" class="collapse">
 +
<table class="table">
 +
    <thead>
 +
      <tr>
 +
        <th>Name</th>
 +
        <th>Sequence (5' &rarr; 3')</th>
 +
      </tr>
 +
    </thead>
 +
    <tbody>
 +
      <tr>
 +
        <td>Target_bb_fw</td>
 +
        <td>TACTAGAGAAAAAAAAACCCCGCCCC</td>
 +
      </tr>
 +
      <tr>
 +
        <td>Target_bb_rev</td>
 +
        <td>CAAGTCCTCTTCAGAAATAAGCTTTTGTTC</td>
 +
      </tr>
 +
<tr>
 +
        <td>MB-Insert-fw</td>
 +
        <td>GAACAAAAGCTTATTTCTGAAGAGGACTTGGTTTCTTCTGTTCCGACCAAACTGG</td>
 +
      </tr>
 +
      <tr>
 +
        <td>MB-Insert-rev</td>
 +
        <td>GTCAGGGGCGGGGTTTTTTTTTCTCTAGTAGGTACGGTAGTTGATAGAGATCGGA</td>
 +
      </tr>
 +
<tr>
 +
        <td>NB-Insert-fw</td>
 +
        <td>GAACAAAAGCTTATTTCTGAAGAGGACTTGCAGGTTCAGCTGGTTGAATCTGGT</td>
 +
      </tr>
 +
      <tr>
 +
        <td>NB-Insert-rev</td>
 +
        <td>GGGCGGGGTTTTTTTTTCTCTAGTAAGCAGCGCAGTAGTAGATAGCGG</td>
 +
      </tr>
 +
<tr>
 +
        <td>NB-bb-fw</td>
 +
        <td>GGGTACGCAGGTTACCGTTTCTTCTTACTAGAGAAAAAAAAACCCCGCCCC</td>
 +
      </tr>
 +
      <tr>
 +
        <td>NB-bb-rev</td>
 +
        <td>AGCAGCGCAGTAGTAGATAGCGG</td>
 +
      </tr>
 +
<tr>
 +
        <td>MB-bb-fw</td>
 +
        <td>TCTCCGATCTCTATCAACTACCGTAC</td>
 +
      </tr>
 +
      <tr>
 +
        <td>MB-bb-rev</td>
 +
        <td>AACGGTAACAGCCGGAGCGTC</td>
 +
      </tr>
 +
<tr>
 +
        <td>NB-Control-fw</td>
 +
        <td>ACACCGTTACCCTGCAAATGAACAAC</td>
 +
      </tr>
 +
      <tr>
 +
        <td>NB-control-rev</td>
 +
        <td>ACTGCAGCGGCCGCTACTAGTA</td>
 +
      </tr>
 +
<tr>
 +
        <td>NB-psb-I1-fw</td>
 +
        <td>GGAATTCGCGGCCGCTTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACT</td>
 +
      </tr>
 +
      <tr>
 +
        <td>NB-psb-I1-rev</td>
 +
        <td>GTACCTAGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCAT</td>
 +
      </tr>
 +
<tr>
 +
        <td>NB-psb-I2-fw</td>
 +
        <td>CCCTGACAGGGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAGTCCGGCAAA</td>
 +
      </tr>
 +
      <tr>
 +
        <td>NB-psb-I2-rev</td>
 +
        <td>GACTGCAGCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCG</td>
 +
      </tr>
 +
<tr>
 +
        <td>MB-psb-1-fw</td>
 +
        <td>GGAATTCGCGGCCGCTTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTAC</td>
 +
      </tr>
 +
      <tr>
 +
        <td>MB-psb-1-rev</td>
 +
        <td>GTACCTAGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCT</td>
 +
      </tr>
 +
<tr>
 +
        <td>MB-psb-3-fw</td>
 +
        <td>CTACCCCGACCTCTCTGCTGATCTCTTGGGACGCTCCGGCTGTTACCGTT</td>
 +
      </tr>
 +
      <tr>
 +
        <td>MB-psb-3-rev</td>
 +
        <td>GTAACAGCCGGAGCGTCCCAAGAGATCAGCAGAGAGGTCGGGGTAGCAGCA</td>
 +
      </tr>
 +
<tr>
 +
        <td>MB-psb-4-fw</td>
 +
        <td>CTCCGATCTCTATCAACTACCGTACCAAAAAAAAACCCCGCCCCTGACAGGGCGGG</td>
 +
      </tr>
 +
      <tr>
 +
        <td>MB-psb-4-rev</td>
 +
        <td>CCTGTCAGGGGCGGGGTTTTTTTTTGGTACGGTAGTTGATAGAGATCGGAGA</td>
 +
      </tr>
 +
<tr>
 +
        <td>BIFC_GFP_contr_fw</td>
 +
        <td>ATGTCTAAAGGTGAAGAACTGTTCACCG</td>
 +
      </tr>
 +
      <tr>
 +
        <td>BIFC_GFP_contr_rev</td>
 +
        <td>TCCGGAACGTCGTACGGGTACAT</td>
 +
      </tr>
 +
<tr>
 +
        <td>BIFC_BINDER_contr_fw</td>
 +
        <td>GTTCAGCTGGTTGAATCTGGTGGT</td>
 +
      </tr>
 +
      <tr>
 +
        <td>BIFC_BINDER_contr_rev</td>
 +
        <td>TTCGATGTTGTGACGGATTTTGAAGTTAGC</td>
 +
      </tr>
 +
<tr>
 +
        <td>LIB-bb-fw</td>
 +
        <td>CTGGAATTCGCGGCCGCTTCTAGAG</td>
 +
      </tr>
 +
      <tr>
 +
        <td>LIB-bb-rev</td>
 +
        <td>CTCTAGAAGCGGCCGCGAATTCCAG</td>
 +
      </tr>
 +
<tr>
 +
        <td>Phag_MB_pAK100blaKDIR_fw</td>
 +
        <td>CGCTGGATTGTTATTACTCGCGGCCCAGCCGGCCTTTGCTGTTTCTTCTGTTCCGACCAAACTGGAA</td>
 +
      </tr>
 +
      <tr>
 +
        <td>Phag_MB_pAK100_rev</td>
 +
        <td>CTTCTGCTCGAATTCGGCCCCCGAGGCCGAGTAGGTACGGTAGTTGATAGAGATCG</td>
 +
      </tr>
 +
<tr>
 +
        <td>Phag_NB_pAK100blaKDIR _fw</td>
 +
    <td>CGCTGGATTGTTATTACTCGCGGCCCAGCCGGCCTTTGCTCAGGTTCAGCTGGTTGAATCTGGT</td>
 +
      </tr>
 +
      <tr>
 +
        <td>Phag_MB_pAK100_rev</td>
 +
        <td>CTTCTGCTCGAATTCGGCCCCCGAGGCCGAGTAAGAAGAAACGGTAACCTGCGTAC</td>
 +
      </tr>
 +
<tr>
 +
        <td>Zk_Seq_fw1</td>
 +
        <td>CGTGGTGTGAAGCTGAACAAAAGCTT</td>
 +
      </tr>
 +
      <tr>
 +
        <td>Zk_Seq_rev1</td>
 +
        <td>AATCCCACGCGGTATCGCCCA</td>
 +
      </tr>
 +
<tr>
 +
        <td>Zk_Seq_fw2</td>
 +
        <td>TGCATGGCAGCCAGCATAGCG</td>
 +
      </tr>
 +
      <tr>
 +
        <td>Zk_Seq_rev2</td>
 +
        <td>CATTTCCGCTTCCAGCGCGC</td>
 +
      </tr>
 +
<tr>
 +
        <td>Zk_Insert_pSB1C3_fw</td>
 +
    <td>GATTTCTGGAATTCGCGGCCGCTTCTAGAGATTAGATGTATCGGCGTAAGTAATCGCG</td>
 +
      </tr>
 +
      <tr>
 +
        <td>Zk_Insert_pSB1C3_rev</td>
 +
        <td>TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAGGCAGAGACCGCAGTTGACAAAAA</td>
 +
      </tr>
 +
<tr>
 +
        <td>Zk_cMyc_BB_fw</td>
 +
        <td>ATCTTTTTGTCAACTGCGGTCTCTGCCTAATACTAGAGAAAAAAAAACCCCGCCCCT</td>
 +
      </tr>
 +
      <tr>
 +
        <td>Zk_cMyc_BB_rev</td>    <td>GTCGCGATTACTTACGCCGATACATCTAATCAAGTCCTCTTCAGAAATAAGCTTTTGTTCA</td>
 +
      </tr>
 +
    </tbody>
 +
  </table>
 +
</div>
 +
<br><br>
 +
<button data-toggle="collapse" class="btn-block" data-target="#mutation">Mutation Primers</button>
 +
<div id="mutation" class="collapse">
 +
<table class="table">
 +
    <thead>
 +
      <tr>
 +
        <th>Name</th>
 +
        <th>Sequence (5' &rarr; 3')</th>
 +
      </tr>
 +
    </thead>
 +
    <tbody>
 +
    <tr>
 +
<td> emrR_Gib_fw</td>
 +
<td>GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCACACAGGAAACCTACTAGATGGATAGTTCGTTTACGCCC</td>
 +
</tr>
 +
<tr>
 +
<td> emrR_Gib_rev</td>
 +
<td>TAAATTTGTCATCTAGTAGGTTTCCTGTGTGATTATTAGCTCATCGCTTCGAGAAC</td>
 +
</tr>
 +
<tr>
 +
<td> cPCR_ugi_cda1</td>
 +
<td>CTGGGGTTACGCGGTTAAC</td>
 +
</tr>
 +
<tr>
 +
<td> ugi_Gib_fw</td>
 +
<td>GCGATGAGCTAATAATCACACAGGAAACCTACTAGATGACAAATTTATCTGACATCATTGA</td>
 +
</tr>
 +
<tr>
 +
<td> ugi_Gib_rev</td>
 +
<td>CGCGTCGGTCATCTAGTAGGTTTCCTGTGTGATTATTATAACATTTTAATTTTATTTTCTCCATTACT</td>
 +
</tr>
 +
<tr>
 +
<td>ugi-cda1_Gib_fw</td>
 +
<td>AAAATGTTATAATAATCACACAGGAAACCTACTAGATGACCGACGCGGAATAC</td>
 +
</tr>
 +
<tr>
 +
<td>ugi-cda1_Gib_rev</td>
 +
<td>GACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAAACCGCCGGAGATTTGG</td>
 +
</tr>
 +
<tr>
 +
<td>WT_PolI_Gib_for</td>
 +
<td>ACCATGATTACGCCAAGCTTATGGTTCAGATCCCCCAAAA</td>
 +
</tr>
 +
<tr>
 +
<td>WT_PolI_Gib_rev</td>
 +
<td>AACGACGGCCAGTGGAATTCTTAGTGCGCCTGATCCCAGT</td>
 +
</tr>
 +
<tr>
 +
<td>pEPPolI_Gib_for</td>
 +
<td>GAATTCCACTGGCCGTCGTT</td>
 +
</tr>
 +
<tr>
 +
<td>pEPPolI_Gib_rev</td>
 +
<td>TTTTGGGGGATCTGAACCATAAGC</td>
 +
</tr>
 +
<tr>
 +
<td>pEPPolI_Seq_for</td>
 +
<td>GCACCCCAGGCTTTACAC</td>
 +
</tr>
 +
<tr>
 +
<td>pEPPolI_Seq_rev</td>
 +
<td>GGGTAACGCCAGGGTTTT</td>
 +
</tr>
 +
<tr>
 +
<td>pEPPolI_Col_rev</td>
 +
<td>GCGGGCGATGTGATTTGT</td>
 +
</tr>
 +
<tr>
 +
<td>pSB1K3_Del_for</td>
 +
<td>GCTGAGTTGAAGGATCAGCTCAGGAAAGAACATGTGAGCA</td>
 +
</tr>
 +
<tr>
 +
<td>pSB1K3_Del_rev</td>
 +
<td>TGCTCACATGTTCTTTCCTGAGCTGATCCTTCAACTCAGC</td>
 +
</tr>
 +
<tr>
 +
<td>Sbla_T1_Gib_for</td>
 +
<td>GGTTCCTGGCCTTTTGCTGGCCTATTTGTTTATTTTTCTA</td>
 +
</tr>
 +
<tr>
 +
<td>Sbla_T1_Gib_rev</td>
 +
<td>ATCTTCAGCATCTTTTACTTACACCAGCGTTTCTGGGTGA</td>
 +
</tr>
 +
<tr>
 +
<td>Sbla_T2_Gib_for</td>
 +
<td>TCACCCAGAAACGCTGGTGTAAGTAAAAGATGCTGAAGAT</td>
 +
</tr>
 +
<tr>
 +
<td>Sbla_T2_Gib_rev</td>
 +
<td>AAGAACATGTGAGCAAAAGGATATGAGTAAACTTGGTCTG</td>
 +
</tr>
 +
<tr>
 +
<td>pSB1K3D_Gib_for</td>
 +
<td>CAGACCAAGTTTACTCATATCCTTTTGCTCACATGTTCTT</td>
 +
</tr>
 +
<tr>
 +
<td> pSB1K3D_Gib_rev </td>
 +
<td> TAGAAAAATAAACAAATAGGCCAGCAAAAGGCCAGGAACC </td>
 +
