Difference between revisions of "Team:Bielefeld-CeBiTec/Experiments/Primers"

 
(3 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<body>
 
<body>
 
<div class="container main">
 
<div class="container main">
 +
<div class="jumbotron" style="background-image: url(https://static.igem.org/mediawiki/2016/a/ab/Bielefeld_CeBiTec_2016_10_14_X_notebook.png)">
 +
<div class="jumbotron-text">
 +
<h1 style="margin-bottom: 0px; text-align:left">Primers</h1>
 +
<h2 style="color:#ffffff; text-align:left">Primed for success</h2>
 +
</div>
 +
</div>
 +
<p style="position: relative; font-size: 9px; top: -50px; color: white; float: right; right: 10px;"></p>
 
 
 
 
−
<div class="container text_header"><h1>primers.</h1></div>
+
<div class="container text_header"><h3>Primers</h3></div>
−
<br>
+
−
<div class="container text_header"><h3>Primed for success</h3></div>
+
 
<br>
 
<br>
−
<div class="container text"><button data-toggle="collapse" data-target="#standard">Standard Primers</button >
+
<div class="container text"><button data-toggle="collapse" class="btn-block" data-target="#standard">Standard Primers</button >
 
<div id="standard" class="collapse">
 
<div id="standard" class="collapse">
 
  <table class="table">
 
  <table class="table">
Line 31: Line 36:
 
</div>
 
</div>
 
<br><br>
 
<br><br>
−
<button data-toggle="collapse" data-target="#library">Library Primers</button>
+
<button data-toggle="collapse" class="btn-block" data-target="#library">Library Primers</button>
 
<div id="library" class="collapse">
 
<div id="library" class="collapse">
 
  <table class="table">
 
  <table class="table">
Line 204: Line 209:
 
</div>
 
</div>
 
<br><br>
 
<br><br>
−
<button data-toggle="collapse" data-target="#mutation">Mutation Primers</button>
+
<button data-toggle="collapse" class="btn-block" data-target="#mutation">Mutation Primers</button>
 
<div id="mutation" class="collapse">
 
<div id="mutation" class="collapse">
 
  <table class="table">
 
  <table class="table">
Line 593: Line 598:
 
<td> araE_BB_rev </td>
 
<td> araE_BB_rev </td>
 
<td> TCCGTATTGATAGTAACCATCTAGTATTTCTCCTCTTTCTCTAGTAGC </td>
 
<td> TCCGTATTGATAGTAACCATCTAGTATTTCTCCTCTTTCTCTAGTAGC </td>
−
</tr>
+
</tr>
 +
<tr>
 +
  <td>Bb_site_fw</td>
 +
  <td>CGCTTCTAGA
 +
GTACTAGTAGCGGCCGCTGCAGGGGTGCTACTTAAGCCTTTAGG</td>
 +
</tr>
 +
<tr>
 +
  <td>Bb_site_rev</td>
 +
  <td >CTACTAGTACTCTAGAAGCGGCCGCGAATTCTCGCAAGCTCGGGCAAATCG</td>
 +
</tr>
 +
<tr>
 +
  <td>Spec_fw</td>
 +
  <td>ACCAATAACTGCCTTAAAAAAACTAATTGAGAGAAGTTTCTATA</td>
 +
</tr>
 +
<tr>
 +
  <td>Spec_rev</td>
 +
 
 +
  <td>GGAGCTAAGGAAGCTAAAATGAGCAATTTGATTAACGGA</td>
 +
</tr>
 +
<tr>
 +
  <td>Backbone_Spec_fw</td> 
 +
  <td>TATAGAAACTTCTCTCAATTAGTTTTTTTAAGGCAGTTATTGGT</td>
 +
</tr>
 +
<tr>
 +
  <td>Backbone_Spec_rev</td>
 +
  <td>TCCGTTAATCAAATTGCTCATTTTAGCTTCCTTAGCTCC</td>
 +
</tr>
 +
<tr>
 +
  <td>Pol_Backb_Fw</td>
 +
 
