Difference between revisions of "Team:Nagahama/Demonstrate your work"

(Blanked the page)
 
(41 intermediate revisions by 5 users not shown)
Line 1: Line 1:
 +
<html>
  
 +
<style>
 +
 +
/********************************* DEFAULT WIKI SETTINGS  ********************************/
 +
 +
#sideMenu, #top_title {display:none;}
 +
#content { padding:0px; width:1000px; margin-top:-7px; margin-left:0px;}
 +
body {background-color:white; }
 +
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 +
 +
/********************************* MENU ********************************/
 +
/* Wrapper for the menu */
 +
.menu_wrapper {
 +
width:150px;
 +
height:100vh;
 +
position:fixed;
 +
padding:0px;
 +
float:left;
 +
background-color:#f2f2f2;
 +
text-align:left;
 +
}
 +
 +
/* styling for the menu items */
 +
.menu_item {
 +
width:100%;
 +
margin:-2px 0px 0px -20px;
 +
padding: 10px 10px; 
 +
border-bottom: 1px solid #d3d3d3;
 +
font-weight:bold;
 +
color:#000000;
 +
cursor: pointer;
 +
}
 +
 +
/* when hovering on a menu item */
 +
.menu_item:hover {
 +
color:#000000;
 +
background-color: #72c9b6;
 +
}
 +
 +
/* decoration icon for the menu buttons*/
 +
.icon {
 +
float:right; 
 +
font-size:16px;
 +
font-weight:bold;
 +
}
 +
 +
/* this is the icon for when the content is collapsed */
 +
.plus::before {
 +
content: "+";
 +
}
 +
/* this is the icon for when the content is expanded */
 +
.less::before {
 +
content: "–";
 +
}
 +
 +
/* styling for the ul that creates the buttons */
 +
ul.menu_items {
 +
width:100%;
 +
margin-left:0px;
 +
padding:0px;
 +
list-style: none;
 +
}
 +
 +
/* styling for the li that are the menu items */
 +
.menu_items li {
 +
width:90%;
 +
margin-top:-2px;
 +
padding: 15px 0px 15px 15px ;
 +
display:block;
 +
border-bottom: 1px solid #d3d3d3; 
 +
text-align:left;
 +
font-weight:bold;
 +
text-decoration:none;
 +
color:#000000;
 +
list-style-type:none;
 +
cursor:pointer;
 +
-webkit-transition: all 0.4s ease;
 +
-moz-transition: all 0.4s ease;
 +
-ms-transition: all 0.4s ease;
 +
-o-transition: all 0.4s ease; transition: all 0.4s ease; 
 +
}
 +
 +
.menu_item a {
 +
width: 100%;
 +
margin-left: -20px;
 +
padding: 11px 90px 12px 20px;
 +
text-decoration: none;
 +
color:black;
 +
}
 +
 +
/* When hovering on a menu item */
 +
.menu_items li:hover {
 +
background-color:#72c9b6;
 +
color: #000000;
 +
}
 +
 +
/* styling for the submenus */
 +
.submenu {
 +
width:100%;
 +
display: none; 
 +
font-weight:bold;
 +
cursor:pointer;
 +
 +
}
 +
 +
/* moving the margin for the submenu ul list */
 +
ul.submenu {
 +
width: 100%;
 +
margin: 10px 0px -11px 0px;
 +
list-style: none;
 +
}
 +
 +
/*styling for the submenu buttons */
 +
.submenu li {
 +
width: 100%;
 +
margin-left: 10px;
 +
margin-bottom: 0px;
 +
}
 +
 +
 +
/* hover state for the submenu buttons */
 +
.submenu li a {
 +
width: 100%;
 +
padding: 5px 10px;
 +
display: inline-block;
 +
border-bottom: 1px solid #d3d3d3;
 +
background-color:white;
 +
text-decoration:none;
 +
color:#000000;
 +
}
 +
 +
 +
 +
.submenu li a:hover  {
 +
background-color:#000000;
 +
color: #72c9b6;
 +
}
 +
 +
 +
/* When the screen is smaller than 680px, the menu has the option to hide/show - this button controls that */
 +
.collapsable_menu_control {
 +
width:100%;
 +
padding: 15px 0px;
 +
display:none;
 +
background-color:#000000;
 +
text-align:center; 
 +
font-weight:bold;
 +
color:#72c9b6;
 +
cursor:pointer;
 +
-webkit-transition: all 0.4s ease;
 +
-moz-transition: all 0.4s ease;
 +
-ms-transition: all 0.4s ease;
 +
-o-transition: all 0.4s ease;
 +
transition: all 0.4s ease;
 +
}
 +
 +
/* when hovering on that button */
 +
.collapsable_menu_control:hover {
 +
background-color: #72c9b6;
 +
color:#000000; 
 +
}
 +
 +
/********************************* CONTENT OF THE PAGE ********************************/
 +
 +
/* Wrapper for the content */
 +
.content_wrapper {
 +
width: 85%;
 +
margin-left:150px;
 +
padding:10px 0px;
 +
float:left;
 +
background-color:white;
 +
}
 +
 +
/*LAYOUT */
 +
.column  {
 +
padding: 10px 0px;
 +
float:left;
 +
background-color:white;
 +
}
 +
 +
.full_size {
 +
width:100%;
 +