</tr>
 +
<tr>
 +
<td> Sbla_EndSeq_rev </td>
 +
<td> GCCTGGAGCAAGACGTTT </td>
 +
</tr>
 +
<tr>
 +
<td> pSB1K3D_Col_for </td>
 +
<td> ACACAACGTGGCTTTGTTG </td>
 +
</tr>
 +
<tr>
 +
<td> pSB1K3D_Col_rev </td>
 +
<td> GGTTCCTGGCCTTTTGCT </td>
 +
</tr>
 +
<tr>
 +
<td> Sbla_Col_for </td>
 +
<td> GGTTTCGCCACCTCTGAC </td>
 +
</tr>
 +
<tr>
 +
<td> pSB1K3_D_for_P2 </td>
 +
<td> TCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTC </td>
 +
</tr>
 +
<tr>
 +
<td> pSB1K3_D_rev_P1 </td>
 +
<td> GGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGA </td>
 +
</tr>
 +
<tr>
 +
<td> RBS_RFP_Ter_for </td>
 +
<td> TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG </td>
 +
</tr>
 +
<tr>
 +
<td> RBS_RFP_Ter_rev </td>
 +
<td>CTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTATTAAGCACCGGTGGAGTGA </td>
 +
</tr>
 +
<tr>
 +
<td> C3BB_Ter_for </td>
 +
<td>TAGAGAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAGTCCG </td>
 +
</tr>
 +
<tr>
 +
<td> C3BB_And_01_rev </td>
 +
<td> CTCTTTCTCTAGTAGCTAGCATTGTACCTAGGACTGAGCTAGCCATAAACTCTAGAAGCGGCCGCGAATTCCAGAAATCA </td>
 +
</tr>
 +
<tr>
 +
<td> C3BB_And_047_rev </td>
 +
<td> CTCTTTCTCTAGTAGCTAGCACTATACCTAGGACTGAGCTAGCCGTAAACTCTAGAAGCGGCCGCGAATTCCAGAAATCA </td>
 +
</tr>
 +
<tr>
 +
<td> C3BB_And_086_rev </td>
 +
<td>CTCTTTCTCTAGTAGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCA</td>
 +
</tr>
 +
<tr>
 +
<td> VF2</td>
 +
<td> TGCCACCTGACGTCTAAGAA </td>
 +
</tr>
 +
<tr>
 +
<td> VR </td>
 +
<td> ATTACCGCCTTTGAGTGAGC </td>
 +
</tr>
 +
<tr>
 +
<td> BFP_Gib_for </td>
 +
<td> ACATACTAGAGAAAGAGGAGAAATACTAGATGAGCGAACTGATCAAAGAGAA </td>
 +
</tr>
 +
<tr>
 +
<td> BFP_Gib_rev </td>
 +
<td> GATCTACACTAGCACTATCAGCGTTATTAATTCAGTTTATGACCCAGCTTG </td>
 +
</tr>
 +
<tr>
 +
<td> K3_BFP_Gib_for </td>
 +
<td> CAAGCTGGGTCATAAACTGAATTAATAACGCTGATAGTGCTAGTGTAGATC </td>
 +
</tr>
 +
<tr>
 +
<td> K3_BFP_Gib_rev </td>
 +
<td> TTCTCTTTGATCAGTTCGCTCATCTAGTATTTCTCCTCTTTCTCTAGTATGT </td>
 +
</tr>
 +
<tr>
 +
<td> Web_C3BB_for </td>
 +
<td> GCCGCTGCAGTCCGGCAAAAAAG</td>
 +
</tr>
 +
<tr>
 +
<td> Web_C3BB_rev </td>
 +
<td> GCCGCGAATTCCAGAAATCATCCTTAGCG </td>
 +
</tr>
 +
<tr>
 +
<td> pSB1K3_D_rev_P2 </td>
 +
<td> TGCTCACATGTTCTTTCCTGCTCTGATGTTACATTGCACAAGATA </td>
 +
</tr>
 +
<tr>
 +
<td> cPCR_dnaQ_for </td>
 +
<td> GGTCACCGATAGCCTTGC </td>
 +
</tr>
 +
<tr>
 +
<td> cPCR_ugi-cda1_for </td>
 +
<td> CTGGGGTTACGCGGTTAAC </td>
 +
</tr>
 +
<tr>
 +
<td> dnaQ_Gib_for </td>
 +
<td> GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCACACAGGAAACCTACTAGATGAGCACTGCAATTACACGC </td>
 +
</tr>
 +
<tr>
 +
<td> dnaQ_Gib_rev </td>
 +
<td>ATTTTTCTTCATCTAGTAGGTTTCCTGTGTGATTATTATGCTCGCCAGAGGCAAC</td>
 +
</tr>
 +
<tr>
 +
<td>dam_Gib_for </td>
 +
<td>TGGCGAGCATAATAATCACACAGGAAACCTACTAGATGAAGAAAAATCGCGCTTTTTTG </td>
 +
</tr>
 +
<tr>
 +
<td> dam_Gib_rev </td>
 +
<td>TGGCGAGCATAATAATCACACAGGAAACCTACTAGATGAAGAAAAATCGCGCTTTTTTG </td>
 +
</tr>
 +
<tr>
 +
<td> seqA_Gib_for </td>
 +
<td>GCGAAAAAATAATAATCACACAGGAAACCTACTAGATGAAAACGATTGAAGTTGATGATG </td>
 +
</tr>
 +
<tr>
 +
<td> seqA_Gib_rev </td>
 +
<td>TGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAGATAGTTCCGCAAACCTTCTC </td>
 +
</tr>
 +
<tr>
 +
<td> cPCR_dam_for </td>
 +
<td> GGAAGCAGAGTTGTATCACTTCG </td>
 +
</tr>
 +
<tr>
 +
<td> PolI_Gib_for </td>
 +
<td> GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGTTCAGATCCCCCAAAATC </td>
 +
</tr>
 +
<tr>
 +
<td> PolI_Gib_rev </td>
 +
<td> TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAGTGCGCCTGATCCCAG </td>
 +
</tr>
 +
<tr>
 +
<td> E0040_A68T_Gib_for </td>
 +
<td> GGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAG </td>
 +
</tr>
 +
<tr>
 +
<td> E0040_A68T_Gib_rev </td>
 +
<td> GTGAAAAGTTCTTCTCCTTTACGCATCTAGTATTTCTCCTCTTTC </td>
 +
</tr>
 +
<tr>
 +
<td> E0040_C379A_Gib_for </td>
 +
<td> TCAAAGATGACGGGAACTAAAAGACACGTGCTGAAGTCAA </td>
 +
</tr>
 +
<tr>
 +
<td> E0040_C379A_Gib_rev </td>
 +
<td> TGACTTCAGCACGTGTCTTTTAGTTCCCGTCATCTTTGAAAAATATAG </td>
 +
</tr>
 +
<tr>
 +
<td> E0040_A380T_Gib_for </td>
 +
<td> CAAAGATGACGGGAACTACTAGACACGTGCTGAAGTCAAG </td>
 +
</tr>
 +
<tr>
 +
<td> E0040_A380T_Gib_rev </td>
 +
<td> TTGACTTCAGCACGTGTCTAGTAGTTCCCGTCATCTTTGAAAAAT </td>
 +
</tr>
 +
<tr>
 +
<td> GFP_MutSeq_for </td>
 +
<td> GATGCAACATACGGAAAACTTACC </td>
 +
</tr>
 +
<tr>
 +
<td> Pbad_Change_for </td>
 +
<td> CCGTCCACCCCCTAATATCCCGACATACCCGTTTTTTGGGCTAACT </td>
 +
</tr>
 +
<tr>
 +
<td> Pbad_Change_rev </td>
 +
<td> GATATTAGGGGGTGGACGGGGGGGGCGTCAGGTAGGATCCGC </td>
 +
</tr>
 +
<tr>
 +
<td> Pbad_Conf_rev </td>
 +
<td> GGATATTAGGGGGTGGACGG </td>
 +
</tr>
 +
<tr>
 +
<td> dnaQ_Exp_for </td>
 +
<td> TCACACAGGAAACCTACTAGATGAGCACTGCAATTACACGC </td>
 +
</tr>
 +
<tr>
 +
<td> dnaQ_Exp_rev </td>
 +
<td> CTACACTAGCACTATCAGCGTTATGCTCGCCAGAGGCAAC </td>
 +
</tr>
 +
<tr>
 +
<td> dnaQ_ExpBB_for </td>
 +
<td> GTTGCCTCTGGCGAGCATAACGCTGATAGTGCTAGTGTAGATC </td>
 +
</tr>
 +
<tr>
 +
<td> dnaQ_ExpBB_rev </td>
 +
<td> CGTGTAATTGCAGTGCTCATCTAGTAGGTTTCCTGTGTGACTC </td>
 +
</tr>
 +
<tr>
 +
<td> PolI_Exp_for </td>
 +
<td> TCACACAGGAAACCTACTAGATGGTTCAGATCCCCCAAAATC </td>
 +
</tr>
 +
<tr>
 +
<td> PolI_Exp_rev </td>
 +
<td> CTACACTAGCACTATCAGCGTTAGTGCGCCTGATCCCA </td>
 +
</tr>
 +
<tr>
 +
<td> PolI_Exp_rev </td>
 +
<td> ACTGGGATCAGGCGCACTAACGCTGATAGTGCTAGTGTAGATC </td>
 +
</tr>
 +
<tr>
 +
<td> PolI_ExpBB_rev </td>
 +
<td> TTTTGGGGGATCTGAACCATCTAGTAGGTTTCCTGTGTGACTC </td>
 +
</tr>
 +
<tr>
 +
<td> PolI_Seq_A </td>
 +
<td> ACGCTTATCAATACCATGACG </td>
 +
</tr>
 +
<tr>
 +
<td> PolI_Seq_B </td>
 +
<td> TGACAACTACGTCACCATCC </td>
 +
</tr>
 +
<tr>
 +
<td> PolI_Seq_C </td>
 +
<td> CGTTCGAAGTAAAGGTCCATG </td>
 +
</tr>
 +
<tr>
 +
<td> SGFP_A_for </td>
 +
<td> GGAGAAATACTAGATGCGTTAAGGAGAAGAACTTTTCACTGGAG </td>
 +
</tr>
 +
<tr>
 +
<td> SGFP_A_rev </td>
 +
<td> GTGAAAAGTTCTTCTCCTTAACGCATCTAGTATTTCTCCTCTTTC </td>
 +
</tr>
 +
<tr>
 +
<td> ugi_Single_for </td>
 +
<td> TAAAATTAAAATGTTATAATACTAGTAGCGGCCGCT </td>
 +
</tr>
 +
<tr>
 +
<td> ugi_Single_rev </td>
 +
<td> CGCTACTAGTATTATAACATTTTAATTTTATTTTCTCCATTACTGTC </td>
 +
</tr>
 +
<tr>
 +
<td> cda1_Single_for </td>
 +
<td> CTTCTAGAGATGACCGACGCGGAATACG </td>
 +
</tr>
 +
<tr>
 +
<td> cda1_Single_rev </td>
 +
<td> GCGTCGGTCATCTCTAGAAGCGGCCGC </td>
 +
</tr>
 +
<tr>
 +
<td> dam_Single_for </td>
 +
<td> CCGCGAAAAAATAATACTAGTAGCGGCCGCT </td>
 +
</tr>
 +
<tr>
 +
<td> dam_Single_rev </td>
 +
<td> CTACTAGTATTATTTTTTCGCGGGTGAAACG </td>
 +
</tr>
 +
<tr>
 +
<td> seqA_Single_for </td>
 +
<td> GCTTCTAGAGATGAAAACGATTGAAGTTGATGATGAACT </td>
 +
</tr>
 +
<tr>
 +
<td> SeqA_Single_for </td>
 +
<td> CAATCGTTTTCATCTCTAGAAGCGGCCGC </td>
 +
</tr>
 +
<tr>
 +
<td> MP6_ExpBB_for </td>
 +
<td> CCAAATCTCCGGCGGTTTAACGCTGATAGTGCTAGTGTAGATC </td>
 +
</tr>
 +
<tr>
 +
<td> MP6_Exp_rev </td>
 +
<td> CTACACTAGCACTATCAGCGTTAAACCGCCGGAGATTTGGT </td>
 +
</tr>
 +
<tr>
 +
<td> B0031_Conf_for </td>
 +
<td> TCACACAGGAAACCTACTAGATG </td>
 +
</tr>
 +
<tr>
 +
<td> AraProm_VF </td>
 +
<td> CGGGACCAAAGCCATGAC </td>
 +
</tr>
 +
<tr>
 +
<td> VF2</td>
 +
<td> TGCCACCTGACGTCTAAGAA </td>
 +
</tr>
 +
<tr>
 +
<td> VR </td>
 +
<td> ATTACCGCCTTTGAGTGAGC </td>
 +
</tr>
 +
<tr>
 +
<td> araE_Ins_for </td>
 +
<td> AGAAAGAGGAGAAATACTAGATGGTTACTATCAATACGGAATCTGC </td>
 +
</tr>
 +
<tr>
 +
<td> araE_Ins_rev </td>
 +
<td> GGGTTTTTTTTTCTCTAGTATCAGACGCCGATATTTCTCAAC </td>
 +
</tr>
 +
<tr>
 +
<td> araE_BB_for </td>
 +
<td> TGAGAAATATCGGCGTCTGATACTAGAGAAAAAAAAACCCCGCC </td>
 +
</tr>
 +
<tr>
 +
<td> araE_BB_rev </td>
 +
<td> TCCGTATTGATAGTAACCATCTAGTATTTCTCCTCTTTCTCTAGTAGC </td>
 +
</tr>
 +
<tr>
 +
  <td>Bb_site_fw</td>
 +
  <td>CGCTTCTAGA
 +
GTACTAGTAGCGGCCGCTGCAGGGGTGCTACTTAAGCCTTTAGG</td>
 +
</tr>
 +
<tr>
 +
  <td>Bb_site_rev</td>
 +
  <td >CTACTAGTACTCTAGAAGCGGCCGCGAATTCTCGCAAGCTCGGGCAAATCG</td>
 +
</tr>
 +
<tr>
 +
  <td>Spec_fw</td>
 +
  <td>ACCAATAACTGCCTTAAAAAAACTAATTGAGAGAAGTTTCTATA</td>
 +
</tr>
 +
<tr>
 +
  <td>Spec_rev</td>
 +
 