 +
  <td>CCGTTAATCAAATTGCTCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGAT</td>
 +
</tr>
 +
<tr>
 +
  <td>Pol_Backb_Rev</td>
 +
  <td>TAGAAACTTCTCTCAATTAGTTTTTTTAAGGCAGTTATTGGTGCCCTTAAACG</td>
 +
</tr>
 +
<tr>
 +
  <td>SpecR_Fw</td>
 +
  <td>CAGGAGCTAAGGAAGCTAAAATGAGCAATTTGATTAACGGAAAAATACCAAATCAAG</td>
 +
</tr>
 +
<tr>
 +
    <td>SpecR_Rev</td>
 +
  <td>CAATAACTGCCTTAAAAAAACTAATTGAGAGAAGTTTCTATAGAATTTTTCATATACTTA</td>
 +
</tr>
 +
<tr>
 +
  <td>EP_Cont_Fw</td>
 +
  <td>CGACTACTCGCAGAATGAACTGCG</td>
 +
</tr>
 +
<tr>
 +
  <td>WT_Cont_Fw</td>
 +
 +
  <td>GGACTACTCGCAGATTGAACTGCG</td>
 +
</tr>
 +
<tr>
 +
  <td>eStop_fw</td>
 +
  <td>ACACCAGCGTTTCTGGGTGAGCAAAAAC</td>
 +
</tr>
 +
<tr>
 +
  <td>eStop_rev</td>
 +
  <td>TCACCCAGAAACGCTGGTGTAAGTAAAAGATG</td>
 +
</tr>
 +
<tr>
 +
    <td>lStop_fw</td>
 +
  <td>TATACCGCGCCACATAGCAGAACTTTAAAAGT</td>
 +
</tr>
 +
<tr>
 +
  <td>lStop_rev</td>
 +
  <td>TCTGCTATGTGGCGCGGTATAATCCC</td>
 +
</tr>
 +
<tr>
 +
  <td>Gibs_PolI_fw</td>
 +
  <td>GATTTCTGGAATTCGCGGCCGCTTCTAGAGCAATACGCAAACCGCCTCTCCCC</td>
 +
</tr>
 +
<tr>
 +
  <td>Gibs_PolI_rev</td>
 +
  <td>TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAGTGCGCCTGATCCCAGTTTTCGC</td>
 +
</tr>
 +
<tr>
 +
  <td>Gibs_Pol_BB_fw</td>
 +
  <td>AGTGGCGAAAACTGGGATCAGGCGCACTAATACTAGTAGCGGCCGCTGCAGTC</td>
 +
</tr>
 +
<tr>
 +
  <td>Gibs_Pol_BB_rev</td>
 +
  <td>AACGCGCGGGGAGAGGCGGTTTGCGTATTGCTCTAGAAGCGGCCGCGAATTCCA</td>
 +
</tr>
 
     </tbody>
 
     </tbody>
 
   </table>
 
   </table>
 
</div>
 
</div>
 
<br><br>
 
<br><br>
−
<button data-toggle="collapse" data-target="#selection">Selection Primers</button>
+
<button data-toggle="collapse" class="btn-block" data-target="#selection">Selection Primers</button>
 
