Difference between revisions of "Team:UGent Belgium/Notebook"

 
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<h2>Notebook</h2>
+
<h1>General notebook</h1><br>
  
 
<div class="panel-group">
 
<div class="panel-group">
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 +
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 +
    <div class="panel-heading">
 +
      <h4 class="panel-title">
 +
        <a data-toggle="collapse" href="#February">February</a>
 +
      </h4>
 +
    </div>
 +
    <div id="February" class="panel-collapse  collapse">
 +
      <ul class="list-group">
 +
        <li class="list-group-item borderless"><b>February 29:</b> <ul><li>Our application for iGEM is sent.</li>
 +
          <li>A team of four master students (Bram Danneels, Griet De Clercq, Wouter Steyaert and Chari Vandenbussche) is assembled under guidance of PI Wim.</li></ul></li>         
 +
      </ul>
 +
    </div>
 +
  </div>
 +
 
   <div class="panel">
 
   <div class="panel">
 
     <div class="panel-heading">
 
     <div class="panel-heading">
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     <div id="March" class="panel-collapse  collapse">
 
     <div id="March" class="panel-collapse  collapse">
 
       <ul class="list-group">
 
       <ul class="list-group">
−
         <li class="list-group-item">Week 1: 01/03 - 06/03</li>
+
         <li class="list-group-item borderless"><b>March 03:</b> We start brainstorming for subjects.</li> 
 +
        <li class="list-group-item borderless"><b>March 24:</b> PI's Marjan and Yves join the team.</li>      
 
       </ul>
 
       </ul>
 
     </div>
 
     </div>
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     </div>
 
     </div>
 
     <div id="April" class="panel-collapse  collapse">
 
     <div id="April" class="panel-collapse  collapse">
 +
      <ul class="list-group">
 +
        <li class="list-group-item borderless"><b>April 22:</b> Three subjects are chosen, and we start preparing a presentaion about them.</li>
 +
      </ul> 
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div id="May" class="panel-collapse  collapse">
 
     <div id="May" class="panel-collapse  collapse">
 +
      <ul class="list-group">
 +
        <li class="list-group-item borderless"><b>May 03:</b> Our iGEM application is officially accepted.</li>
 +
        <li class="list-group-item borderless"><b>May 04:</b> <a href="https://static.igem.org/mediawiki/2016/e/ed/T--UGent_Belgium--databanken.pdf" target="_blank">Presentation</a> of our possible subjects for PI Wim and our fellow sudents.</li>
 +
        <li class="list-group-item borderless"><b>May 06:</b> Our team grows, with six phd-students and one adivsor joining.</li>
 +
        <li class="list-group-item borderless"><b>May 17:</b> We sit together for the first time and discuss the different subjects.</li>
 +
        <li class="list-group-item borderless"><b>May 31:</b> We chose our subject! The work gets divided into four work packages, and we divide the work.</li>
 +
        </li>
 +
      </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div id="June" class="panel-collapse  collapse">
 
     <div id="June" class="panel-collapse  collapse">
 +
      <ul class="list-group">
 +
        <li class="list-group-item borderless"><b>June 15:</b> <a href="http://www.ugent.be/p3lab/en/fablab" target="_blank">Fablab</a> was contacted to be a partner for our 3D-printing needs, and they gladly accepted.</li>
 +
        <li class="list-group-item borderless"><b>June 29:</b> We write an abstract on our project, and submit it to our iGEM wiki.</li>
 +
      </ul> 
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div id="July" class="panel-collapse  collapse">
 
