Difference between revisions of "Team:Nagahama/Demonstrate your work"

 
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<li class="menu_item"> <div class="icon plus"></div> PROJECT   
 
<li class="menu_item"> <div class="icon plus"></div> PROJECT   
 
<ul class="submenu">
 
<ul class="submenu">
<li> <a href="https://2016.igem.org/Team:Nagahama/Introduction">  Introduction </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Introduction">  ・Introduction </a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/Result and Discussion"> Result and Discussion </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Result and Discussion"> ・Result and Discussion </a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/Future"> Future </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Future"> ・Future </a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/Protocol"> Protocol </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Protocol"> ・Protocol </a></li>
 
                     </ul>
 
                     </ul>
 
                 </li>
 
                 </li>
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<li class="menu_item"> <div class="icon plus"></div> BioBricks   
 
<li class="menu_item"> <div class="icon plus"></div> BioBricks   
 
<ul class="submenu">
 
<ul class="submenu">
<li> <a href="https://2016.igem.org/Team:Nagahama/Our all BioBricks">  Our all BioBricks </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Our all BioBricks">  ・Our all BioBricks </a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/BioBricks for medal"> BioBricks for medal </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/BioBricks for medal"> ・BioBricks for medal </a></li>
 
                     </ul>
 
                     </ul>
 
                 </li>
 
                 </li>
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<li class="menu_item"> <div class="icon plus"></div> Attributions
 
<li class="menu_item"> <div class="icon plus"></div> Attributions
 
<ul class="submenu">
 
<ul class="submenu">
<li> <a href="https://2016.igem.org/Team:Nagahama/Team">  Team</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Team">  ・Team</a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/Attributions"> Attributions </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Attributions"> ・Attributions </a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/Sponsors"> Sponsors</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Sponsors"> ・Sponsors</a></li>
  
 
                     </ul>
 
                     </ul>
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<li class="menu_item"> <div class="icon plus"></div> Collaborations
 
<li class="menu_item"> <div class="icon plus"></div> Collaborations
 
<ul class="submenu">
 
<ul class="submenu">
<li> <a href="https://2016.igem.org/Team:Nagahama/Collaborations">  Collaborations</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Collaborations">  ・Collaborations</a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/The Annual Meeting Of The Genetics Society Of Japan"> The Annual Meeting Of The Genetics Society Of Japan </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/The Annual Meeting Of The Genetics Society Of Japan"> ・The Annual Meeting Of The Genetics Society Of Japan </a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/Support Gifu"> Support Gifu</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Support Gifu"> ・Support Gifu</a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/virginia">  Virginia</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/virginia">  ・Virginia</a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/Paris-Saclay">  Paris-Saclay</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Paris-Saclay">  ・Paris-Saclay</a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/Munich United"> Munich United</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Munich United"> ・Munich United</a></li>
  
  
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<li class="menu_item"> <div class="icon plus"></div> Human Practice
 
