Difference between revisions of "Team:Manchester/Parts"

 
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<br><br>
 
<h1 class="title11">Parts</h1>
 
<h1 class="title11">Parts</h1>
  
 
<center>
 
<center>
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<table cellpadding="5" class="auto-style1">
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<table cellpadding="5" style="margin-bottom:150px;" class="auto-style1">
 
<tr>
 
<tr>
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<th style="border:1px black solid;">BioBrick</th>
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<th style="border:1px black solid;background: #add8e6;">BioBrick</th>
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<th style="border:1px black solid;">Link</th>
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<th style="border:1px black solid;background: #add8e6;">Link</th>
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<th style="border:1px black solid;">Part Name</th>
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<th style="border:1px black solid;background: #add8e6;">Part Name</th>
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<th style="border:1px black solid;">Description</th>
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<th style="border:1px black solid;background: #add8e6;">Description</th>
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<th style="border:1px black solid;">Part Type</th>
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<th style="border:1px black solid;background: #add8e6;">Part Type</th>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td style="border:1px black solid;">BBa_K2092000</td>
 
<td style="border:1px black solid;">BBa_K2092000</td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092000" target="_blank"> http://parts.igem.org/Part:BBa_K2092000</td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092000" target="_blank"> http://parts.igem.org/Part:BBa_K2092000</td>
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<td style="border:1px black solid;">AOx without RBS</td>
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<td style="border:1px black solid;">AOx (without RBS)</td>
 
<td style="border:1px black solid;">
 
<td style="border:1px black solid;">
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This part encodes for the  AOx1 protein from Pichia Pastoris. This enzyme oxidises primary alcohols to produce aldehydes and hydrogen peroxide. It is designed with an NDeI restriction site between the prefix and the start of the AOx gene.  
+
This part encodes for the  AOx1 protein from <i>Pichia pastoris</i>. This enzyme oxidises primary alcohols to produce aldehydes and hydrogen peroxide. It is designed with an NdeI restriction site between the prefix and the start of the AOx gene.  
 
</td>
 
</td>
 
<td style="border:1px black solid;">Basic</td>
 
<td style="border:1px black solid;">Basic</td>
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<td style="border:1px black solid;">BBa_K2092001</td>
 
<td style="border:1px black solid;">BBa_K2092001</td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092001" target="_blank">http://parts.igem.org/Part:BBa_K2092001 </a></td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092001" target="_blank">http://parts.igem.org/Part:BBa_K2092001 </a></td>
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<td style="border:1px black solid;">alcR without RBS/scar</td>
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<td style="border:1px black solid;">alcR (without RBS/scar)</td>
 
<td style="border:1px black solid;">This part encodes for AlcR protein, an ethanol-activated transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine. </td>
 
<td style="border:1px black solid;">This part encodes for AlcR protein, an ethanol-activated transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine. </td>
 
<td style="border:1px black solid;">Basic</td>
 
<td style="border:1px black solid;">Basic</td>
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<td style="border:1px black solid;">BBa_K2092002</td>
 
<td style="border:1px black solid;">BBa_K2092002</td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092002 " target="_blank">http://parts.igem.org/Part:BBa_K2092002 </a></td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092002 " target="_blank">http://parts.igem.org/Part:BBa_K2092002 </a></td>
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<td style="border:1px black solid;">alcA 1<sup>P</sup></td>
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<td style="border:1px black solid;">P<i>alcA</i></td>
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<td style="border:1px black solid;">This part contains the ethanol/alcR regulated  alcA 1 promoter, the native variant of alcA promoter from Aspergillus nidulans which tightly regulates gene expression. It is an improved BioBrick from 2011 iGEM DTU-Denmark <a href="http://parts.igem.org/Part:BBa_K678001" target="_blank">BBa_K678001</a> </td>
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<td style="border:1px black solid;">This part contains the ethanol/alcR regulated  alcA promoter, the native variant of alcA promoter from Aspergillus nidulans which tightly regulates gene expression. It is an improved BioBrick from <a href="http://parts.igem.org/Part:BBa_K678001" target="_blank"> iGEM DTU-Denmark 2011</a> </td>
 
<td style="border:1px black solid;">Basic</td>
 
<td style="border:1px black solid;">Basic</td>
 
</tr>
 
</tr>
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<td style="border:1px black solid;">BBa_K2092003</td>
 
<td style="border:1px black solid;">BBa_K2092003</td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank">http://parts.igem.org/Part:BBa_K2092003 </a></td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank">http://parts.igem.org/Part:BBa_K2092003 </a></td>
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<td style="border:1px black solid;">alcA 2<sup>P</sup></td>
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<td style="border:1px black solid;">P<i>alcA(var)</i></td>
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<td style="border:1px black solid;">This part contains the ethanol/alcR regulated alcA 2 promoter, an engineered variant of the alcA promoter from Aspergillus nidulans which tightly regulates gene expression.  The lack of one binding site on this promoter is suggested to have 70% decrease in promoter strength in comparison to the native <a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank">BBa_K2092002</a>.</td>
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<td style="border:1px black solid;">This part contains the ethanol/alcR regulated alcA promoter, an engineered variant of the alcA promoter from Aspergillus nidulans which tightly regulates gene expression.  The lack of one binding site on this promoter is suggested to have 70% decrease in promoter strength in comparison to the native <a href="http://parts.igem.org/Part:BBa_K2092002" target="_blank"> P<i>alcA</i></a>.</td>
 