     <div id="July" class="panel-collapse  collapse">
 +
      <ul class="list-group">
 +
        <li class="list-group-item borderless"><b>July 07:</b>
 +
          <ul>
 +
            <li>Our <a style="margin-right: 0px; padding-right: 0px;" href="https://www.facebook.com/IGemUgent/" target="_blank">Facebook</a> and <a style="margin-right: 0px; padding-right: 0px;" href="https://twitter.com/igem_ghent" target="_blank">Twitter</a> pages are live.
 +
            </li>
 +
            <li> A first draft of a 'sponsor letter' is made.</li>
 +
          </ul>
 +
        <li class="list-group-item borderless"><b>July 18:</b> We booked our stay in Boston.
 +
        </li>
 +
        <li class="list-group-item borderless"><b>July 19:</b> The 'shape' work package decides to use Autodesk Fusion 360 to shape the water collector.
 +
        </li>
 +
        <li class="list-group-item borderless"><b>July 22:</b> Prof Dan Fernandez (CSUMB), who performs research on fog catchers, is interested to  with us.</li>
 +
      </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div id="August" class="panel-collapse  collapse">
 
     <div id="August" class="panel-collapse  collapse">
 +
      <ul class="list-group">
 +
          <li class="list-group-item borderless"><b>August 01:</b> Our first 3D model of the water collector prototype is ready and printed: a dome with a gutter to collect the water.
 +
<div class="center"><img src="https://static.igem.org/mediawiki/2016/0/0e/First_shape.png" alt="ShapeV1" style="width: 50%"></div>
 +
<div class="center"><img src="https://static.igem.org/mediawiki/2016/b/be/First_shape_print.jpg" alt="ShapeV1Print" height="244" width="326"></div>
 +
</li>
 +
        <li class="list-group-item borderless"><b>August 04:</b> We optimized our water collector shape with holes to distribute the water.
 +
<div class="center"><img src="https://static.igem.org/mediawiki/2016/0/0e/Shape_water_holes.png" alt="ShapeV2" style="width: 50%"></div>
 +
</li>
 +
        <li class="list-group-item borderless"><b>August 15:</b> We created an informational video, showing what our project is about.
 +
          <div class="center"><video controls="" style="width: 500px; height:auto;object-fit: contain;">
 +
<source src="https://static.igem.org/mediawiki/2016/d/d8/T--UGent_Belgium--promovideo.mp4" type="video/mp4" >
 +
</video></div>
 +
        </li>
 +
 +
        <li class="list-group-item borderless"><b>August 19:</b> We successfully dissolved 0.55g PLA in 8ml DMF at 80-90 °C (68.75 g/L) and precipitated it by pouring the solution into room temperature EtOH (disolol, denatured).
 +
        </li>
 +
 +
        <li class="list-group-item borderless"><b>August 20:</b> The filament was precipitated and washed with ethanol (cf. 19/09).
 +
        </li>
 +
 +
        <li class="list-group-item borderless"><b>August 22:</b> We brainstormed about the possibility to stack our collector, but unfortunatly this is not geometrically feasible.
 +
<div class="center"><img src="https://static.igem.org/mediawiki/2016/1/11/Brainstormstacking.png" alt="ShapeV2" height="567" width="745"></div>
 +
</li>
 +
        <li class="list-group-item borderless"><b>August 30:</b> We brainstorm on other ways to combine our collectors. Meanwhile, we redsign the collector with only holes in the bottom ridge, and on the top. Also, the inside of the collector is made hollow.</li>
 +
      </ul> 
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div id="September" class="panel-collapse  collapse">
 
     <div id="September" class="panel-collapse  collapse">
 +
      <ul class="list-group">
 +
        <li class="list-group-item borderless"><b>September 01:</b>
 +
          <ul>
 +
            <li>The first batch of biotin is delivered: the work on the filament can begin!
 +
          <div class="center"><img src="https://static.igem.org/mediawiki/2016/2/25/T--UGent_Belgium--biotin.jpg" alt="Biotin" height="400" width="300"></div></li><br>
 +
            <li>
 +
              Steven, our advisor, met up with the <a href="https://2016.igem.org/Team:EPFL" target="_blank">EPFL team</a> in Lausanne, Switzerland. They immediately bonded over a barbecue.
 +
          <div class="center"><img src="https://static.igem.org/mediawiki/2016/b/bf/T--UGent_Belgium--steven_lausanne.jpg" alt="Steven with the Lausanne team" height="375" width="500"></div>
 +
            </li>
 +
          </ul>
 +
        </li>
 +
        <li class="list-group-item borderless"><b>September 12:</b> We performed some solubility and precipitation tests on the biotin and determine the optimal precipitation solution composition.
 +
          <div class="center"><video controls="" style="width: 500px; height:auto;object-fit: contain;">
 +
<source src="https://static.igem.org/mediawiki/2016/d/d1/T--UGent_Belgium--pla_firstBatch.mp4" type="video/mp4" >
 +
</video></div>
 +
        </li>
 +
 +
 