}
 +
 +
.full_size img {
 +
padding: 10px 15px;
 +
width:96.5%;
 +
}
 +
 +
.half_size {
 +
width: 50%;
 +
}
 +
.half_size img {
 +
padding: 10px 15px;
 +
width: 93%;
 +
}
 +
 +
 +
.clear {
 +
clear:both;
 +
}
 +
 +
.highlight {
 +
width: 90%;
 +
margin: auto;
 +
padding: 15px 5px;
 +
background-color: #f2f2f2;
 +
}
 +
 +
. {
 +
border: 4px solid #72c9b6;
 +
display: block;
 +
margin: 5px 15px;
 +
width: 95%;
 +
font-weight:bold;
 +
}
 +
 +
 +
/*STYLING */
 +
 +
/* styling for the titles */
 +
.content_wrapper h1, .content_wrapper h2 {
 +
padding:5px 15px;
 +
border-bottom:0px;
 +
color:#72c9b6;
 +
 +
}
 +
.content_wrapper h3, .content_wrapper h4, .content_wrapper h5, .content_wrapper h6 {
 +
padding:5px 15px;
 +
border-bottom:0px;
 +
color: #000000; 
 +
}
 +
 +
 +
/* font and text */
 +
.content_wrapper p {
 +
padding:0px 15px;
 +
font-size: 13px;
 +
font-family:Tahoma, Geneva, sans-serif;
 +
}
 +
 +
/* Links */
 +
.content_wrapper a {
 +
font-weight: bold;
 +
text-decoration: underline;
 +
text-decoration-color:#72c9b6;
 +
color: #72c9b6;
 +
-webkit-transition: all 0.4s ease;
 +
-moz-transition: all 0.4s ease;
 +
-ms-transition: all 0.4s ease;
 +
-o-transition: all 0.4s ease;
 +
transition: all 0.4s ease;
 +
}
 +
 +
/* hover for the links */
 +
.content_wrapper a:hover {
 +
text-decoration:none;
 +
color:#000000;
 +
}
 +
 +
/* non numbered lists */
 +
.content_wrapper ul {
 +
padding:0px 20px;
 +
font-size: 13px;
 +
font-family:Tahoma, Geneva, sans-serif;
 +
}
 +
 +
/* numbered lists */
 +
.content_wrapper ol {
 +
padding:0px;
 +
font-size: 13px;
 +
font-family:Tahoma, Geneva, sans-serif;
 +
}
 +
 +
/* Table */
 +
.content_wrapper table {
 +
width: 97%;
 +
margin:15px 10px;
 +
border: 1px solid #d3d3d3;
 +
border-collapse: collapse;
 +
}
 +
 +
/* table cells */
 +
.content_wrapper  td {
 +
padding: 10px;
 +
vertical-align: text-top;
 +
border: 1px solid #d3d3d3;
 +
border-collapse: collapse;
 +
}
 +
 +
/* table headers */
 +
.content_wrapper th {
 +
padding: 10px;
 +
vertical-align: text-top;
 +
border: 1px solid #d3d3d3;
 +
border-collapse: collapse;
 +
background-color:#f2f2f2;
 +
}
 +
 +
/* Button class */
 +
.button_click {
 +
margin: 10px auto;
 +
padding: 15px; width:12%; 
 +
text-align:center;
 +
font-weight:bold;
 +
background-color: #72c9b6;
 +
cursor:pointer; 
 +
-webkit-transition: all 0.4s ease;
 +
-moz-transition: all 0.4s ease;
 +
-ms-transition: all 0.4s ease;
 +
-o-transition: all 0.4s ease;
 +
transition: all 0.4s ease;
 +
}
 +
 +
/* button class on hover */
 +
.button_click:hover { 
 +
background-color:#000000;
 +
color:#72c9b6;
 +
}
 +
 +
/********************************* RESPONSIVE STYLING ********************************/
 +
 +
/* IF THE SCREEN IS LESS THAN 1000PX */
 +
 +
@media only screen and (max-width: 1000px) {
 +
 +
#content {width:100%; }
 +
.menu_wrapper {width:15%;}
 +
.content_wrapper {margin-left: 15%;}
 +
.menu_item {display:block;}
 +
.icon {display:none;}
 +
.highlight {padding:10px 0px;}
 +
}
 +
 +
 +
/* IF THE SCREEN IS LESS THAN 680PX */
 +
 +
@media only screen and (max-width: 680px) {
 +
.collapsable_menu_control { display:block;}
 +
.menu_item {display:none;}
 +
.menu_wrapper { width:100%; height: 15%; position:relative;}
 +
.content_wrapper {width:100%; margin-left:0px;}
 +
.column.half_size {width:100%; }
 +
.column img { width: 100%; padding: 5px 0px;}
 +
.icon {display:block;}
 +
.highlight {padding:15px 5px;}
 +
}
 +
 +
</style>
 +
 +
 +
 +
 +
<!--- THIS IS WHERE THE HTML BEGINS --->
 +
 +
 +
<!-- This tells the browser that your page is responsive -->
 +
 +
<head>
 +
<meta name="viewport" content="width=device-width, initial-scale=1">
 +
</head>
 +
 +
 +
 +
 +
<div class="menu_wrapper" >
 +
 +
 +
 +
 +
<div class="collapsable_menu_control"> MENU ▤ </div>
 +
 +
<ul id="accordion" class="accordion">
 +
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama">HOME </a> </li>
 +
 +
<li class="menu_item"> <div class="icon plus"></div> PROJECT 