 +
  <td>GGAGCTAAGGAAGCTAAAATGAGCAATTTGATTAACGGA</td>
 +
</tr>
 +
<tr>
 +
  <td>Backbone_Spec_fw</td> 
 +
  <td>TATAGAAACTTCTCTCAATTAGTTTTTTTAAGGCAGTTATTGGT</td>
 +
</tr>
 +
<tr>
 +
  <td>Backbone_Spec_rev</td>
 +
  <td>TCCGTTAATCAAATTGCTCATTTTAGCTTCCTTAGCTCC</td>
 +
</tr>
 +
<tr>
 +
  <td>Pol_Backb_Fw</td>
 +
 
 +
  <td>CCGTTAATCAAATTGCTCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGAT</td>
 +
</tr>
 +
<tr>
 +
  <td>Pol_Backb_Rev</td>
 +
  <td>TAGAAACTTCTCTCAATTAGTTTTTTTAAGGCAGTTATTGGTGCCCTTAAACG</td>
 +
</tr>
 +
<tr>
 +
  <td>SpecR_Fw</td>
 +
  <td>CAGGAGCTAAGGAAGCTAAAATGAGCAATTTGATTAACGGAAAAATACCAAATCAAG</td>
 +
</tr>
 +
<tr>
 +
    <td>SpecR_Rev</td>
 +
  <td>CAATAACTGCCTTAAAAAAACTAATTGAGAGAAGTTTCTATAGAATTTTTCATATACTTA</td>
 +
</tr>
 +
<tr>
 +
  <td>EP_Cont_Fw</td>
 +
  <td>CGACTACTCGCAGAATGAACTGCG</td>
 +
</tr>
 +
<tr>
 +
  <td>WT_Cont_Fw</td>
 +
 +
  <td>GGACTACTCGCAGATTGAACTGCG</td>
 +
</tr>
 +
<tr>
 +
  <td>eStop_fw</td>
 +
  <td>ACACCAGCGTTTCTGGGTGAGCAAAAAC</td>
 +
</tr>
 +
<tr>
 +
  <td>eStop_rev</td>
 +
  <td>TCACCCAGAAACGCTGGTGTAAGTAAAAGATG</td>
 +
</tr>
 +
<tr>
 +
    <td>lStop_fw</td>
 +
  <td>TATACCGCGCCACATAGCAGAACTTTAAAAGT</td>
 +
</tr>
 +
<tr>
 +
  <td>lStop_rev</td>
 +
  <td>TCTGCTATGTGGCGCGGTATAATCCC</td>
 +
</tr>
 +
<tr>
 +
  <td>Gibs_PolI_fw</td>
 +
  <td>GATTTCTGGAATTCGCGGCCGCTTCTAGAGCAATACGCAAACCGCCTCTCCCC</td>
 +
</tr>
 +
<tr>
 +
  <td>Gibs_PolI_rev</td>
 +
  <td>TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAGTGCGCCTGATCCCAGTTTTCGC</td>
 +
</tr>
 +
<tr>
 +
  <td>Gibs_Pol_BB_fw</td>
 +
  <td>AGTGGCGAAAACTGGGATCAGGCGCACTAATACTAGTAGCGGCCGCTGCAGTC</td>
 +
</tr>
 +
<tr>
 +
  <td>Gibs_Pol_BB_rev</td>
 +
  <td>AACGCGCGGGGAGAGGCGGTTTGCGTATTGCTCTAGAAGCGGCCGCGAATTCCA</td>
 +
</tr>
 +
    </tbody>
 +
  </table>
 +
</div>
 +
<br><br>
 +
<button data-toggle="collapse" class="btn-block" data-target="#selection">Selection Primers</button>
 +
<div id="selection" class="collapse">
 +
<table class="table">
 +
    <thead>
 +
      <tr>
 +
        <th>Name</th>
 +
        <th>Sequence (5' &rarr; 3')</th>
 +
      </tr>
 +
    </thead>
 +
    <tbody>
 +
    <tr>
 +
 