 +
<li class="list-group-item borderless"><b>September 13:</b>
 +
          <ul>
 +
            <li>We all got to know the <a href="https://2016.igem.org/Team:EPFL" target="_blank">EPFL team</a> better, as they conducted a Skype <a href="http://igem.today/2016/09/21/team-of-the-week-igem-ugent-belgium-beetle-idea/" target="_blank">interview</a> with us.
 +
              <div class="center"><img src="https://static.igem.org/mediawiki/2016/d/d6/T--UGent_Belgium--skype_lausanne.jpg" alt="Skype interview Lausanne" height="255" width="400"></div>
 +
            </li>
 +
            <li>Dewpal has been chosen as name!</li>
 +
            <li>We came up with yet another design, now with internal water distribution.
 +
</li>
 +
          </ul>
 +
        </li>
 +
 +
        <li class="list-group-item borderless"><b>September 14:</b>
 +
          <ul>
 +
            <li>We printed three different prototypes of our water collector.
 +
              <div class="center"><video controls="" style="width: 500px; height:auto;object-fit: contain;">
 +
<source src="https://static.igem.org/mediawiki/2016/8/85/T--UGent_Belgium--3Dprint.mp4" type="video/mp4" >
 +
</video></div>
 +
            </li>
 +
            <li>We scaled the filament up 10 times. A slight raise in temperature was necessary to dissolve the PLA (145°C instead of 130°C on plate). When precipitating in ethanol, a rather glutinous substance was received on top of some big flakes that could be recovered.</li>
 +
          </ul>   
 +
        </li>
 +
 +
        <li class="list-group-item borderless"><b>September 15:</b>
 +
          <ul>
 +
            <li>Our <a style="margin-right: 0px; padding-right: 0px;" href="https://www.gofundme.com/2p5xfhg" target="_blank">crowdfunding page</a> is live!
 +
            </li>
 +
            <li>
 +
            The filament sample (cf. 14/09) was decanted and centrifuged. PLA-biotin was harvested (pellet) and set to dry at 70°C overnight.
 +
            </li>
 +
          </ul>
 +
        </li>
 +
 +
 +
        <li class="list-group-item borderless"><b>September 18:</b>  We made a temperature and humidity sensor with Raspberry Pi so we can observe the environment while testing our 3D shape.
 +
              <div class="center"><img src="https://static.igem.org/mediawiki/2016/f/f1/T--UGent_Belgium--humTempSensor.jpg" alt="Humidity Sensor" height="300" width="400"></div>
 +
        </li>
 +
        <li class="list-group-item borderless"><b>September 20:</b>  The water collector without biotin was tested for its water collecting efficiency.
 +
        </li>
 +
        <li class="list-group-item borderless"><b>September 21:</b>  We met with Winnie from <a href"http://reagentlab.org/" target="_blank">ReaGent</a>.
 +
        </li>
 +
        <li class="list-group-item borderless"><b>September 23:</b> 
 +
          <ul>
 +
            <li>We met with Deepak from <a href="http://www.3dee.be/" target="_blank">3dee</a>, who gave us some amazing advice on how to enhance our 3D shape.</li>
 +
            <li>Our filament group went to <a href="http://www.timelab.org/" target="_blank">Timelab</a> to make a batch of filament using their extruder.
 +
              <div class="center"><img src="https://static.igem.org/mediawiki/2016/9/9d/T--UGent_Belgium--filament_timelab.jpg" alt="Making filament at Timelab" height="400" width="400"></div>
 +
            </li>
 +
          </ul>
 +
        </li>
 +
 +
        <li class="list-group-item borderless"><b>September 24:</b> We made an experimental set-up with a humidifier and temperature/humidity sensor (cf. 15/09) to test the water collection of our shapes.
 +
              <div class="center"><img src="https://static.igem.org/mediawiki/2016/8/88/TT-UGent_Belgium--humidistat.jpg" alt="Humidifier set up" height="300" width="400"></div>
 +
        </li>
 +
 +
        <li class="list-group-item borderless"><b>September 26:</b> We tried our INP, and it works!
 +
              <div class="center"><video controls="" style="width: 500px; height:auto;object-fit: contain;">
 +
<source src="https://static.igem.org/mediawiki/2016/3/3e/T--UGent_Belgium--biofunction_control_inaZ_editedLogo.mp4" type="video/mp4" >
 +
</video></div>
 +
        </li>
 +
 +
        <li class="list-group-item borderless"><b>September 29:</b> Our logo was designed. Our shape design now includes a screw cap to fit the collectors on each other, and on a plastic bottle.
 +
        </li>
 +
      </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div id="October" class="panel-collapse  collapse">
 