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Introduction">  ・Introduction </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Result and Discussion"> ・Result and Discussion </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Future"> ・Future </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Protocol"> ・Protocol </a></li>
 +
                    </ul>
 +
                </li>
 +
 +
<li class="menu_item"> <div class="icon plus"></div> BioBricks 
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Our all BioBricks">  ・Our all BioBricks </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/BioBricks for medal"> ・BioBricks for medal </a></li>
 +
                    </ul>
 +
                </li>
 +
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama/Safety"> Safety </a> </li>
 +
 +
 +
<li class="menu_item"> <div class="icon plus"></div> Attributions
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Team">  ・Team</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Attributions"> ・Attributions </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Sponsors"> ・Sponsors</a></li>
 +
 +
                    </ul>
 +
                </li>
 +
 +
 +
<li class="menu_item"> <div class="icon plus"></div> Collaborations
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Collaborations">  ・Collaborations</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/The Annual Meeting Of The Genetics Society Of Japan"> ・The Annual Meeting Of The Genetics Society Of Japan </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Support Gifu"> ・Support Gifu</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/virginia">  ・Virginia</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Paris-Saclay">  ・Paris-Saclay</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Munich United"> ・Munich United</a></li>
 +
 +
 +
 +
 +
                    </ul>
 +
                </li>
 +
 +
<li class="menu_item"> <div class="icon plus"></div> Human Practice
 +
<ul class="submenu">
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Human Practice"> ・Human Practice </a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Local"> ・Contacting Local</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Japan"> ・Contacting Japan</a></li>
 +
<li> <a href="https://2016.igem.org/Team:Nagahama/Intgrated Human Practice"> ・Intgrated Human Practice</a></li>
 +
 +
 +
 +
                    </ul>
 +
                </li>
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama/Proof of concept"> Proof of concept </a> </li>
 +
 +
 +
<li class="menu_item"> <a href="https://2016.igem.org/Team:Nagahama/Demonstrate your work"> Demonstrate your work </a> </li>
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
              　　　　　　　</ul>
 +
　　        </li>
 +
 +
 +
</div>
 +
 +
 +
 +
 +
 +
<div class="content_wrapper">
 +
 +
 +
 +
<h1 id="team_name">  </h1>
 +
<h4 id="page_name">  </h4>
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
<script>
 +
 +
// This is the jquery part of your template.  Try not modify any of this code since it makes your menu work.
 +
 +
$(document).ready(function() {
 +
 +
$("#HQ_page").attr('id','');
 +
 +
 +
if ( wgPageName.substring( 0,  8) == "Template")  {  // if the page is a template it displays the full name in a single line
 +
$("#team_name").html( wgPageName );
 +
}
 +
 +
else if (  ( (wgPageName.match(/\//g) || []).length ) == 0 ) {  // if it is the home page , just print the team's name
 +
$("#team_name").html( wgPageName.substring( 5, wgPageName.length ) );
 +
}
 +
 +
else {
 +
// this adds the team's name as an h1
 +
$("#team_name").html( wgPageName.substring( 5 , wgPageName.indexOf("/")  ) );
 +
 +
// this adds the page's title as an h4
 +
$("#page_name").html (    ( wgPageName.substring( wgPageName.indexOf("/") + 1, wgPageName.length ) ).replace( /\/|_/g , " ")  );
 +
}
 +
 +
 +
 +
 +
$('#accordion').find('.menu_item').click(function(){
 +
 +
//Expand or collapse this panel
 +
submenu = $(this).find('.submenu');
 +
submenu.toggle();
 +
 +
icon = $(this).find('.icon');
 +
 +
if ( !$( submenu ).is(':visible') ) {
 +
icon.removeClass("less").addClass("plus");
 +
}
 +
else {
 +
icon.removeClass("plus").addClass("less");
