Difference between revisions of "Team:IIT-Madras/Parts"

 
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{{IIT-Madras-Top/CSS}}
 
{{IIT-Madras-Top/CSS}}
  
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= Overview =
 
  
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= Composite Parts =
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We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. These parts include Promoter-RBS composite parts, dual fluorescence reporter devices, RIBOS devices and sgRNA parts. To know more about the characterization and functionality of these parts and their future applications. 
 +
====== [[Team:IIT-Madras/Composite_Part|Read more...]] ======
 +
 +
= Part Collection =
 +
We have submitted one part collection for efficient characterization of devices e.g. noise & strength. This part collection contains 12 composite parts. To know more about the characterization and functionality of these parts and their future applications.
 +
====== [[Team:IIT-Madras/Part_Collection|Read more...]] ======
 +
 +
= Size Characterization =
 +
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Following is the gel picture of our constructs, P represents plasmid DNA and D represents plasmid DNA digested with EcoRI-HF and PstI:
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[[Image:Igemiitmgel1.png|500x350px]]
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[[Image:Igemiitmgel2.png|500x350px]]
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= Table of important parts =
 
{| class="wikitable" style="font-size:0.8em;@media screen and (max-width: 1000px){font-size:0.6em;}"
 
{| class="wikitable" style="font-size:0.8em;@media screen and (max-width: 1000px){font-size:0.6em;}"
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|+List of parts that we have submitted for iGEM 2016
 
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|-
 
 
! scope="col"| Part Name
 
! scope="col"| Part Name
 
! scope="col"| Part Number
 
! scope="col"| Part Number
 
|-
 
|-
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| scope="row"| Lambda pL hybrid promoter with B0030 RBS
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| scope="row"| pL-RBS 1
 
| [http://parts.igem.org/Part:BBa_K1956001 BBa_K1956001]
 
| [http://parts.igem.org/Part:BBa_K1956001 BBa_K1956001]
 
|-
 
|-
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| scope="row"| Lambda pL hybrid promoter with B0031 RBS
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| scope="row"| pL-RBS 2
 
| [http://parts.igem.org/Part:BBa_K1956002 BBa_K1956002]
 
| [http://parts.igem.org/Part:BBa_K1956002 BBa_K1956002]
 
|-
 
|-
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| scope="row"| Lambda pL hybrid promoter with B0032 RBS
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| scope="row"| pL-RBS 3
 
| [http://parts.igem.org/Part:BBa_K1956003 BBa_K1956003]
 
| [http://parts.igem.org/Part:BBa_K1956003 BBa_K1956003]
 
|-
 
|-
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| scope="row"| Lambda pL hybrid promoter with B0034 RBS
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| scope="row"| pL-RBS 4
 
| [http://parts.igem.org/Part:BBa_K1956004 BBa_K1956004]
 
| [http://parts.igem.org/Part:BBa_K1956004 BBa_K1956004]
 
|-
 
|-
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| scope="row"| RIBOSOFF for lacI coding RNA under J23106
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| scope="row"| pL-RIBOSOFF
 
| [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005]
 
| [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005]
 
|-
 
|-
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| scope="row"| gRNA 1 for CamR gene under lambda pL hybrid
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| scope="row"| pL-gRNA1_CamR
 
| [http://parts.igem.org/Part:BBa_K1956006 BBa_K1956006]
 
| [http://parts.igem.org/Part:BBa_K1956006 BBa_K1956006]
 
|-
 
|-
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| scope="row"| gRNA 2 for CamR gene under lambda pL hybrid
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| scope="row"| pL-gRNA2_CamR
 
| [http://parts.igem.org/Part:BBa_K1956007 BBa_K1956007]
 
| [http://parts.igem.org/Part:BBa_K1956007 BBa_K1956007]
 
|-
 
|-
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| scope="row"| gRNA 1 for mCherry gene under lambda pL hybrid
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| scope="row"| pL-gRNA1_mCherry
 
| [http://parts.igem.org/Part:BBa_K1956008 BBa_K1956008]
 
| [http://parts.igem.org/Part:BBa_K1956008 BBa_K1956008]
 
|-
 
|-
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| scope="row"| gRNA 2 for mCherry gene under lambda pL hybrid
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| scope="row"| pL-gRNA2_mCherry
 
| [http://parts.igem.org/Part:BBa_K1956009 BBa_K1956009]
 
| [http://parts.igem.org/Part:BBa_K1956009 BBa_K1956009]
 
|-
 
|-
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| scope="row"| gRNA for KanR gene under lambda pL hybrid
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| scope="row"| pL-gRNA_KanR
 
| [http://parts.igem.org/Part:BBa_K1956010 BBa_K1956010]
 
| [http://parts.igem.org/Part:BBa_K1956010 BBa_K1956010]
 
|-
 
|-
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| scope="row"| gRNA 1 for CamR gene under lambda pL hybrid with terminator
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| scope="row"| pL-gRNA1_CamR-DT
 
| [http://parts.igem.org/Part:BBa_K1956011 BBa_K1956011]
 
| [http://parts.igem.org/Part:BBa_K1956011 BBa_K1956011]
 
|-
 
|-
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| scope="row"| gRNA 1 for mCherry gene under lambda pL hybrid with terminator
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| scope="row"| pL-gRNA1_mCherry-DT
 
| [http://parts.igem.org/Part:BBa_K1956012 BBa_K1956012]
 