     <div id="October" class="panel-collapse  collapse">
 +
      <ul class="list-group">
 +
        <li class="list-group-item borderless"><b>October 01:</b> We improved the design of our shape again, now with an active surface area that is almost 20 times larger than the previous version.
 +
              <div class="center"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--UGent_Belgium--collectorV4.png" alt="Water collector version 4" height="320" width="400"></div>
 +
        </li>
 +
 +
        <li class="list-group-item borderless"><b>October 02:</b>  Yet another change in our design: now with weaving ribbons.
 +
<div class="center"><img src="https://static.igem.org/mediawiki/2016/4/43/Ribbons.png" alt="Water collector version 4" height="289" width="392"></div>
 +
</li>
 +
 +
        <li class="list-group-item borderless"><b>October 07:</b> 3Ders published a <a href="http://www.3ders.org/articles/20161007-ugent-fights-water-scarcity-with-3d-printed-dewpal-water-collector.html" target="_blank">press article</a> on our project.</li>
 +
 +
        <li class="list-group-item borderless"><b>October 09:</b> We started coating the water collector with biotin. Solvent with PLA and biotin is poured over the water collector and left to dry under the laminar air cabinet.
 +
<div class="center"><img src="https://static.igem.org/mediawiki/2016/3/35/Binding_biotine.jpg" alt="Binding biotin with out water collectors" height="384" width="512"></div>
 +
<div class="center"><img src="https://static.igem.org/mediawiki/2016/a/a8/Coating_with_biotin.jpeg" alt="Water collector version 4" height="384" width="512"></div>
 +
</li>
 +
 +
        <li class="list-group-item borderless"><b>October 10:</b> Digital Trends published a <a href="http://www.digitaltrends.com/cool-tech/dewpal-watter-collector/" target="_blank">press article</a> about our project.</li>
 +
 +
    <li class="list-group-item borderless"><b>October 12:</b> Testing four water collectors with four treatments: INP + INP_NC-mSA2, RFP + INP_NC-mSA2, INP_RC-mSA2 and mGFPuv2-mSA2. Each collector is sawn in half, one half receives a treatment and the other half is the control. The water collectors are placed overnight in the controlled humidified chamber.
 +
<div class="center"><img src="https://static.igem.org/mediawiki/2016/9/9c/T--UGent_Belgium--humchamb.jpeg" alt="Binding biotin with out water collectors"  width="600"></div>
 +
</li>
 +
        <li class="list-group-item borderless"><b>October 13:</b> Another <a href="http://www.madeinoostvlaanderen.be/nieuws/gentse-studenten-halen-zonder-energieverbruik-water-uit-lucht/" target="_blank">press article</a>, this time by Made in Oost-Vlaanderen.</li>
 +
 +
<li class="list-group-item borderless"><b>October 16:</b>
 +
          <ul>
 +
            <li> The Chinese press also did  <a href="http://www.3dhoo.com/news/guowai/26384.html" target="_blank">an article</a> on our project! 
 +
            </li>
 +
            <li>
 +
            Measuring the amount of water that was collected on our collectors.
 +
            <div class="center"><img src="https://static.igem.org/mediawiki/2016/d/d8/Collectors_wegen.jpeg" alt="Water collector version 4" height="384" width="512"></div>
 +
            </li>
 +
          </ul>
 +
        </li>
 +
 +
<li class="list-group-item borderless"><b>October 17:</b>
 +
          <ul>
 +
            <li>
 +
            Running the proof-of-concept experiments with the microscopic slides.
 +
            <div class="center"><img src="https://static.igem.org/mediawiki/2016/6/6c/T--UGent_Belgium--slideshum.jpeg" alt="Water collector version 4" height="384" width="512"></div>
 +
            </li>
 +
          </ul>
 +
        </li>
 +
 +
<li class="list-group-item borderless"><b>October 18:</b>
 +
          <ul>
 +
            <li>
 +
            Measuring the amount of water that was collected on the microscopic slides.
 +
            </li>
 +
 +
            <li>
 +
            Working hard on the deadline for wiki freeze.
 +
<div class="center"><img src="https://static.igem.org/mediawiki/2016/7/76/T--UGent_Belgium--simulab.jpg" alt="simulation lab" width="600">
 +
</div>
 +
            </li>
 +
 +
            <li>
 +
              National television picked up on our project. In the video, you see our translation of the Dutch video.
 +
              <div class="center"><video controls="" style="width: 500px; height:auto;object-fit: contain;">
 +
<source src="https://static.igem.org/mediawiki/2016/8/89/T--UGent_Belgium--vtm_translation.mp4" type="video/mp4" >
 +
</video></div>
 +
            </li>
 +
          </ul>
 +
        </li>
 +
 +
 +
        <li class="list-group-item borderless"><b>October 26:</b> We're off to Boston!</li>
 +
      </ul>
 