<td style="border:1px black solid;">Basic</td>
 
<td style="border:1px black solid;">Basic</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td style="border:1px black solid;">BBa_K2092004</td>
 
<td style="border:1px black solid;">BBa_K2092004</td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092004" target="_blank">http://parts.igem.org/Part:BBa_K2092004 or 2005</a></td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092004" target="_blank">http://parts.igem.org/Part:BBa_K2092004</a></td>
 
<td style="border:1px black solid;">alcR (incl. RBS)</td>
 
<td style="border:1px black solid;">alcR (incl. RBS)</td>
−
<td style="border:1px black solid;">This part is a twin of <a href="http://parts.igem.org/Part:BBa_K2092001" target="_blank">BBa_K2092001</a>  with an additional < href="http://parts.igem.org/Part:BBa_J34801" target="_blank">RBS BBa_J34801</a>  being synthesized upstream of the start codon.  This part encodes for AlcR protein, an ethanol-activated transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine.</td>
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<td style="border:1px black solid;">This part is a twin of <a href="http://parts.igem.org/Part:BBa_K2092001" target="_blank">BBa_K2092001</a>  with an additional <a href="http://parts.igem.org/Part:BBa_J34801" target="_blank"> RBS </a>  upstream of the start codon.  This part encodes for AlcR protein, an ethanol-activated transcriptional factor from <i>Aspergillus nidulans</i>. It binds to <a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank">alcA</a> and aldA promoters to induce expression of proteins in the presence of ethanol or threonine.</td>
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<td style="border:1px black solid;">Composite</td>
+
−
</tr>
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−
<tr>
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−
<td style="border:1px black solid;">BBa_K2092005</td>
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−
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092009 " target="_blank">http://parts.igem.org/Part:BBa_K2092009</a> </td>
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−
<td style="border:1px black solid;">alcA2+RBS+amilCP</td>
+
−
<td style="border:1px black solid;">This part consists of alcA2 (<a href="http://parts.igem.org/Part:BBa_K2092003" target="_balnk">BBa_K2092003</a>) tagged with a blue chromoprotein, which makes it capable to produce blue colour upon the binding of its ethanol-activated transcription factor AlcR.</td>
+
 
<td style="border:1px black solid;">Composite</td>
 
<td style="border:1px black solid;">Composite</td>
 
</tr>
 
</tr>
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<td style="border:1px black solid;">BBa_K2092006</td>
 
<td style="border:1px black solid;">BBa_K2092006</td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092006" target="_balnk">http://parts.igem.org/Part:BBa_K2092006</a></td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092006" target="_balnk">http://parts.igem.org/Part:BBa_K2092006</a></td>
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<td style="border:1px black solid;">CP3+RBS+amilCP</td>
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<td style="border:1px black solid;">Pcon+RBS+amilCP</td>
−
<td>This part consists of a constitutive promoter (J23110), RBS (B0034) and blue chromoprotein (amilCP).  Suitable to be used as a reporter.</td>
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<td style="border:1px black solid;">This part consists of a <a href="http://parts.igem.org/Part:BBa_J23110" target="_blank"> constitutive promoter</a>, <a href="http://parts.igem.org/Part:BBa_B0034" target="_blank"> RBS</a> and <a href="http://parts.igem.org/Part:BBa_K592009" target="_blank"> blue chromoprotein amilCP</a>.  Suitable to be used as a reporter.</td>
−
<td>Composite</td>
+
−
</tr>
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−
<tr>
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−
<td>BBa_K2092007</td>
+
−
<td><a href="http://parts.igem.org/Part:BBa_K2092007" target="_blank">http://parts.igem.org/Part:BBa_K2092007</a></td>
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−
<td>CP3+RBS+amilGFP</td>
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−
<td style="border:1px black solid;">This part consists of a constitutive promoter (J23110), RBS (B0034) and yellow chromoprotein (amilGFP).  Suitable to be used as a reporter.</td>
+
 
<td style="border:1px black solid;">Composite</td>
 
<td style="border:1px black solid;">Composite</td>
 
</tr>
 
</tr>
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<td style="border:1px black solid;">BBa_K2092008</td>
 