 +
<td>pSB1C3_BB_fw</td>
 +
 
 +
<td>TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>pSB1C3_BB_fw_2</td>
 +
 
 +
<td>TAATACTAGTAGCGGCCGCTGCAGTCCG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>pSB1C3_BB_fw_Gal11P</td>
 +
 
 +
<td>GCTGCGCAAAATTCAGGCGCTGTAATACTAGTAGCGGCCGCTGCAGTCCG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>pSB1C3_BB_rv</td>
 +
 
 +
<td>CTCTAGAAGCGGCCGCGAATTCCAGAAATC</td>
 +
 
 +
</tr>
 +
<tr>
 +
 
 +
<td>pSB1C3_BB_rv_Gal11P</td>
 +
 
 +
<td>TTTGCTTTTTACCCTGGAAGAAATACTCATCTCTAGAAGCGGCCGCGAATTCCAGAAATC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Gal4_BB_fw</td>
 +
 
 +
<td>ATTAAAGCGCTGCTGACCGGCCTGTTTTAATACTAGAGAAAAAAAAACCCCGCC</td>
 +
 
 +
</tr>
 +
<tr>
 +
 
 +
<td>HA4_BB_fw</td>
 +
 
 +
<td>CCGATTAGCATTAACTATCGCACCTGCTAATACTAGAGAAAAAAAAACCCCGCC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Gal4_BB_HA4_rv</td>
 +
 
 +
<td>TACAGCGTCCTGAACAGTTACGCGTGCCATCTAGTATTTCTCCTCTTTCTCTAGTAGC</td>
 +
 
 +
</tr>
 +
<tr>
 +
 
 +
<td>Gal4_HA4_fw</td>
 +
 
 +
<td>TAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCACGCGTAACTGTTCA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Gal4_rv</td>
 +
 
 +
<td>GTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTAAAACAGGCCGGTCAGCA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>HA4_rv</td>
 +
 
 +
<td>GTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTAGCAGGTGCGATAGTTAATGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Gal11P_BB_fw</td>
 +
 
 +
<td>AAAGCGCTGCGCAAAATTCAGGCGCTGTAATACTAGAGAAAAAAAAACCCCGCC</td>
 +
 
 +
</tr>
 +
<tr>
 +
 
 +
<td>SH2_BB_fw</td>
 +
 
 +
<td>ACCGTGTATGGCGTGAGCCCGAACTATTAATACTAGAGAAAAAAAAACCCCGCC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Gal11P_SH2_BB_rv</td>
 +
 
 +
<td>TTTGCTTTTTACCCTGGAAGAAATACTCATCTAGTATTTCTCCTCTTTCTCTAGTAGCT</td>
 +
 
 +
</tr>
 +
<tr>
 +
 
 +
<td>Gal11P_SH2_fw</td>
 +
 
 +
<td>TAGCTACTAGAGAAAGAGGAGAAATACTAGATGAGTATTTCTTCCAGGGTAAAAAG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Gal11P_rv</td>
 +
 
 +
<td>GTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTACAGCGCCTGAATTTTGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>SH2_rv</td>
 +
 
 +
<td>GTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTAATAGTTCGGGCTCACGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Reporter_fw</td>
 +
 
 +
<td>ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATACAAGAAAGTTTGTTCATTAGGCAC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Reporter_rv</td>
 +
 
 +
<td>GGCGGGGTTTTTTTTTCTCTAGTATTAAGCACCGGTGGAGTGAC</td>
 +
 
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>B1006_BB_fw</td>
 +
 
 +
<td>GCTGAAGGTCGTCACTCCACCGGTGCTTAATACTAGAGAAAAAAAAACCCCGCCCCTGA</td>
 +
 
 +
</tr>
 +
<tr>
 +
 
 +
<td>B1006_BB_rv</td>
 +
 
 +
<td>TGAACAAACTTTCTTGTATTCTAGAAGCGGCCGCGAATTC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>B1006_BB_rv2</td>
 +
 
 +
<td>CTAGAAGCGGCCGCGAATTC</td>
 +
 
 +
</tr>
 +
<tr>
 +
 
 +
<td>Reporter_SH2_Gal11P_BB_fw</td>
 +
 
 +
<td>CTGACAGGGCGGGGTTTTTTTTTACTAGAGTTTACGGCTAGCTCAGTCCTAGGT</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Reporter_SH2_Gal11P_BB_rv</td>
 +
 