| [http://parts.igem.org/Part:BBa_K1956012 BBa_K1956012]
 
|-
 
|-
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| scope="row"| gRNA for KanR gene under lambda pL hybrid with terminator
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| scope="row"| pL-gRNA_KanR-DT
 
| [http://parts.igem.org/Part:BBa_K1956013 BBa_K1956013]
 
| [http://parts.igem.org/Part:BBa_K1956013 BBa_K1956013]
 
|-
 
|-
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| scope="row"| Noise in Device kit 1
+
| scope="row"| Noise in Device Kit 3
 
| [http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]
 
| [http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]
 
|-
 
|-
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| scope="row"| Noise in Device kit 2
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| scope="row"| Noise in Device kit 4
 
| [http://parts.igem.org/Part:BBa_K1956015 BBa_K1956015]
 
| [http://parts.igem.org/Part:BBa_K1956015 BBa_K1956015]
 
|-
 
|-
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| [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016]
 
| [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016]
 
|-
 
|-
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| scope="row"| Noise in Device kit 3
+
| scope="row"| Noise in Device kit 5
 
| [http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]
 
| [http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]
 
|-
 
|-
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| scope="row"| Noise in Device kit 4
+
| scope="row"| Noise in Device kit 6
 
| [http://parts.igem.org/Part:BBa_K1956018 BBa_K1956018]
 
| [http://parts.igem.org/Part:BBa_K1956018 BBa_K1956018]
 
|-
 
|-
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| [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020]
 
| [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020]
 
|-
 
|-
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| scope="row"| RIBOSOFF 2 for lacI RNA with K1956016
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| scope="row"| RIBOSOFF2_lacIlacI-K1956016
 
| [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021]
 
| [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021]
 +
|-
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| scope="row"| Noise in Device Kit 1
 +
| [http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022]
 +
|-
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| scope="row"| Noise in Device Kit 2
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| [http://parts.igem.org/Part:BBa_K1956023 BBa_K1956023]
 
|-
 
|-
 
| scope="row"| LacI expression device
 
| scope="row"| LacI expression device
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|-
 
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|}
 
|}
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= [[Team:IIT-Madras/Composite_Part|Composite Parts]] =
 
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==== [[Team:IIT-Madras/Composite_Part|Read more...]] ====
 
−
 
−
= [[Team:IIT-Madras/Part_Collection|Part Collection]] =
 
−
==== [[Team:IIT-Madras/Part_Collectiont|Read more...]] ====
 
  
 
{{IIT-Madras-Bottom/CSS}}
 
{{IIT-Madras-Bottom/CSS}}

Latest revision as of 23:18, 19 October 2016


Composite Parts

We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. These parts include Promoter-RBS composite parts, dual fluorescence reporter devices, RIBOS devices and sgRNA parts. To know more about the characterization and functionality of these parts and their future applications.

Read more...

Part Collection

We have submitted one part collection for efficient characterization of devices e.g. noise & strength. This part collection contains 12 composite parts. To know more about the characterization and functionality of these parts and their future applications.

Read more...

Size Characterization

Following is the gel picture of our constructs, P represents plasmid DNA and D represents plasmid DNA digested with EcoRI-HF and PstI:

Igemiitmgel1.png Igemiitmgel2.png


Table of important parts

Part Name Part Number
pL-RBS 1 [http://parts.igem.org/Part:BBa_K1956001 BBa_K1956001]
pL-RBS 2 [http://parts.igem.org/Part:BBa_K1956002 BBa_K1956002]
pL-RBS 3 [http://parts.igem.org/Part:BBa_K1956003 BBa_K1956003]
pL-RBS 4 [http://parts.igem.org/Part:BBa_K1956004 BBa_K1956004]
pL-RIBOSOFF [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005]
pL-gRNA1_CamR [http://parts.igem.org/Part:BBa_K1956006 BBa_K1956006]
pL-gRNA2_CamR [http://parts.igem.org/Part:BBa_K1956007 BBa_K1956007]
pL-gRNA1_mCherry [http://parts.igem.org/Part:BBa_K1956008 BBa_K1956008]
pL-gRNA2_mCherry [http://parts.igem.org/Part:BBa_K1956009 BBa_K1956009]
pL-gRNA_KanR [http://parts.igem.org/Part:BBa_K1956010 BBa_K1956010]
pL-gRNA1_CamR-DT [http://parts.igem.org/Part:BBa_K1956011 BBa_K1956011]
pL-gRNA1_mCherry-DT [http://parts.igem.org/Part:BBa_K1956012 BBa_K1956012]
pL-gRNA_KanR-DT [http://parts.igem.org/Part:BBa_K1956013 BBa_K1956013]
Noise in Device Kit 3 [http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]
Noise in Device kit 4 [http://parts.igem.org/Part:BBa_K1956015 BBa_K1956015]
Expression vector for efficient Characterization [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016]
Noise in Device kit 5 [http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]
Noise in Device kit 6 [http://parts.igem.org/Part:BBa_K1956018 BBa_K1956018]
RIBOSON for mCherry RNA with K1956016 [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019]
RIBOSOFF 1 for lacI RNA with K1956016 [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020]
RIBOSOFF2_lacIlacI-K1956016 [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021]
Noise in Device Kit 1 [http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022]
Noise in Device Kit 2 [http://parts.igem.org/Part:BBa_K1956023 BBa_K1956023]
LacI expression device [http://parts.igem.org/Part:BBa_K1956034 BBa_K1956034]