 +
}
 +
 +
//Hide the other panels
 +
$(".submenu").not(submenu).hide();
 +
$(".icon").not(icon).removeClass("less").addClass("plus");
 +
});
 +
 +
 +
$(".collapsable_menu_control").click(function() {
 +
$(".menu_item").toggle();
 +
});
 +
 +
$( window ).resize(function() {
 +
$(".menu_item").show();
 +
});
 +
 +
 +
});
 +
 +
 +
 +
 +
</script>
 +
 +
 +
</html>
 +
We aimed to manufacture Flavorator by using various methods and to commercialize it.
 +
We performed demonstration.
 +
Flavorator is a system to release fragrance which Escherichia coli made farnesol.
 +
Flavorator preserve food by fragrance.
 +
For this reason, Flavorator does not need electricity.<br>
 +
[[File:Fig1- Comparison of the suppressing effect of geraniol, farnesol and ethanol o.png |950px|thumb|center|Fig1- Comparison of the suppressing effect of geraniol, farnesol and ethanol on mold.png
 +
]]
 +
A: geraniol(100 μl of geraniol solution was dropped on the cotton）B:farnesol(100 μl) C: ethanol (100 μl )<br>
 +
A:Chopstick dipped in suspension of mold was touched in the center of the bread. The volume of box is 846cm³.<br>
 +
B:The same treatment was done. C:The same treatment was done. These boxes were kept for 5 days at room temperature.<br>
 +
Farnesol had the highest antifungal activity against mold.
 +
We found that farnesol has high antifungal activity against the mold of bread.
 +
Therefore, we investigated whether farnesol exerts similar antifungal effects on other food.<br>
 +
[[File:ファルネソール パン.png |950px|thumb|center|Fig2:Effects of farnesol on bread mold.]]
 +
A: farnesol(1ml of farnesol solution was dropped on the cotton), B:ddH2O(1ml). <br>
 +
A:Chopstick dipped in suspension of mold was touched in the center of the bread. The volume of box is 846cm³.<br>
 +
B: The same treatment was done. These boxes were kept for 8 days at room temperature.<br>
 +
[[File:ごはん.png |950px|thumb|center|Fig3:Effects of farnesol on rice mold.]]
 +
A: farnesol(1ml of farnesol solution was dropped on the cotton), B:ddH2O(1ml). <br>
 +
A:Chopstick dipped in suspension of mold was touched in the center of the rice. The volume of box is 846cm³.<br>
 +
B: The same treatment was done. These boxes were kept for 8 days at room temperature.<br>
 +
[[File:result_kamadaPOC3.jpg|800px|thumb|center|Fig4:Effects of farnesol on maggot.]]
 +
A: farnesol(1ml of farnesol solution was dropped on the cotton), B:ddH2O(1ml).<br>
 +
A:Chopstick dipped in suspension of rotten meat was touched in the center of the chicken. The volume of box is 846cm³.<br>
 +
B: The same treatment was done. These boxes were kept for 8 days at room temperature.<br>
 +
We found that farnesol has a preservative effect on various foods.
 +
So, we think about commercialization of "Flavorator" in the future.<br>
 +
[[File:香蔵庫２.jpeg|800px|thumb|center|Fig5. Model diagram of Flavorator]]
 +
[[File:香蔵庫 中.jpeg|800px|thumb|center|Fig6. Model diagram 2 of Flavorator]]
 +
①Door of place where preserve food ②Door of place where put Escherichia coli　③Culture medium of E. coli ④filter　⑤wire screen　⑥food<br>
 +
How to replace Escherichia coli: Replacement<br>
 +
Door: A place to put food, and a place to put Escherichia coli<br>
 +
We lowered height of Flavorator.
 +
As a result, Farnesol became easy to reach to food．<br>
 +
Refrigerator consumes the power of 300 kWh per year.
 +
Carbon dioxide emissions of a refrigerator’s in one year is 134.1kg.
 +
It is possible to reduce emissions of carbon dioxide and consumption of electricity by changing to Flavorator from the refrigerator.<br>
 +
For this reason, it will prevent global warming.<br>
 +
From here, we will assume that Flavorator was completed.
 +
We think that the next problem is how to commercialize Flavorator.
 +
We can make Escherichia coli synthesize farnesol.
 +
However, in order to commercialize the Flavorator we need cooperation of people from other fields.
 +
For example, system to circulate the fragrance requires cooperation from Food preservation fields.
 +
We continue to overcome these problem, we would like to commercialize Flavorator.