<div id="selection" class="collapse">
 
<div id="selection" class="collapse">
 
  <table class="table">
 
  <table class="table">

Latest revision as of 22:30, 19 October 2016



Primers

Primed for success

Primers


Name Sequence (5' → 3')
VF CCACCTGACGTCTAAGAAAC
VR GTATTACCGCCTTTGAGTGAGC


Name Sequence (5' → 3')
Target_bb_fw TACTAGAGAAAAAAAAACCCCGCCCC
Target_bb_rev CAAGTCCTCTTCAGAAATAAGCTTTTGTTC
MB-Insert-fw GAACAAAAGCTTATTTCTGAAGAGGACTTGGTTTCTTCTGTTCCGACCAAACTGG
MB-Insert-rev GTCAGGGGCGGGGTTTTTTTTTCTCTAGTAGGTACGGTAGTTGATAGAGATCGGA
NB-Insert-fw GAACAAAAGCTTATTTCTGAAGAGGACTTGCAGGTTCAGCTGGTTGAATCTGGT
NB-Insert-rev GGGCGGGGTTTTTTTTTCTCTAGTAAGCAGCGCAGTAGTAGATAGCGG
NB-bb-fw GGGTACGCAGGTTACCGTTTCTTCTTACTAGAGAAAAAAAAACCCCGCCCC
NB-bb-rev AGCAGCGCAGTAGTAGATAGCGG
MB-bb-fw TCTCCGATCTCTATCAACTACCGTAC
MB-bb-rev AACGGTAACAGCCGGAGCGTC
NB-Control-fw ACACCGTTACCCTGCAAATGAACAAC
NB-control-rev ACTGCAGCGGCCGCTACTAGTA
NB-psb-I1-fw GGAATTCGCGGCCGCTTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACT
NB-psb-I1-rev GTACCTAGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCAT
NB-psb-I2-fw CCCTGACAGGGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAGTCCGGCAAA
NB-psb-I2-rev GACTGCAGCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCG
MB-psb-1-fw GGAATTCGCGGCCGCTTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTAC
MB-psb-1-rev GTACCTAGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCT
MB-psb-3-fw CTACCCCGACCTCTCTGCTGATCTCTTGGGACGCTCCGGCTGTTACCGTT
MB-psb-3-rev GTAACAGCCGGAGCGTCCCAAGAGATCAGCAGAGAGGTCGGGGTAGCAGCA
MB-psb-4-fw CTCCGATCTCTATCAACTACCGTACCAAAAAAAAACCCCGCCCCTGACAGGGCGGG
MB-psb-4-rev CCTGTCAGGGGCGGGGTTTTTTTTTGGTACGGTAGTTGATAGAGATCGGAGA
BIFC_GFP_contr_fw ATGTCTAAAGGTGAAGAACTGTTCACCG
BIFC_GFP_contr_rev TCCGGAACGTCGTACGGGTACAT
BIFC_BINDER_contr_fw GTTCAGCTGGTTGAATCTGGTGGT
BIFC_BINDER_contr_rev TTCGATGTTGTGACGGATTTTGAAGTTAGC
LIB-bb-fw CTGGAATTCGCGGCCGCTTCTAGAG
LIB-bb-rev CTCTAGAAGCGGCCGCGAATTCCAG
Phag_MB_pAK100blaKDIR_fw CGCTGGATTGTTATTACTCGCGGCCCAGCCGGCCTTTGCTGTTTCTTCTGTTCCGACCAAACTGGAA
Phag_MB_pAK100_rev CTTCTGCTCGAATTCGGCCCCCGAGGCCGAGTAGGTACGGTAGTTGATAGAGATCG
Phag_NB_pAK100blaKDIR _fw CGCTGGATTGTTATTACTCGCGGCCCAGCCGGCCTTTGCTCAGGTTCAGCTGGTTGAATCTGGT
Phag_MB_pAK100_rev CTTCTGCTCGAATTCGGCCCCCGAGGCCGAGTAAGAAGAAACGGTAACCTGCGTAC
Zk_Seq_fw1 CGTGGTGTGAAGCTGAACAAAAGCTT
Zk_Seq_rev1 AATCCCACGCGGTATCGCCCA
Zk_Seq_fw2 TGCATGGCAGCCAGCATAGCG
Zk_Seq_rev2 CATTTCCGCTTCCAGCGCGC
Zk_Insert_pSB1C3_fw GATTTCTGGAATTCGCGGCCGCTTCTAGAGATTAGATGTATCGGCGTAAGTAATCGCG
Zk_Insert_pSB1C3_rev TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAGGCAGAGACCGCAGTTGACAAAAA
Zk_cMyc_BB_fw ATCTTTTTGTCAACTGCGGTCTCTGCCTAATACTAGAGAAAAAAAAACCCCGCCCCT
Zk_cMyc_BB_rev GTCGCGATTACTTACGCCGATACATCTAATCAAGTCCTCTTCAGAAATAAGCTTTTGTTCA