<td style="border:1px black solid;">BBa_K2092008</td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092008" target="_blank">http://parts.igem.org/Part:BBa_K2092008</a></td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092008" target="_blank">http://parts.igem.org/Part:BBa_K2092008</a></td>
−
<td style="border:1px black solid;">CP3+RBS+alcR</td>
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<td style="border:1px black solid;">Pcon+RBS+alcR</td>
−
<td style="border:1px black solid;">This part consists of a constitutive promoter (J23110), RBS (B0034) and alcR.  Suitable to be used to measure the promoter strengths of alcA/aldA.</td>
+
<td style="border:1px black solid;">This part consists of a <a href="http://parts.igem.org/Part:BBa_J23110" target="_blank"> constitutive promoter</a>, <a href="http://parts.igem.org/Part:BBa_B0034" target="_blank"> RBS</a> and <a href="http://parts.igem.org/Part:BBa_K2092001" target="_blank"> alcR</a>.  Suitable to be used to measure the promoter strengths of <a href="http://partsregistry.org/Part:BBa_K592002" target="_blank">alcA</a>/aldA.</td>
 
<td style="border:1px black solid;">Composite</td>
 
<td style="border:1px black solid;">Composite</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td style="border:1px black solid;">BBa_K2092009</td>
 
<td style="border:1px black solid;">BBa_K2092009</td>
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<td style="border:1px black solid;">&nbsp;</td>
+
<td style="border:1px black solid;"><a href="
−
<td style="border:1px black solid;">&nbsp;</td>
+
http://parts.igem.org/Part:BBa_K2092009"target="_blank">http://parts.igem.org/Part:BBa_K2092009</a></td>
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<td style="border:1px black solid;">&nbsp;</td>
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<td style="border:1px black solid;">P<i>alcA</i>(var)+RBS+amilCP</td>
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<td style="border:1px black solid;">&nbsp;</td>
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<td style="border:1px black solid;">
 +
This part consists of <a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank">P<i>alcA</i>(var)</a> tagged with <a href="http://partsregistry.org/Part:BBa_K592009" target="_blank">blue chromoprotein amilCP</a>, which makes it capable to produce blue colour upon the binding of its ethanol-activated transcription factor <a href="http://partsregistry.org/Part:BBa_K592001" target="_blank">AlcR</a>.</td>
 +
<td style="border:1px black solid;">Composite</td>
 
</tr>
 
</tr>
 
</table>
 
</table>
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<div class="floatleft1 project">
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<a class="projectlink" href="https://2016.igem.org/Team:Manchester/Achievement"><< Achievement Overview</a>
 +
</div>
  
 
</html>
 
</html>
 
{{Manchester/CSS/footer}}
 
{{Manchester/CSS/footer}}

Latest revision as of 02:24, 20 October 2016

Manchester iGEM 2016


Parts

BioBrick Link Part Name Description Part Type
BBa_K2092000 http://parts.igem.org/Part:BBa_K2092000 AOx (without RBS) This part encodes for the AOx1 protein from Pichia pastoris. This enzyme oxidises primary alcohols to produce aldehydes and hydrogen peroxide. It is designed with an NdeI restriction site between the prefix and the start of the AOx gene. Basic
BBa_K2092001 http://parts.igem.org/Part:BBa_K2092001 alcR (without RBS/scar) This part encodes for AlcR protein, an ethanol-activated transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine. Basic
BBa_K2092002 http://parts.igem.org/Part:BBa_K2092002 PalcA This part contains the ethanol/alcR regulated alcA promoter, the native variant of alcA promoter from Aspergillus nidulans which tightly regulates gene expression. It is an improved BioBrick from iGEM DTU-Denmark 2011 Basic
BBa_K2092003 http://parts.igem.org/Part:BBa_K2092003 PalcA(var) This part contains the ethanol/alcR regulated alcA promoter, an engineered variant of the alcA promoter from Aspergillus nidulans which tightly regulates gene expression. The lack of one binding site on this promoter is suggested to have 70% decrease in promoter strength in comparison to the native PalcA. Basic
BBa_K2092004 http://parts.igem.org/Part:BBa_K2092004 alcR (incl. RBS) This part is a twin of BBa_K2092001 with an additional RBS upstream of the start codon. This part encodes for AlcR protein, an ethanol-activated transcriptional factor from Aspergillus nidulans. It binds to alcA and aldA promoters to induce expression of proteins in the presence of ethanol or threonine. Composite
BBa_K2092006 http://parts.igem.org/Part:BBa_K2092006 Pcon+RBS+amilCP This part consists of a constitutive promoter, RBS and blue chromoprotein amilCP. Suitable to be used as a reporter. Composite
BBa_K2092008 http://parts.igem.org/Part:BBa_K2092008 Pcon+RBS+alcR This part consists of a constitutive promoter, RBS and alcR. Suitable to be used to measure the promoter strengths of alcA/aldA. Composite
BBa_K2092009 http://parts.igem.org/Part:BBa_K2092009 PalcA(var)+RBS+amilCP This part consists of PalcA(var) tagged with blue chromoprotein amilCP, which makes it capable to produce blue colour upon the binding of its ethanol-activated transcription factor AlcR. Composite
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