 +
<td>GGGTGCCTAATGAACAAACTTTCTTGTATTCTCTAGAAGCGGCCGCGAAT</td>
 +
 
 +
</tr>
 +
<tr>
 +
 
 +
<td>Reporter_SH2_Gal11P_fw</td>
 +
 
 +
<td>AATACAAGAAAGTTTGTTCATTAGGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Reporter_SH2_Gal11P_rv</td>
 +
 
 +
<td>CTAGGACTGAGCTAGCCGTAAACTCTAGTAAAAAAAAACCCCGCCCTG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>IMP_BB_fw</td>
 +
 
 +
<td>TGCATCACGGGAGATGC</td>
 +
 
 +
</tr>
 +
<tr>
 +
 
 +
<td>IMP_BB_GCG_fw</td>
 +
 
 +
<td>GCGTGCATCACGGGAGATGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>IMP_BB_rv</td>
 +
 
 +
<td>CATATGTATATCTCCTTCTTAAAGTTAAACAA</td>
 +
 
 +
</tr>
 +
<tr>
 +
 
 +
<td>IMP_HA4FP_fw</td>
 +
 
 +
<td>TTGTTTAACTTTAAGAAGGAGATATACATATGGCACGCGTAACTGTTCAGG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>IMP_HA4FP_rv</td>
 +
 
 +
<td>TAGGGCAACTAGTGCATCTCCCGTGATGCACGCGCAGGTGCGATAGTTAATGCTAAT</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>IMP_SH2FP_fw</td>
 +
 
 +
<td>TTGTTTAACTTTAAGAAGGAGATATACATATGAGTATTTCTTCCAGGGTAAAAAGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>IMP_SH2FP_rv</td>
 +
 
 +
<td>TAGGGCAACTAGTGCATCTCCCGTGATGCAATAGTTCGGGCTCACGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>IMP_cI_lambda_fw</td>
 +
 
 +
<td>TTGTTTAACTTTAAGAAGGAGATATACATATGAGCACAAAAAAGAAACCATTAACACAAG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>IMP_cI_lambda_rv</td>
 +
 
 +
<td>TAGGGCAACTAGTGCATCTCCCGTGATGCAAAACGTCTCTTCAGGCCACTG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_BB_FragA_fw</td>
 +
 
 +
<td>TTCACCTGTGGAGCTTTTTAAGTTACTAGTAGCGGCCGCTGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_BB_FragA_rv</td>
 +
 
 +
<td>TGCTGGCAATCATCTGTGTGCTCTAGAAGCGGCCGCGA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_BB_FragAB_fw</td>
 +
 
 +
<td>CGAAGATATGGACCAGATGGCGGTACTAGTAGCGGCCGCTGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_BB_FragAB_rv</td>
 +
 
 +
<td>GGGCGACCCGCTTTGTGAACTTAAAAAGCTCCACAGGTGAAGAAAT</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_BB_FragAB_Reporter_fw</td>
 +
 
 +
<td>ACAGGGCGGGGTTTTTTTTTCACAAAGCGGGTCGCCC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_BB_FragAB_Reporter_rv</td>
 +
 
 +
<td>TGCCTAATGAACAAACTTTCTTGTATTACTTAAAAAGCTCCACAGGTGAAGAAATG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_FragA_fw</td>
 +
 
 +
<td>TCGCGGCCGCTTCTAGAGCACACAGATGATTGCCAGCA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_FragA_rv</td>
 +
 
 +
<td>GCAGCGGCCGCTACTAGTAACTTAAAAAGCTCCACAGGTGAA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_BB_FragA_rv</td>
 +
 
 +
<td>TGCTGGCAATCATCTGTGTGCTCTAGAAGCGGCCGCGA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_BB_FragAB_fw</td>
 +
 
 +
<td>CGAAGATATGGACCAGATGGCGGTACTAGTAGCGGCCGCTGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_BB_FragAB_rv</td>
 +
 
 +
<td>GGGCGACCCGCTTTGTGAACTTAAAAAGCTCCACAGGTGAAGAAAT</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_BB_FragAB_Reporter_fw</td>
 +
 
 +
<td>ACAGGGCGGGGTTTTTTTTTCACAAAGCGGGTCGCCC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_BB_FragAB_Reporter_rv</td>
 +
 
 +
<td>TGCCTAATGAACAAACTTTCTTGTATTACTTAAAAAGCTCCACAGGTGAAGAAATG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_FragA_fw</td>
 +
 
 +
<td>TCGCGGCCGCTTCTAGAGCACACAGATGATTGCCAGCA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_FragA_rv</td>
 +
 
 +
<td>GCAGCGGCCGCTACTAGTAACTTAAAAAGCTCCACAGGTGAA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_FragB_fw</td>
 +
 
 +
<td>ATTTCTTCACCTGTGGAGCTTTTTAAGTTCACAAAGCGGGTCGCCC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_FragB_rv</td>
 +
 
 +
<td>GCAGCGGCCGCTACTAGTACCGCCATCTGGTCCATATCTTCG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_Reporter_fw</td>
 +
 
 +
<td>CATTTCTTCACCTGTGGAGCTTTTTAAGTAATACAAGAAAGTTTGTTCATTAGGCA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_Reporter_rv</td>
 +
 
 +
<td>GGGCGACCCGCTTTGTGAAAAAAAAACCCCGCCCTGT</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_FragAB_Reporter_fw</td>
 +
 
 +
<td>CACACAGATGATTGCCAGCA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CC_FragAB_Reporter_rv</td>
 +
 
 +
<td>CCGCCATCTGGTCCATATCTTCG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CI_lambda_part1_fw</td>
 +
 
 +
<td>TTTACTATCACCGCCAGAGGTAACGTACATTAGGCACCCCGGGCTT</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CI_lambda_part2_fw</td>
 +
 
 +
<td>AGTGGCCTGAAGAGACGTTTGAACAAAAGCTTATTTCTGAAGAGGA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CI_lambda_part1_rv</td>
 +
 
 +
<td>TGTGTTAATGGTTTCTTTTTTGTGCTCTAGTATTTCTCCTCTTTCTCTAGTAGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CI_lambda_part2_rv</td>
 +
 
 +
<td>TACGTTACCTCTGGCGGTGATAGTAAACTCTAGAAGCGGCCGCGAA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CI_lambda_Insert_fw</td>
 +
 
 +
<td>GCTACTAGAGAAAGAGGAGAAATACTAGAGCACAAAAAAGAAACCATTAACACA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>CI_lambda_Insert_rv</td>
 +
 
 +
<td>TCCTCTTCAGAAATAAGCTTTTGTTCAAACGTCTCTTCAGGCCACT</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>RpoA_BB_fw</td>
 +
 
 +
<td>GCAAGCATCGCTGACGAGGAACAAAAGCTTATTTCTGAAGAGGA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>RpoA_BB_rv</td>
 +
 
 +
<td>TCTGTCACAGAACCCTGCATCTAGTATTTCTCCTCTTTCTCTAGTAGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>RpoA_fw</td>
 +
 
 +
<td>GCTACTAGAGAAAGAGGAGAAATACTAGATGCAGGGTTCTGTGACAGA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>RpoA_rv</td>
 +
 
 +
<td>CAAGTCCTCTTCAGAAATAAGCTTTTGTTCCTCGTCAGCGATGCTTGC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Tet_BB_fw</td>
 +
 
 +
<td>TCGACCTAATACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTAT</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Tet_BB_rv</td>
 +
 
 +
<td>CGATGAGCGCATTGTTAGATTTCATCTAGTATTTCTCCTCTTTCTCTAGTACGGT</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Tet_fw</td>
 +
 
 +
<td>ACCGTACTAGAGAAAGAGGAGAAATACTAGATGAAATCTAACAATGCGCTCATCG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Tet_rv</td>
 +
 
 +
<td>GGAAGCCATCTAGTATTTCTCCTCTTTCTCTAGTATTAGGTCGAGGTGGCCCG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>OR2_cI_434_fw</td>
 +
 
 +
<td>CGCTTCTAGAGACAAGATACATTGTATGAAAATACAAGAAAGTTTGTTCATTAGGCACC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>OR2_cI_434_rv</td>
 +
 
 +
<td>TTTCTTGTATTTTCATACAATGTATCTTGTCTCTAGAAGCGGCCGCGA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Seq_Reporter_fw</td>
 +
 
 +
<td>AAGGTTTCAAATGGGAACGTG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Seq_cI_434_rv</td>
 +
 
 +
<td>CACCACGAACCAGCTCTAA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Seq_cI_lambda_rv</td>
 +
 
 +
<td>CGGAACAACTCTCATTGCATG</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Seq_FragAB_Reporter_fw1</td>
 +
 
 +
<td>CAGGTTTCTGTTTCACACACC</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Seq_FragAB_Reporter_fw2</td>
 +
 
 +
<td>TCTTCACCTGTGGAGCTTTTT</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Seq_FragAB_Reporter_rv1</td>
 +
 
 +
<td>TTACGCGATAACGGTTGGGA</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
 