Name Sequence (5' → 3')
emrR_Gib_fw GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCACACAGGAAACCTACTAGATGGATAGTTCGTTTACGCCC
emrR_Gib_rev TAAATTTGTCATCTAGTAGGTTTCCTGTGTGATTATTAGCTCATCGCTTCGAGAAC
cPCR_ugi_cda1 CTGGGGTTACGCGGTTAAC
ugi_Gib_fw GCGATGAGCTAATAATCACACAGGAAACCTACTAGATGACAAATTTATCTGACATCATTGA
ugi_Gib_rev CGCGTCGGTCATCTAGTAGGTTTCCTGTGTGATTATTATAACATTTTAATTTTATTTTCTCCATTACT
ugi-cda1_Gib_fw AAAATGTTATAATAATCACACAGGAAACCTACTAGATGACCGACGCGGAATAC
ugi-cda1_Gib_rev GACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTAAACCGCCGGAGATTTGG
WT_PolI_Gib_for ACCATGATTACGCCAAGCTTATGGTTCAGATCCCCCAAAA
WT_PolI_Gib_rev AACGACGGCCAGTGGAATTCTTAGTGCGCCTGATCCCAGT
pEPPolI_Gib_for GAATTCCACTGGCCGTCGTT
pEPPolI_Gib_rev TTTTGGGGGATCTGAACCATAAGC
pEPPolI_Seq_for GCACCCCAGGCTTTACAC
pEPPolI_Seq_rev GGGTAACGCCAGGGTTTT
pEPPolI_Col_rev GCGGGCGATGTGATTTGT
pSB1K3_Del_for GCTGAGTTGAAGGATCAGCTCAGGAAAGAACATGTGAGCA
pSB1K3_Del_rev TGCTCACATGTTCTTTCCTGAGCTGATCCTTCAACTCAGC
Sbla_T1_Gib_for GGTTCCTGGCCTTTTGCTGGCCTATTTGTTTATTTTTCTA
Sbla_T1_Gib_rev ATCTTCAGCATCTTTTACTTACACCAGCGTTTCTGGGTGA
Sbla_T2_Gib_for TCACCCAGAAACGCTGGTGTAAGTAAAAGATGCTGAAGAT
Sbla_T2_Gib_rev AAGAACATGTGAGCAAAAGGATATGAGTAAACTTGGTCTG
pSB1K3D_Gib_for CAGACCAAGTTTACTCATATCCTTTTGCTCACATGTTCTT
pSB1K3D_Gib_rev TAGAAAAATAAACAAATAGGCCAGCAAAAGGCCAGGAACC
Sbla_EndSeq_rev GCCTGGAGCAAGACGTTT
pSB1K3D_Col_for ACACAACGTGGCTTTGTTG
pSB1K3D_Col_rev GGTTCCTGGCCTTTTGCT
Sbla_Col_for GGTTTCGCCACCTCTGAC
pSB1K3_D_for_P2 TCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTC
pSB1K3_D_rev_P1 GGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGA
RBS_RFP_Ter_for TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG
RBS_RFP_Ter_rev CTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTATTAAGCACCGGTGGAGTGA
C3BB_Ter_for TAGAGAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAGTCCG
C3BB_And_01_rev CTCTTTCTCTAGTAGCTAGCATTGTACCTAGGACTGAGCTAGCCATAAACTCTAGAAGCGGCCGCGAATTCCAGAAATCA
C3BB_And_047_rev CTCTTTCTCTAGTAGCTAGCACTATACCTAGGACTGAGCTAGCCGTAAACTCTAGAAGCGGCCGCGAATTCCAGAAATCA
C3BB_And_086_rev CTCTTTCTCTAGTAGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCA
VF2 TGCCACCTGACGTCTAAGAA
VR ATTACCGCCTTTGAGTGAGC
BFP_Gib_for ACATACTAGAGAAAGAGGAGAAATACTAGATGAGCGAACTGATCAAAGAGAA
