Difference between revisions of "Team:OUC-China/Protocol"
| (7 intermediate revisions by one other user not shown) | |||
| Line 5: | Line 5: | ||
<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no"> | <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no"> | ||
<meta http-equiv="X-UA-Compatible" content="IE=edge"> | <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
| − | <link href="https://2016.igem.org/Team:OUC-China/css/bootstrap-min?action=raw&ctype=text/css | + | <link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/bootstrap-min?action=raw&ctype=text/css"> |
<link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/ouc_wiki?action=raw&ctype=text/css"> | <link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/ouc_wiki?action=raw&ctype=text/css"> | ||
<script type="text/javascript" src="https://2016.igem.org/Team:OUC-China/js/jquery?action=raw&ctype=text/javascript"></script> | <script type="text/javascript" src="https://2016.igem.org/Team:OUC-China/js/jquery?action=raw&ctype=text/javascript"></script> | ||
| Line 43: | Line 43: | ||
<body> | <body> | ||
<div class="container"> | <div class="container"> | ||
| − | <nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation"> | + | <nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation"> |
<div class="container"> | <div class="container"> | ||
<div class="navbar-header"> | <div class="navbar-header"> | ||
| Line 58: | Line 58: | ||
<ul class="nav navbar-nav" id="oucNav"> | <ul class="nav navbar-nav" id="oucNav"> | ||
<li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li> | ||
| − | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a> |
| − | + | <ul class="dropdown-menu"> | |
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Description">Description</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Results">Results</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a> | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| Line 68: | Line 74: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Human Practice<span class="caret"></span></a> | |
| − | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#"> | + | |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| − | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Overview</a></li> |
| − | + | <li><a href="https://2016.igem.org/Team:OUC-China/Communicating">Communicating & Improving</a></li> | |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Investigating">Investigating & Promoting</a></li> |
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<span class="caret"></span></a> | |
| − | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#"> | + | |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| − | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li> |
<li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<span class="caret"></span></a> | ||
| + | <ul class="dropdown-menu"> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Software">Software</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Attributions">Attributions</a></li> | ||
| + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Safety<span class="caret"></span></a> | ||
| + | <ul class="dropdown-menu"> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li> | ||
| + | <li class="active"><a href="https://2016.igem.org/Team:OUC-China/Protocol">Protocol</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Safety">Safety</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
| Line 90: | Line 109: | ||
<div class="img_banner"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--OUC-China--protocol-banner.jpg" class="img-responsive" alt="protocol-banner"></div> | <div class="img_banner"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--OUC-China--protocol-banner.jpg" class="img-responsive" alt="protocol-banner"></div> | ||
<div style="background:#EFF2F6;"> | <div style="background:#EFF2F6;"> | ||
| − | + | <br> | |
| − | + | <br> | |
| − | + | <br> | |
| − | + | <br> | |
| − | + | <div class="row"> | |
| − | + | <div class="col-md-6 oucprotocol col-md-push-3" style="margin:0 auto;max-width:90%;border:2px solid #c0c0c0;border-radius:10px;background:#fff;"> | |
| − | + | <h3>1. Elementary Operation</h3> | |
| − | + | <p> | |
| − | + | 1) <a href="https://static.igem.org/mediawiki/2016/1/15/T--OUC-China--Luria-Bertani_medium.pdf">Luria-Bertani liquid and Luria-Bertani solid medium</a><br> | |