<li class="menu_item"> <div class="icon plus"></div> Human Practice
 
<ul class="submenu">
 
<ul class="submenu">
<li> <a href="https://2016.igem.org/Team:Nagahama/Human Practice"> Human Practice </a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Human Practice"> ・Human Practice </a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Local"> Contacting Local</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Local"> ・Contacting Local</a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Japan"> Contacting Japan</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Contacting Japan"> ・Contacting Japan</a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/Intgrated Human Practice"> Intgrated Human Practice</a></li>
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<li> <a href="https://2016.igem.org/Team:Nagahama/Intgrated Human Practice"> ・Intgrated Human Practice</a></li>
<li> <a href="https://2016.igem.org/Team:Nagahama/Engagement"> Engagement</a></li>
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Nagahama Rotary Club<br>
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We aimed to manufacture Flavorator by using various methods and to commercialize it.
"Nagahama Rotary Club" is Rotary Club that is active in Nagahama.
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We performed demonstration.
Rotary Club is an international community service federation.
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Flavorator is a system to release fragrance which Escherichia coli made farnesol.
The most important activities of Rotary Club is to enhances its professional members’ ethics and to contribute to society.
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Flavorator preserve food by fragrance.
Leaders in variety occupations or are Rotary club members participate in Rotary Club actuation.
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For this reason, Flavorator does not need electricity.<br>
We have explained synthetic biology and our project to the leaders of various occupations in Nagahama.
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[[File:Fig1- Comparison of the suppressing effect of geraniol, farnesol and ethanol o.png |950px|thumb|center|Fig1- Comparison of the suppressing effect of geraniol, farnesol and ethanol on mold.png
This Activity is two advantages.<br>
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]]
Fig1.Symbol of Rotary Club〈br/〉
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A: geraniol(100 μl of geraniol solution was dropped on the cotton)B:farnesol(100 μl) C: ethanol (100 μl )<br>
Fig2. Banner of Nagahama Rotary Club〈br/〉
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A:Chopstick dipped in suspension of mold was touched in the center of the bread. The volume of box is 846cm³.<br>
1:We spread synthetic biology to the local community of Nagahama.
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B:The same treatment was done. C:The same treatment was done. These boxes were kept for 5 days at room temperature.<br>
This will lead to development of make new specialty products using synthetic biology.〈br/〉
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Farnesol had the highest antifungal activity against mold.
2:We succeed in making a "Flavorator", it has a potential to become a specialty product of Nagahama.〈br/〉
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We found that farnesol has high antifungal activity against the mold of bread.  
We are got two achievements by this activity.
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Therefore, we investigated whether farnesol exerts similar antifungal effects on other food.<br>
The first achievement was spread synthetic biology to Nagahama.
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[[File:ファルネソール パン.png |950px|thumb|center|Fig2:Effects of farnesol on bread mold.]]
And we tell the explained the merits of "Flavorator", it will be easier to commercialize.〈br/〉
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A: farnesol(1ml of farnesol solution was dropped on the cotton), B:ddH2O(1ml). <br>
Fig3. The president of Nagahama Institute of Bio-Science and Technology giving a speech〈br/〉
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A:Chopstick dipped in suspension of mold was touched in the center of the bread. The volume of box is 846cm³.<br>
Fig4. Our team leader announcement of〈br/〉
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B: The same treatment was done. These boxes were kept for 8 days at room temperature.<br>
Lake Biwa Environmental Business Messe〈br/〉
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[[File:ごはん.png |950px|thumb|center|Fig3:Effects of farnesol on rice mold.]]
https://www.biwako-messe.com/〈br/〉
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A: farnesol(1ml of farnesol solution was dropped on the cotton), B:ddH2O(1ml). <br>
Lake Biwa Environmental Business Messe is a comprehensive trade fair of environmental industry.  
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A:Chopstick dipped in suspension of mold was touched in the center of the rice. The volume of box is 846cm³.<br>
Campanies and organizations that are involved in environmental business have exhibited environment products, services, business models and technologies.  
+
B: The same treatment was done. These boxes were kept for 8 days at room temperature.<br>
The purpose of the fair is market development and expansion, and productive business negotiations and transactions are made generally.
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[[File:result_kamadaPOC3.jpg|800px|thumb|center|Fig4:Effects of farnesol on maggot.]]
We talked with some companies and explanined syuthetic biology and our project.
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A: farnesol(1ml of farnesol solution was dropped on the cotton), B:ddH2O(1ml).<br>
Biwako Broadcasting (NHK)〈br/〉
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A:Chopstick dipped in suspension of rotten meat was touched in the center of the chicken. The volume of box is 846cm³.<br>
Biwako Broadcasting is an independent broadcaster.
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B: The same treatment was done. These boxes were kept for 8 days at room temperature.<br>
Its target area is Shiga Prefecture.
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We found that farnesol has a preservative effect on various foods.
Biwako Broadcasting broadcasted a presentation we made in the Lake Biwa Environmental Business Messe.〈br/〉
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So, we think about commercialization of "Flavorator" in the future.<br>
 +
[[File:香蔵庫2.jpeg|800px|thumb|center|Fig5. Model diagram of Flavorator]]
 +
[[File:香蔵庫 中.jpeg|800px|thumb|center|Fig6. Model diagram 2 of Flavorator]]
 +
①Door of place where preserve food ②Door of place where put Escherichia coli ③Culture medium of E. coli ④filter ⑤wire screen ⑥food<br>
 +
How to replace Escherichia coli: Replacement<br>
 +
Door: A place to put food, and a place to put Escherichia coli<br>
 +
We lowered height of Flavorator.
 +
As a result, Farnesol became easy to reach to food.<br>
 +
Refrigerator consumes the power of 300 kWh per year.
 +
Carbon dioxide emissions of a refrigerator’s in one year is 134.1kg.
 +
It is possible to reduce emissions of carbon dioxide and consumption of electricity by changing to Flavorator from the refrigerator.<br>
 +
For this reason, it will prevent global warming.<br>
 +
From here, we will assume that Flavorator was completed.
 +
We think that the next problem is how to commercialize Flavorator.
 +
We can make Escherichia coli synthesize farnesol.
 +
However, in order to commercialize the Flavorator we need cooperation of people from other fields.
 +
For example, system to circulate the fragrance requires cooperation from Food preservation fields.
 +
We continue to overcome these problem, we would like to commercialize Flavorator.