BFP_Gib_rev GATCTACACTAGCACTATCAGCGTTATTAATTCAGTTTATGACCCAGCTTG
K3_BFP_Gib_for CAAGCTGGGTCATAAACTGAATTAATAACGCTGATAGTGCTAGTGTAGATC
K3_BFP_Gib_rev TTCTCTTTGATCAGTTCGCTCATCTAGTATTTCTCCTCTTTCTCTAGTATGT
Web_C3BB_for GCCGCTGCAGTCCGGCAAAAAAG
Web_C3BB_rev GCCGCGAATTCCAGAAATCATCCTTAGCG
pSB1K3_D_rev_P2 TGCTCACATGTTCTTTCCTGCTCTGATGTTACATTGCACAAGATA
cPCR_dnaQ_for GGTCACCGATAGCCTTGC
cPCR_ugi-cda1_for CTGGGGTTACGCGGTTAAC
dnaQ_Gib_for GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCACACAGGAAACCTACTAGATGAGCACTGCAATTACACGC
dnaQ_Gib_rev ATTTTTCTTCATCTAGTAGGTTTCCTGTGTGATTATTATGCTCGCCAGAGGCAAC
dam_Gib_for TGGCGAGCATAATAATCACACAGGAAACCTACTAGATGAAGAAAAATCGCGCTTTTTTG
dam_Gib_rev TGGCGAGCATAATAATCACACAGGAAACCTACTAGATGAAGAAAAATCGCGCTTTTTTG
seqA_Gib_for GCGAAAAAATAATAATCACACAGGAAACCTACTAGATGAAAACGATTGAAGTTGATGATG
seqA_Gib_rev TGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAGATAGTTCCGCAAACCTTCTC
cPCR_dam_for GGAAGCAGAGTTGTATCACTTCG
PolI_Gib_for GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGTTCAGATCCCCCAAAATC
PolI_Gib_rev TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAGTGCGCCTGATCCCAG
E0040_A68T_Gib_for GGAGAAATACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAG
E0040_A68T_Gib_rev GTGAAAAGTTCTTCTCCTTTACGCATCTAGTATTTCTCCTCTTTC
E0040_C379A_Gib_for TCAAAGATGACGGGAACTAAAAGACACGTGCTGAAGTCAA
E0040_C379A_Gib_rev TGACTTCAGCACGTGTCTTTTAGTTCCCGTCATCTTTGAAAAATATAG
E0040_A380T_Gib_for CAAAGATGACGGGAACTACTAGACACGTGCTGAAGTCAAG
E0040_A380T_Gib_rev TTGACTTCAGCACGTGTCTAGTAGTTCCCGTCATCTTTGAAAAAT
GFP_MutSeq_for GATGCAACATACGGAAAACTTACC
Pbad_Change_for CCGTCCACCCCCTAATATCCCGACATACCCGTTTTTTGGGCTAACT
Pbad_Change_rev GATATTAGGGGGTGGACGGGGGGGGCGTCAGGTAGGATCCGC
Pbad_Conf_rev GGATATTAGGGGGTGGACGG
dnaQ_Exp_for TCACACAGGAAACCTACTAGATGAGCACTGCAATTACACGC
dnaQ_Exp_rev CTACACTAGCACTATCAGCGTTATGCTCGCCAGAGGCAAC
dnaQ_ExpBB_for GTTGCCTCTGGCGAGCATAACGCTGATAGTGCTAGTGTAGATC
dnaQ_ExpBB_rev CGTGTAATTGCAGTGCTCATCTAGTAGGTTTCCTGTGTGACTC
PolI_Exp_for TCACACAGGAAACCTACTAGATGGTTCAGATCCCCCAAAATC
PolI_Exp_rev CTACACTAGCACTATCAGCGTTAGTGCGCCTGATCCCA
PolI_Exp_rev ACTGGGATCAGGCGCACTAACGCTGATAGTGCTAGTGTAGATC
PolI_ExpBB_rev TTTTGGGGGATCTGAACCATCTAGTAGGTTTCCTGTGTGACTC
PolI_Seq_A ACGCTTATCAATACCATGACG