| − | + | 2) <a href="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--Inoculation.pdf">Inoculation</a><br> | |
| − | + | 3) <a href="https://static.igem.org/mediawiki/2016/d/dc/T--OUC-China--Polymerase_Chain_Reaction.pdf">Polymerase Chain Reaction</a><br> | |
| − | + | 4) <a href="https://static.igem.org/mediawiki/2016/c/cb/T--OUC-China--Gel_Electrophoresis.pdf">Gel Electrophoresis</a><br> | |
| − | + | 5) <a href="https://static.igem.org/mediawiki/2016/0/00/T--OUC-China--Restriction_Enzyme_Digestion.pdf">Restriction Enzyme Digestion</a><br> | |
| − | + | 6) <a href="https://static.igem.org/mediawiki/2016/d/df/T--OUC-China--Gel_Extraction.pdf">Gel Extraction</a><br> | |
| − | + | 7) <a href="https://static.igem.org/mediawiki/2016/1/14/T--OUC-China--Ligation.pdf">Ligation system</a><br> | |
| − | + | 8) <a href="https://static.igem.org/mediawiki/2016/f/fb/T--OUC-China--Transformation_of_Escherichia_coli.pdf">Transformation of <i>E.coli</i></a><br> | |
| − | + | 9) <a href="https://static.igem.org/mediawiki/2016/8/84/T--OUC-China--Conservation.pdf">Conservation</a><br> | |
| − | + | 10) <a href="https://static.igem.org/mediawiki/2016/c/c8/T--OUC-China--Sterilization.pdf">Sterilization</a><br> | |
| − | + | 11) <a href="https://static.igem.org/mediawiki/2016/1/1f/T--OUC-China--Plasmid_extraction.pdf">Plasmid extraction</a> | |
| − | + | </p> | |
| − | + | <h3>2. Plasmid Construction</h3> | |
| − | + | <p> | |
| − | + | 1) <a href="https://static.igem.org/mediawiki/2016/d/db/T--OUC-China--Gibson_aassembly.pdf">Gibson</a><br> | |
| − | + | 2) <a href="https://static.igem.org/mediawiki/2016/6/6a/T--OUC-China--DNA_cassettes.pdf">DNA cassettes</a> | |
| − | + | </p> | |
| − | + | <h3>3. Systems detection</h3> | |
| − | + | <p> | |
| − | + | 1) <a href="https://static.igem.org/mediawiki/2016/b/b7/T--OUC-China--Total_RNA_Extraction.pdf">RNA extraction</a><br> | |
| − | + | 2) <a href="https://static.igem.org/mediawiki/2016/b/b9/T--OUC-China--Reserve_transcription.pdf">Reserve transcription</a><br> | |
| − | + | 3) <a href="https://static.igem.org/mediawiki/2016/7/7a/T--OUC-China--Quantitative_Real-time_PCR.pdf">Quantitative Real-time PCR</a><br> | |
| − | + | 4) <a href="https://static.igem.org/mediawiki/2016/2/2f/T--OUC-China--Fluorescence_measurement.pdf">Fluorescence measurement protocol</a> | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | </div> | |
<br><br><br><br> | <br><br><br><br> | ||
</div> | </div> | ||
| − | |||
<div class="oucBanner"> | <div class="oucBanner"> | ||
<h3 class="text-center">Cistrons Concerto</h3> | <h3 class="text-center">Cistrons Concerto</h3> | ||
| Line 133: | Line 151: | ||
<div class="col-md-1"></div> | <div class="col-md-1"></div> | ||
<div class="col-md-5"> | <div class="col-md-5"> | ||
| − | <h3> | + | <h3>Thanks:</h3> |
| − | + | <p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p> | |
| − | <p> | + | <p>2.NEW ENGLAND Biolabs</p> |
| − | <p> | + | <p>3.GenScript</p> |
| − | <p> | + | |
</div> | </div> | ||
| − | + | <div class="col-md-5 Contact"> | |
| − | <div class="col-md-5"> | + | |
<h3>Contact us:</h3> | <h3>Contact us:</h3> | ||
| − | < | + | <p><b>E-mail</b>: oucigem@163.com</p> |
| − | + | <p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p> | |
| − | <p> | + | <p>We are OUC-iGEM |
| − | + | <img src="https://static.igem.org/mediawiki/2016/5/58/T--OUC-China--foot4.png" width="70" height="70" alt="logo-one"> | |
| − | <p>We are OUC-iGEM<img src="https://static.igem.org/mediawiki/2016/5/58/T--OUC-China--foot4.png" alt="logo-one"><img src="https://static.igem.org/mediawiki/2016/9/9b/T--OUC-China--foot5.png" alt="logo-two"></p> | + | <img src="https://static.igem.org/mediawiki/2016/9/9b/T--OUC-China--foot5.png" width="70" height="70" alt="logo-two"> |
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div | + | <div class="oucBottom"> |
</div> | </div> | ||
Latest revision as of 01:05, 20 October 2016
1. Elementary Operation
1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction
2. Plasmid Construction
1) Gibson
2) DNA cassettes
3. Systems detection
1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol
Cistrons Concerto
Thanks:
1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
2.NEW ENGLAND Biolabs
3.GenScript
Contact us:
E-mail: oucigem@163.com
Designed and built by @ Jasmine Chen and @ Zexin Jiao
We are OUC-iGEM