 +
<td>Seq_FragAB_Reporter_rv2</td>
 +
 
 +
<td>GCAGGTAGGTTTGAATCAGTTG</td>
 +
 
 +
</tr>
 +
 
 +
</tbody>
 +
 
 +
</table>
 +
 
 +
</div>
 +
 
 +
<br><br><br>
 +
 
 +
 
 +
 
 +
</div>
 +
 
 +
</div>
 +
 
 +
<script type="text/javascript" src="https://2016.igem.org/wiki/index.php?title=Template:Team:Bielefeld-CeBiTec/styles/js&amp;action=raw&amp;ctype=text/javascript"></script>
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Latest revision as of 22:30, 19 October 2016



Primers

Primed for success

Primers


Name Sequence (5' → 3')
VF CCACCTGACGTCTAAGAAAC
VR GTATTACCGCCTTTGAGTGAGC


Name Sequence (5' → 3')
Target_bb_fw TACTAGAGAAAAAAAAACCCCGCCCC
Target_bb_rev CAAGTCCTCTTCAGAAATAAGCTTTTGTTC
MB-Insert-fw GAACAAAAGCTTATTTCTGAAGAGGACTTGGTTTCTTCTGTTCCGACCAAACTGG
MB-Insert-rev GTCAGGGGCGGGGTTTTTTTTTCTCTAGTAGGTACGGTAGTTGATAGAGATCGGA
NB-Insert-fw GAACAAAAGCTTATTTCTGAAGAGGACTTGCAGGTTCAGCTGGTTGAATCTGGT
NB-Insert-rev GGGCGGGGTTTTTTTTTCTCTAGTAAGCAGCGCAGTAGTAGATAGCGG
NB-bb-fw GGGTACGCAGGTTACCGTTTCTTCTTACTAGAGAAAAAAAAACCCCGCCCC
NB-bb-rev AGCAGCGCAGTAGTAGATAGCGG
MB-bb-fw TCTCCGATCTCTATCAACTACCGTAC
MB-bb-rev AACGGTAACAGCCGGAGCGTC
NB-Control-fw ACACCGTTACCCTGCAAATGAACAAC
NB-control-rev ACTGCAGCGGCCGCTACTAGTA
NB-psb-I1-fw GGAATTCGCGGCCGCTTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACT
NB-psb-I1-rev GTACCTAGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCAT
NB-psb-I2-fw CCCTGACAGGGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAGTCCGGCAAA
NB-psb-I2-rev GACTGCAGCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCG
MB-psb-1-fw GGAATTCGCGGCCGCTTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTAC
MB-psb-1-rev GTACCTAGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCT
MB-psb-3-fw CTACCCCGACCTCTCTGCTGATCTCTTGGGACGCTCCGGCTGTTACCGTT
MB-psb-3-rev GTAACAGCCGGAGCGTCCCAAGAGATCAGCAGAGAGGTCGGGGTAGCAGCA
MB-psb-4-fw CTCCGATCTCTATCAACTACCGTACCAAAAAAAAACCCCGCCCCTGACAGGGCGGG
MB-psb-4-rev CCTGTCAGGGGCGGGGTTTTTTTTTGGTACGGTAGTTGATAGAGATCGGAGA
BIFC_GFP_contr_fw ATGTCTAAAGGTGAAGAACTGTTCACCG
BIFC_GFP_contr_rev TCCGGAACGTCGTACGGGTACAT
BIFC_BINDER_contr_fw GTTCAGCTGGTTGAATCTGGTGGT
BIFC_BINDER_contr_rev TTCGATGTTGTGACGGATTTTGAAGTTAGC
LIB-bb-fw CTGGAATTCGCGGCCGCTTCTAGAG
LIB-bb-rev CTCTAGAAGCGGCCGCGAATTCCAG
Phag_MB_pAK100blaKDIR_fw CGCTGGATTGTTATTACTCGCGGCCCAGCCGGCCTTTGCTGTTTCTTCTGTTCCGACCAAACTGGAA
Phag_MB_pAK100_rev CTTCTGCTCGAATTCGGCCCCCGAGGCCGAGTAGGTACGGTAGTTGATAGAGATCG
Phag_NB_pAK100blaKDIR _fw CGCTGGATTGTTATTACTCGCGGCCCAGCCGGCCTTTGCTCAGGTTCAGCTGGTTGAATCTGGT
Phag_MB_pAK100_rev CTTCTGCTCGAATTCGGCCCCCGAGGCCGAGTAAGAAGAAACGGTAACCTGCGTAC
Zk_Seq_fw1 CGTGGTGTGAAGCTGAACAAAAGCTT
Zk_Seq_rev1 AATCCCACGCGGTATCGCCCA
Zk_Seq_fw2 TGCATGGCAGCCAGCATAGCG
Zk_Seq_rev2 CATTTCCGCTTCCAGCGCGC
Zk_Insert_pSB1C3_fw GATTTCTGGAATTCGCGGCCGCTTCTAGAGATTAGATGTATCGGCGTAAGTAATCGCG
Zk_Insert_pSB1C3_rev TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAGGCAGAGACCGCAGTTGACAAAAA
Zk_cMyc_BB_fw ATCTTTTTGTCAACTGCGGTCTCTGCCTAATACTAGAGAAAAAAAAACCCCGCCCCT
Zk_cMyc_BB_rev GTCGCGATTACTTACGCCGATACATCTAATCAAGTCCTCTTCAGAAATAAGCTTTTGTTCA


