Difference between revisions of "Team:IIT-Madras/Parts"

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= Composite Parts =
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We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. These parts include Promoter-RBS composite parts, dual fluorescence reporter devices, RIBOS devices and sgRNA parts. To know more about the characterization and functionality of these parts and their future applications. 
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====== [[Team:IIT-Madras/Composite_Part|Read more...]] ======
  
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= Part Collection =
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We have submitted one part collection for efficient characterization of devices e.g. noise & strength. This part collection contains 12 composite parts. To know more about the characterization and functionality of these parts and their future applications.
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====== [[Team:IIT-Madras/Part_Collection|Read more...]] ======
  
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= Size Characterization =
  
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Following is the gel picture of our constructs, P represents plasmid DNA and D represents plasmid DNA digested with EcoRI-HF and PstI:
  
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<div class="column full_size">
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[[Image:Igemiitmgel1.png|500x350px]]
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[[Image:Igemiitmgel2.png|500x350px]]
  
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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= Table of important parts =
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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{| class="wikitable" style="font-size:0.8em;@media screen and (max-width: 1000px){font-size:0.6em;}"
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! scope="col"| Part Name
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! scope="col"| Part Number
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|-
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| scope="row"| pL-RBS 1
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| [http://parts.igem.org/Part:BBa_K1956001 BBa_K1956001]
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|-
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| scope="row"| pL-RBS 2
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| [http://parts.igem.org/Part:BBa_K1956002 BBa_K1956002]
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|-
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| scope="row"| pL-RBS 3
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| [http://parts.igem.org/Part:BBa_K1956003 BBa_K1956003]
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|-
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| scope="row"| pL-RBS 4
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| [http://parts.igem.org/Part:BBa_K1956004 BBa_K1956004]
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|-
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| scope="row"| pL-RIBOSOFF
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| [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005]
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|-
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| scope="row"| pL-gRNA1_CamR
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| [http://parts.igem.org/Part:BBa_K1956006 BBa_K1956006]
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|-
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| scope="row"| pL-gRNA2_CamR
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| [http://parts.igem.org/Part:BBa_K1956007 BBa_K1956007]
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|-
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| scope="row"| pL-gRNA1_mCherry
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| [http://parts.igem.org/Part:BBa_K1956008 BBa_K1956008]
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|-
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| scope="row"| pL-gRNA2_mCherry
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| [http://parts.igem.org/Part:BBa_K1956009 BBa_K1956009]
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|-
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| scope="row"| pL-gRNA_KanR
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| [http://parts.igem.org/Part:BBa_K1956010 BBa_K1956010]
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|-
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| scope="row"| pL-gRNA1_CamR-DT
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| [http://parts.igem.org/Part:BBa_K1956011 BBa_K1956011]
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|-
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| scope="row"| pL-gRNA1_mCherry-DT
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| [http://parts.igem.org/Part:BBa_K1956012 BBa_K1956012]
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|-
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| scope="row"| pL-gRNA_KanR-DT
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| [http://parts.igem.org/Part:BBa_K1956013 BBa_K1956013]
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|-
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| scope="row"| Noise in Device Kit 3
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| [http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]
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|-
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| scope="row"| Noise in Device kit 4
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| [http://parts.igem.org/Part:BBa_K1956015 BBa_K1956015]
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|-
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| scope="row"| Expression vector for efficient Characterization
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| [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016]
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|-
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| scope="row"| Noise in Device kit 5
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| [http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]
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|-
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| scope="row"| Noise in Device kit 6
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| [http://parts.igem.org/Part:BBa_K1956018 BBa_K1956018]
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|-
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| scope="row"| RIBOSON for mCherry RNA with K1956016
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| [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019]
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|-
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| scope="row"| RIBOSOFF 1 for lacI RNA with K1956016
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| [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020]
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|-
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| scope="row"| RIBOSOFF2_lacIlacI-K1956016
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| [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021]
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|-
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| scope="row"| Noise in Device Kit 1
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| [http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022]
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|-
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| scope="row"| Noise in Device Kit 2
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| [http://parts.igem.org/Part:BBa_K1956023 BBa_K1956023]
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|-
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| scope="row"| LacI expression device
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| [http://parts.igem.org/Part:BBa_K1956034 BBa_K1956034]
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|-
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|}
  
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
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<div class="highlight">
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<groupparts>iGEM2016 Example</groupparts>
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Latest revision as of 23:18, 19 October 2016


Composite Parts

We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. These parts include Promoter-RBS composite parts, dual fluorescence reporter devices, RIBOS devices and sgRNA parts. To know more about the characterization and functionality of these parts and their future applications.

Read more...

Part Collection

We have submitted one part collection for efficient characterization of devices e.g. noise & strength. This part collection contains 12 composite parts. To know more about the characterization and functionality of these parts and their future applications.

Read more...

Size Characterization

Following is the gel picture of our constructs, P represents plasmid DNA and D represents plasmid DNA digested with EcoRI-HF and PstI:

Igemiitmgel1.png Igemiitmgel2.png


Table of important parts

Part Name Part Number
pL-RBS 1 [http://parts.igem.org/Part:BBa_K1956001 BBa_K1956001]
pL-RBS 2 [http://parts.igem.org/Part:BBa_K1956002 BBa_K1956002]
pL-RBS 3 [http://parts.igem.org/Part:BBa_K1956003 BBa_K1956003]
pL-RBS 4 [http://parts.igem.org/Part:BBa_K1956004 BBa_K1956004]
pL-RIBOSOFF [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005]
pL-gRNA1_CamR [http://parts.igem.org/Part:BBa_K1956006 BBa_K1956006]
pL-gRNA2_CamR [http://parts.igem.org/Part:BBa_K1956007 BBa_K1956007]
pL-gRNA1_mCherry [http://parts.igem.org/Part:BBa_K1956008 BBa_K1956008]
pL-gRNA2_mCherry [http://parts.igem.org/Part:BBa_K1956009 BBa_K1956009]
pL-gRNA_KanR [http://parts.igem.org/Part:BBa_K1956010 BBa_K1956010]
pL-gRNA1_CamR-DT [http://parts.igem.org/Part:BBa_K1956011 BBa_K1956011]
pL-gRNA1_mCherry-DT [http://parts.igem.org/Part:BBa_K1956012 BBa_K1956012]
pL-gRNA_KanR-DT [http://parts.igem.org/Part:BBa_K1956013 BBa_K1956013]
Noise in Device Kit 3 [http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]
Noise in Device kit 4 [http://parts.igem.org/Part:BBa_K1956015 BBa_K1956015]
Expression vector for efficient Characterization [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016]
Noise in Device kit 5 [http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]
Noise in Device kit 6 [http://parts.igem.org/Part:BBa_K1956018 BBa_K1956018]
RIBOSON for mCherry RNA with K1956016 [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019]
RIBOSOFF 1 for lacI RNA with K1956016 [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020]
RIBOSOFF2_lacIlacI-K1956016 [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021]
Noise in Device Kit 1 [http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022]
Noise in Device Kit 2 [http://parts.igem.org/Part:BBa_K1956023 BBa_K1956023]
LacI expression device [http://parts.igem.org/Part:BBa_K1956034 BBa_K1956034]