Difference between revisions of "Team:OUC-China/Protocol"

 
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   <title>Protocol</title>
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<li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li>
 
<li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Project">Project</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a>
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<li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li>
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<ul class="dropdown-menu">
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<li><a href="https://2016.igem.org/Team:OUC-China/Description">Description</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Results">Results</a></li>
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</ul>
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</li>
 
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a>
 
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<li><a href="#">Basic part</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Parts">Parts</a></li>
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<li><a href="#">Composite parts</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Basic_Part">Basic part</a></li>
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<li><a href="#">Part collection</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Composite_Part">Composite parts</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Part_Collection">Part collection</a></li>
 
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</li>
 
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Attribution<span class="caret"></span></a>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Human Practice<span class="caret"></span></a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
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<li><a href="#">Support</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Overview</a></li>
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<li><a href="#">Collaboration</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Communicating">Communicating & Improving</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Investigating">Investigating & Promoting</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<span class="caret"></span></a>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<span class="caret"></span></a>
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<ul class="dropdown-menu">
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<li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li>
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</ul>
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</li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<span class="caret"></span></a>
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<ul class="dropdown-menu">
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<li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Software">Software</a></li>
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</ul>
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</li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Attributions">Attributions</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Safety<span class="caret"></span></a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
 
<li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li>
 
<li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li>
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                                                                <li class="active"><a href="#">Protocol</a></li>
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<li class="active"><a href="https://2016.igem.org/Team:OUC-China/Protocol">Protocol</a></li>
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<li><a href="#">Safety</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Safety">Safety</a></li>
 
</ul>
 
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</li>
 
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<li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Human Practice</a></li>
 
 
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                 <div class="img_banner"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--OUC-China--protocol-banner.jpg" class="img-responsive" alt="protocol-banner"></div>
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<h3>1. Elementary Operation</h3>
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<h3>1. Elementary Operation</h3>
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<p>
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<p>
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1) <a href="https://static.igem.org/mediawiki/2016/1/15/T--OUC-China--Luria-Bertani_medium.pdf">Luria-Bertani liquid and Luria-Bertani solid medium</a><br>
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1) <a href="https://static.igem.org/mediawiki/2016/1/15/T--OUC-China--Luria-Bertani_medium.pdf">Luria-Bertani liquid and Luria-Bertani solid medium</a><br>
−
2) <a href="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--Inoculation.pdf">Inoculation</a><br>
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2) <a href="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--Inoculation.pdf">Inoculation</a><br>
−
3) <a href="">Polymerase Chain Reaction</a><br>
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3) <a href="https://static.igem.org/mediawiki/2016/d/dc/T--OUC-China--Polymerase_Chain_Reaction.pdf">Polymerase Chain Reaction</a><br>
−
4) <a href="https://static.igem.org/mediawiki/2016/c/cb/T--OUC-China--Gel_Electrophoresis.pdf">Gel Electrophoresis</a><br>
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4) <a href="https://static.igem.org/mediawiki/2016/c/cb/T--OUC-China--Gel_Electrophoresis.pdf">Gel Electrophoresis</a><br>
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5) <a href="">Restriction Enzyme Digestion</a><br>
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5) <a href="https://static.igem.org/mediawiki/2016/0/00/T--OUC-China--Restriction_Enzyme_Digestion.pdf">Restriction Enzyme Digestion</a><br>
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6) <a href="https://static.igem.org/mediawiki/2016/d/df/T--OUC-China--Gel_Extraction.pdf">Gel Extraction</a><br>
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6) <a href="https://static.igem.org/mediawiki/2016/d/df/T--OUC-China--Gel_Extraction.pdf">Gel Extraction</a><br>
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7) <a href="">Ligation system</a><br>
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7) <a href="https://static.igem.org/mediawiki/2016/1/14/T--OUC-China--Ligation.pdf">Ligation system</a><br>
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8) <a href="">Transformation of <i>E.coli</i></a><br>
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8) <a href="https://static.igem.org/mediawiki/2016/f/fb/T--OUC-China--Transformation_of_Escherichia_coli.pdf">Transformation of <i>E.coli</i></a><br>
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9) <a href="https://static.igem.org/mediawiki/2016/8/84/T--OUC-China--Conservation.pdf">Conservation</a><br>
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9) <a href="https://static.igem.org/mediawiki/2016/8/84/T--OUC-China--Conservation.pdf">Conservation</a><br>
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10) <a href="">Sterilization</a><br>
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10) <a href="https://static.igem.org/mediawiki/2016/c/c8/T--OUC-China--Sterilization.pdf">Sterilization</a><br>
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11) <a href="">Plasmid extraction</a>
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11) <a href="https://static.igem.org/mediawiki/2016/1/1f/T--OUC-China--Plasmid_extraction.pdf">Plasmid extraction</a>
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</p>
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</p>
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<h3>2. Plasmid Construction</h3>
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<h3>2. Plasmid Construction</h3>
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<p>
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<p>
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1) <a href="">Overlap PCR</a><br>
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1) <a href="https://static.igem.org/mediawiki/2016/d/db/T--OUC-China--Gibson_aassembly.pdf">Gibson</a><br>
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2) <a href="">Gibson</a><br>
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2) <a href="https://static.igem.org/mediawiki/2016/6/6a/T--OUC-China--DNA_cassettes.pdf">DNA cassettes</a>
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3) <a href="">DNA cassettes</a>
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</p>
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</p>
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<h3>3. Systems detection</h3>
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<h3>3. Systems detection</h3>
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<p>
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<p>
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1) <a href="https://static.igem.org/mediawiki/2016/b/b7/T--OUC-China--Total_RNA_Extraction.pdf">RNA extraction</a><br>
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1) <a href="">RNA extraction</a><br>
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2) <a href="https://static.igem.org/mediawiki/2016/b/b9/T--OUC-China--Reserve_transcription.pdf">Reserve transcription</a><br>
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2) <a href="">Reserve transcription</a><br>
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3) <a href="https://static.igem.org/mediawiki/2016/7/7a/T--OUC-China--Quantitative_Real-time_PCR.pdf">Quantitative Real-time PCR</a><br>
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3) <a href="">Quantitative Real-time PCR</a><br>
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4) <a href="https://static.igem.org/mediawiki/2016/2/2f/T--OUC-China--Fluorescence_measurement.pdf">Fluorescence measurement protocol</a>
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4) <a href="">Fluorescence measurement protocol</a>
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</p>
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<div class="col-md-5"></div>
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                <br><br><br><br>
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                </div>
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<div class="oucBanner">
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        <h3 class="text-center">Cistrons Concerto</h3>
 