PolI_Seq_B TGACAACTACGTCACCATCC
PolI_Seq_C CGTTCGAAGTAAAGGTCCATG
SGFP_A_for GGAGAAATACTAGATGCGTTAAGGAGAAGAACTTTTCACTGGAG
SGFP_A_rev GTGAAAAGTTCTTCTCCTTAACGCATCTAGTATTTCTCCTCTTTC
ugi_Single_for TAAAATTAAAATGTTATAATACTAGTAGCGGCCGCT
ugi_Single_rev CGCTACTAGTATTATAACATTTTAATTTTATTTTCTCCATTACTGTC
cda1_Single_for CTTCTAGAGATGACCGACGCGGAATACG
cda1_Single_rev GCGTCGGTCATCTCTAGAAGCGGCCGC
dam_Single_for CCGCGAAAAAATAATACTAGTAGCGGCCGCT
dam_Single_rev CTACTAGTATTATTTTTTCGCGGGTGAAACG
seqA_Single_for GCTTCTAGAGATGAAAACGATTGAAGTTGATGATGAACT
SeqA_Single_for CAATCGTTTTCATCTCTAGAAGCGGCCGC
MP6_ExpBB_for CCAAATCTCCGGCGGTTTAACGCTGATAGTGCTAGTGTAGATC
MP6_Exp_rev CTACACTAGCACTATCAGCGTTAAACCGCCGGAGATTTGGT
B0031_Conf_for TCACACAGGAAACCTACTAGATG
AraProm_VF CGGGACCAAAGCCATGAC
VF2 TGCCACCTGACGTCTAAGAA
VR ATTACCGCCTTTGAGTGAGC
araE_Ins_for AGAAAGAGGAGAAATACTAGATGGTTACTATCAATACGGAATCTGC
araE_Ins_rev GGGTTTTTTTTTCTCTAGTATCAGACGCCGATATTTCTCAAC
araE_BB_for TGAGAAATATCGGCGTCTGATACTAGAGAAAAAAAAACCCCGCC
araE_BB_rev TCCGTATTGATAGTAACCATCTAGTATTTCTCCTCTTTCTCTAGTAGC
Bb_site_fw CGCTTCTAGA GTACTAGTAGCGGCCGCTGCAGGGGTGCTACTTAAGCCTTTAGG
Bb_site_rev CTACTAGTACTCTAGAAGCGGCCGCGAATTCTCGCAAGCTCGGGCAAATCG
Spec_fw ACCAATAACTGCCTTAAAAAAACTAATTGAGAGAAGTTTCTATA
Spec_rev GGAGCTAAGGAAGCTAAAATGAGCAATTTGATTAACGGA
Backbone_Spec_fw TATAGAAACTTCTCTCAATTAGTTTTTTTAAGGCAGTTATTGGT
Backbone_Spec_rev TCCGTTAATCAAATTGCTCATTTTAGCTTCCTTAGCTCC
Pol_Backb_Fw CCGTTAATCAAATTGCTCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGAT
Pol_Backb_Rev TAGAAACTTCTCTCAATTAGTTTTTTTAAGGCAGTTATTGGTGCCCTTAAACG
SpecR_Fw CAGGAGCTAAGGAAGCTAAAATGAGCAATTTGATTAACGGAAAAATACCAAATCAAG
SpecR_Rev CAATAACTGCCTTAAAAAAACTAATTGAGAGAAGTTTCTATAGAATTTTTCATATACTTA
EP_Cont_Fw CGACTACTCGCAGAATGAACTGCG
WT_Cont_Fw GGACTACTCGCAGATTGAACTGCG
eStop_fw ACACCAGCGTTTCTGGGTGAGCAAAAAC
eStop_rev TCACCCAGAAACGCTGGTGTAAGTAAAAGATG
lStop_fw TATACCGCGCCACATAGCAGAACTTTAAAAGT
lStop_rev TCTGCTATGTGGCGCGGTATAATCCC
Gibs_PolI_fw GATTTCTGGAATTCGCGGCCGCTTCTAGAGCAATACGCAAACCGCCTCTCCCC
Gibs_PolI_rev TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAGTGCGCCTGATCCCAGTTTTCGC
Gibs_Pol_BB_fw AGTGGCGAAAACTGGGATCAGGCGCACTAATACTAGTAGCGGCCGCTGCAGTC
Gibs_Pol_BB_rev AACGCGCGGGGAGAGGCGGTTTGCGTATTGCTCTAGAAGCGGCCGCGAATTCCA