Latest revision as of 22:55, 19 October 2016

MENU ▤
　　

We aimed to manufacture Flavorator by using various methods and to commercialize it. We performed demonstration. Flavorator is a system to release fragrance which Escherichia coli made farnesol. Flavorator preserve food by fragrance. For this reason, Flavorator does not need electricity.

Fig1- Comparison of the suppressing effect of geraniol, farnesol and ethanol on mold.png

A: geraniol(100 μl of geraniol solution was dropped on the cotton）B:farnesol(100 μl) C: ethanol (100 μl )
A:Chopstick dipped in suspension of mold was touched in the center of the bread. The volume of box is 846cm³.
B:The same treatment was done. C:The same treatment was done. These boxes were kept for 5 days at room temperature.
Farnesol had the highest antifungal activity against mold. We found that farnesol has high antifungal activity against the mold of bread. Therefore, we investigated whether farnesol exerts similar antifungal effects on other food.

Fig2:Effects of farnesol on bread mold.

A: farnesol(1ml of farnesol solution was dropped on the cotton), B:ddH2O(1ml).
A:Chopstick dipped in suspension of mold was touched in the center of the bread. The volume of box is 846cm³.
B: The same treatment was done. These boxes were kept for 8 days at room temperature.

Fig3:Effects of farnesol on rice mold.

A: farnesol(1ml of farnesol solution was dropped on the cotton), B:ddH2O(1ml).
A:Chopstick dipped in suspension of mold was touched in the center of the rice. The volume of box is 846cm³.
B: The same treatment was done. These boxes were kept for 8 days at room temperature.

Fig4:Effects of farnesol on maggot.

A: farnesol(1ml of farnesol solution was dropped on the cotton), B:ddH2O(1ml).
A:Chopstick dipped in suspension of rotten meat was touched in the center of the chicken. The volume of box is 846cm³.
B: The same treatment was done. These boxes were kept for 8 days at room temperature.
We found that farnesol has a preservative effect on various foods. So, we think about commercialization of "Flavorator" in the future.

Fig5. Model diagram of Flavorator
Fig6. Model diagram 2 of Flavorator

①Door of place where preserve food ②Door of place where put Escherichia coli　③Culture medium of E. coli ④filter　⑤wire screen　⑥food
How to replace Escherichia coli: Replacement
Door: A place to put food, and a place to put Escherichia coli
We lowered height of Flavorator. As a result, Farnesol became easy to reach to food．
Refrigerator consumes the power of 300 kWh per year. Carbon dioxide emissions of a refrigerator’s in one year is 134.1kg. It is possible to reduce emissions of carbon dioxide and consumption of electricity by changing to Flavorator from the refrigerator.
For this reason, it will prevent global warming.
From here, we will assume that Flavorator was completed. We think that the next problem is how to commercialize Flavorator. We can make Escherichia coli synthesize farnesol. However, in order to commercialize the Flavorator we need cooperation of people from other fields. For example, system to circulate the fragrance requires cooperation from Food preservation fields. We continue to overcome these problem, we would like to commercialize Flavorator.