Difference between revisions of "Team:OUC-China/Protocol"
| (4 intermediate revisions by one other user not shown) | |||
| Line 60: | Line 60: | ||
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a> | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| − | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Description">Description</a></li> |
<li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li> | ||
<li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li> | ||
| Line 77: | Line 77: | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Overview</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Overview</a></li> | ||
| − | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Communicating">Communicating & Improving</a></li> |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Investigating">Investigating & Promoting</a></li> |
<li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li> | ||
</ul> | </ul> | ||
| Line 145: | Line 145: | ||
<br><br><br><br> | <br><br><br><br> | ||
</div> | </div> | ||
| − | |||
<div class="oucBanner"> | <div class="oucBanner"> | ||
<h3 class="text-center">Cistrons Concerto</h3> | <h3 class="text-center">Cistrons Concerto</h3> | ||
| Line 152: | Line 151: | ||
<div class="col-md-1"></div> | <div class="col-md-1"></div> | ||
<div class="col-md-5"> | <div class="col-md-5"> | ||
| − | <h3>Thanks</h3 | + | <h3>Thanks:</h3> |
| − | + | <p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p> | |
| − | <p | + | <p>2.NEW ENGLAND Biolabs</p> |
| − | <p | + | <p>3.GenScript</p> |
</div> | </div> | ||
<div class="col-md-5 Contact"> | <div class="col-md-5 Contact"> | ||
<h3>Contact us:</h3> | <h3>Contact us:</h3> | ||
| − | |||
<p><b>E-mail</b>: oucigem@163.com</p> | <p><b>E-mail</b>: oucigem@163.com</p> | ||
<p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p> | <p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p> | ||
Latest revision as of 01:05, 20 October 2016
1. Elementary Operation
1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction
2. Plasmid Construction
1) Gibson
2) DNA cassettes
3. Systems detection
1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol
Cistrons Concerto
Thanks:
1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
2.NEW ENGLAND Biolabs
3.GenScript
Contact us:
E-mail: oucigem@163.com
Designed and built by @ Jasmine Chen and @ Zexin Jiao
We are OUC-iGEM