Name Sequence (5' → 3')
pSB1C3_BB_fw TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG
pSB1C3_BB_fw_2 TAATACTAGTAGCGGCCGCTGCAGTCCG
pSB1C3_BB_fw_Gal11P GCTGCGCAAAATTCAGGCGCTGTAATACTAGTAGCGGCCGCTGCAGTCCG
pSB1C3_BB_rv CTCTAGAAGCGGCCGCGAATTCCAGAAATC
pSB1C3_BB_rv_Gal11P TTTGCTTTTTACCCTGGAAGAAATACTCATCTCTAGAAGCGGCCGCGAATTCCAGAAATC
Gal4_BB_fw ATTAAAGCGCTGCTGACCGGCCTGTTTTAATACTAGAGAAAAAAAAACCCCGCC
HA4_BB_fw CCGATTAGCATTAACTATCGCACCTGCTAATACTAGAGAAAAAAAAACCCCGCC
Gal4_BB_HA4_rv TACAGCGTCCTGAACAGTTACGCGTGCCATCTAGTATTTCTCCTCTTTCTCTAGTAGC
Gal4_HA4_fw TAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCACGCGTAACTGTTCA
Gal4_rv GTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTAAAACAGGCCGGTCAGCA
HA4_rv GTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTAGCAGGTGCGATAGTTAATGC
Gal11P_BB_fw AAAGCGCTGCGCAAAATTCAGGCGCTGTAATACTAGAGAAAAAAAAACCCCGCC
SH2_BB_fw ACCGTGTATGGCGTGAGCCCGAACTATTAATACTAGAGAAAAAAAAACCCCGCC
Gal11P_SH2_BB_rv TTTGCTTTTTACCCTGGAAGAAATACTCATCTAGTATTTCTCCTCTTTCTCTAGTAGCT
Gal11P_SH2_fw TAGCTACTAGAGAAAGAGGAGAAATACTAGATGAGTATTTCTTCCAGGGTAAAAAG
Gal11P_rv GTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTACAGCGCCTGAATTTTGC
SH2_rv GTCAGGGGCGGGGTTTTTTTTTCTCTAGTATTAATAGTTCGGGCTCACGC
Reporter_fw ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATACAAGAAAGTTTGTTCATTAGGCAC
Reporter_rv GGCGGGGTTTTTTTTTCTCTAGTATTAAGCACCGGTGGAGTGAC
B1006_BB_fw GCTGAAGGTCGTCACTCCACCGGTGCTTAATACTAGAGAAAAAAAAACCCCGCCCCTGA
B1006_BB_rv TGAACAAACTTTCTTGTATTCTAGAAGCGGCCGCGAATTC
B1006_BB_rv2 CTAGAAGCGGCCGCGAATTC
Reporter_SH2_Gal11P_BB_fw CTGACAGGGCGGGGTTTTTTTTTACTAGAGTTTACGGCTAGCTCAGTCCTAGGT
Reporter_SH2_Gal11P_BB_rv GGGTGCCTAATGAACAAACTTTCTTGTATTCTCTAGAAGCGGCCGCGAAT
Reporter_SH2_Gal11P_fw AATACAAGAAAGTTTGTTCATTAGGC
Reporter_SH2_Gal11P_rv CTAGGACTGAGCTAGCCGTAAACTCTAGTAAAAAAAAACCCCGCCCTG
IMP_BB_fw TGCATCACGGGAGATGC
IMP_BB_GCG_fw GCGTGCATCACGGGAGATGC
IMP_BB_rv CATATGTATATCTCCTTCTTAAAGTTAAACAA
IMP_HA4FP_fw TTGTTTAACTTTAAGAAGGAGATATACATATGGCACGCGTAACTGTTCAGG
IMP_HA4FP_rv TAGGGCAACTAGTGCATCTCCCGTGATGCACGCGCAGGTGCGATAGTTAATGCTAAT
IMP_SH2FP_fw TTGTTTAACTTTAAGAAGGAGATATACATATGAGTATTTCTTCCAGGGTAAAAAGC
IMP_SH2FP_rv TAGGGCAACTAGTGCATCTCCCGTGATGCAATAGTTCGGGCTCACGC
IMP_cI_lambda_fw TTGTTTAACTTTAAGAAGGAGATATACATATGAGCACAAAAAAGAAACCATTAACACAAG
IMP_cI_lambda_rv TAGGGCAACTAGTGCATCTCCCGTGATGCAAAACGTCTCTTCAGGCCACTG
CC_BB_FragA_fw TTCACCTGTGGAGCTTTTTAAGTTACTAGTAGCGGCCGCTGC
CC_BB_FragA_rv TGCTGGCAATCATCTGTGTGCTCTAGAAGCGGCCGCGA