     </div>
 
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   </div>
 
   </div>
 
</div>
 
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Latest revision as of 23:14, 19 October 2016

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General notebook


February

  • February 29:
    • Our application for iGEM is sent.
    • A team of four master students (Bram Danneels, Griet De Clercq, Wouter Steyaert and Chari Vandenbussche) is assembled under guidance of PI Wim.

March

  • March 03: We start brainstorming for subjects.
  • March 24: PI's Marjan and Yves join the team.

April

  • April 22: Three subjects are chosen, and we start preparing a presentaion about them.

May

  • May 03: Our iGEM application is officially accepted.
  • May 04: Presentation of our possible subjects for PI Wim and our fellow sudents.
  • May 06: Our team grows, with six phd-students and one adivsor joining.
  • May 17: We sit together for the first time and discuss the different subjects.
  • May 31: We chose our subject! The work gets divided into four work packages, and we divide the work.

June

  • June 15: Fablab was contacted to be a partner for our 3D-printing needs, and they gladly accepted.
  • June 29: We write an abstract on our project, and submit it to our iGEM wiki.

July

  • July 07:
    • Our Facebook and Twitter pages are live.
    • A first draft of a 'sponsor letter' is made.
  • July 18: We booked our stay in Boston.
  • July 19: The 'shape' work package decides to use Autodesk Fusion 360 to shape the water collector.
  • July 22: Prof Dan Fernandez (CSUMB), who performs research on fog catchers, is interested to with us.