</div>
 
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                <br><br><br><br>
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<div class="row oucInformation">
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<div class="oucBanner">
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<div class="col-md-5">
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<h3 class="text-center">Cistrons Concerto</h3>
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<h3>Thanks:</h3>
 +
<p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p>
 +
<p>2.NEW ENGLAND Biolabs</p>
 +
<p>3.GenScript</p>
 
</div>
 
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<div class="row oucInformation">
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<div class="col-md-5 Contact">
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<div class="col-md-7">
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<h3>Contact us:</h3>
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<b>About</b>
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<p><b>E-mail</b>: oucigem@163.com</p>
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<p>Thanks to:<i><img src="image/ouc-footer-1.fw.png"></i><i><img src="image/ouc-footer-2.fw.png"></i></p>
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<p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p>
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<p>Designed and built by @ Jasmine Chen and @ Zexin Jiao</p>
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<p>We are OUC-iGEM
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<p>Code licensed under Apache License v2.0</p>
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<img src="https://static.igem.org/mediawiki/2016/5/58/T--OUC-China--foot4.png" width="70" height="70" alt="logo-one">
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<img src="https://static.igem.org/mediawiki/2016/9/9b/T--OUC-China--foot5.png" width="70" height="70" alt="logo-two">
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<div class="col-md-5">
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</p>
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<b>Contact us</b>
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<p>E-mail: oucigem@163.com</p>
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<p>Follow us on Facebook@ iGEM OUC<i><img src="image/ouc-footer-3.fw.png"></i></p>
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<p>Find us on Google Map</p>
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−
<p>We are OUC-iGEM<i><img src="image/ouc-footer-4.fw.png"></i><i><img src="image/ouc-footer-5.fw.png"></i><i><img src="image/ouc-footer-6.fw.png"></i></p>
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Latest revision as of 01:05, 20 October 2016

Protocol

protocol-banner

Cistrons Concerto

Thanks:

1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences

2.NEW ENGLAND Biolabs

3.GenScript

Contact us:

E-mail: oucigem@163.com

Designed and built by @ Jasmine Chen and @ Zexin Jiao

We are OUC-iGEM logo-one logo-two