CC_BB_FragAB_fw CGAAGATATGGACCAGATGGCGGTACTAGTAGCGGCCGCTGC
CC_BB_FragAB_rv GGGCGACCCGCTTTGTGAACTTAAAAAGCTCCACAGGTGAAGAAAT
CC_BB_FragAB_Reporter_fw ACAGGGCGGGGTTTTTTTTTCACAAAGCGGGTCGCCC
CC_BB_FragAB_Reporter_rv TGCCTAATGAACAAACTTTCTTGTATTACTTAAAAAGCTCCACAGGTGAAGAAATG
CC_FragA_fw TCGCGGCCGCTTCTAGAGCACACAGATGATTGCCAGCA
CC_FragA_rv GCAGCGGCCGCTACTAGTAACTTAAAAAGCTCCACAGGTGAA
CC_BB_FragA_rv TGCTGGCAATCATCTGTGTGCTCTAGAAGCGGCCGCGA
CC_BB_FragAB_fw CGAAGATATGGACCAGATGGCGGTACTAGTAGCGGCCGCTGC
CC_BB_FragAB_rv GGGCGACCCGCTTTGTGAACTTAAAAAGCTCCACAGGTGAAGAAAT
CC_BB_FragAB_Reporter_fw ACAGGGCGGGGTTTTTTTTTCACAAAGCGGGTCGCCC
CC_BB_FragAB_Reporter_rv TGCCTAATGAACAAACTTTCTTGTATTACTTAAAAAGCTCCACAGGTGAAGAAATG
CC_FragA_fw TCGCGGCCGCTTCTAGAGCACACAGATGATTGCCAGCA
CC_FragA_rv GCAGCGGCCGCTACTAGTAACTTAAAAAGCTCCACAGGTGAA
CC_FragB_fw ATTTCTTCACCTGTGGAGCTTTTTAAGTTCACAAAGCGGGTCGCCC
CC_FragB_rv GCAGCGGCCGCTACTAGTACCGCCATCTGGTCCATATCTTCG
CC_Reporter_fw CATTTCTTCACCTGTGGAGCTTTTTAAGTAATACAAGAAAGTTTGTTCATTAGGCA
CC_Reporter_rv GGGCGACCCGCTTTGTGAAAAAAAAACCCCGCCCTGT
CC_FragAB_Reporter_fw CACACAGATGATTGCCAGCA
CC_FragAB_Reporter_rv CCGCCATCTGGTCCATATCTTCG
CI_lambda_part1_fw TTTACTATCACCGCCAGAGGTAACGTACATTAGGCACCCCGGGCTT
CI_lambda_part2_fw AGTGGCCTGAAGAGACGTTTGAACAAAAGCTTATTTCTGAAGAGGA
CI_lambda_part1_rv TGTGTTAATGGTTTCTTTTTTGTGCTCTAGTATTTCTCCTCTTTCTCTAGTAGC
CI_lambda_part2_rv TACGTTACCTCTGGCGGTGATAGTAAACTCTAGAAGCGGCCGCGAA
CI_lambda_Insert_fw GCTACTAGAGAAAGAGGAGAAATACTAGAGCACAAAAAAGAAACCATTAACACA
CI_lambda_Insert_rv TCCTCTTCAGAAATAAGCTTTTGTTCAAACGTCTCTTCAGGCCACT
RpoA_BB_fw GCAAGCATCGCTGACGAGGAACAAAAGCTTATTTCTGAAGAGGA
RpoA_BB_rv TCTGTCACAGAACCCTGCATCTAGTATTTCTCCTCTTTCTCTAGTAGC
RpoA_fw GCTACTAGAGAAAGAGGAGAAATACTAGATGCAGGGTTCTGTGACAGA
RpoA_rv CAAGTCCTCTTCAGAAATAAGCTTTTGTTCCTCGTCAGCGATGCTTGC
Tet_BB_fw TCGACCTAATACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTAT
Tet_BB_rv CGATGAGCGCATTGTTAGATTTCATCTAGTATTTCTCCTCTTTCTCTAGTACGGT
Tet_fw ACCGTACTAGAGAAAGAGGAGAAATACTAGATGAAATCTAACAATGCGCTCATCG
Tet_rv GGAAGCCATCTAGTATTTCTCCTCTTTCTCTAGTATTAGGTCGAGGTGGCCCG
OR2_cI_434_fw CGCTTCTAGAGACAAGATACATTGTATGAAAATACAAGAAAGTTTGTTCATTAGGCACC
OR2_cI_434_rv TTTCTTGTATTTTCATACAATGTATCTTGTCTCTAGAAGCGGCCGCGA
Seq_Reporter_fw AAGGTTTCAAATGGGAACGTG
Seq_cI_434_rv CACCACGAACCAGCTCTAA
Seq_cI_lambda_rv CGGAACAACTCTCATTGCATG
Seq_FragAB_Reporter_fw1 CAGGTTTCTGTTTCACACACC
Seq_FragAB_Reporter_fw2 TCTTCACCTGTGGAGCTTTTT
Seq_FragAB_Reporter_rv1 TTACGCGATAACGGTTGGGA
Seq_FragAB_Reporter_rv2 GCAGGTAGGTTTGAATCAGTTG