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− | {{William_and_Mary}} | + | {{Team:William_and_Mary/CSS_RESET}} |
− | + | {{Team:William_and_Mary/CSS_FIX}} | |
+ | {{Team:William_and_Mary/SOURCE}} | ||
+ | {{Team:William_and_Mary/HEADER2}} | ||
+ | <!--website by John Mitchell, iGEM 2016 | ||
+ | Bootstrap template by creative-tim--> | ||
+ | <html lang="en"> | ||
+ | <cfheader name="X-XSS-Protection" value="1"> | ||
+ | |||
+ | <div class="wrapper"> | ||
+ | |||
+ | <div class="section section-numbers-2" id="numbers2" style="padding-top: 0px !important; border-top: 0px !important; margin-top: 0px !important;"> | ||
+ | <div class="parallaxWM pattern-image"> | ||
+ | <img alt="..." src="https://static.igem.org/mediawiki/2016/c/ce/T--William_and_Mary--rubik-background.jpg"/> | ||
+ | </div> | ||
+ | <div class="container"> | ||
+ | <div class="col-md-8 col-md-offset-2 text-center"> | ||
+ | <div class="description"> | ||
+ | <div class="title"> | ||
+ | <h2 style='padding-top: 40px; font-family: "Verlag-Book"; font-size: 50px;'> | ||
+ | Basic Part | ||
+ | </h2> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="clearfix"></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="section section-we-are-2"> | ||
+ | <div class="text-area"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-4"> | ||
+ | <div class="title add-animation-stopped"> | ||
+ | <p class='h2WM' style='padding-left:70px; padding-top: 0px;'> | ||
+ | Motivation | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-7 col-md-offset-1" style='padding-top: 0px;'> | ||
+ | <div class="description add-animation-stopped animation-1"> | ||
+ | <p class='large' style="color:#A9A9A9;"> | ||
+ | Bba_K0026550 is a decoy binding array containing 85 TetO repeat sequences, and was derived from an addgene plasmid from Finney-Manchester et al. (2013) (“Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM). This part can be used to shift the transfer function of an aTC inducible promoter to the left (Figure 1). See decoy binding array page for more information. </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="description"> | ||
+ | <p class='large' style="padding-left:70px;"> | ||
+ | Figure 1: Population level FACs data comparing the relative fluorescence of a pTet sfGFP and TetR reporter with and without a tetO binding array. While the data is noisy, it is clear that the inflection point of the circuit with the binding array has shifted to the left as expected. Additionally, both circuits experienced a decrease in fluorescence at higher aTC concentrations, this is likely due to the toxicity of aTC in high concentrations. (See binding array page for more details) | ||
− | <p> | + | </p> |
− | + | ||
− | </ | + | </p> |
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- | ||
+ | <div id="carousel"> | ||
+ | <div id="section-we-are-1" class="carousel slide add-animation-stopped-1" data-interval="20000"> | ||
+ | <div class="carousel-inner pattern-image animate"> | ||
+ | <div class="item active"> | ||
+ | <img alt="..." class="full-image" src="assets/img/builder/projects/project.jpg"> | ||
+ | </div> | ||
+ | <div class="item"> | ||
+ | <img alt="..." class="full-image" src="assets/img/builder/projects/project.jpg"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <a class="left carousel-control" href="#section-we-are-1" data-slide="prev"><span class="fa fa-angle-left hidden-md hidden-lg"></span></a> | ||
+ | <a class="right carousel-control" href="#section-we-are-1" data-slide="next"><span class="fa fa-angle-right hidden-ms hidden-lg"></span></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | --> | ||
+ | </div> | ||
+ | </html> | ||
− | + | {{Team:William_and_Mary/JS_SOURCE2}} | |
− | + | {{Team:William_and_Mary/FOOTER_SMALL}} | |
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Revision as of 02:11, 20 October 2016
Motivation
Bba_K0026550 is a decoy binding array containing 85 TetO repeat sequences, and was derived from an addgene plasmid from Finney-Manchester et al. (2013) (“Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM). This part can be used to shift the transfer function of an aTC inducible promoter to the left (Figure 1). See decoy binding array page for more information.
Figure 1: Population level FACs data comparing the relative fluorescence of a pTet sfGFP and TetR reporter with and without a tetO binding array. While the data is noisy, it is clear that the inflection point of the circuit with the binding array has shifted to the left as expected. Additionally, both circuits experienced a decrease in fluorescence at higher aTC concentrations, this is likely due to the toxicity of aTC in high concentrations. (See binding array page for more details)
Basic Part