Name Sequence (5' → 3')
emrR_Gib_fw GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCACACAGGAAACCTACTAGATGGATAGTTCGTTTACGCCC
emrR_Gib_rev TAAATTTGTCATCTAGTAGGTTTCCTGTGTGATTATTAGCTCATCGCTTCGAGAAC
cPCR_ugi_cda1 CTGGGGTTACGCGGTTAAC
ugi_Gib_fw GCGATGAGCTAATAATCACACAGGAAACCTACTAGATGACAAATTTATCTGACATCATTGA
ugi_Gib_rev CGCGTCGGTCATCTAGTAGGTTTCCTGTGTGATTATTATAACATTTTAATTTTATTTTCTCCATTACT
ugi-cda1_Gib_fw AAAATGTTATAATAATCACACAGGAAACCTACTAGATGACCGACGCGGAATAC
ugi-cda1_Gib_rev GACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAAACCGCCGGAGATTTGG
WT_PolI_Gib_for ACCATGATTACGCCAAGCTTATGGTTCAGATCCCCCAAAA
WT_PolI_Gib_rev AACGACGGCCAGTGGAATTCTTAGTGCGCCTGATCCCAGT
pEPPolI_Gib_for GAATTCCACTGGCCGTCGTT
pEPPolI_Gib_rev TTTTGGGGGATCTGAACCATAAGC
pEPPolI_Seq_for GCACCCCAGGCTTTACAC
pEPPolI_Seq_rev GGGTAACGCCAGGGTTTT
pEPPolI_Col_rev GCGGGCGATGTGATTTGT
pSB1K3_Del_for GCTGAGTTGAAGGATCAGCTCAGGAAAGAACATGTGAGCA
pSB1K3_Del_rev TGCTCACATGTTCTTTCCTGAGCTGATCCTTCAACTCAGC
Sbla_T1_Gib_for GGTTCCTGGCCTTTTGCTGGCCTATTTGTTTATTTTTCTA
Sbla_T1_Gib_rev ATCTTCAGCATCTTTTACTTACACCAGCGTTTCTGGGTGA
Sbla_T2_Gib_for TCACCCAGAAACGCTGGTGTAAGTAAAAGATGCTGAAGAT
Sbla_T2_Gib_rev AAGAACATGTGAGCAAAAGGATATGAGTAAACTTGGTCTG
pSB1K3D_Gib_for CAGACCAAGTTTACTCATATCCTTTTGCTCACATGTTCTT
pSB1K3D_Gib_rev TAGAAAAATAAACAAATAGGCCAGCAAAAGGCCAGGAACC
Sbla_EndSeq_rev GCCTGGAGCAAGACGTTT
pSB1K3D_Col_for ACACAACGTGGCTTTGTTG
pSB1K3D_Col_rev GGTTCCTGGCCTTTTGCT
Sbla_Col_for GGTTTCGCCACCTCTGAC
pSB1K3_D_for_P2 TCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTC
pSB1K3_D_rev_P1 GGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGA
RBS_RFP_Ter_for TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG
RBS_RFP_Ter_rev CTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTATTAAGCACCGGTGGAGTGA
C3BB_Ter_for TAGAGAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAGTCCG
C3BB_And_01_rev CTCTTTCTCTAGTAGCTAGCATTGTACCTAGGACTGAGCTAGCCATAAACTCTAGAAGCGGCCGCGAATTCCAGAAATCA
C3BB_And_047_rev CTCTTTCTCTAGTAGCTAGCACTATACCTAGGACTGAGCTAGCCGTAAACTCTAGAAGCGGCCGCGAATTCCAGAAATCA
C3BB_And_086_rev CTCTTTCTCTAGTAGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCA
VF2 TGCCACCTGACGTCTAAGAA
VR ATTACCGCCTTTGAGTGAGC
BFP_Gib_for ACATACTAGAGAAAGAGGAGAAATACTAGATGAGCGAACTGATCAAAGAGAA
BFP_Gib_rev GATCTACACTAGCACTATCAGCGTTATTAATTCAGTTTATGACCCAGCTTG
K3_BFP_Gib_for CAAGCTGGGTCATAAACTGAATTAATAACGCTGATAGTGCTAGTGTAGATC
K3_BFP_Gib_rev TTCTCTTTGATCAGTTCGCTCATCTAGTATTTCTCCTCTTTCTCTAGTATGT
Web_C3BB_for GCCGCTGCAGTCCGGCAAAAAAG
Web_C3BB_rev GCCGCGAATTCCAGAAATCATCCTTAGCG
pSB1K3_D_rev_P2 TGCTCACATGTTCTTTCCTGCTCTGATGTTACATTGCACAAGATA
cPCR_dnaQ_for GGTCACCGATAGCCTTGC
cPCR_ugi-cda1_for CTGGGGTTACGCGGTTAAC
dnaQ_Gib_for GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCACACAGGAAACCTACTAGATGAGCACTGCAATTACACGC
dnaQ_Gib_rev ATTTTTCTTCATCTAGTAGGTTTCCTGTGTGATTATTATGCTCGCCAGAGGCAAC
dam_Gib_for TGGCGAGCATAATAATCACACAGGAAACCTACTAGATGAAGAAAAATCGCGCTTTTTTG
dam_Gib_rev TGGCGAGCATAATAATCACACAGGAAACCTACTAGATGAAGAAAAATCGCGCTTTTTTG
seqA_Gib_for GCGAAAAAATAATAATCACACAGGAAACCTACTAGATGAAAACGATTGAAGTTGATGATG
seqA_Gib_rev TGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAGATAGTTCCGCAAACCTTCTC
cPCR_dam_for GGAAGCAGAGTTGTATCACTTCG
PolI_Gib_for GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGTTCAGATCCCCCAAAATC
PolI_Gib_rev TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAGTGCGCCTGATCCCAG
E0040_A68T_Gib_for GGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAG
E0040_A68T_Gib_rev GTGAAAAGTTCTTCTCCTTTACGCATCTAGTATTTCTCCTCTTTC
E0040_C379A_Gib_for TCAAAGATGACGGGAACTAAAAGACACGTGCTGAAGTCAA
E0040_C379A_Gib_rev TGACTTCAGCACGTGTCTTTTAGTTCCCGTCATCTTTGAAAAATATAG
E0040_A380T_Gib_for CAAAGATGACGGGAACTACTAGACACGTGCTGAAGTCAAG
E0040_A380T_Gib_rev TTGACTTCAGCACGTGTCTAGTAGTTCCCGTCATCTTTGAAAAAT
GFP_MutSeq_for GATGCAACATACGGAAAACTTACC
Pbad_Change_for CCGTCCACCCCCTAATATCCCGACATACCCGTTTTTTGGGCTAACT
Pbad_Change_rev GATATTAGGGGGTGGACGGGGGGGGCGTCAGGTAGGATCCGC
Pbad_Conf_rev GGATATTAGGGGGTGGACGG
dnaQ_Exp_for TCACACAGGAAACCTACTAGATGAGCACTGCAATTACACGC
dnaQ_Exp_rev CTACACTAGCACTATCAGCGTTATGCTCGCCAGAGGCAAC
dnaQ_ExpBB_for GTTGCCTCTGGCGAGCATAACGCTGATAGTGCTAGTGTAGATC
dnaQ_ExpBB_rev CGTGTAATTGCAGTGCTCATCTAGTAGGTTTCCTGTGTGACTC
PolI_Exp_for TCACACAGGAAACCTACTAGATGGTTCAGATCCCCCAAAATC
PolI_Exp_rev CTACACTAGCACTATCAGCGTTAGTGCGCCTGATCCCA
PolI_Exp_rev ACTGGGATCAGGCGCACTAACGCTGATAGTGCTAGTGTAGATC
PolI_ExpBB_rev TTTTGGGGGATCTGAACCATCTAGTAGGTTTCCTGTGTGACTC
PolI_Seq_A ACGCTTATCAATACCATGACG
PolI_Seq_B TGACAACTACGTCACCATCC
PolI_Seq_C CGTTCGAAGTAAAGGTCCATG
SGFP_A_for GGAGAAATACTAGATGCGTTAAGGAGAAGAACTTTTCACTGGAG
SGFP_A_rev GTGAAAAGTTCTTCTCCTTAACGCATCTAGTATTTCTCCTCTTTC
ugi_Single_for TAAAATTAAAATGTTATAATACTAGTAGCGGCCGCT
ugi_Single_rev CGCTACTAGTATTATAACATTTTAATTTTATTTTCTCCATTACTGTC
cda1_Single_for CTTCTAGAGATGACCGACGCGGAATACG
cda1_Single_rev GCGTCGGTCATCTCTAGAAGCGGCCGC
dam_Single_for CCGCGAAAAAATAATACTAGTAGCGGCCGCT
dam_Single_rev CTACTAGTATTATTTTTTCGCGGGTGAAACG
seqA_Single_for GCTTCTAGAGATGAAAACGATTGAAGTTGATGATGAACT
SeqA_Single_for CAATCGTTTTCATCTCTAGAAGCGGCCGC
MP6_ExpBB_for CCAAATCTCCGGCGGTTTAACGCTGATAGTGCTAGTGTAGATC
MP6_Exp_rev CTACACTAGCACTATCAGCGTTAAACCGCCGGAGATTTGGT
B0031_Conf_for TCACACAGGAAACCTACTAGATG
AraProm_VF CGGGACCAAAGCCATGAC
VF2 TGCCACCTGACGTCTAAGAA
VR ATTACCGCCTTTGAGTGAGC
araE_Ins_for AGAAAGAGGAGAAATACTAGATGGTTACTATCAATACGGAATCTGC
araE_Ins_rev GGGTTTTTTTTTCTCTAGTATCAGACGCCGATATTTCTCAAC
araE_BB_for TGAGAAATATCGGCGTCTGATACTAGAGAAAAAAAAACCCCGCC
araE_BB_rev TCCGTATTGATAGTAACCATCTAGTATTTCTCCTCTTTCTCTAGTAGC
Bb_site_fw CGCTTCTAGA GTACTAGTAGCGGCCGCTGCAGGGGTGCTACTTAAGCCTTTAGG
Bb_site_rev CTACTAGTACTCTAGAAGCGGCCGCGAATTCTCGCAAGCTCGGGCAAATCG
Spec_fw ACCAATAACTGCCTTAAAAAAACTAATTGAGAGAAGTTTCTATA
Spec_rev GGAGCTAAGGAAGCTAAAATGAGCAATTTGATTAACGGA
Backbone_Spec_fw TATAGAAACTTCTCTCAATTAGTTTTTTTAAGGCAGTTATTGGT
Backbone_Spec_rev TCCGTTAATCAAATTGCTCATTTTAGCTTCCTTAGCTCC
Pol_Backb_Fw CCGTTAATCAAATTGCTCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGAT
Pol_Backb_Rev TAGAAACTTCTCTCAATTAGTTTTTTTAAGGCAGTTATTGGTGCCCTTAAACG
SpecR_Fw CAGGAGCTAAGGAAGCTAAAATGAGCAATTTGATTAACGGAAAAATACCAAATCAAG
SpecR_Rev CAATAACTGCCTTAAAAAAACTAATTGAGAGAAGTTTCTATAGAATTTTTCATATACTTA
EP_Cont_Fw CGACTACTCGCAGAATGAACTGCG
WT_Cont_Fw GGACTACTCGCAGATTGAACTGCG
eStop_fw ACACCAGCGTTTCTGGGTGAGCAAAAAC
eStop_rev TCACCCAGAAACGCTGGTGTAAGTAAAAGATG
lStop_fw TATACCGCGCCACATAGCAGAACTTTAAAAGT
lStop_rev TCTGCTATGTGGCGCGGTATAATCCC
Gibs_PolI_fw GATTTCTGGAATTCGCGGCCGCTTCTAGAGCAATACGCAAACCGCCTCTCCCC
Gibs_PolI_rev TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAGTGCGCCTGATCCCAGTTTTCGC
Gibs_Pol_BB_fw AGTGGCGAAAACTGGGATCAGGCGCACTAATACTAGTAGCGGCCGCTGCAGTC
Gibs_Pol_BB_rev AACGCGCGGGGAGAGGCGGTTTGCGTATTGCTCTAGAAGCGGCCGCGAATTCCA