Latest revision as of 22:55, 19 October 2016

We aimed to manufacture Flavorator by using various methods and to commercialize it. We performed demonstration. Flavorator is a system to release fragrance which Escherichia coli made farnesol. Flavorator preserve food by fragrance. For this reason, Flavorator does not need electricity.

Fig1- Comparison of the suppressing effect of geraniol, farnesol and ethanol on mold.png

A: geraniol(100 μl of geraniol solution was dropped on the cotton)B:farnesol(100 μl) C: ethanol (100 μl )
A:Chopstick dipped in suspension of mold was touched in the center of the bread. The volume of box is 846cm³.
B:The same treatment was done. C:The same treatment was done. These boxes were kept for 5 days at room temperature.
Farnesol had the highest antifungal activity against mold. We found that farnesol has high antifungal activity against the mold of bread. Therefore, we investigated whether farnesol exerts similar antifungal effects on other food.

Fig2:Effects of farnesol on bread mold.

A: farnesol(1ml of farnesol solution was dropped on the cotton), B:ddH2O(1ml).
A:Chopstick dipped in suspension of mold was touched in the center of the bread. The volume of box is 846cm³.
B: The same treatment was done. These boxes were kept for 8 days at room temperature.

Fig3:Effects of farnesol on rice mold.

A: farnesol(1ml of farnesol solution was dropped on the cotton), B:ddH2O(1ml).
A:Chopstick dipped in suspension of mold was touched in the center of the rice. The volume of box is 846cm³.
B: The same treatment was done. These boxes were kept for 8 days at room temperature.

Fig4:Effects of farnesol on maggot.

A: farnesol(1ml of farnesol solution was dropped on the cotton), B:ddH2O(1ml).
A:Chopstick dipped in suspension of rotten meat was touched in the center of the chicken. The volume of box is 846cm³.
B: The same treatment was done. These boxes were kept for 8 days at room temperature.
We found that farnesol has a preservative effect on various foods. So, we think about commercialization of "Flavorator" in the future.

Fig5. Model diagram of Flavorator
Fig6. Model diagram 2 of Flavorator

①Door of place where preserve food ②Door of place where put Escherichia coli ③Culture medium of E. coli ④filter ⑤wire screen ⑥food
How to replace Escherichia coli: Replacement
Door: A place to put food, and a place to put Escherichia coli
We lowered height of Flavorator. As a result, Farnesol became easy to reach to food.
Refrigerator consumes the power of 300 kWh per year. Carbon dioxide emissions of a refrigerator’s in one year is 134.1kg. It is possible to reduce emissions of carbon dioxide and consumption of electricity by changing to Flavorator from the refrigerator.
For this reason, it will prevent global warming.
From here, we will assume that Flavorator was completed. We think that the next problem is how to commercialize Flavorator. We can make Escherichia coli synthesize farnesol. However, in order to commercialize the Flavorator we need cooperation of people from other fields. For example, system to circulate the fragrance requires cooperation from Food preservation fields. We continue to overcome these problem, we would like to commercialize Flavorator.