August

  • August 01: Our first 3D model of the water collector prototype is ready and printed: a dome with a gutter to collect the water.
    ShapeV1
    ShapeV1Print
  • August 04: We optimized our water collector shape with holes to distribute the water.
    ShapeV2
  • August 15: We created an informational video, showing what our project is about.
  • August 19: We successfully dissolved 0.55g PLA in 8ml DMF at 80-90 °C (68.75 g/L) and precipitated it by pouring the solution into room temperature EtOH (disolol, denatured).
  • August 20: The filament was precipitated and washed with ethanol (cf. 19/09).
  • August 22: We brainstormed about the possibility to stack our collector, but unfortunatly this is not geometrically feasible.
    ShapeV2
  • August 30: We brainstorm on other ways to combine our collectors. Meanwhile, we redsign the collector with only holes in the bottom ridge, and on the top. Also, the inside of the collector is made hollow.

September

  • September 01:
    • The first batch of biotin is delivered: the work on the filament can begin!
      Biotin

    • Steven, our advisor, met up with the EPFL team in Lausanne, Switzerland. They immediately bonded over a barbecue.
      Steven with the Lausanne team
  • September 12: We performed some solubility and precipitation tests on the biotin and determine the optimal precipitation solution composition.
  • September 13:
    • We all got to know the EPFL team better, as they conducted a Skype interview with us.
      Skype interview Lausanne
    • Dewpal has been chosen as name!
    • We came up with yet another design, now with internal water distribution.
  • September 14:
    • We printed three different prototypes of our water collector.
    • We scaled the filament up 10 times. A slight raise in temperature was necessary to dissolve the PLA (145°C instead of 130°C on plate). When precipitating in ethanol, a rather glutinous substance was received on top of some big flakes that could be recovered.
  • September 15:
    • Our crowdfunding page is live!
    • The filament sample (cf. 14/09) was decanted and centrifuged. PLA-biotin was harvested (pellet) and set to dry at 70°C overnight.
  • September 18: We made a temperature and humidity sensor with Raspberry Pi so we can observe the environment while testing our 3D shape.
    Humidity Sensor
  • September 20: The water collector without biotin was tested for its water collecting efficiency.
  • September 21: We met with Winnie from ReaGent.
  • September 23:
    • We met with Deepak from 3dee, who gave us some amazing advice on how to enhance our 3D shape.
    • Our filament group went to Timelab to make a batch of filament using their extruder.
      Making filament at Timelab
  • September 24: We made an experimental set-up with a humidifier and temperature/humidity sensor (cf. 15/09) to test the water collection of our shapes.
    Humidifier set up
  • September 26: We tried our INP, and it works!
  • September 29: Our logo was designed. Our shape design now includes a screw cap to fit the collectors on each other, and on a plastic bottle.

October

  • October 01: We improved the design of our shape again, now with an active surface area that is almost 20 times larger than the previous version.
    Water collector version 4
  • October 02: Yet another change in our design: now with weaving ribbons.
    Water collector version 4
  • October 07: 3Ders published a press article on our project.
  • October 09: We started coating the water collector with biotin. Solvent with PLA and biotin is poured over the water collector and left to dry under the laminar air cabinet.
    Binding biotin with out water collectors
    Water collector version 4
  • October 10: Digital Trends published a press article about our project.
  • October 12: Testing four water collectors with four treatments: INP + INP_NC-mSA2, RFP + INP_NC-mSA2, INP_RC-mSA2 and mGFPuv2-mSA2. Each collector is sawn in half, one half receives a treatment and the other half is the control. The water collectors are placed overnight in the controlled humidified chamber.
    Binding biotin with out water collectors
  • October 13: Another press article, this time by Made in Oost-Vlaanderen.
  • October 16:
    • The Chinese press also did an article on our project!
    • Measuring the amount of water that was collected on our collectors.
      Water collector version 4
  • October 17:
    • Running the proof-of-concept experiments with the microscopic slides.
      Water collector version 4
  • October 18:
    • Measuring the amount of water that was collected on the microscopic slides.
    • Working hard on the deadline for wiki freeze.
      simulation lab
    • National television picked up on our project. In the video, you see our translation of the Dutch video.
  • October 26: We're off to Boston!