Name Sequence (5' → 3')
pSB1C3_BB_fw TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG
pSB1C3_BB_fw_2 TAATACTAGTAGCGGCCGCTGCAGTCCG
pSB1C3_BB_fw_Gal11P GCTGCGCAAAATTCAGGCGCTGTAATACTAGTAGCGGCCGCTGCAGTCCG
pSB1C3_BB_rv CTCTAGAAGCGGCCGCGAATTCCAGAAATC
pSB1C3_BB_rv_Gal11P TTTGCTTTTTACCCTGGAAGAAATACTCATCTCTAGAAGCGGCCGCGAATTCCAGAAATC
Gal4_BB_fw ATTAAAGCGCTGCTGACCGGCCTGTTTTAATACTAGAGAAAAAAAAACCCCGCC
HA4_BB_fw CCGATTAGCATTAACTATCGCACCTGCTAATACTAGAGAAAAAAAAACCCCGCC
Gal4_BB_HA4_rv TACAGCGTCCTGAACAGTTACGCGTGCCATCTAGTATTTCTCCTCTTTCTCTAGTAGC
Gal4_HA4_fw TAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCACGCGTAACTGTTCA
Gal4_rv GTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTAAAACAGGCCGGTCAGCA
HA4_rv GTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTAGCAGGTGCGATAGTTAATGC
Gal11P_BB_fw AAAGCGCTGCGCAAAATTCAGGCGCTGTAATACTAGAGAAAAAAAAACCCCGCC
SH2_BB_fw ACCGTGTATGGCGTGAGCCCGAACTATTAATACTAGAGAAAAAAAAACCCCGCC
Gal11P_SH2_BB_rv TTTGCTTTTTACCCTGGAAGAAATACTCATCTAGTATTTCTCCTCTTTCTCTAGTAGCT
Gal11P_SH2_fw TAGCTACTAGAGAAAGAGGAGAAATACTAGATGAGTATTTCTTCCAGGGTAAAAAG
Gal11P_rv GTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTACAGCGCCTGAATTTTGC
SH2_rv GTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTAATAGTTCGGGCTCACGC
Reporter_fw ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATACAAGAAAGTTTGTTCATTAGGCAC
Reporter_rv GGCGGGGTTTTTTTTTCTCTAGTATTAAGCACCGGTGGAGTGAC
B1006_BB_fw GCTGAAGGTCGTCACTCCACCGGTGCTTAATACTAGAGAAAAAAAAACCCCGCCCCTGA
B1006_BB_rv TGAACAAACTTTCTTGTATTCTAGAAGCGGCCGCGAATTC
B1006_BB_rv2 CTAGAAGCGGCCGCGAATTC
Reporter_SH2_Gal11P_BB_fw CTGACAGGGCGGGGTTTTTTTTTACTAGAGTTTACGGCTAGCTCAGTCCTAGGT
Reporter_SH2_Gal11P_BB_rv GGGTGCCTAATGAACAAACTTTCTTGTATTCTCTAGAAGCGGCCGCGAAT
Reporter_SH2_Gal11P_fw AATACAAGAAAGTTTGTTCATTAGGC
Reporter_SH2_Gal11P_rv CTAGGACTGAGCTAGCCGTAAACTCTAGTAAAAAAAAACCCCGCCCTG
IMP_BB_fw TGCATCACGGGAGATGC
IMP_BB_GCG_fw GCGTGCATCACGGGAGATGC
IMP_BB_rv CATATGTATATCTCCTTCTTAAAGTTAAACAA
IMP_HA4FP_fw TTGTTTAACTTTAAGAAGGAGATATACATATGGCACGCGTAACTGTTCAGG
IMP_HA4FP_rv TAGGGCAACTAGTGCATCTCCCGTGATGCACGCGCAGGTGCGATAGTTAATGCTAAT
IMP_SH2FP_fw TTGTTTAACTTTAAGAAGGAGATATACATATGAGTATTTCTTCCAGGGTAAAAAGC
IMP_SH2FP_rv TAGGGCAACTAGTGCATCTCCCGTGATGCAATAGTTCGGGCTCACGC
IMP_cI_lambda_fw TTGTTTAACTTTAAGAAGGAGATATACATATGAGCACAAAAAAGAAACCATTAACACAAG
IMP_cI_lambda_rv TAGGGCAACTAGTGCATCTCCCGTGATGCAAAACGTCTCTTCAGGCCACTG
CC_BB_FragA_fw TTCACCTGTGGAGCTTTTTAAGTTACTAGTAGCGGCCGCTGC
CC_BB_FragA_rv TGCTGGCAATCATCTGTGTGCTCTAGAAGCGGCCGCGA
CC_BB_FragAB_fw CGAAGATATGGACCAGATGGCGGTACTAGTAGCGGCCGCTGC
CC_BB_FragAB_rv GGGCGACCCGCTTTGTGAACTTAAAAAGCTCCACAGGTGAAGAAAT
CC_BB_FragAB_Reporter_fw ACAGGGCGGGGTTTTTTTTTCACAAAGCGGGTCGCCC
CC_BB_FragAB_Reporter_rv TGCCTAATGAACAAACTTTCTTGTATTACTTAAAAAGCTCCACAGGTGAAGAAATG
CC_FragA_fw TCGCGGCCGCTTCTAGAGCACACAGATGATTGCCAGCA
CC_FragA_rv GCAGCGGCCGCTACTAGTAACTTAAAAAGCTCCACAGGTGAA
CC_BB_FragA_rv TGCTGGCAATCATCTGTGTGCTCTAGAAGCGGCCGCGA
CC_BB_FragAB_fw CGAAGATATGGACCAGATGGCGGTACTAGTAGCGGCCGCTGC
CC_BB_FragAB_rv GGGCGACCCGCTTTGTGAACTTAAAAAGCTCCACAGGTGAAGAAAT
CC_BB_FragAB_Reporter_fw ACAGGGCGGGGTTTTTTTTTCACAAAGCGGGTCGCCC
CC_BB_FragAB_Reporter_rv TGCCTAATGAACAAACTTTCTTGTATTACTTAAAAAGCTCCACAGGTGAAGAAATG
CC_FragA_fw TCGCGGCCGCTTCTAGAGCACACAGATGATTGCCAGCA
CC_FragA_rv GCAGCGGCCGCTACTAGTAACTTAAAAAGCTCCACAGGTGAA
CC_FragB_fw ATTTCTTCACCTGTGGAGCTTTTTAAGTTCACAAAGCGGGTCGCCC
CC_FragB_rv GCAGCGGCCGCTACTAGTACCGCCATCTGGTCCATATCTTCG
CC_Reporter_fw CATTTCTTCACCTGTGGAGCTTTTTAAGTAATACAAGAAAGTTTGTTCATTAGGCA
CC_Reporter_rv GGGCGACCCGCTTTGTGAAAAAAAAACCCCGCCCTGT
CC_FragAB_Reporter_fw CACACAGATGATTGCCAGCA
CC_FragAB_Reporter_rv CCGCCATCTGGTCCATATCTTCG
CI_lambda_part1_fw TTTACTATCACCGCCAGAGGTAACGTACATTAGGCACCCCGGGCTT
CI_lambda_part2_fw AGTGGCCTGAAGAGACGTTTGAACAAAAGCTTATTTCTGAAGAGGA
CI_lambda_part1_rv TGTGTTAATGGTTTCTTTTTTGTGCTCTAGTATTTCTCCTCTTTCTCTAGTAGC
CI_lambda_part2_rv TACGTTACCTCTGGCGGTGATAGTAAACTCTAGAAGCGGCCGCGAA
CI_lambda_Insert_fw GCTACTAGAGAAAGAGGAGAAATACTAGAGCACAAAAAAGAAACCATTAACACA
CI_lambda_Insert_rv TCCTCTTCAGAAATAAGCTTTTGTTCAAACGTCTCTTCAGGCCACT
RpoA_BB_fw GCAAGCATCGCTGACGAGGAACAAAAGCTTATTTCTGAAGAGGA
RpoA_BB_rv TCTGTCACAGAACCCTGCATCTAGTATTTCTCCTCTTTCTCTAGTAGC
RpoA_fw GCTACTAGAGAAAGAGGAGAAATACTAGATGCAGGGTTCTGTGACAGA
RpoA_rv CAAGTCCTCTTCAGAAATAAGCTTTTGTTCCTCGTCAGCGATGCTTGC
Tet_BB_fw TCGACCTAATACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTAT
Tet_BB_rv CGATGAGCGCATTGTTAGATTTCATCTAGTATTTCTCCTCTTTCTCTAGTACGGT
Tet_fw ACCGTACTAGAGAAAGAGGAGAAATACTAGATGAAATCTAACAATGCGCTCATCG
Tet_rv GGAAGCCATCTAGTATTTCTCCTCTTTCTCTAGTATTAGGTCGAGGTGGCCCG
OR2_cI_434_fw CGCTTCTAGAGACAAGATACATTGTATGAAAATACAAGAAAGTTTGTTCATTAGGCACC
OR2_cI_434_rv TTTCTTGTATTTTCATACAATGTATCTTGTCTCTAGAAGCGGCCGCGA
Seq_Reporter_fw AAGGTTTCAAATGGGAACGTG
Seq_cI_434_rv CACCACGAACCAGCTCTAA
Seq_cI_lambda_rv CGGAACAACTCTCATTGCATG
Seq_FragAB_Reporter_fw1 CAGGTTTCTGTTTCACACACC
Seq_FragAB_Reporter_fw2 TCTTCACCTGTGGAGCTTTTT
Seq_FragAB_Reporter_rv1 TTACGCGATAACGGTTGGGA
Seq_FragAB_Reporter_rv2 GCAGGTAGGTTTGAATCAGTTG